The 2A2 protein of Duck hepatitis A virus type 1 induces apoptosis in primary cell culture.

Abstract:

:Duck hepatitis A virus type 1, (DHAV-1) 2A2pro, is one of the most highly conserved viral proteins within the DHAV serotypes. However, its effect on host cells is unclear. We predicted that DHAV-1 2A2pro was a GTPase-like protein based on the results of multiple sequence alignment and homologous modeling analysis. Upon transfection of a recombinant plasmid expressing DHAV-1 2A2, cells displayed fragmented nuclei, chromatin condensation, oligonucleosome-sized DNA ladder, and positive terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling staining; hence, cell death has the characteristics of apoptosis. By staining cells with fluorescein Annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze nonapoptotic cells, early apoptotic cells, late apoptotic/necrotic cells, and dead cells through flow cytometry and fluorescence microscopy. The percentage of apoptotic cells gradually increased and reached a maximum after 48 h of transfection. In conclusion, apoptosis induced by this GTPase-like protein may contribute to DHAV-1 pathogenesis.

journal_name

Virus Genes

journal_title

Virus genes

authors

Cao J,Ou X,Zhu D,Ma G,Cheng A,Wang M,Chen S,Jia R,Liu M,Sun K,Yang Q,Wu Y,Chen X

doi

10.1007/s11262-016-1364-4

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

780-788

issue

6

eissn

0920-8569

issn

1572-994X

pii

10.1007/s11262-016-1364-4

journal_volume

52

pub_type

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