Abstract:
:The near-complete (99.7 %) genome sequence of a novel Russian Plum pox virus (PPV) isolate Pk, belonging to the strain Winona (W), has been determined by 454 pyrosequencing with the exception of the thirty-one 5'-terminal nucleotides. This region was amplified using 5'RACE kit and sequenced by the Sanger method. Genomic RNA released from immunocaptured PPV particles was employed for generation of cDNA library using TransPlex Whole transcriptome amplification kit (WTA2, Sigma-Aldrich). The entire Pk genome has identity level of 92.8-94.5 % when compared to the complete nucleotide sequences of other PPV-W isolates (W3174, LV-141pl, LV-145bt, and UKR 44189), confirming a high degree of variability within the PPV-W strain. The isolates Pk and LV-141pl are most closely related. The Pk has been found in a wild plum (Prunus domestica) in a new region of Russia indicating widespread dissemination of the PPV-W strain in the European part of the former USSR.
journal_name
Virus Genesjournal_title
Virus genesauthors
Sheveleva A,Kudryavtseva A,Speranskaya A,Belenikin M,Melnikova N,Chirkov Sdoi
10.1007/s11262-013-0946-7subject
Has Abstractpub_date
2013-10-01 00:00:00pages
385-8issue
2eissn
0920-8569issn
1572-994Xjournal_volume
47pub_type
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