Engineering cotton (Gossypium hirsutum L.) for resistance to cotton leaf curl disease using viral truncated AC1 DNA sequences.


:Several important biological processes are performed by distinct functional domains found on replication-associated protein (Rep) encoded by AC1 of geminiviruses. Two truncated forms of replicase (tAC1) gene, capable of expressing only the N-terminal 669 bp (5'AC1) and C-terminal 783 bp (3'AC1) nucleotides cloned under transcriptional control of the CaMV35S were introduced into cotton (Gossypium hirsutum L.) using LBA4404 strain of Agrobacterium tumefaciens to make use of an interference strategy for impairing cotton leaf curl virus (CLCuV) infection in transgenic cotton. Compared with nontransformed control, we observed that transgenic cotton plants overexpressing either N-terminal (5'AC1) or C-terminal (3'AC1) sequences confer resistance to CLCuV by inhibiting replication of viral genomic and β satellite DNA components. Molecular analysis by Northern blot hybridization revealed high transgene expression in early and late growth stages associated with inhibition of CLCuV replication. Of the eight T(1) transgenic lines tested, six had delayed and minor symptoms as compared to nontransformed control lines which developed disease symptoms after 2-3 weeks of whitefly-mediated viral delivery. Virus biological assay and growth of T(2) plants proved that transgenic cotton plants overexpressing 5'- and 3'AC1 displayed high resistance level up to 72, 81%, respectively, as compared to non-transformed control plants following inoculation with viruliferous whiteflies giving significantly high cotton seed yield. Progeny analysis of these plants by polymerase chain reaction (PCR), Southern blotting and virus biological assay showed stable transgene, integration, inheritance and cotton leaf curl disease (CLCuD) resistance in two of the eight transgenic lines having single or two transgene insertions. Transgenic cotton expressing partial AC1 gene of CLCuV can be used as virus resistance source in cotton breeding programs aiming to improve virus resistance in cotton crop.


Virus Genes


Virus genes


Hashmi JA,Zafar Y,Arshad M,Mansoor S,Asad S




Has Abstract


2011-04-01 00:00:00












  • Characterization and complete genome sequence analysis of Staphylococcus aureus bacteriophage SA12.

    abstract::Staphylococcus aureus is a well-known pathogen that causes several serious diseases in humans and animals. As part of the efforts to control this pathogen, a newly isolated bacteriophage, SA12, which specifically targets S. aureus, was characterized, and its genome was completely sequenced. Host range and bacteriophag...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Chang Y,Lee JH,Shin H,Heu S,Ryu S

    更新日期:2013-10-01 00:00:00

  • First complete genome sequences of genogroup V, genotype 3 porcine sapoviruses: common 5'-terminal genomic feature of sapoviruses.

    abstract::Sapoviruses (SaVs) are enteric viruses and have been detected in various mammals. They are divided into multiple genogroups and genotypes based on the entire major capsid protein (VP1) encoding region sequences. In this study, we determined the first complete genome sequences of two genogroup V, genotype 3 (GV.3) SaV ...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Oka T,Doan YH,Shimoike T,Haga K,Takizawa T

    更新日期:2017-12-01 00:00:00

  • The ea22 gene of lambdoid phages: preserved prolysogenic function despite of high sequence diversity.

    abstract::The exo-xis region of lambdoid phages contains open reading frames and genes that appear to be evolutionarily important. However, this region has received little attention up to now. In this study, we provided evidence that ea22, the largest gene of this region, favors the lysogenic pathway over the lytic pathway in c...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Dydecka A,Bloch S,Necel A,Topka G,Węgrzyn A,Tong J,Donaldson LW,Węgrzyn G,Nejman-Faleńczyk B

    更新日期:2020-04-01 00:00:00

  • Characterization of white spot syndrome virus VP52B and its interaction with VP26.

    abstract::White spot syndrome virus (WSSV) is one of the major pathogens of cultured shrimp. Identification of envelope protein interactions has become a central issue for the understanding of WSSV assembly. In this paper, WSSV envelope protein VP52B was fused with GST-tag and expressed in Escherichia coli BL-21(DE3). Immunogol...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Lin F,Jie Z,Hou L,Li F,Yang F

    更新日期:2015-02-01 00:00:00

  • A new potyvirus first isolated and identified from Angelica sinensis.

    abstract::A filamentous virus was isolated in Angelica sinensis (Angelica sinensis (Oliv.) Diels) which shows mosaic symptoms on leaves in Minxian, Gansu province, China. According to morphology and molecular biology properties, this virus, which has a flexuous rod-shaped particle about 750 nm in length and 12 nm in width, was ...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Zhang Y,Wang R,Wang J,Chang J,Zhang X,Chen T,An L,Xu S

