Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences.


:Several important biological processes are performed by distinct functional domains found on replication-associated protein (Rep) encoded by AC1 of geminiviruses. Two truncated forms of replicase (tAC1) gene, capable of expressing only the N-terminal 669 bp (5'AC1) and C-terminal 783 bp (3'AC1) nucleotides cloned under transcriptional control of the CaMV35S were introduced into cotton (Gossypium hirsutum L.) using LBA4404 strain of Agrobacterium tumefaciens to make use of an interference strategy for impairing cotton leaf curl virus (CLCuV) infection in transgenic cotton. Compared with nontransformed control, we observed that transgenic cotton plants overexpressing either N-terminal (5'AC1) or C-terminal (3'AC1) sequences confer resistance to CLCuV by inhibiting replication of viral genomic and β satellite DNA components. Molecular analysis by Northern blot hybridization revealed high transgene expression in early and late growth stages associated with inhibition of CLCuV replication. Of the eight T(1) transgenic lines tested, six had delayed and minor symptoms as compared to nontransformed control lines which developed disease symptoms after 2-3 weeks of whitefly-mediated viral delivery. Virus biological assay and growth of T(2) plants proved that transgenic cotton plants overexpressing 5'- and 3'AC1 displayed high resistance level up to 72, 81%, respectively, as compared to non-transformed control plants following inoculation with viruliferous whiteflies giving significantly high cotton seed yield. Progeny analysis of these plants by polymerase chain reaction (PCR), Southern blotting and virus biological assay showed stable transgene, integration, inheritance and cotton leaf curl disease (CLCuD) resistance in two of the eight transgenic lines having single or two transgene insertions. Transgenic cotton expressing partial AC1 gene of CLCuV can be used as virus resistance source in cotton breeding programs aiming to improve virus resistance in cotton crop.


Virus Genes


Virus genes


Hashmi JA,Zafar Y,Arshad M,Mansoor S,Asad S




Has Abstract


2011-04-01 00:00:00












  • Molecular epidemiology of PRRSV from China's Guangxi Province between 2007 and 2009.

    abstract::Porcine reproductive and respiratory syndrome (PRRS) is considered to be one of the most important infectious diseases affecting livestock. This study used gene sequence analysis of ORF5 and Nsp2 to determine the molecular epidemiology of PRRSV in different parts of the Guangxi province of China. These genes were sele...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Zhang HY,Liang JJ,Meng XM,Li H,Yang J,Su LJ,Zhang HP,Xie LJ,He XX,Li YS,Yin S,Li XQ,Li XN,Luo TR

    更新日期:2013-02-01 00:00:00

  • Interactions of porcine circovirus 2 with its hosts.

    abstract::Porcine circovirus 2 (PCV2) can cause porcine circovirus diseases and porcine circovirus-associated diseases (PCVD/PCVAD), which are widely presented in swine-producing countries. Since the discovery of this virus, considerable efforts have been devoted to understanding this pathogen and its interactions with its host...

    journal_title:Virus genes

    pub_type: 杂志文章,评审


    authors: Ren L,Chen X,Ouyang H

    更新日期:2016-08-01 00:00:00

  • Diversity and distribution of Maize-associated totivirus strains from Tanzania.

    abstract::Typically associated with fungal species, members of the viral family Totiviridae have recently been shown to be associated with plants, including important crop species, such as Carica papaya (papaya) and Zea mays (maize). Maize-associated totivirus (MATV) was first described in China and more recently in Ecuador, wh...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Read DA,Featherston J,Rees DJG,Thompson GD,Roberts R,Flett BC,Mashingaidze K,Pietersen G,Kiula B,Kullaya A,Mbega ER

    更新日期:2019-06-01 00:00:00

  • Comparative full genome analysis of four infectious laryngotracheitis virus (Gallid herpesvirus-1) virulent isolates from the United States.

    abstract::Gallid herpesvirus-1 (GaHV-1), commonly named infectious laryngotracheitis (ILT) virus, causes the respiratory disease in chickens known as ILT. The molecular determinants associated with differences in pathogenicity of GaHV-1 strains are not completely understood, and a comparison of genomic sequences of isolates tha...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Spatz SJ,Volkening JD,Keeler CL,Kutish GF,Riblet SM,Boettger CM,Clark KF,Zsak L,Afonso CL,Mundt ES,Rock DL,Garcia M

    更新日期:2012-04-01 00:00:00

  • Base usage and dinucleotide frequency of infectious bursal disease virus.

    abstract::Base usage and dinucleotide frequency have been extensively studied in many eukaryotic organisms and bacteria, but not for viruses. In this paper, a comprehensive analysis of these aspects for infectious bursal disease virus (IBDV) was presented. The analysis of base usage indicated that all of the IBDV genes possess ...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Tan DY,Hair Bejo M,Aini I,Omar AR,Goh YM

