Identification and characterization of the UL24 gene product of herpes simplex virus type 2.

Abstract:

:The UL24 gene of herpes simplex virus type 2 (HSV-2) is predicted to encode a 281 amino acid protein with a molecular mass of 30.5 kDa. In this study, the HSV-2 UL24 gene product has been identified by using a rabbit polyclonal antiserum produced against a recombinant protein containing the full-length UL24 gene product of HSV-2 fused to glutathione-S-transferase. The antiserum reacted specifically with a 32 kDa protein in HSV-2 186-infected Vero cells and with 31 and 32 kDa proteins in UL24-expressing Cos-7 cells. Accumulation of UL24 protein to detectable levels required viral DNA synthesis, indicating that the protein was regulated as a late gene. UL24 protein was found to be associated with purified HSV-2 virions and C capsids. Indirect immunofluorescence analysis demonstrated that the UL24-specific fluorescence was detected in perinuclear regions of the cytoplasm and/or in the nucleus as small discrete granules from 9h post infection (hpi). Furthermore, the UL24 protein expressed singly was detected predominantly in the nucleus and slightly in the cytoplasm at 24 h after transfection, with branch-like cytoplasmic protruding structures. Strong nucleolus staining was visible in partial cells.

journal_name

Virus Genes

journal_title

Virus genes

authors

Hong-Yan Z,Murata T,Goshima F,Takakuwa H,Koshizuka T,Yamauchi Y,Nishiyama Y

doi

10.1023/a:1011118424474

subject

Has Abstract

pub_date

2001-06-01 00:00:00

pages

321-7

issue

3

eissn

0920-8569

issn

1572-994X

journal_volume

22

pub_type

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