Hepatitis B virus subgenotype A1, occurrence of subgenotype D4, and S gene mutations among voluntary blood donors in Kenya.

Abstract:

:Kenya is one of the high endemic zones for hepatitis B virus (HBV) infection. The consensuses on prevalence of the HBV genotypes and the existence of their variants have not been fully established in Kenya. Hence, there is a need to further monitor the diversity of HBV. This study aimed to extend the current molecular and epidemiological information about the geographical distribution of HBV genotypes and subgenotypes, as well as to describe the hepatitis B surface antigen (HBsAg) variants circulating in different Regional Blood Transfusion Centres of Kenya. A total of 32 HBsAg positive blood units from five different blood transfusion centers in Kenya were used in the study. The HBV DNA preS/S-gene was amplified and sequenced. Alignments of S gene were applied using reference sequence from GeneBank. Phylogenetic analysis was performed using the MEGAv4.0 software with the neighbor-joining and maximum composite likelihood methods. Twenty-one plasma samples (65.6%) were DNA positive and were successfully sequenced. Eighteen out of the twenty-one isolates (85.7%) belonged to subgenotype A1 Afro-Asian: six were from Nairobi, four from Kisumu, two from Embu, and three each from Eldoret and Mombasa. The other three strains (14.3%, 3/21) belonged to subgenotype D4 from Mombasa. The HBsAg mutations were detected in nine isolates (42.9%, 9/21). The HBV/A1 and HBV/D4 are dominant among blood donors in Kenya. This demonstrates that continuous monitoring of the HBV diversity would help reveal circulating genotypes and subgenotypes as well as mutants of clinical significance in Kenya.

journal_name

Virus Genes

journal_title

Virus genes

authors

Kwange SO,Budambula NL,Kiptoo MK,Okoth F,Ochwoto M,Oduor M,Kimotho JH

doi

10.1007/s11262-013-0976-1

subject

Has Abstract

pub_date

2013-12-01 00:00:00

pages

448-55

issue

3

eissn

0920-8569

issn

1572-994X

journal_volume

47

pub_type

杂志文章
  • Variability of E2 protein-coding sequences of bovine viral diarrhea virus in Polish cattle.

    abstract::Bovine viral diarrhea virus (BVDV) belongs to the Pestivirus genus of the Flaviviridae family and has worldwide distribution, being one of the main causes of economic losses in cattle raising. The genome of pestiviruses is a single strand of positive-sense RNA with a length of 12.3 kb, which encodes one open reading f...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-020-01756-2

    authors: Mirosław P,Polak MP

    更新日期:2020-08-01 00:00:00

  • Reactivation from quiescence does not coincide with a global induction of herpes simplex virus type 1 transactivators.

    abstract::Herpes simplex virus type 1 (HSV-1) reactivates from a small fraction of latently infected neurons in vivo and neuronally differentiated (ND), quiescently infected (QIF)-PC12 cells in vitro. This may be the result of reactivation initiating in only a few cells, or reactivation followed by premature termination of the ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-005-0052-6

    authors: Danaher RJ,Jacob RJ,Miller CS

    更新日期:2006-10-01 00:00:00

  • Short communication: isolation and phylogenetic analysis of an avian-origin H3N2 canine influenza virus in dog shelter, China.

    abstract::A H3N2 canine influenza virus, A/canine/Guangdong/3/2011 (H3N2), was isolated from roaming dogs in rural China. Sequence and phylogenetic analysis of eight gene segments revealed that the A/canine/Guangdong/3/2011 (H3N2) was most similar to a recent H3N2 canine influenza virus isolated in cats from South Korea, which ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-0882-6

    authors: Su S,Yuan Z,Chen J,Xie J,Li H,Huang Z,Zhang M,Du G,Chen Z,Tu L,Zou Y,Miao J,Wang H,Jia K,Li S

    更新日期:2013-06-01 00:00:00

  • Complete genome sequence of a novel Plum pox virus strain W isolate determined by 454 pyrosequencing.