    更新日期:2009-08-01 00:00:00

  • Correction to: Molecular analysis of maize (Zea mays L.)-infecting mastreviruses in Ethiopia reveals marked diversity of virus genomes and a novel species.

    abstract::The original version of this article unfortunately contained errors in the "Results" section. ...

    journal_title:Virus genes

    pub_type: 已发布勘误


    authors: Guadie D,Tesfaye K,Knierim D,Winter S,Abraham A

    更新日期:2019-06-01 00:00:00

  • Genomic analysis of an effective lentiviral vaccine-attenuated equine infectious anemia virus vaccine EIAV FDDV13.

    abstract::Chinese equine infectious anemia virus (EIAV) attenuated vaccine is the first lentiviral vaccine with a successful application. In order to understand the correlation of viral genomic mutations with viral attenuation and with induced immunoprotective properties, we analyzed the proviral genome sequences of the EIAV-at...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Qi X,Wang X,Wang S,Lin Y,Jiang C,Ma J,Zhao L,Lv X,Shen R,Wang F,Kong X,Su Z,Zhou J

    更新日期:2010-08-01 00:00:00

  • Detection by next generation sequencing of a multi-segmented viral genome from sugarcane associated with Ramu stunt disease.

    abstract::Ramu stunt disease of sugarcane was first reported in Papua New Guinea in the mid 1980s. The disease can reduce sugarcane yields significantly and causes severe stunting and mortality in highly susceptible cultivars. The causal agent of Ramu stunt has been investigated but its characterization has not been completed. ...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Mollov D,Maroon-Lango C,Kuniata L

    更新日期:2016-02-01 00:00:00

  • Identification of a thymidylate synthase gene within the genome of Chilo iridescent virus.

    abstract::The thymidylate synthase (TS, EC is essential for the de novo synthesis of dTMP in pro- and eucaryotic organisms. Consequently it plays a major role in the replication of the DNA genome of a cell or a DNA virus. The gene encoding the TS of Chilo iridescent virus (CIV) was identified by nucleotide sequence an...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Müller K,Tidona CA,Bahr U,Darai G

    更新日期:1998-01-01 00:00:00

  • Herpesvirus homologues of cellular genes.

    abstract::For millions of years viruses have adapted strategies to interfere with the immune defense of the host, which in turn has to deal with this challenge. In general the antiviral defense remains one step behind the pathogen. To achieve this strategic advantage large DNA-containing Viruses encode cellular homologues that ...

    journal_title:Virus genes

    pub_type: 杂志文章,评审


    authors: Raftery M,Müller A,Schönrich G

    更新日期:2000-01-01 00:00:00

  • The genome of Beet cryptic virus 1 shows high homology to certain cryptoviruses present in phylogenetically distant hosts.

    abstract:UNLABELLED:This study determined the complete nucleotide sequence of Beet cryptic virus 1 (BCV1). As expected by analogy to previously sequenced alphacryptoviruses, dsRNA1 (2008 bp) encodes a 72.5-kDa protein containing sequence motifs characteristic for RNA-dependent RNA polymerases (RdRp). In addition to the full-len...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Szego A,Enünlü N,Deshmukh SD,Veliceasa D,Hunyadi-Gulyás E,Kühne T,Ilyés P,Potyondi L,Medzihradszky K,Lukács N

    更新日期:2010-04-01 00:00:00

  • Inhibiting replication of begomoviruses using artificial zinc finger nucleases that target viral-conserved nucleotide motif.

    abstract::Geminiviridae consists of a large group of single-stranded DNA viruses that cause tremendous losses worldwide. Frequent mixed infection and high rates of recombination and mutation allow them to adapt rapidly to new hosts and overcome hosts' resistances. Therefore, an effective strategy able to confer plants with resi...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Chen W,Qian Y,Wu X,Sun Y,Wu X,Cheng X

    更新日期:2014-06-01 00:00:00

  • Parrot bornavirus-2 and -4 RNA detected in wild bird samples in Japan are phylogenetically adjacent to those found in pet birds in Japan.

    abstract::Bornaviruses (family Bornaviridae) are non-segmented negative-strand RNA viruses. Avian bornaviruses (ABVs), which are causative agents of proventricular dilatation disease, are a genetically diverse group with at least 15 genotypes, including parrot bornaviruses (PaBVs) and aquatic bird bornavirus 1(ABBV-1). Borna di...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Sassa Y,Bui VN,Saitoh K,Watanabe Y,Koyama S,Endoh D,Horie M,Tomonaga K,Furuya T,Nagai M,Omatsu T,Imai K,Ogawa H,Mizutani T

