Diversity and distribution of Maize-associated totivirus strains from Tanzania.

Abstract:

:Typically associated with fungal species, members of the viral family Totiviridae have recently been shown to be associated with plants, including important crop species, such as Carica papaya (papaya) and Zea mays (maize). Maize-associated totivirus (MATV) was first described in China and more recently in Ecuador, where it has been found to co-occur with other viruses known to elicit maize lethal necrosis disease (MLND). In a survey for maize-associated viruses, 35 samples were selected for Illumina HiSeq sequencing, from the Tanzanian maize producing regions of Mara, Arusha, Manyara, Kilimanjaro, Morogoro and Pwani. Libraries were prepared using an RNA-tag-seq methodology. Taxonomic classification of the resulting datasets showed that 6 of the 35 samples from the regions of Arusha, Kilimanjaro, Morogoro and Mara, contained reads that were assigned to MATV reference sequences. This was confirmed with PCR and Sanger sequencing. Read assembly of the six MATV-associated datasets yielded partial MATV genomes, two of which were selected for further characterization, using RACE. This yielded two full-length MATV genomes, one of which is divergent from other available MATV genomes.

journal_name

Virus Genes

journal_title

Virus genes

authors

Read DA,Featherston J,Rees DJG,Thompson GD,Roberts R,Flett BC,Mashingaidze K,Pietersen G,Kiula B,Kullaya A,Mbega ER

doi

10.1007/s11262-019-01650-6

subject

Has Abstract

pub_date

2019-06-01 00:00:00

pages

429-432

issue

3

eissn

0920-8569

issn

1572-994X

pii

10.1007/s11262-019-01650-6

journal_volume

55

pub_type

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