Characterization of an early gene (wsv477) from shrimp white spot syndrome virus (WSSV).

Abstract:

:White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. The viral early genes play a key role in DNA replication and virus proliferation. In this study, a WSSV gene (wsv477) encoding 208 amino acid peptides was characterized as an early gene. The temporal analysis showed that the wsv477 gene was first transcribed at 4 h post-infection, suggesting that it was an early gene. The wsv477 gene was expressed in Escherichia coli and purified. Subsequently the specific antibody was raised using the purified fusion protein (GST-WSV477). Western blot revealed that the wsv477 gene was expressed at 6 h post-infection in vivo. As indicated by GTP-binding assay, the WSV477 protein had GTP-binding activity.

journal_name

Virus Genes

journal_title

Virus genes

authors

Han F,Xu J,Zhang X

doi

10.1007/s11262-006-0053-0

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

193-8

issue

2

eissn

0920-8569

issn

1572-994X

journal_volume

34

pub_type

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