    更新日期:2015-10-01 00:00:00

  • Phylodynamic analysis of two amino acid substitutions in the hemagglutinin protein of canine distemper virus strains detected in fur-bearing animals in China.

    abstract::Canine distemper virus (CDV) causes a highly contagious disease in a wide range of carnivores. The hemagglutinin (H) protein of viruses shows the highest variability and plays an important role in modulation of viral antigenicity, virulence, and receptor recognition. Since 2012, canine distemper (CD) outbreaks in fur-...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Tao R,Ba H,Chen J,Liu M,Pan H,Li X,Wang Q,Zhao J

    更新日期:2020-02-01 00:00:00

  • Complete nucleotide sequence, genome organization and phylogenic analysis of the canine calicivirus.

    abstract::The complete genomic sequence of canine calicivirus (CaCV) isolated from feces of a dog with diarrhea was determined. The CaCV genome, a positive-sense single-stranded RNA, contained 8513 nucleotides excluding the poly(A) tail and was longer than that of any other calicivirus strain with a completely known sequence. T...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Matsuura Y,Tohya Y,Nakamura K,Shimojima M,Roerink F,Mochizuki M,Takase K,Akashi H,Sugimura T

    更新日期:2002-01-01 00:00:00

  • The genome sequence of an H11N2 avian influenza virus from a Thick-billed Murre (Uria lomvia) shows marine-specific and regional patterns of relationships to other viruses.

    abstract::Influenza A viruses infect a range of host species, including various mammals and more than 100 species of birds. For avian influenza viruses (AIV), prevalence varies between different groups of birds, with waterfowl showing the highest prevalence. We have sequenced the complete genome of A/Thick-billed Murre/Newfound...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Granter A,Wille M,Whitney H,Robertson GJ,Ojkic D,Lang AS

    更新日期:2010-10-01 00:00:00

  • Phylogenetic analysis of hop and grapevine isolates of hop stunt viroid supports a grapevine origin for hop stunt disease.

    abstract::We have examined sequence variability among nine isolates of hop stunt viroid (HSVd) collected from hop gardens in Tohoku district in Japan, the only area in the world where hop stunt disease is endemic. Six different consensus and one-consensus sequences as well as 12 sequence variants were detected in the nine HSVd-...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Sano T,Mimura R,Ohshima K

    更新日期:2001-01-01 00:00:00

  • Genotypic and phenotypic characterization of the California 99 (Cal99) variant of infectious bronchitis virus.

    abstract::The California 99 (Cal99) variant of infectious bronchitis virus (IBV) was first recovered in 1999 from vaccinated broiler chicken flocks in Central California. The S1 hypervariable region of Cal99 genome was most closely related to Arkansas (Ark) serotype viruses. In this study, the complete genome of Cal99 was seque...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Mondal SP,Cardona CJ

    更新日期:2007-06-01 00:00:00

  • Overall signal sequence hydrophobicity determines the in vivo translocation efficiency of a herpesvirus glycoprotein.

    abstract::We have described three mutant strains of Pseudorabies virus that contain mutations in the signal sequence coding region of a nonessential envelope glycoprotein, gIII. The alterations disrupt, truncate, or eliminate the hydrophobic core domain of the signal sequence. Each mutant was assayed for its ability to promote ...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Ryan P,Robbins A,Whealy M,Enquist LW

    更新日期:1993-02-01 00:00:00

  • Genomic characterization of a slow/low maedi visna virus.

    abstract::The complete genomic sequence of a sheep lentivirus isolate that presents a slow/low phenotype in vitro has been determined. The virus, designated P1OLV, was isolated from lung cells of a naturally infected sheep in Portugal. Three overlapping DNA fragments amplified by PCR, and encompassing the entire viral genome we...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Barros SC,Ramos F,Duarte M,Fagulha T,Cruz B,Fevereiro M

    更新日期:2004-10-01 00:00:00

  • Chicken skin virome analyzed by high-throughput sequencing shows a composition highly different from human skin.

    abstract::Recent studies show that human skin at homeostasis is a complex ecosystem whose virome include circular DNA viruses, especially papillomaviruses and polyomaviruses. To determine the chicken skin virome in comparison with human skin virome, a chicken swabs pool sample from fifteen indoor healthy chickens of five geneti...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Denesvre C,Dumarest M,Rémy S,Gourichon D,Eloit M

    更新日期:2015-10-01 00:00:00

  • Capsid protein diversity among Norwalk-like viruses.

