The characteristics and genome analysis of vB_ApiP_XC38, a novel phage infecting Acinetobacter pittii.

Abstract:

:Acinetobacter pittii is an important pathogen causing nosocomial infection worldwide. In this study, a multidrug-resistant A. pittii ABC38 was used as host bacterium to isolate the lytic phage vB_ApiP_XC38. The biological characteristics of vB_ApiP_XC38 were studied and the genome was sequenced and analyzed. vB_ApiP_XC38 belonged to Podoviridae family. The phage had double-stranded genome, which comprised 79,328 bp with 39.58% G+C content displaying very low similarity (< 1% identity) with published genomes of other phages and bacteria. A total of 97 open reading frames (ORFs) were predicted and contained nucleotide metabolism and replication module, structural components module, and lysis module. The ANI, AAI, and phylogenetic analysis indicated that all phages were found distant from vB_ApiP_XC38. Altogether, morphological, genomics, and phylogenetic analysis suggest that vB_ApiP_XC38 is more likely a novel phage of A. pittii.

journal_name

Virus Genes

journal_title

Virus genes

authors

Cheng M,Luo M,Xi H,Zhao Y,Le S,Chen LK,Tan D,Guan Y,Wang T,Han W,Wu N,Zhu T,Gu J

doi

10.1007/s11262-020-01766-0

subject

Has Abstract

pub_date

2020-08-01 00:00:00

pages

498-507

issue

4

eissn

0920-8569

issn

1572-994X

pii

10.1007/s11262-020-01766-0

journal_volume

56

pub_type

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