Emergence of rabbit haemorrhagic disease virus 2 in the archipelago of Madeira, Portugal (2016-2017).

Abstract:

:We report the detection of rabbit haemorrhagic disease virus 2 (RHDV2) in the Madeira archipelago, Portugal. Viral circulation was confirmed by RT-qPCR and vp60 sequencing. Epidemiological data revealed the outbreak initiated in October 2016 in Porto Santo affecting wild and domestic rabbits. It was then detected three months later on the island of Madeira. Five haplotypes were identified and a genetic overall similarity of 99.54 to 99.89% was observed between the two viral populations. Unique single nucleotide polymorphisms were recognised in the Madeira archipelago strains, two of which resulting in amino acid substitutions at positions 480 and 570 in the VP60 protein. Phylogenetic investigation by Maximum Likelihood showed all the vp60 sequences from the Madeira archipelago group together with high bootstraps. The analysis also showed that the Madeira archipelago strains are closely related to the strains detected in the south of mainland Portugal in 2016, suggesting a possible introduction from the mainland. The epidemiological data and high genetic similarity indicate a common source for the Porto Santo and Madeira RHDV2 outbreaks. Human activity related to hunting was most probably at the origin of the Madeira outbreak.

journal_name

Virus Genes

journal_title

Virus genes

authors

Carvalho CL,Silva S,Gouveia P,Costa M,Duarte EL,Henriques AM,Barros SS,Luís T,Ramos F,Fagulha T,Fevereiro M,Duarte MD

doi

10.1007/s11262-017-1483-6

subject

Has Abstract

pub_date

2017-12-01 00:00:00

pages

922-926

issue

6

eissn

0920-8569

issn

1572-994X

pii

10.1007/s11262-017-1483-6

journal_volume

53

pub_type

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