Abstract:
:The incidence of watermelon chlorotic stunt disease and the molecular characterization of the Jordanian isolate of Watermelon chlorotic stunt virus (WmCSV-[JO]) are described in this study. Symptomatic leaf samples obtained from watermelon (Citrullus lanatus Thunb.), melon (Cucumis melo L.), squash (Cucurbita pepo), cucumber (Cucumis sativus L.), and bottle gourd (Lagenaria siceraria) plants were tested for WmCSV-[JO] infection by PCR. The virus could be detected in 8 melon and 87 watermelon samples obtained from Ghor Assafi (southern part of Jordan Valley). Three samples collected from Mafraq (eastern part of Jordan) were found mixed infected with WmCSV-[JO] and Squash leaf curl virus. The full-length DNA-A and DNA-B genomes of WmCSV-[JO] were amplified, and sequences were deposited in the GenBank under accession numbers EU561237 and EU561236, respectively. Sequence analysis reveals that WmCSV-[JO] is closely related to other virus isolates from Israel (WmCSV-[IL]), Yemen (WmCSV-[YE]), Iran (WmCSV-[IR]), Lebanon (WmCSV-[LB]), and Sudan (WmCSV-[SD]). DNA-A of WmCSV-[JO] showed highest nucleotide identity (99.42%) with WmCSV-[IL], while DNA-B had highest nucleotide identity (95.52%) with WmCSV-[YE]. Data of this study demonstrate that digestion of DNA-B genome of WmCSV isolates with ApaI enzyme can discriminate between these isolates at the molecular level. Infectious clones of WmCSV-[JO] were constructed and agroinoculated to Nicotiana benthamiana plants. Inoculated plants developed mild disease symptoms 4 weeks post inoculation, while watermelon plants biolistically inoculated with WmCSV-[JO] developed characteristic mottling, yellowing and severe leaf curling symptoms 3 weeks post inoculation.
journal_name
Virus Genesjournal_title
Virus genesauthors
Al-Musa A,Anfoka G,Al-Abdulat A,Misbeh S,Haj Ahmed F,Otri Idoi
10.1007/s11262-011-0594-8subject
Has Abstractpub_date
2011-08-01 00:00:00pages
79-89issue
1eissn
0920-8569issn
1572-994Xjournal_volume
43pub_type
杂志文章相关文献
VIRUS GENES文献大全abstract::Recently, NADC30-like porcine reproductive and respiratory syndrome viruses (PRRSVs), which are genetically similar to the NADC30 strain isolated in the United States of America in 2008, have become prevalent in China. Here, a novel variant PRRSV strain named HNhx was successfully isolated on porcine alveolar macropha...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-017-1516-1
更新日期:2018-02-01 00:00:00
abstract::Pneumonia virus of mice (PVM) is an enveloped RNA-containing virus of Family Paramyxoviridae. Sequences had been determined previously for a number of PVM genes, although these represented cloned cDNAs rather than consensus sequences. Sequences were not available for the 3' -leader and 5' -trailer regions that constit...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-004-5631-4
更新日期:2005-03-01 00:00:00
abstract::A unique feature of ascovirus infection is cleavage of host cells into virus containing vesicles. It has been suggested that the virus induces apoptosis, either by expression of a caspase or other means, which is then diverted toward vesicle formation. There is little known about the mechanism of vesicle formation. Re...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0407-5
更新日期:2009-12-01 00:00:00
abstract::A new, widespread disease was recently observed in soybean in the United States. The disease, named Soybean vein necrosis, is manifested by intraveinal chlorosis and necrosis, and has been found in almost all of the 50 fields visited over a period of 3 years in the midwest and midsouth part of the United States. A vir...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-011-0621-9
更新日期:2011-10-01 00:00:00
abstract::A lamb strain of rotavirus has recently been licensed for use in China as a live vaccine to prevent rotavirus diarrhea in children. As rotavirus NSP4, especially the cytotoxic domain alone is considered to be associated with diarrhea, we sequenced gene segment 10, which encodes NSP4, of lamb rotavirus. Comparative ana...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1023491514820
更新日期:2003-01-01 00:00:00
abstract::White spot syndrome virus (WSSV) is one of the major pathogens of cultured shrimp. Identification of envelope protein interactions has become a central issue for the understanding of WSSV assembly. In this paper, WSSV envelope protein VP52B was fused with GST-tag and expressed in Escherichia coli BL-21(DE3). Immunogol...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-014-1126-0
更新日期:2015-02-01 00:00:00