    abstract::The near-complete (99.7 %) genome sequence of a novel Russian Plum pox virus (PPV) isolate Pk, belonging to the strain Winona (W), has been determined by 454 pyrosequencing with the exception of the thirty-one 5'-terminal nucleotides. This region was amplified using 5'RACE kit and sequenced by the Sanger method. Genom...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-0946-7

    authors: Sheveleva A,Kudryavtseva A,Speranskaya A,Belenikin M,Melnikova N,Chirkov S

    更新日期:2013-10-01 00:00:00

  • Correction to: Molecular analysis of maize (Zea mays L.)-infecting mastreviruses in Ethiopia reveals marked diversity of virus genomes and a novel species.

    abstract::The original version of this article unfortunately contained errors in the "Results" section. ...

    journal_title:Virus genes

    pub_type: 已发布勘误

    doi:10.1007/s11262-019-01662-2

    authors: Guadie D,Tesfaye K,Knierim D,Winter S,Abraham A

    更新日期:2019-06-01 00:00:00

  • Genetic diversity of Sugarcane bacilliform virus isolates infecting Saccharum spp. in India.

    abstract::Sugarcane bacilliform virus (SCBV), which causes leaf freckle in sugarcane, is a member of the genus Badnavirus. Studies were conducted to characterize SCBV in Saccharum officinarum germplasm and cultivated varieties in India by sequencing the complete genomes of five isolates. Genome lengths ranged from 7,553 to 7,88...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-0890-6

    authors: Karuppaiah R,Viswanathan R,Kumar VG

    更新日期:2013-06-01 00:00:00

  • New cases of Mokola virus infection in South Africa: a genotypic comparison of Southern African virus isolates.

    abstract::Mokola virus, one of the six genotypes within the Lyssavirus genus of the Rhabdoviridae family, is believed to be exclusive to the African continent, where infections in various mammal species have been reported. After an isolation of Mokola virus at Umhlanga on the east coast of South Africa in 1970, the virus was no...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1008120511752

    authors: Nel L,Jacobs J,Jaftha J,von Teichman B,Bingham J,Olivier M

    更新日期:2000-01-01 00:00:00

  • Genetic mechanisms of antigenic variation in infectious bursal disease virus: analysis of a naturally occurring variant virus.

    abstract::The major immunogenic protein VP2 from a pathogenic field isolate (variant A virus) of infectious bursal disease virus (IBDV) was cloned and sequenced to examine antigenic variations. The VP2 open reading frame consists of 1509 nucleotides and codes for a 503 amino acid protein. Overall, the VP2 amino acid sequence of...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF01702563

    authors: Lana DP,Beisel CE,Silva RF

    更新日期:1992-08-01 00:00:00

  • Gene A32 product of vaccinia virus may be an ATPase involved in viral DNA packaging as indicated by sequence comparisons with other putative viral ATPases.

    abstract::Statistically significant sequence similarity was revealed between the gene A32 product of vaccinia virus (VV), gene I products (gpI) of filamentous single-stranded DNA bacteriophages, and IVa2 gene products of adenoviruses. Four conserved sequence motifs were delineated, the two N-proximal of which correspond to the ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF01702351

    authors: Koonin EV,Senkevich TG,Chernos VI

    更新日期:1993-02-01 00:00:00

  • Sequence of rice hoja blanca tenuivirus RNA-2.

    abstract::The sequence of rice hoja blanca tenuivirus RNA-2 is analysed and compared to its counter-part in rice stripe tenuivirus. The RNA encodes two proteins, in an ambisense arrangement. The 94 kD pc2, located in the complementary sense RNA, has several features typical of viral membrane (glyco)proteins, and also has region...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF00284643

    authors: De Miranda JR,Muñoz M,Wu R,Hull R,Espinoza AM

    更新日期:1996-01-01 00:00:00

  • A common structural core in the internal ribosome entry sites of picornavirus, hepatitis C virus, and pestivirus.

