Abstract:
:White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. The viral early genes play a key role in DNA replication and virus proliferation. In this study, a WSSV gene (wsv477) encoding 208 amino acid peptides was characterized as an early gene. The temporal analysis showed that the wsv477 gene was first transcribed at 4 h post-infection, suggesting that it was an early gene. The wsv477 gene was expressed in Escherichia coli and purified. Subsequently the specific antibody was raised using the purified fusion protein (GST-WSV477). Western blot revealed that the wsv477 gene was expressed at 6 h post-infection in vivo. As indicated by GTP-binding assay, the WSV477 protein had GTP-binding activity.
journal_name
Virus Genesjournal_title
Virus genesauthors
Han F,Xu J,Zhang Xdoi
10.1007/s11262-006-0053-0subject
Has Abstractpub_date
2007-04-01 00:00:00pages
193-8issue
2eissn
0920-8569issn
1572-994Xjournal_volume
34pub_type
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