Abstract：
:White spot syndrome virus (WSSV) is one of the major pathogens of cultured shrimp. Identification of envelope protein interactions has become a central issue for the understanding of WSSV assembly. In this paper, WSSV envelope protein VP52B was fused with GST-tag and expressed in Escherichia coli BL-21(DE3). Immunogold-electron microscopy revealed that VP52B was located on the outside surface of WSSV virions. Far-Western blotting analysis suggested that VP52B might directly interact with a major viral envelope protein VP26, and their interaction was confirmed by GST pull-down assay. Further investigation showed that the VP52B binding domain was located between residues 135-170 of VP26. These findings will enhance our understanding of the molecular mechanisms of WSSV morphogenesis.
journal_name
Virus Genesjournal_title
Virus genesauthors
Lin F,Jie Z,Hou L,Li F,Yang Fdoi
10.1007/s11262-014-1126-0subject
Has Abstractpub_date
2015-02-01 00:00:00pages
46-51issue
1eissn
0920-8569issn
1572-994Xjournal_volume
50pub_type
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