A human vaccine strain of lamb rotavirus (Chinese) NSP4 gene: complete nucleotide sequence and phylogenetic analyses.

Abstract:

:A lamb strain of rotavirus has recently been licensed for use in China as a live vaccine to prevent rotavirus diarrhea in children. As rotavirus NSP4, especially the cytotoxic domain alone is considered to be associated with diarrhea, we sequenced gene segment 10, which encodes NSP4, of lamb rotavirus. Comparative analyses was performed to identify differences from human rotavirus strains, that might be associated with attenuation, and to ascertain whether the lamb rotavirus gene fits among the NSP4 of other sequenced rotavirus strains. Our comparative nucleotide sequence analysis suggests its close identity (91.17% homology) with that of group-A equine rotavirus (strain HI23). Multiple alignment of the deduced amino acid sequence of lamb NSP4 with that of other group A rotaviruses demonstrated homology ranging from 63.42% with that of porcine YM strain to 93.71% with equine HI23 strain of rotavirus. A group A-specific NSP4 monoclonal antibody recognized the glycosylated and unglycosylated forms of the protein from virus-infected lysates, suggesting a well-conserved group-specificity of the lamb NSP4. Phylogenetic analysis of the lamb rotavirus gene, with 60 other NSP4 gene sequences of human and animal rotavirus strains, demonstrated that the lamb rotavirus strain belongs to genotype A. Comparative analysis also revealed that although it is a vaccine strain, the NSP4 cytotoxic domain of lamb strain demonstrated an overall amino acid conservation similar to that of other strains, whose NSP4 alone causes diarrhea in animal models. These results taken together with our previous observations clearly reaffirm the idea that the attenuation phenotype of rotaviruses does not involve NSP4 cytotoxic domain, perhaps due to the suppression of NSP4 cytotoxic activity by other rotaviral proteins.

journal_name

Virus Genes

journal_title

Virus genes

authors

Mohan KV,Kulkarni S,Glass RI,Zhisheng B,Atreya CD

doi

10.1023/a:1023491514820

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

185-92

issue

2

eissn

0920-8569

issn

1572-994X

journal_volume

26

pub_type

杂志文章
  • Complete nucleotide sequences and construction of full-length infectious cDNA clones of cucumber green mottle mosaic virus (CGMMV) in a versatile newly developed binary vector including both 35S and T7 promoters.

    abstract::Seed-transmitted viruses have caused significant damage to watermelon crops in Korea in recent years, with cucumber green mottle mosaic virus (CGMMV) infection widespread as a result of infected seed lots. To determine the likely origin of CGMMV infection, we collected CGMMV isolates from watermelon and melon fields a...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-016-1415-x

    authors: Park CH,Ju HK,Han JY,Park JS,Kim IH,Seo EY,Kim JK,Hammond J,Lim HS

    更新日期:2017-04-01 00:00:00

  • Genetic analysis of the PB1-F2 gene of equine influenza virus.

    abstract::Amino acid and phylogenetic analysis showed that PB1-F2 of H7N7 and H3N8 equine influenza virus (EIV) evolved into two and nine clades, respectively. The PB1-F2 gene of H7N7 EIV strains isolated after 1973 was identified as being replaced by that of H3N8 EIV circulating simultaneously. All H7N7 EIV strains before 1973...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-0935-x

    authors: Lu G,Guo W,Qi T,Ma J,Zhao S,Tian Z,Pan J,Zhu C,Wang X,Xiang W

    更新日期:2013-10-01 00:00:00

  • Analysis of a chitinase from EpapGV, a fast killing betabaculovirus.

    abstract::The main function of baculoviral chitinase protein (V-CHIA) is to promote the final liquefaction of infected host larvae, facilitating the dispersion of occlusion bodies (OBs) in the environment. In this study, a v-chiA from Epinotia aporema Granulovirus (EpapGV) was identified and characterized. The 1,713 base pairs ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-1019-7

    authors: Salvador R,Ferrelli ML,Sciocco-Cap A,Romanowski V

    更新日期:2014-04-01 00:00:00

  • Expression of the X protein of hepatitis B virus in insect cells using recombinant baculoviruses.