    更新日期:2004-01-01 00:00:00

  • A local strain of Paprika mild mottle virus breaks L3 resistance in peppers and is accelerated in Tomato brown rugose fruit virus-infected Tm-22-resistant tomatoes.

    abstract::During October 2014, unfamiliar mild mosaic and mottling symptoms were identified on leaves of pepper (Capsicum chinense cv. Habanero) seedlings grown in the Arava valley in Israel 2-3 weeks post planting. Symptomatic plants were tested positive by ELISA using laboratory-produced antisera for tobamovirus species. Typi...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Luria N,Smith E,Sela N,Lachman O,Bekelman I,Koren A,Dombrovsky A

    更新日期:2018-04-01 00:00:00

  • Molecular analysis of the hexon, penton base, and fiber-2 genes of Korean fowl adenovirus serotype 4 isolates from hydropericardium syndrome-affected chickens.

    abstract::Fowl adenovirus serotype 4 (FAdV-4) is the causative agent of hydropericardium syndrome (HPS), a highly pathogenic disease in poultry. In the present study, hexon, penton base, and fiber-2 genes encoding major capsid proteins were analyzed in four FAdV-4 isolates from HPS-affected chickens in Korea. Nucleotide sequenc...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Park HS,Lim IS,Kim SK,Kim TK,Park CK,Yeo SG

    更新日期:2017-02-01 00:00:00

  • BK virus DNA cloned directly from human urine confirms an archetypal structure for the transcriptional control region.

    abstract::A BK virus isolate cloned from the urine of an immunosuppressed patient was identified as the WW strain. Fine mapping using four base-pair restriction enzymes identified a number of small deletions and insertions relative to prototype BK. The finding of a transcriptional control region with a structure similar to that...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Rubinstein R,Harley EH

    更新日期:1989-03-01 00:00:00

  • Expression of the X protein of hepatitis B virus in insect cells using recombinant baculoviruses.

    abstract::The baculovirus system was used to express the X protein of human hepatitis B virus (HBV). The X open reading frames (X ORFs) from cloned viral DNA of the HBV subtypes ayw and adr were introduced into the genome of Autographa californica nuclear polyhedrosis virus (AcNPV). The HBV-DNA of subtype adr derived from a hep...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Klein R,Schröder CH,Zentgraf H

    更新日期:1991-04-01 00:00:00

  • Insights into Jumonji C-domain containing protein 6 (JMJD6): a multifactorial role in foot-and-mouth disease virus replication in cells.

    abstract::The Jumonji C-domain containing protein 6 (JMJD6) has had a convoluted history, and recent reports indicating a multifactorial role in foot-and-mouth disease virus (FMDV) infection have further complicated the functionality of this protein. It was first identified as the phosphatidylserine receptor on the cell surface...

    journal_title:Virus genes

    pub_type: 杂志文章,评审


    authors: Lawrence P,Rieder E

    更新日期:2017-06-01 00:00:00

  • Characterization and complete genome sequence analysis of Staphylococcus aureus bacteriophage SA12.

    abstract::Staphylococcus aureus is a well-known pathogen that causes several serious diseases in humans and animals. As part of the efforts to control this pathogen, a newly isolated bacteriophage, SA12, which specifically targets S. aureus, was characterized, and its genome was completely sequenced. Host range and bacteriophag...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Chang Y,Lee JH,Shin H,Heu S,Ryu S

    更新日期:2013-10-01 00:00:00

  • Diversifying evolution of highly pathogenic H5N1 avian influenza virus in Egypt from 2006 to 2011.

    abstract::An evolutionary analysis was conducted of 354 hemagglutinin (HA) and 208 neuraminidase (NA) genes, including newly generated sequences of 5 HA and 30 NA, of Egyptian H5N1 clade 2.2.1 viruses isolated from poultry and humans. Five distinct phylogenetically distinguishable clusters arose from a monophyletic origin since...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Abdelwhab EM,Arafa AS,Stech J,Grund C,Stech O,Graeber-Gerberding M,Beer M,Hassan MK,Aly MM,Harder TC,Hafez HM

    更新日期:2012-08-01 00:00:00

  • Hamster polyomavirus-derived virus-like particles are able to transfer in vitro encapsidated plasmid DNA to mammalian cells.

    abstract::The authentic major capsid protein 1 (VP1) of hamster polyomavirus (HaPyV) consists of 384 amino acid (aa) residues (42 kDa). Expression from an additional in-frame initiation codon located upstream from the authentic VP1 open reading frame (at position -4) might result in the synthesis of a 388 aa-long, amino-termina...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Voronkova T,Kazaks A,Ose V,Ozel M,Scherneck S,Pumpens P,Ulrich R

