Abstract:
:The complete nucleotide sequence of a virus isolated from soybean (Glycine max (L.) Merr.) in China, previously identified as a new strain of Tobacco necrosis virus A (TNV-A) based on its biological, serological properties, and coat protein (CP) sequence and named as TNV-A C, was determined and compared with that of TNV-A and other closely related Necroviruses and Carmoviruses. The viral RNA genome consists of 3,682 nucleotides and contains five open reading frames (ORFs). TNV-A C showed 86.4% overall nucleotide sequence identity to TNV-A. The CP and putative RNA-dependent RNA polymerase (RdRp) showed 88.8 and 95.9% amino acid identity, respectively, to that of TNV-A. The greatest difference between TNV-A C and TNV-A was in the 3' terminal region: the p7K ORF region present in TNV-A was absent in TNV-A C. Phylogenetic analysis of RdRp, CP, and small ORF regions of Necroviruses confirmed TNV-A C as a new strain of TNV-A. A full-length cDNA clone of TNV-A C was constructed and used as template for run-off transcription based on the obtained sequence. The results indicate that the in vitro-synthesized viral RNA faithfully represented the biological activity of wild-type TNV-A C.
journal_name
Virus Genesjournal_title
Virus genesauthors
Xi D,Li J,Han C,Li D,Yu J,Zhou Xdoi
10.1007/s11262-007-0185-xsubject
Has Abstractpub_date
2008-02-01 00:00:00pages
259-66issue
1eissn
0920-8569issn
1572-994Xjournal_volume
36pub_type
杂志文章相关文献
VIRUS GENES文献大全abstract::Two cucumber mosaic virus (CMV) isolates XJ1 and XJ2 were obtained from sugar beet showing yellow mosaic symptom in Shihezi, Xinjiang Uigur municipality of China. The coat protein gene of the two CMV isolates and their associated satellite RNAs were amplified by reverse transcriptase polymerase chain reaction (RT-PCR)...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-006-0068-6
更新日期:2006-12-01 00:00:00
abstract::Twenty-three CSFV isolates recovered from field outbreaks in various parts of India during 2006-2009 were used for genetic analysis in the NS5B region (409 nts). Seventeen of these were studied earlier [16] in the 5'UTR region. Phylogenetic analysis indicated the continued dominance of subgroup 1.1 strains in the coun...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-011-0572-1
更新日期:2012-02-01 00:00:00
abstract::Porcine astrovirus (PAstV) belongs to genetically divergent lineages within the genus Mamastrovirus. In this study, 25/129 (19.4 %) domestic pig and 1/146 (0.7 %) wild boar fecal samples tested in South Korea were positive for PAstV. Positive samples were mainly from pigs under 6 weeks old. Bayesian inference (BI) tre...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-012-0816-8
更新日期:2013-02-01 00:00:00
abstract::Bovine viral diarrhea virus (BVDV) belongs to the Pestivirus genus of the Flaviviridae family and has worldwide distribution, being one of the main causes of economic losses in cattle raising. The genome of pestiviruses is a single strand of positive-sense RNA with a length of 12.3 kb, which encodes one open reading f...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-020-01756-2
更新日期:2020-08-01 00:00:00
abstract::Based on the genetic analysis, novel, naturally occurring recombinant norovirus strains were identified in Japan. Breakpoint analysis of recombinant norovirus showed that the recombination site was at the open reading frame ORF1/ORF2 overlap. ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-010-0459-6
更新日期:2010-06-01 00:00:00
abstract::A 13 amino acid residue insertion was found in the N-terminal region of the coat protein of several Maize dwarf mosaic virus isolates (MDMV). These insertions seem to be the result of a direct duplication event, but differ in some positions. In order to evaluate the influence of the insertion on the RNA secondary stru...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0425-3
更新日期:2010-02-01 00:00:00
abstract::The major immunogenic protein VP2 from a pathogenic field isolate (variant A virus) of infectious bursal disease virus (IBDV) was cloned and sequenced to examine antigenic variations. The VP2 open reading frame consists of 1509 nucleotides and codes for a 503 amino acid protein. Overall, the VP2 amino acid sequence of...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF01702563
更新日期:1992-08-01 00:00:00
abstract::The Chinese equine infectious anemia virus (EIAV) virulent strain EIAV(LN40) is derived from a naturally occurring virus by continuously passing in horses for 16 generations. Its genome sequence is 23% different from that of the American strains or the Japanese strains, and the variation of envelope gp90 surface unit ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-010-0563-7