    abstract::The baculovirus system was used to express the X protein of human hepatitis B virus (HBV). The X open reading frames (X ORFs) from cloned viral DNA of the HBV subtypes ayw and adr were introduced into the genome of Autographa californica nuclear polyhedrosis virus (AcNPV). The HBV-DNA of subtype adr derived from a hep...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF00571930

    authors: Klein R,Schröder CH,Zentgraf H

    更新日期:1991-04-01 00:00:00

  • Identification and characterization of the UL24 gene product of herpes simplex virus type 2.

    abstract::The UL24 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 281 amino acid protein with a molecular mass of 30.5 kDa. In this study, the HSV-2 UL24 gene product has been identified by using a rabbit polyclonal antiserum produced against a recombinant protein containing the full-length UL24 gene produ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1011118424474

    authors: Hong-Yan Z,Murata T,Goshima F,Takakuwa H,Koshizuka T,Yamauchi Y,Nishiyama Y

    更新日期:2001-06-01 00:00:00

  • Complete nucleotide sequence of a new strain of Tobacco necrosis virus A infecting soybean in China and infectivity of its full-length cDNA clone.

    abstract::The complete nucleotide sequence of a virus isolated from soybean (Glycine max (L.) Merr.) in China, previously identified as a new strain of Tobacco necrosis virus A (TNV-A) based on its biological, serological properties, and coat protein (CP) sequence and named as TNV-A C, was determined and compared with that of T...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-007-0185-x

    authors: Xi D,Li J,Han C,Li D,Yu J,Zhou X

    更新日期:2008-02-01 00:00:00

  • Genomic characterization of a slow/low maedi visna virus.

    abstract::The complete genomic sequence of a sheep lentivirus isolate that presents a slow/low phenotype in vitro has been determined. The virus, designated P1OLV, was isolated from lung cells of a naturally infected sheep in Portugal. Three overlapping DNA fragments amplified by PCR, and encompassing the entire viral genome we...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/B:VIRU.0000036380.01957.37

    authors: Barros SC,Ramos F,Duarte M,Fagulha T,Cruz B,Fevereiro M

    更新日期:2004-10-01 00:00:00

  • Polymerase chain-reaction-mediated cloning and expression of the coat protein gene of potato virus Y in Escherichia coli.

    abstract::Complementary DNAs (cDNAs) to the RNA genome of a necrotic strain of potato virus Y in Japan (Hokkaido Univ. isolate of PVY-T:PVY-TH) were synthesized and cloned into a plasmid pBR322. About 4.3 kbp of the cDNA sequence containing the 3'-poly(A) tract of PVY-TH was inserted into a recombinant plasmid pBRYT88. The coat...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF00570028

    authors: Hataya T,Sano T,Ohshima K,Shikata E

    更新日期:1990-12-01 00:00:00

  • Nucleotide sequence of the nucleoprotein gene of the RC.HL strain of rabies virus, a seed strain used for animal vaccine production in Japan.

    abstract::By using a phage vector (lambda ZAP II) and the mRNA extracted from IMR-32 cells infected with the RC.HL strain of rabies virus, we constructed a cDNA library from which four nucleoprotein (N)-specific cDNA clones were obtained by Southern blot hybridization. These clones contained a cDNA insert of about 1.4 kb, in wh...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF01703607

    authors: Goto H,Minamoto N,Ito H,Sugiyama M,Kinjo T,Mannen K,Mifune K,Kawai A

    更新日期:1994-03-01 00:00:00

  • Complete genome sequence of a novel Plum pox virus strain W isolate determined by 454 pyrosequencing.

