Abstract:
:Duck hepatitis A virus type 1, (DHAV-1) 2A2pro, is one of the most highly conserved viral proteins within the DHAV serotypes. However, its effect on host cells is unclear. We predicted that DHAV-1 2A2pro was a GTPase-like protein based on the results of multiple sequence alignment and homologous modeling analysis. Upon transfection of a recombinant plasmid expressing DHAV-1 2A2, cells displayed fragmented nuclei, chromatin condensation, oligonucleosome-sized DNA ladder, and positive terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling staining; hence, cell death has the characteristics of apoptosis. By staining cells with fluorescein Annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze nonapoptotic cells, early apoptotic cells, late apoptotic/necrotic cells, and dead cells through flow cytometry and fluorescence microscopy. The percentage of apoptotic cells gradually increased and reached a maximum after 48 h of transfection. In conclusion, apoptosis induced by this GTPase-like protein may contribute to DHAV-1 pathogenesis.
journal_name
Virus Genesjournal_title
Virus genesauthors
Cao J,Ou X,Zhu D,Ma G,Cheng A,Wang M,Chen S,Jia R,Liu M,Sun K,Yang Q,Wu Y,Chen Xdoi
10.1007/s11262-016-1364-4subject
Has Abstractpub_date
2016-12-01 00:00:00pages
780-788issue
6eissn
0920-8569issn
1572-994Xpii
10.1007/s11262-016-1364-4journal_volume
52pub_type
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