更新日期:2011-04-01 00:00:00
abstract::Fowl adenovirus serotype 4 (FAdV-4) is the causative agent of hydropericardium syndrome (HPS), a highly pathogenic disease in poultry. In the present study, hexon, penton base, and fiber-2 genes encoding major capsid proteins were analyzed in four FAdV-4 isolates from HPS-affected chickens in Korea. Nucleotide sequenc...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1393-z
更新日期:2017-02-01 00:00:00
abstract::The envelope proteins of Chikungunya virus (CHIKV) are known to play crucial roles in viral infection and spread. Although the role of envelope proteins in viral infection has been studied, the cellular interactors of these proteins are still elusive. In the present study, the ectodomains of CHIKV envelope proteins (E...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-014-1161-x
更新日期:2015-04-01 00:00:00
abstract::The 5(-terminal leader sequence of the equine arteritis virus (EAV) genome contains an open reading frame (ORF) with an AUG codon in a suboptimal context for initiation of protein synthesis. To investigate the significance of this intraleader ORF (ILO), an expression plasmid was generated carrying a DNA copy of the su...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-005-0030-z
更新日期:2006-08-01 00:00:00
abstract::In 2009, a bovine parainfluenza virus (BPIV3), named as NM09, was isolated using MDBK cell culture from the nasal swabs of normal cattle in China. The NM09 isolate was characterized by RT-PCR and nucleotide sequence analysis. Its complete genome was 15,456 nucleotides in length. Similar to other sequenced PIV strains,...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-012-0810-1
更新日期:2012-12-01 00:00:00
abstract::Endogenous retrovirus-like sequences that are homologous to the multigenic murine and Syrian hamster intracisternal type A particle (IAP) genes can be detected in very few copies in the Chinese hamster (CH) genome. They were cloned from a CHO gene library and two recombinants, harboring the major IAP-like DNA genes ch...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00570029
更新日期:1990-12-01 00:00:00
abstract::Gallid herpesvirus-1 (GaHV-1), commonly named infectious laryngotracheitis (ILT) virus, causes the respiratory disease in chickens known as ILT. The molecular determinants associated with differences in pathogenicity of GaHV-1 strains are not completely understood, and a comparison of genomic sequences of isolates tha...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-011-0696-3
更新日期:2012-04-01 00:00:00
abstract::Cotton leaf curl disease (CLCuD) has been a major constraint to cotton production across Pakistan and northwestern India since the early 1990s. The disease is caused by a number of begomoviruses, including Cotton leaf curl Multan virus (CLCuMuV), which associate with a specific host range and symptom determining betas...
journal_title:Virus genes
pub_type: 信件
doi:10.1007/s11262-018-1605-9
更新日期:2018-12-01 00:00:00
abstract::The nucleotide sequence of the 3' terminal region of potato virus Y (PVY) was determined. Starting with a poly(A) tail of 18 residues a non-coding region of 335 nucleotides precedes the region encoding for the virus coat protein (cp) 801 nucleotides long ending with a TGA. This region was located by comparing the pred...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00572704
更新日期:1988-06-01 00:00:00
abstract::Three double-stranded RNA (dsRNA) segments from Aspergillus ochraceus isolate FA 0611, designated as AoR1, AoR2, and AoR3, were cloned and sequenced. AoR1 was identical with AoV dsRNA 1 previously reported from A. ochraceus ATCC 28706, which putatively encoded an RNA-dependent RNA polymerase of an unidentified mycovir...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-008-0265-6
更新日期:2008-12-01 00:00:00
abstract::Parvoviridae, which are classified into two subfamilies Parvovirinae and Densovirinae, can infect both vertebrate and insects and are related to a wide range of diseases in insects, animals, and humans. In this report, several new parvoviruses were identified in swine sera collected in southeastern China. The sequence...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-010-0506-3
更新日期:2010-10-01 00:00:00
abstract::We have determined the 5' and 3' non-coding regions (NCR) of four dengue-2 viruses isolated from dengue patients with different clinical severities in Nakhon Phanom, Northeastern Thailand in 1993. The results were compared to prototype dengue-2 strains and were found to have highest homology with the New Guinea C stra...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1007914520454