    abstract::The near-complete (99.7 %) genome sequence of a novel Russian Plum pox virus (PPV) isolate Pk, belonging to the strain Winona (W), has been determined by 454 pyrosequencing with the exception of the thirty-one 5'-terminal nucleotides. This region was amplified using 5'RACE kit and sequenced by the Sanger method. Genom...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-0946-7

    authors: Sheveleva A,Kudryavtseva A,Speranskaya A,Belenikin M,Melnikova N,Chirkov S

    更新日期:2013-10-01 00:00:00

  • A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens.

    abstract::In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and TGEV. In a ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-016-1405-z

    authors: Zhu Y,Liang L,Luo Y,Wang G,Wang C,Cui Y,Ai X,Cui S

    更新日期:2017-02-01 00:00:00

  • Genomic analysis of a recombinant NADC30-like porcine reproductive and respiratory syndrome virus in china.

    abstract::Recently, NADC30-like porcine reproductive and respiratory syndrome viruses (PRRSVs), which are genetically similar to the NADC30 strain isolated in the United States of America in 2008, have become prevalent in China. Here, a novel variant PRRSV strain named HNhx was successfully isolated on porcine alveolar macropha...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-017-1516-1

    authors: Wang LJ,Wan B,Guo Z,Qiao S,Li R,Xie S,Chen XX,Zhang G

    更新日期:2018-02-01 00:00:00

  • The minimal sequence essential for replication and movement of Cotton leaf curl Multan betasatellite DNA by a helper virus in plant cells.

    abstract::Betasatellites are single-stranded circular DNAs associated with a number of monopartite begomoviruses. Betasatellites rely on the helper begomoviruses for replication and movement in plant tissues and plant-to-plant transmission by vectors. Their genomes are approximately half the size of the helper viruses and consi...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-016-1354-6

    authors: Eini O,Behjatnia SA

    更新日期:2016-10-01 00:00:00

  • The DI-DII linker of human parainfluenza virus type 3 fusion protein is critical for the virus.

    abstract::Human parainfluenza virus type 3 (HPIV3) causes the majority of childhood viral pneumonia around the world. Fusing the viral and target cell membranes is crucial for its entry into target cells, and the fusion process requires the concerted actions of two viral glycoproteins: hemagglutinin-neuraminidase (HN) and fusio...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-019-01713-8

    authors: Liu Y,Chi M,Wen H,Zhao L,Song Y,Liu N,Chi L,Wang Z

    更新日期:2020-02-01 00:00:00

  • The PDZ domain binding motif (PBM) of human T-cell leukemia virus type 1 Tax can be substituted by heterologous PBMs from viral oncoproteins during T-cell transformation.

    abstract::Several tumor viruses, such as human T-cell leukemia virus (HTLV), human papilloma virus (HPV), human adenovirus, have high-oncogenic and low-oncogenic subtypes, and such subtype-specific oncogenesis is associated with the PDZ-domain binding motif (PBM) in their transforming proteins. HTLV-1, the causative agent of ad...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-009-0447-x

    authors: Aoyagi T,Takahashi M,Higuchi M,Oie M,Tanaka Y,Kiyono T,Aoyagi Y,Fujii M

    更新日期:2010-04-01 00:00:00

  • Genetic analysis of H3 subtype influenza viruses isolated from domestic ducks in northern China during 2004-2005.

    abstract::The broad distribution and prevalence of H3 subtype influenza viruses in avian and mammalian hosts constitutes a global threat to both human and veterinary health. In this present study, six H3N8 influenza viruses isolated from domestic ducks during 2004-2005 in northern China were genetically and phylogenetically cha...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-008-0300-7

    authors: Pu J,Liu QF,Xia YJ,Fan YL,Brown EG,Tian FL,Liu JH

    更新日期:2009-02-01 00:00:00

  • Expression of VP2 gene protein of infectious bursal disease virus detected in Korea.

