Abstract:
:The nucleotide sequence of the genome segment (S6) encoding the nonstructural protein NS1 of an Australian isolate of bluetongue virus serotype 20 (BTV 20) has been determined from a series of overlapping cDNA clones synthesized using two terminal 15-mer oligonucleotides as primers. The gene consists of 1769 nucleotides with an open reading frame between nucleotides 35 and 1690 encoding a protein of 552 amino acids (molecular weight 64,506 Da; net charge -2 at pH 7). Comparison of the nucleotide and deduced amino acid sequence of this genome segment with cognate segments of isolates of BTV 1 from Australia and South Africa, and BTV 10 and BTV 17 from the United States, revealed homologies of 98%, 80%, 79%, and 79%, respectively, at the nucleotide level and 98%, 90%, 89%, and 90% identity, respectively, at the amino acid level. The data indicate that the evolutionary divergence between NS1 genes of two different Australian BTV serotypes (BTV 20 and BTV 1) is less than that between isolates of the same (BTV 1) or different serotypes from different geographical locations.
journal_name
Virus Genesjournal_title
Virus genesauthors
Cowley JAdoi
10.1007/BF01703087subject
Has Abstractpub_date
1992-11-01 00:00:00pages
387-92issue
4eissn
0920-8569issn
1572-994Xjournal_volume
6pub_type
杂志文章相关文献
VIRUS GENES文献大全abstract::Pseudorabies virus (PRV) belongs to the Alphaherpesvirinae subfamily of Herpesviridae. PRV-induced pseudorabies is a highly contagious disease that has caused huge economic losses to the global swine industry. The PRV gE/gI gene deletion vaccine strain (Fa ΔgE/gI strain) constructed from the PRV Fa wild-type strain wa...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-020-01760-6
更新日期:2020-08-01 00:00:00
abstract::To determine the prevalence of TT virus (TTV) and GB virus C/hepatitis G virus (GBV-C) infections among patients with liver disease and the general population in Shanghai, China, we studied 90 patients with liver diseases (acute hepatitis, 28; chronic hepatitis, 27; liver cirrhosis, 20; hepatocellular carcinoma, 15) a...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008188623062
更新日期:1999-01-01 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV) causes a devastating enteric disease with acute diarrhea, dehydration and significant mortality in swine, thereby incurring heavy economic losses in Korea. Spike (S) glycoprotein has been suggested as an important determinant for PEDV biological properties. In this study, the nuc...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-007-0096-x
更新日期:2007-10-01 00:00:00
abstract::Protein 2A is a non-structural protein of coxsackievirus B3 (CVB3), an important human pathogen that can cause a variety of human diseases. Protein 2A not only participates in viral life cycle, but also regulates host cell functions; however, the underlying mechanisms remain poorly understood. In order to better under...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-015-1270-1
更新日期:2016-04-01 00:00:00
abstract::The incidence of watermelon chlorotic stunt disease and the molecular characterization of the Jordanian isolate of Watermelon chlorotic stunt virus (WmCSV-[JO]) are described in this study. Symptomatic leaf samples obtained from watermelon (Citrullus lanatus Thunb.), melon (Cucumis melo L.), squash (Cucurbita pepo), c...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-011-0594-8
更新日期:2011-08-01 00:00:00
abstract:UNLABELLED:This study determined the complete nucleotide sequence of Beet cryptic virus 1 (BCV1). As expected by analogy to previously sequenced alphacryptoviruses, dsRNA1 (2008 bp) encodes a 72.5-kDa protein containing sequence motifs characteristic for RNA-dependent RNA polymerases (RdRp). In addition to the full-len...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0432-4
更新日期:2010-04-01 00:00:00
abstract::Typically associated with fungal species, members of the viral family Totiviridae have recently been shown to be associated with plants, including important crop species, such as Carica papaya (papaya) and Zea mays (maize). Maize-associated totivirus (MATV) was first described in China and more recently in Ecuador, wh...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-019-01650-6
更新日期:2019-06-01 00:00:00
abstract::The nucleic acid and deduced amino acid sequence of the fibre gene of the HNF-61 strain of porcine adenovirus serotype 5 (PAdV-5) was determined and compared to that of the HNF-70 strain of the same serotype (Nagy et al., J Gen Virol 82, 525-529, 2001) and also to adenovirus fibre genes from the genera Mastadenovirus ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1014580802250