    abstract::Cap-independent translations of viral RNAs of enteroviruses and rhinoviruses, cardioviruses and aphthoviruses, hepatitis A and C viruses (HAV and HCV), and pestivirus are initiated by the direct binding of 40S ribosomal subunits to a cis-acting genetic element termed the internal ribosome entry site (IRES) or ribosome...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF00572952

    authors: Le SY,Siddiqui A,Maizel JV Jr

    更新日期:1996-01-01 00:00:00

  • The selection pressure analysis of chicken anemia virus structural protein gene VP1.

    abstract::Chicken anemia virus (CAV) is the pathogen of chicken infectious anemia. To clarify the driving force in CAV evolution, we have detected positive selection in the structural protein gene VP1 by using maximum-likelihood models. Strong evidence was found that VP1 proteins were subject to the high rates of positive selec...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-008-0316-z

    authors: Wang D,Fan W,Han GZ,He CQ

    更新日期:2009-04-01 00:00:00

  • Glycoprotein K of herpes simplex virus: a transmembrane protein encoded by the UL53 gene which regulates membrane fusion.

    abstract::Glycoprotein K (gK) encoded by the UL53 gene is the ninth out of eleven HSV glycoproteins (gps). The precursor gK (pgK) is a transmembrane protein with four hydrophobic domains, which consists of 338 amino acids. The UL53 gene has two initiation codons: the upper overlaps with the UL52 ORF, while the lower is located ...

    journal_title:Virus genes

    pub_type: 杂志文章,评审

    doi:10.1023/a:1008025520655

    authors: Rajcáni J,Kúdelová M

    更新日期:1999-01-01 00:00:00

  • Phylodynamic analysis of two amino acid substitutions in the hemagglutinin protein of canine distemper virus strains detected in fur-bearing animals in China.

    abstract::Canine distemper virus (CDV) causes a highly contagious disease in a wide range of carnivores. The hemagglutinin (H) protein of viruses shows the highest variability and plays an important role in modulation of viral antigenicity, virulence, and receptor recognition. Since 2012, canine distemper (CD) outbreaks in fur-...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-019-01720-9

    authors: Tao R,Ba H,Chen J,Liu M,Pan H,Li X,Wang Q,Zhao J

    更新日期:2020-02-01 00:00:00

  • A synthetic peptide derived from domain III envelope glycoprotein of Dengue virus induces neutralizing antibody.

    abstract::Dengue virus (DENV) is an arthropod-borne human pathogen that represents a severe public health threat in both endemic and non-endemic regions. So far, there is no licensed vaccine or specific drugs available for dengue fever. A fifteen-amino-acid-long peptide that includes the NGR motif was chemically synthesized and...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-017-1508-1

    authors: Mary JA,Jittmittraphap A,Chattanadee S,Leaungwutiwong P,Shenbagarathai R

    更新日期:2018-02-01 00:00:00

  • Genetic diversity of Apodemus agrarius-borne hantaan virus in Korea.

    abstract::Hantaan (HTN) virus, the etiologic agent of clinically severe hemorrhagic fever with renal syndrome (HFRS), was first isolated in 1976 from lung tissues of striped field mice (Apodemus agrarius) captured in Songnae-ri, Kyungki-do, Korea. To clarify the genetic diversity and phylogenetic relationship among Korean strai...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1008199800011

    authors: Song JW,Baek LJ,Kim SH,Kho EY,Kim JH,Yanagihara R,Song KJ

    更新日期:2000-10-01 00:00:00

  • Natural insertions within the N-terminal region of the coat protein of Maize dwarf mosaic potyvirus (MDMV) have an effect on the RNA stability.

    abstract::A 13 amino acid residue insertion was found in the N-terminal region of the coat protein of several Maize dwarf mosaic virus isolates (MDMV). These insertions seem to be the result of a direct duplication event, but differ in some positions. In order to evaluate the influence of the insertion on the RNA secondary stru...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-009-0425-3

    authors: Petrik K,Sebestyén E,Gell G,Balázs E

    更新日期:2010-02-01 00:00:00

  • Nucleotide sequence of the genome segment encoding nonstructural protein NS1 of bluetongue virus serotype 20 from Australia.