    abstract::The VP2 gene DNA (1.4 kb in approximate) of a very virulent infectious bursal disease virus (vvIBDV) Chinju strain detected in Chinju, Korea was cloned into the bacmid, a baculovirus shuttle vector, through transposition of the gene from initially cloned pFastBacHTa plasmid, a baculovirus expression vector, and was su...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1025128619976

    authors: Kim TK,Yeo SG

    更新日期:2003-08-01 00:00:00

  • Novel parvovirus sublineage in the family of Parvoviridae.

    abstract::Parvoviridae, which are classified into two subfamilies Parvovirinae and Densovirinae, can infect both vertebrate and insects and are related to a wide range of diseases in insects, animals, and humans. In this report, several new parvoviruses were identified in swine sera collected in southeastern China. The sequence...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-010-0506-3

    authors: Wang F,Wei Y,Zhu C,Huang X,Xu Y,Yu L,Yu X

    更新日期:2010-10-01 00:00:00

  • Characterization of H5N1 highly pathogenic avian influenza viruses isolated from poultry in Pakistan 2006-2008.

    abstract::Nine avian influenza viruses (AIV), H5N1 subtype, were isolated from dead poultry in the Karachi region of Pakistan from 2006 to 2008. The intravenous pathogenicity indices and HA protein cleavage sites of all nine viruses were consistent with highly pathogenic AIV. Based on phylogenetic analysis of the HA genes, thes...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-011-0689-2

    authors: Wasilenko JL,Pantin-Jackwood M,Khan TA,Ahmed A,Rehmani SF,Lone NA,Swayne DE,Spackman E

    更新日期:2012-04-01 00:00:00

  • European and American subgroup C isolates of avian metapneumovirus belong to different genetic lineages.

    abstract::The gene encoding the attachment glycoprotein (G) was sequenced in three French isolates of-subgroup C avian metapneumovirus (APV-C) from ducks. With 1771 nt, this gene proved as long as recently published for North-American APV-C isolates from turkeys. The nt sequences of the duck viruses shared 99% identity but prov...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-005-5850-3

    authors: Toquin D,Guionie O,Jestin V,Zwingelstein F,Allee C,Eterradossi N

    更新日期:2006-02-01 00:00:00

  • Base usage and dinucleotide frequency of infectious bursal disease virus.

    abstract::Base usage and dinucleotide frequency have been extensively studied in many eukaryotic organisms and bacteria, but not for viruses. In this paper, a comprehensive analysis of these aspects for infectious bursal disease virus (IBDV) was presented. The analysis of base usage indicated that all of the IBDV genes possess ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/B:VIRU.0000012262.89898.c7

    authors: Tan DY,Hair Bejo M,Aini I,Omar AR,Goh YM

    更新日期:2004-01-01 00:00:00

  • Genetic characterization of an isolate of canine distemper virus from a Tibetan Mastiff in China.

    abstract::Canine distemper (CD) is a highly contagious, often fatal, multisystemic, and incurable disease in dogs and other carnivores, which is caused by canine distemper virus (CDV). Although vaccines have been used as the principal means of controlling the disease, CD has been reported in vaccinated animals. The hemoagglutin...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-014-1062-z

    authors: Li W,Li T,Liu Y,Gao Y,Yang S,Feng N,Sun H,Wang S,Wang L,Bu Z,Xia X

    更新日期:2014-08-01 00:00:00

  • Trends of subtype variation of human immunodeficiency virus type 1 in Zhejiang Province, China.

    abstract::The epidemic characteristics of human immunodeficiency virus type 1 (HIV-1) in Zhejiang Province have not been systematically identified. This study presented a dynamic analysis of HIV-1 subtype variation in Zhejiang from 2004 to 2008, based on the surveillance of molecular epidemiology or drug resistance. CRF01_AE wa...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-014-1136-y

    authors: Yao Y,Xin R,Yang J,Xu Y,Zhang J,Xia Y,Pan X,Guo Z,Song Y

    更新日期:2015-02-01 00:00:00

  • A lipase-like gene from Heliothis virescens ascovirus (HvAV-3e) is essential for virus replication and cell cleavage.