    abstract::We have determined the complete capsid gene sequence of 20 Norwalk-like viruses (NLVs) collected predominantly from outbreaks in the UK between 1989 and 1996. These comprised nine genogroup I and eleven genogroup II strains. Phylogenetic analysis of these and 15 published sequences suggest seven genomic sub-groups wit...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Green J,Vinje J,Gallimore CI,Koopmans M,Hale A,Brown DW,Clegg JC,Chamberlain J

    更新日期:2000-01-01 00:00:00

  • Comparison of Schmallenberg virus sequences isolated from mammal host and arthropod vector.

    abstract::Schmallenberg virus (SBV) is the member of Peribunyaviridae family, which comprises pathogens of importance for human and veterinary medicine. The virus is transmitted only between animals and mainly by biting midges of the genus Culicoides. This study was performed in order to determine SBV genetic diversity and eluc...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Kęsik-Maliszewska J,Antos A,Rola J,Larska M

    更新日期:2018-12-01 00:00:00

  • The characteristics and genome analysis of vB_ApiP_XC38, a novel phage infecting Acinetobacter pittii.

    abstract::Acinetobacter pittii is an important pathogen causing nosocomial infection worldwide. In this study, a multidrug-resistant A. pittii ABC38 was used as host bacterium to isolate the lytic phage vB_ApiP_XC38. The biological characteristics of vB_ApiP_XC38 were studied and the genome was sequenced and analyzed. vB_ApiP_X...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Cheng M,Luo M,Xi H,Zhao Y,Le S,Chen LK,Tan D,Guan Y,Wang T,Han W,Wu N,Zhu T,Gu J

    更新日期:2020-08-01 00:00:00

  • First detection and complete genome sequence of Deformed wing virus in Chilean honeybees.

    abstract::Deformed wing virus (DWV) is one of the most common viruses affecting honey bee specimens. Although the presence of DWV has been reported in many countries, there is no data of the current situation in Chile. In this report, we detected the presence of DWV in apiaries from two different locations in central Chile. Fur...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Barriga GP,Cifuentes-Muñoz N,Rivera PA,Gutierrez M,Shmaryahu A,Valenzuela PD,Engel EA

    更新日期:2012-12-01 00:00:00

  • Evolution of viral DNA-dependent DNA polymerases.

    abstract::DNA viruses as their host cells require a DNA-dependent DNA polymerase (Pol) to faithfully replicate their genomic information. Large eukaryotic DNA viruses as well as bacterial viruses encode a specific Pol equipped with a proofreading 3'-5'-exonuclease, and other replication proteins. All known viral Pol belong to f...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Knopf CW

    更新日期:1998-01-01 00:00:00

  • Host-pathogen interactome analysis of Chikungunya virus envelope proteins E1 and E2.

    abstract::The envelope proteins of Chikungunya virus (CHIKV) are known to play crucial roles in viral infection and spread. Although the role of envelope proteins in viral infection has been studied, the cellular interactors of these proteins are still elusive. In the present study, the ectodomains of CHIKV envelope proteins (E...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Dudha N,Rana J,Rajasekharan S,Gabrani R,Gupta A,Chaudhary VK,Gupta S

    更新日期:2015-04-01 00:00:00

  • The genome of phiAsp2, an actinoplanes infecting phage.

    abstract::The first genome of a virus infecting a representative of the eubacterial genus Actinoplanes is presented. Phage phiAsp2 has a circularly permutated chromosome that consists of 58,638 bp; its G/C-bias of 70.39% resembles the hosts G + C-content (71-73% within the genus). A total of 76 open reading frames (orfs) were i...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Jarling M,Bartkowiak K,Pape H,Meinhardt F

    更新日期:2004-08-01 00:00:00

  • Sequence similarities and evolutionary relationships of influenza virus A hemagglutinins.

    abstract::This study brings the analysis of amino acid sequences of hemagglutinin (HA) from the influenza virus A that can infect a wide variety of birds and mammals. 191 sequences belonging to all known 15 HA subtypes were compared. The emphasis was given on functional sites (receptor-binding cavity with its right and left edg...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Kovácová A,Ruttkay-Nedecký G,Haverlík IK,Janecek S

    更新日期:2002-01-01 00:00:00

  • The use of bovine MHC class I allele-specific peptide motifs and proteolytic cleavage specificities for the prediction of potential cytotoxic T lymphocyte epitopes of bovine viral diarrhea virus.

    abstract::Cell mediated immunity (CMI) is crucial for the defense against viruses. Cytotoxic T lymphocytes (CTLs) play a major role in CMI. They recognize endogenous antigenic peptides presented by antigen presenting cells in association with the major histocompatibility complex (MHC) class I molecules. The elucidation of the s...

    journal_title:Virus genes

    pub_type: 杂志文章


    authors: Hegde NR,Srikumaran S

    更新日期:1997-01-01 00:00:00