    abstract::The nucleotide sequence of the genome segment (S6) encoding the nonstructural protein NS1 of an Australian isolate of bluetongue virus serotype 20 (BTV 20) has been determined from a series of overlapping cDNA clones synthesized using two terminal 15-mer oligonucleotides as primers. The gene consists of 1769 nucleotid...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF01703087

    authors: Cowley JA

    更新日期:1992-11-01 00:00:00

  • A human vaccine strain of lamb rotavirus (Chinese) NSP4 gene: complete nucleotide sequence and phylogenetic analyses.

    abstract::A lamb strain of rotavirus has recently been licensed for use in China as a live vaccine to prevent rotavirus diarrhea in children. As rotavirus NSP4, especially the cytotoxic domain alone is considered to be associated with diarrhea, we sequenced gene segment 10, which encodes NSP4, of lamb rotavirus. Comparative ana...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1023491514820

    authors: Mohan KV,Kulkarni S,Glass RI,Zhisheng B,Atreya CD

    更新日期:2003-01-01 00:00:00

  • Cloning and characterization of a totivirus double-stranded RNA from the plant pathogenic fungus, Helicobasidium mompa Tanaka.

    abstract::Virus-like particles (VLPs, named HmTV1-17), about 40 nm in diameter were found in the violet root rot fungus Helicobasidium mompa Tanaka strain No. 17, which had been isolated from an apple tree. Purified preparations of HmTV1-17 contained two species of double-stranded RNA (dsRNA), designated 17L and 17S. cDNAs were...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1024453111809

    authors: Nomura K,Osaki H,Iwanami T,Matsumoto N,Ohtsu Y

    更新日期:2003-05-01 00:00:00

  • Loss of linkage disequilibrium and accelerated protein divergence in duplicated cytomegalovirus chemokine genes.

    abstract::Human CMV (hCMV) encodes several captured chemokine ligand and chemokine receptor genes that may play a role in immune evasion. The adjacent viral alpha-chemokine genes UL146 and UL147 appear to have duplicated subsequent to a recent gene capture event. Sequence data from multiple hCMV isolates suggest accelerated pro...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-005-2201-3

    authors: Arav-Boger R,Zong JC,Foster CB

    更新日期:2005-08-01 00:00:00

  • Sequence of Echinochloa hoja blanca tenuivirus RNA-4.

    abstract::The sequence is presented of RNA-4 of Echinochloa hoja blanca tenuivirus (EHBV), one of two tenuiviruses associated with rice cultivation in Latin America (together with rice hoja blanca virus; RHBV). Analysis of the sequence shows that the coding regions of EHBV RNA-4 are closely related to those of RHBV RNA-4. Howev...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF00576979

    authors: de Miranda JR,Muñoz M,Wu R,Espinoza AM

    更新日期:1996-01-01 00:00:00

  • Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences.

    abstract::Several important biological processes are performed by distinct functional domains found on replication-associated protein (Rep) encoded by AC1 of geminiviruses. Two truncated forms of replicase (tAC1) gene, capable of expressing only the N-terminal 669 bp (5'AC1) and C-terminal 783 bp (3'AC1) nucleotides cloned unde...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-011-0569-9

    authors: Hashmi JA,Zafar Y,Arshad M,Mansoor S,Asad S

    更新日期:2011-04-01 00:00:00

  • Evolution of surface and nonstructural-1 genes of influenza B viruses isolated in the Province of Québec, Canada, during the 1998-2001 period.