    abstract::A unique feature of ascovirus infection is cleavage of host cells into virus containing vesicles. It has been suggested that the virus induces apoptosis, either by expression of a caspase or other means, which is then diverted toward vesicle formation. There is little known about the mechanism of vesicle formation. Re...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-009-0407-5

    authors: Smede M,Hussain M,Asgari S

    更新日期:2009-12-01 00:00:00

  • Transcriptional suppression of IE180 and TK promoters by the EP0 of pseudorabies virus strains Ea and Fa.

    abstract::In our transient expression assays, the IE180 and TK promoters were markedly suppressed by the EP0 of the pseudorabies virus strains Ea and Fa (EP0/Ea, EP0/Fa). This is in contrast with the transactivating activity of EP0 from strain YS-81 (EP0/YS-81) in previous studies. Amino acid sequence alignment revealed several...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-008-0320-3

    authors: Guo H,Zhou R,Xi Y,Xiao S,Chen H

    更新日期:2009-04-01 00:00:00

  • Macropodid herpesvirus 1 encodes genes for both thymidylate synthase and ICP34.5.

    abstract::Macropodid herpesvirus 1 (MaHV-1) is an unclassified alphaherpesvirus linked with the fatal infections of kangaroos and other marsupials. During the characterisation of the internal repeat region of MaHV-1, an open reading frame (ORF) encoding for thymidylate synthase (TS) gene was identified and completely sequenced....

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1015396430131

    authors: Guliani S,Polkinghorne I,Smith GA,Young P,Mattick JS,Mahony TJ

    更新日期:2002-06-01 00:00:00

  • Genomic characterization of a novel partitivirus infecting. Aspergillus ochraceus.

    abstract::Three double-stranded RNA (dsRNA) segments from Aspergillus ochraceus isolate FA 0611, designated as AoR1, AoR2, and AoR3, were cloned and sequenced. AoR1 was identical with AoV dsRNA 1 previously reported from A. ochraceus ATCC 28706, which putatively encoded an RNA-dependent RNA polymerase of an unidentified mycovir...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-008-0265-6

    authors: Liu W,Duns G,Chen J

    更新日期:2008-12-01 00:00:00

  • First detection and complete genome sequence of Deformed wing virus in Chilean honeybees.

    abstract::Deformed wing virus (DWV) is one of the most common viruses affecting honey bee specimens. Although the presence of DWV has been reported in many countries, there is no data of the current situation in Chile. In this report, we detected the presence of DWV in apiaries from two different locations in central Chile. Fur...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-012-0791-0

    authors: Barriga GP,Cifuentes-Muñoz N,Rivera PA,Gutierrez M,Shmaryahu A,Valenzuela PD,Engel EA

    更新日期:2012-12-01 00:00:00

  • Assessing circovirus gene flow in multiple spill-over events.

    abstract::The establishment of viral pathogens in new host environments following spillover events probably requires adaptive changes within both the new host and pathogen. After many generations, signals for ancient cross-species transmission may become lost and a strictly host-adapted phylogeny may mimic true co-divergence wh...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-019-01702-x

    authors: Das S,Smith K,Sarker S,Peters A,Adriaanse K,Eden P,Ghorashi SA,Forwood JK,Raidal SR

    更新日期:2019-12-01 00:00:00

  • Establishment of mouse leukemia cell lines expressing human CD4/CCR5 using lentiviral vectors.

    abstract::A low-cost rodent model of HIV infection and which presents high application value is an effective tool to investigate HIV infection and pathogenesis. However, development of such a small animal model has been hampered by the unsuitability of rodent cells for HIV-1 replication given that the retrovirus HIV-1 has high ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-016-1423-x

    authors: Li YJ,ZhuGe FY,Zeng CC,He JY,Tan N,Liang J

    更新日期:2017-04-01 00:00:00