Complete genomic sequence and phylogenetic relatedness of hepatitis B virus isolates in Cambodia.

Abstract:

:Although it is known that Cambodia is one of the high endemic area of hepatitis B virus (HBV) infection, molecular characterization of HBV circulating in this country has not been reported. In this study, pre-S gene of HBV from 12 Cambodian patients was sequenced. Phylogenetic analysis based on the pre-S gene sequence revealed that 8 out of 12 isolates (66.7%) belonged to HBV/C1 and remaining four (33.3%) were HBV/B4. Furthermore, complete genomic sequences were also determined for three Cambodian HBV isolates. They all comprised of 3,215 bp long and two of them belonged to subgenotype B4, which had recombination event with genotype C in the precore/core gene confirmed by SimPlot and BootScanning analyses. Our results showed that both HBV strains belonged to subgenotypes B4 and C1, which are circulating in this country. This is the first report on molecular characterization of the HBV prevalent in Cambodia.

journal_name

Virus Genes

journal_title

Virus genes

authors

Huy TT,Sall AA,Reynes JM,Abe K

doi

10.1007/s11262-008-0205-5

subject

Has Abstract

pub_date

2008-04-01 00:00:00

pages

299-305

issue

2

eissn

0920-8569

issn

1572-994X

journal_volume

36

pub_type

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