更新日期:2002-03-01 00:00:00
abstract::Endogenous retrovirus-like sequences that are homologous to the multigenic murine and Syrian hamster intracisternal type A particle (IAP) genes can be detected in very few copies in the Chinese hamster (CH) genome. They were cloned from a CHO gene library and two recombinants, harboring the major IAP-like DNA genes ch...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00570029
更新日期:1990-12-01 00:00:00
abstract::Porcine stool-associated RNA virus 1 (Posavirus 1) is a novel member of picornaviruses and first identified from fecal samples of 30-day-old pigs with diarrhea in USA in 2011. To evaluate the existence of Posavirus 1 in swine herds, 118 clinical samples from diarrheal pigs and 31 fecal swabs from healthy pigs were col...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-017-1512-5
更新日期:2018-02-01 00:00:00
abstract::This study brings the analysis of amino acid sequences of hemagglutinin (HA) from the influenza virus A that can infect a wide variety of birds and mammals. 191 sequences belonging to all known 15 HA subtypes were compared. The emphasis was given on functional sites (receptor-binding cavity with its right and left edg...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1014038120979
更新日期:2002-01-01 00:00:00
abstract::During October 2014, unfamiliar mild mosaic and mottling symptoms were identified on leaves of pepper (Capsicum chinense cv. Habanero) seedlings grown in the Arava valley in Israel 2-3 weeks post planting. Symptomatic plants were tested positive by ELISA using laboratory-produced antisera for tobamovirus species. Typi...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-018-1539-2
更新日期:2018-04-01 00:00:00
abstract::A goose parvovirus (GPV) Y strain was isolated from Muscovy ducks in Anhui Province of China. By polymerase chain reaction method, its complete genomic sequence was found to be 5,106 bp in length, consisting of 444-bp inverted terminal repeat, 1,844-bp non-structural protein and 2,199-bp capsid protein (VP) regions. T...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-1001-4
更新日期:2014-02-01 00:00:00
abstract::We describe the isolation and characterization of an insect-specific flavivirus (ISF) from Ochlerotatus caspius (Pallas, 1771) mosquitoes collected in southern Portugal. The RNA genome of this virus, tentatively designated OCFVPT, for O. caspius flavivirus from Portugal, encodes a polyprotein showing all the features ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-0960-9
更新日期:2013-12-01 00:00:00
abstract::Viral RNA from purified preparations of bean mild mosaic virus (BMMV) was resolved into two RNAs of mol. wt 1.5 X 10(6) daltons (R1) and 0.76 X 10(6) daltons (R2). Complementary DNA hybridizations, infectivity tests, and in-vitro translation studies established that R2 is a subgenomic RNA derived from R1. In-vitro tra...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00125339
更新日期:1989-05-01 00:00:00
abstract::Emerging pseudorabies virus (PRV) variant has led to frequent outbreaks of PRV infection among Bartha-K61-vaccinated swine population in Chinese swine farms and caused high mortality in pigs of all age since late 2011. Here, we generated a gE/gI-deleted PRV (rPRVXJ-delgI/gE-EGFP) based on PRV variant strain (PRV-XJ) t...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-017-1454-y
更新日期:2017-08-01 00:00:00
abstract::Caliciviruses are nonenveloped with a polyadenylated genome of approximately 7.6 kb and a single capsid protein. The "RNA Fold" computer program was used to analyze 3'-terminal noncoding sequences of five feline calicivirus (FCV), rabbit hemorrhagic disease virus (RHDV), and two San Miguel sea lion virus (SMSV) isolat...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF01704518
更新日期:1994-07-01 00:00:00
abstract::Although it is known that Cambodia is one of the high endemic area of hepatitis B virus (HBV) infection, molecular characterization of HBV circulating in this country has not been reported. In this study, pre-S gene of HBV from 12 Cambodian patients was sequenced. Phylogenetic analysis based on the pre-S gene sequence...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-008-0205-5
更新日期:2008-04-01 00:00:00
abstract::The exo-xis region of lambdoid phages contains open reading frames and genes that appear to be evolutionarily important. However, this region has received little attention up to now. In this study, we provided evidence that ea22, the largest gene of this region, favors the lysogenic pathway over the lytic pathway in c...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-020-01734-8