    更新日期:2007-06-01 00:00:00

  • Rapid detection of highly pathogenic porcine reproductive and respiratory syndrome virus by a fluorescent probe-based isothermal recombinase polymerase amplification assay.

    abstract::A novel fluorescent probe-based real-time reverse transcription recombinase polymerase amplification (real-time RT-RPA) assay was developed for rapid detection of highly pathogenic type 2 porcine reproductive and respiratory syndrome virus (HP-PRRSV). The sensitivity analysis showed that the detection limit of RPA was...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Yang Y,Qin X,Sun Y,Chen T,Zhang Z

    更新日期:2016-12-01 00:00:00

  • Antigenic variation in Foot and Mouth Disease Virus type Asia 1 isolates circulated during 1993-95 in India.

    abstract::The antigenic variation in Foot and Mouth Disease Virus (FMDV) is very high. The effective strategy to control the Foot and Mouth Disease (FMD) in India which is a habitat of four serotypes O, A, C and Asia 1, is by regular vaccination, using the vaccine strain most suitable for the local situation. India is an endemi...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Tulasiram P,Tyagi M,Srinivas K,Prabhudas K,Natarajan C,Suryanarayana V

    更新日期:1997-01-01 00:00:00

  • The genome sequence of an H11N2 avian influenza virus from a Thick-billed Murre (Uria lomvia) shows marine-specific and regional patterns of relationships to other viruses.

    abstract::Influenza A viruses infect a range of host species, including various mammals and more than 100 species of birds. For avian influenza viruses (AIV), prevalence varies between different groups of birds, with waterfowl showing the highest prevalence. We have sequenced the complete genome of A/Thick-billed Murre/Newfound...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Granter A,Wille M,Whitney H,Robertson GJ,Ojkic D,Lang AS

    更新日期:2010-10-01 00:00:00

  • Sequence of rice hoja blanca tenuivirus RNA-2.

    abstract::The sequence of rice hoja blanca tenuivirus RNA-2 is analysed and compared to its counter-part in rice stripe tenuivirus. The RNA encodes two proteins, in an ambisense arrangement. The 94 kD pc2, located in the complementary sense RNA, has several features typical of viral membrane (glyco)proteins, and also has region...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: De Miranda JR,Muñoz M,Wu R,Hull R,Espinoza AM

    更新日期:1996-01-01 00:00:00

  • Creation of diversity in the animal virus world by inter-species and intra-species recombinations: lessons learned from poultry viruses.

    abstract::The biological diversity within viruses is one of the largest found in all other forms of nature. Many mechanisms contribute to virus diversity and include incorporating genetic material from the host, recombination between viruses belonging to the same or to a different family, and even recombination between viruses ...

    journal_title:Virus genes

    pub_type: 杂志文章,评审


    authors: Davidson I,Silva RF

    更新日期:2008-02-01 00:00:00

  • Interaction between Translocation-associated membrane protein 1 and σC protein of novel duck reovirus controls virus infectivity.

    abstract::Novel duck reovirus (NDRV), the prototype strain of the species Avian orthoreovirus (ARV), is associated with high mortality in Pekin ducklings. σC is an outer capsid protein encoded by the S1 genome segment of NDRV which mediates attachment to host cells. Our previous studies using immunoprecipitation and mass spectr...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Xiao R,Mi X,Sun J,Ding M,Li C,Zhu J,Liu G,Ma W,Zhou H,Chen Z

    更新日期:2020-06-01 00:00:00

  • A live gI/gE-deleted pseudorabies virus (PRV) protects weaned piglets against lethal variant PRV challenge.

    abstract::Emerging pseudorabies virus (PRV) variant has led to frequent outbreaks of PRV infection among Bartha-K61-vaccinated swine population in Chinese swine farms and caused high mortality in pigs of all age since late 2011. Here, we generated a gE/gI-deleted PRV (rPRVXJ-delgI/gE-EGFP) based on PRV variant strain (PRV-XJ) t...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Yin Y,Xu Z,Liu X,Li P,Yang F,Zhao J,Fan Y,Sun X,Zhu L

    更新日期:2017-08-01 00:00:00

  • Expression of VP2 gene protein of infectious bursal disease virus detected in Korea.

    abstract::The VP2 gene DNA (1.4 kb in approximate) of a very virulent infectious bursal disease virus (vvIBDV) Chinju strain detected in Chinju, Korea was cloned into the bacmid, a baculovirus shuttle vector, through transposition of the gene from initially cloned pFastBacHTa plasmid, a baculovirus expression vector, and was su...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Kim TK,Yeo SG

    更新日期:2003-08-01 00:00:00

  • Nucleotide sequencing and serologic analysis of Cache Valley virus isolates from the Yucatan Peninsula of Mexico.