abstract::The sequence of rice hoja blanca tenuivirus RNA-2 is analysed and compared to its counter-part in rice stripe tenuivirus. The RNA encodes two proteins, in an ambisense arrangement. The 94 kD pc2, located in the complementary sense RNA, has several features typical of viral membrane (glyco)proteins, and also has region...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00284643
更新日期:1996-01-01 00:00:00
abstract::A bovine viral diarrhea disease virus (BVDV) GS-4 was isolated in Western China form dairy cattle with respiratory disease. Genomic comparison analysis with the 5' half genome sequence encompassing the coding region of N(pro), capsid, and envelope glycoproteins showed that the GS-4 should be classified into BVDV-1b1, ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-0917-z
更新日期:2013-08-01 00:00:00
abstract::Cap-independent translations of viral RNAs of enteroviruses and rhinoviruses, cardioviruses and aphthoviruses, hepatitis A and C viruses (HAV and HCV), and pestivirus are initiated by the direct binding of 40S ribosomal subunits to a cis-acting genetic element termed the internal ribosome entry site (IRES) or ribosome...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00572952
更新日期:1996-01-01 00:00:00
abstract::Bovine viral diarrhea virus (BVDV) belongs to the Pestivirus genus of the Flaviviridae family and has worldwide distribution, being one of the main causes of economic losses in cattle raising. The genome of pestiviruses is a single strand of positive-sense RNA with a length of 12.3 kb, which encodes one open reading f...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-020-01756-2
更新日期:2020-08-01 00:00:00
abstract::The main function of baculoviral chitinase protein (V-CHIA) is to promote the final liquefaction of infected host larvae, facilitating the dispersion of occlusion bodies (OBs) in the environment. In this study, a v-chiA from Epinotia aporema Granulovirus (EpapGV) was identified and characterized. The 1,713 base pairs ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-1019-7
更新日期:2014-04-01 00:00:00
abstract::The simian virus 40 (SV40) large T antigen is a 708 amino-acid protein possessing multiple biochemical activities that play distinct roles in productive infection or virus-induced cell transformation. The carboxy-terminal portion of T antigen includes a domain that carries the nucleotide binding and ATPase activities ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1007941622680
更新日期:1998-01-01 00:00:00
abstract::The varicella zoster virus (VZV) ORF4 protein, one of immediate-early genes protein, is associated with the tegument in purified virions. ORF4 protein functions at both transcriptional and post-transcriptional levels, present during different phase of whole VZV life cycle. ORF4 protein acts as a nucleocytoplasm shuttl...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-1006-z
更新日期:2014-04-01 00:00:00
abstract::African monkeys are resistant to HIV-1 infection due to intrinsic restriction mechanisms found in their cells. However, although they can be infected by monkey-adapted modified HIV-1 particles that are designed to overcome known restriction factors, virus numbers drop to undetectable levels in immunocompetent animals....
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-020-01731-x
更新日期:2020-04-01 00:00:00
abstract::Coxsackieviruses are important human pathogens that induce myocarditis and pancreatitis. However, there are no vaccines or therapeutic reagents for their clinical treatment. Although RNA interference (RNAi)-based approaches to the prevention of viral production have been developed recently, limitations to the in vivo ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-007-0192-y
更新日期:2008-02-01 00:00:00
abstract::The sequence is presented of RNA-4 of Echinochloa hoja blanca tenuivirus (EHBV), one of two tenuiviruses associated with rice cultivation in Latin America (together with rice hoja blanca virus; RHBV). Analysis of the sequence shows that the coding regions of EHBV RNA-4 are closely related to those of RHBV RNA-4. Howev...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00576979
更新日期:1996-01-01 00:00:00
abstract::The broad distribution and prevalence of H3 subtype influenza viruses in avian and mammalian hosts constitutes a global threat to both human and veterinary health. In this present study, six H3N8 influenza viruses isolated from domestic ducks during 2004-2005 in northern China were genetically and phylogenetically cha...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-008-0300-7
更新日期:2009-02-01 00:00:00
abstract::Comparison of the genomic organization of variola and vaccinia viruses has been carried out. Molecular factors of virulence of these viruses is the focus of this review. Possible roles of the genes of soluble cytokine receptors, complement control proteins, factors of virus replication, and dissemination in vivo for v...