更新日期:2020-04-01 00:00:00
abstract::The 5(-terminal leader sequence of the equine arteritis virus (EAV) genome contains an open reading frame (ORF) with an AUG codon in a suboptimal context for initiation of protein synthesis. To investigate the significance of this intraleader ORF (ILO), an expression plasmid was generated carrying a DNA copy of the su...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-005-0030-z
更新日期:2006-08-01 00:00:00
abstract::Straw-colored fruit bats (Eidolon helvum), which have been identified as natural hosts for several zoonotic pathogens, such as lyssaviruses, henipaviruses, and ebolavirus, are associated with human settlements in Nigeria where they are commonly consumed as a delicacy. However, information on the viruses harbored by th...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1331-0
更新日期:2016-08-01 00:00:00
abstract::We report here nucleotide sequence and characterization of gene 11 from two tissue culture-adapted ATCC rhesus (RRV) and human (Wa) strains of rotavirus. Gene 11 sequence encodes a nonstructural protein, NSP5 and also encodes NSP6, from an out of phase open reading frame. Sequence of RRV(ATCC) gene 11 represents the f...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1012577407824
更新日期:2001-12-01 00:00:00
abstract::The antigenic variation in Foot and Mouth Disease Virus (FMDV) is very high. The effective strategy to control the Foot and Mouth Disease (FMD) in India which is a habitat of four serotypes O, A, C and Asia 1, is by regular vaccination, using the vaccine strain most suitable for the local situation. India is an endemi...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1007984621839
更新日期:1997-01-01 00:00:00
abstract::The complete genome sequence of the KGH strain of the first human rabies virus, which was isolated from a skin biopsy of a patient with rabies, whose symptoms developed due to bites from a raccoon dog in 2001. The size of the KGH strain genome was determined to be 11,928 nucleotides (nt) with a leader sequence of 58 n...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-012-0850-6
更新日期:2013-04-01 00:00:00
abstract::Complementary DNAs (cDNAs) to the RNA genome of a necrotic strain of potato virus Y in Japan (Hokkaido Univ. isolate of PVY-T:PVY-TH) were synthesized and cloned into a plasmid pBR322. About 4.3 kbp of the cDNA sequence containing the 3'-poly(A) tract of PVY-TH was inserted into a recombinant plasmid pBRYT88. The coat...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00570028
更新日期:1990-12-01 00:00:00
abstract::The California 99 (Cal99) variant of infectious bronchitis virus (IBV) was first recovered in 1999 from vaccinated broiler chicken flocks in Central California. The S1 hypervariable region of Cal99 genome was most closely related to Arkansas (Ark) serotype viruses. In this study, the complete genome of Cal99 was seque...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-006-0014-7
更新日期:2007-06-01 00:00:00
abstract::Human T-cell leukemia virus type 1 (HTLV-1) is a causative agent of adult T-cell leukemia. HTLV-1 Tax1 transforming protein interacts with several PDZ domain-containing proteins, and the interaction is associated with the transforming activities of Tax1 as well as persistent HTLV-1 infection. In this study, we show th...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-008-0259-4
更新日期:2008-10-01 00:00:00
abstract::We have described three mutant strains of Pseudorabies virus that contain mutations in the signal sequence coding region of a nonessential envelope glycoprotein, gIII. The alterations disrupt, truncate, or eliminate the hydrophobic core domain of the signal sequence. Each mutant was assayed for its ability to promote ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF01702345
更新日期:1993-02-01 00:00:00
abstract::Hepatitis B virus (HBV), a major causative agent of hepatocellular carcinoma (HCC), encodes an oncogenic X protein (HBx) that transcriptionally activates multiple viral and cellular promoters. The present study aimed to identify the specific gene mutation related to the clinical outcome of HCC. Seventy-two HBV-infecte...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-010-0557-5
更新日期:2011-04-01 00:00:00
abstract::Two double-stranded RNA (dsRNA) segments of a virus with a bipartite genome identified in fig (Ficus carica L.) and denoted Fig cryptic virus (FCV) were cloned and sequenced. Viral dsRNAs are 1696 bp (RNA-1) and 1415 bp (RNA-2) in size. RNA-1 contains a single ORF (1419 nt) potentially encoding a 54 kDa protein and co...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-011-0581-0
更新日期:2011-06-01 00:00:00