Comparison of multiple genes of spring viremia of carp viruses isolated in the United States.

Abstract:

:Five spring viremia of carp viruses (SVCV), Rhabdovirus carpio, were isolated in the United States (US) between 2002 and 2004. Single tube reverse transcription-polymerase chain reaction (RT-PCR) was used to generate overlapping cDNA fragments from the US isolates of SVCV. Multiple pairs of specific primers were designed to amplify a portion of the phosphoprotein gene, the matrix gene, and the glycoprotein gene of SVCV genogroup Id (corresponding to nucleotides 2174-4942 of GenBank accession NC_002803). Sequences were proofread and aligned to generate a consensus sequence for each isolate. Phylogenetic analysis of the 2705 nucleotide consensus sequence revealed that all five US isolates belong to SVCV genogroup Ia, Asian origin isolates, and a PCR primer binding site unique to SVCV genogroup Ia was identified.

journal_name

Virus Genes

journal_title

Virus genes

authors

Warg JV,Dikkeboom AL,Goodwin AE,Snekvik K,Whitney J

doi

10.1007/s11262-006-0042-3

subject

Has Abstract

pub_date

2007-08-01 00:00:00

pages

87-95

issue

1

eissn

0920-8569

issn

1572-994X

journal_volume

35

pub_type

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