    abstract::Nucleotide sequencing was performed on part of the medium and large genome segments of 17 Cache Valley virus (CVV) isolates from the Yucatan Peninsula of Mexico. Alignment of these sequences to all other sequences in the Genbank database revealed that they have greatest nucleotide identity (97-98 %) with the equivalen...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Blitvich BJ,Loroño-Pino MA,Garcia-Rejon JE,Farfan-Ale JA,Dorman KS

    更新日期:2012-08-01 00:00:00

  • Capsid protein gene variation among feline calicivirus isolates.

    abstract::We amplified the capsid protein gene fragments of 30 Japanese isolates of feline calicivirus (FCV), including the C, D, and E regions, by reverse transcription-polymerase chain reaction (RT-PCR), followed by direct sequencing. Alignment of the predicted amino acid sequences, together with other published sequences fro...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Horimoto T,Takeda Y,Iwatsuki-Horimoto K,Sugii S,Tajima T

    更新日期:2001-01-01 00:00:00

  • hycu-hr6, A large homologous region of the Hyphantria cunea nucleopolyhedrovirus genome, as a powerful and versatile enhancer in insect expression systems.

    abstract::We previously identified a large homologous region (hr), hycu-hr6, in the genome of the Hyphantria cunea nucleopolyhedrovirus (HycuNPV) and suggested that hycu-hr6 was the largest baculovirus promoter enhancer hr identified so far. In this study, we examined the enhancement activity of hycu-hr6 against two promoters f...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Felipe Alves CA,Ishikawa H,Ikeda M,Kobayashi M

    更新日期:2009-12-01 00:00:00

  • Identification of specific amino acid residues of adenovirus 12 E1A involved in transformation and p300 binding.

    abstract::The early region 1A (E1A) gene of highly oncogenic adenovirus type 12 (Ad12) was analyzed for transforming activity and protein binding using specific mutations. The Ad12 E1A proteins were found to bind p300 protein mainly within the CR1 region, although mutations that affect both p300 binding and transformation were ...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Sawada Y,Ishino M,Miura K,Ohtsuka E,Fujinaga K

    更新日期:1997-01-01 00:00:00

  • Genomic analysis of a recombinant NADC30-like porcine reproductive and respiratory syndrome virus in china.

    abstract::Recently, NADC30-like porcine reproductive and respiratory syndrome viruses (PRRSVs), which are genetically similar to the NADC30 strain isolated in the United States of America in 2008, have become prevalent in China. Here, a novel variant PRRSV strain named HNhx was successfully isolated on porcine alveolar macropha...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Wang LJ,Wan B,Guo Z,Qiao S,Li R,Xie S,Chen XX,Zhang G

    更新日期:2018-02-01 00:00:00

  • Complete nucleotide sequences and construction of full-length infectious cDNA clones of cucumber green mottle mosaic virus (CGMMV) in a versatile newly developed binary vector including both 35S and T7 promoters.

    abstract::Seed-transmitted viruses have caused significant damage to watermelon crops in Korea in recent years, with cucumber green mottle mosaic virus (CGMMV) infection widespread as a result of infected seed lots. To determine the likely origin of CGMMV infection, we collected CGMMV isolates from watermelon and melon fields a...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Park CH,Ju HK,Han JY,Park JS,Kim IH,Seo EY,Kim JK,Hammond J,Lim HS

    更新日期:2017-04-01 00:00:00

  • Analysis of a chitinase from EpapGV, a fast killing betabaculovirus.

    abstract::The main function of baculoviral chitinase protein (V-CHIA) is to promote the final liquefaction of infected host larvae, facilitating the dispersion of occlusion bodies (OBs) in the environment. In this study, a v-chiA from Epinotia aporema Granulovirus (EpapGV) was identified and characterized. The 1,713 base pairs ...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Salvador R,Ferrelli ML,Sciocco-Cap A,Romanowski V

    更新日期:2014-04-01 00:00:00

  • Molecular characterization of the full-length genome of a rabies virus isolate from India.

    abstract::Rabies is an important public health problem in South East Asia, with cases in this part of the world contributing to about 70% of the global burden. A large number of rabies cases occur in India, however, there is no organized system of surveillance and hence there is a lack of reliable data. Moreover, comprehensive ...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Nagaraja T,Madhusudana S,Desai A

    更新日期:2008-06-01 00:00:00

  • Variation analysis of two cucumber mosaic viruses and their associated satellite RNAs from sugar beet in China.

    abstract::Two cucumber mosaic virus (CMV) isolates XJ1 and XJ2 were obtained from sugar beet showing yellow mosaic symptom in Shihezi, Xinjiang Uigur municipality of China. The coat protein gene of the two CMV isolates and their associated satellite RNAs were amplified by reverse transcriptase polymerase chain reaction (RT-PCR)...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Xi D,Lan L,Wang J,Xu W,Xiang B,Lin H

    更新日期:2006-12-01 00:00:00