journal_title:Virus genes
pub_type: 杂志文章,评审
doi:10.1007/BF01724297
更新日期:1995-01-01 00:00:00
abstract::Complementary DNAs (cDNAs) to the RNA genome of a necrotic strain of potato virus Y in Japan (Hokkaido Univ. isolate of PVY-T:PVY-TH) were synthesized and cloned into a plasmid pBR322. About 4.3 kbp of the cDNA sequence containing the 3'-poly(A) tract of PVY-TH was inserted into a recombinant plasmid pBRYT88. The coat...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00570028
更新日期:1990-12-01 00:00:00
abstract::The Agrotis ipsilon multiple nucleopolyhedrovirus (AgipMNPV) is a group II nucleopolyhedrovirus (NPV) from the black cutworm, A. ipsilon, with potential as a biopesticide to control infestations of cutworm larvae. The genome of the Illinois strain of AgipMNPV was completely sequenced. The AgipMNPV genome was 155,122 n...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-008-0297-y
更新日期:2009-02-01 00:00:00
abstract::One of the consequences of equine herpesvirus 1 (EHV-1) infection in the natural host is a neurological disease that can lead to paralysis. The pathology associated with EHV-1-induced neurological disease includes vasculitis of the small blood vessels within the central nervous system and subsequent damage to the surr...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/B:VIRU.0000032785.19420.14
更新日期:2004-08-01 00:00:00
abstract::Viroids, the smallest and simplest agents of infectious disease, cause a number of economically important diseases of crop plants. Present evidence indicates that most of these diseases originated recently (in the 20th century) by chance transfer of viroids from endemically infected wild plants or by use of viroid-inf...
journal_title:Virus genes
pub_type: 杂志文章,评审
doi:10.1007/BF01728653
更新日期:1995-01-01 00:00:00
abstract::Chinese equine infectious anemia virus (EIAV) attenuated vaccine is the first lentiviral vaccine with a successful application. In order to understand the correlation of viral genomic mutations with viral attenuation and with induced immunoprotective properties, we analyzed the proviral genome sequences of the EIAV-at...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-010-0491-6
更新日期:2010-08-01 00:00:00
abstract::We have examined sequence variability among nine isolates of hop stunt viroid (HSVd) collected from hop gardens in Tohoku district in Japan, the only area in the world where hop stunt disease is endemic. Six different consensus and one-consensus sequences as well as 12 sequence variants were detected in the nine HSVd-...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008182302704
更新日期:2001-01-01 00:00:00
abstract::The thymidylate synthase (TS, EC 2.1.1.45) is essential for the de novo synthesis of dTMP in pro- and eucaryotic organisms. Consequently it plays a major role in the replication of the DNA genome of a cell or a DNA virus. The gene encoding the TS of Chilo iridescent virus (CIV) was identified by nucleotide sequence an...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008017820941
更新日期:1998-01-01 00:00:00
abstract::The infectious bronchitis virus (IBV) spike glycoprotein S1 subunit is required to initiate infection and contains virus-neutralizing and serotype-specific epitope(s). Reported are the S1 gene nucleotide and predicted amino acid sequences for the Florida 18288 strain and isolates GA-92, CV-56b, CV-9437, CV-1686, and 1...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008057118625
更新日期:1998-01-01 00:00:00
abstract::The establishment of viral pathogens in new host environments following spillover events probably requires adaptive changes within both the new host and pathogen. After many generations, signals for ancient cross-species transmission may become lost and a strictly host-adapted phylogeny may mimic true co-divergence wh...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-019-01702-x
更新日期:2019-12-01 00:00:00
abstract::Schmallenberg virus (SBV) is the member of Peribunyaviridae family, which comprises pathogens of importance for human and veterinary medicine. The virus is transmitted only between animals and mainly by biting midges of the genus Culicoides. This study was performed in order to determine SBV genetic diversity and eluc...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-018-1607-7
更新日期:2018-12-01 00:00:00
abstract::Canine distemper virus (CDV) causes a highly contagious disease in a wide range of carnivores. The hemagglutinin (H) protein of viruses shows the highest variability and plays an important role in modulation of viral antigenicity, virulence, and receptor recognition. Since 2012, canine distemper (CD) outbreaks in fur-...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-019-01720-9
更新日期:2020-02-01 00:00:00
abstract::Novel duck reovirus (NDRV), the prototype strain of the species Avian orthoreovirus (ARV), is associated with high mortality in Pekin ducklings. σC is an outer capsid protein encoded by the S1 genome segment of NDRV which mediates attachment to host cells. Our previous studies using immunoprecipitation and mass spectr...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-020-01750-8
更新日期:2020-06-01 00:00:00