    abstract::After 2 minor winter seasons, influenza B viruses were predominantly isolated in the Province of Quebec, Canada, during the 2000-2001 season representing 74% of laboratory-confirmed influenza viruses. We performed an antigenic study of the hemagglutinin (HA) protein and a molecular characterization of the HA1 region, ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1025768308631

    authors: Abed Y,Coulthart MB,Li Y,Boivin G

    更新日期:2003-10-01 00:00:00

  • Phylogenetic analysis of the bovine parainfluenza virus type 3 from cattle herds revealing the existence of a genotype A strain in China.

    abstract::In 2009, a bovine parainfluenza virus (BPIV3), named as NM09, was isolated using MDBK cell culture from the nasal swabs of normal cattle in China. The NM09 isolate was characterized by RT-PCR and nucleotide sequence analysis. Its complete genome was 15,456 nucleotides in length. Similar to other sequenced PIV strains,...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-012-0810-1

    authors: Wen YJ,Shi XC,Wang FX,Wang W,Zhang SQ,Li G,Song N,Chen LZ,Cheng SP,Wu H

    更新日期:2012-12-01 00:00:00

  • A bioinformatics pipeline to search functional motifs within whole-proteome data: a case study of poxviruses.

    abstract::Proteins harbor domains or short linear motifs, which facilitate their functions and interactions. Finding functional motifs in protein sequences could predict the putative cellular roles or characteristics of hypothetical proteins. In this study, we present Shetti-Motif, which is an interactive tool to (i) map UniPro...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-016-1416-9

    authors: Sobhy H

    更新日期:2017-04-01 00:00:00

  • Antigenic variation in Foot and Mouth Disease Virus type Asia 1 isolates circulated during 1993-95 in India.

    abstract::The antigenic variation in Foot and Mouth Disease Virus (FMDV) is very high. The effective strategy to control the Foot and Mouth Disease (FMD) in India which is a habitat of four serotypes O, A, C and Asia 1, is by regular vaccination, using the vaccine strain most suitable for the local situation. India is an endemi...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1007984621839

    authors: Tulasiram P,Tyagi M,Srinivas K,Prabhudas K,Natarajan C,Suryanarayana V

    更新日期:1997-01-01 00:00:00

  • Identification of miR-221 and -222 as important regulators in genotype IV swine hepatitis E virus ORF3-expressing HEK 293 cells.

    abstract::Hepatitis E virus (HEV) has emerged as an important cause of epidemic and sporadic acute viral hepatitis worldwide, which is a major public health challenge. A better understanding of the interaction between the virus and the host cell would be very helpful for its therapy. Swine HEV (SHEV) open reading frame 3 (ORF3)...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-0912-4

    authors: Cheng Y,Du L,Shi Q,Jiao H,Zhang X,Hao Y,Rong H,Zhang J,Jia X,Guo S,Kuang W,Zhang H,Chen C,Wang F

    更新日期:2013-08-01 00:00:00

  • The genome of Beet cryptic virus 1 shows high homology to certain cryptoviruses present in phylogenetically distant hosts.

    abstract:UNLABELLED:This study determined the complete nucleotide sequence of Beet cryptic virus 1 (BCV1). As expected by analogy to previously sequenced alphacryptoviruses, dsRNA1 (2008 bp) encodes a 72.5-kDa protein containing sequence motifs characteristic for RNA-dependent RNA polymerases (RdRp). In addition to the full-len...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-009-0432-4

    authors: Szego A,Enünlü N,Deshmukh SD,Veliceasa D,Hunyadi-Gulyás E,Kühne T,Ilyés P,Potyondi L,Medzihradszky K,Lukács N

    更新日期:2010-04-01 00:00:00

  • Transcriptional profiling of Marek's disease virus genes during cytolytic and latent infection.

    abstract::Marek's disease (MD), a lymphoproliferative disease of chicken is caused by a highly cell-associated alpha-herpesvirus, Marek's disease virus (MDV). MDV replicates in chicken lymphocytes and establishes a latent infection within CD4(+) T cells. The expression analysis of limited viral transcripts have revealed differe...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-008-0203-7

    authors: Heidari M,Huebner M,Kireev D,Silva RF

    更新日期:2008-04-01 00:00:00