Abstract:
:Five spring viremia of carp viruses (SVCV), Rhabdovirus carpio, were isolated in the United States (US) between 2002 and 2004. Single tube reverse transcription-polymerase chain reaction (RT-PCR) was used to generate overlapping cDNA fragments from the US isolates of SVCV. Multiple pairs of specific primers were designed to amplify a portion of the phosphoprotein gene, the matrix gene, and the glycoprotein gene of SVCV genogroup Id (corresponding to nucleotides 2174-4942 of GenBank accession NC_002803). Sequences were proofread and aligned to generate a consensus sequence for each isolate. Phylogenetic analysis of the 2705 nucleotide consensus sequence revealed that all five US isolates belong to SVCV genogroup Ia, Asian origin isolates, and a PCR primer binding site unique to SVCV genogroup Ia was identified.
journal_name
Virus Genesjournal_title
Virus genesauthors
Warg JV,Dikkeboom AL,Goodwin AE,Snekvik K,Whitney Jdoi
10.1007/s11262-006-0042-3subject
Has Abstractpub_date
2007-08-01 00:00:00pages
87-95issue
1eissn
0920-8569issn
1572-994Xjournal_volume
35pub_type
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