更新日期:1997-01-01 00:00:00
abstract::Serum samples collected from 88 Peruvians with unexplained fever were analyzed for viral sequences using metagenomics. Nucleic acids of anelloviruses, pegivirus A (GBV-C), HIV, Dengue virus, and Oropouche virus were detected. We also characterized from two sera the RNA genomes of new species of partitivirus and dicist...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-017-1514-3
更新日期:2018-02-01 00:00:00
abstract::Rice tungro spherical virus (RTSV) was shown to have three coat protein (CP) species by high resolution NaDodSO4-PAGE and Western blot analyses. The sequence of a coat protein-expressing cDNA clone that was identified and selected from a RTSV cDNA library showed that the insert was composed of 2823 bp with only one la...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF01702392
更新日期:1993-06-01 00:00:00
abstract::Coxsackieviruses are important human pathogens that induce myocarditis and pancreatitis. However, there are no vaccines or therapeutic reagents for their clinical treatment. Although RNA interference (RNAi)-based approaches to the prevention of viral production have been developed recently, limitations to the in vivo ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-007-0192-y
更新日期:2008-02-01 00:00:00
abstract::For millions of years viruses have adapted strategies to interfere with the immune defense of the host, which in turn has to deal with this challenge. In general the antiviral defense remains one step behind the pathogen. To achieve this strategic advantage large DNA-containing Viruses encode cellular homologues that ...
journal_title:Virus genes
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract::Two or three regions containing three or more successive newly defined heptads of a-d hydrophobic amino acid repeats have been located in the cDNA-derived amino acid sequences of glycoprotein G of all rhabdoviruses examined (rabies, vesicular stomatitis, fish, and plant rhabdoviruses) by computer search. These new hep...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF01702591
更新日期:1995-01-01 00:00:00
abstract::The NS3 protein of rice stripe virus (RSV), encoded by the virion strand of RNA3, is a viral suppressor of RNA silencing (VSR). Rice expressing NS3 had a normal phenotype, was initially sensitive to RSV but recovered at the later stages of infection. RSV accumulated slightly more in transgenic than in wild-type plants...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-014-1051-2
更新日期:2014-06-01 00:00:00
abstract::Genetic diversity of eight Grapevine leafroll-associated virus 1 (GLRaV-1) isolates recovered from grapevines in three distinct locations in the Czech Republic and Slovakia was characterised by restriction fragment length polymorphism (RFLP) analysis and by sequencing of cloned 540 bp fragment of the heat shock protei...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-005-3236-1
更新日期:2005-12-01 00:00:00
abstract::The complete genomic sequence of a sheep lentivirus isolate that presents a slow/low phenotype in vitro has been determined. The virus, designated P1OLV, was isolated from lung cells of a naturally infected sheep in Portugal. Three overlapping DNA fragments amplified by PCR, and encompassing the entire viral genome we...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/B:VIRU.0000036380.01957.37
更新日期:2004-10-01 00:00:00
abstract::Recently, we reported that the frequency of hepatitis C virus (HCV) genotypes and subtypes has rapidly changed among intravenous drug users (IDUs) in Yunnan Province over the last 5 years; this is especially true for subtype 6a which has increased in frequency from 5 to 15%. Here, we assessed 120 HCV-positive plasma s...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1420-0
更新日期:2017-04-01 00:00:00
abstract::Chinese equine infectious anemia virus (EIAV) attenuated vaccine is the first lentiviral vaccine with a successful application. In order to understand the correlation of viral genomic mutations with viral attenuation and with induced immunoprotective properties, we analyzed the proviral genome sequences of the EIAV-at...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-010-0491-6
更新日期:2010-08-01 00:00:00
abstract::The complete nucleotide sequences of neuraminidase (NA) of two swine influenza viruses (H1N1) are presented. A/Sw/Quebec/5393/91 (SwQc91) virus, associated with the chronic respiratory disease and A/Sw/ Quebec/192/81 (SwQc81) virus, associated with the acute respiratory disease, were used. The deduced amino acid seque...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1007944228967
更新日期:1997-01-01 00:00:00