Nucleotide sequencing and serologic analysis of Cache Valley virus isolates from the Yucatan Peninsula of Mexico.

Abstract:

:Nucleotide sequencing was performed on part of the medium and large genome segments of 17 Cache Valley virus (CVV) isolates from the Yucatan Peninsula of Mexico. Alignment of these sequences to all other sequences in the Genbank database revealed that they have greatest nucleotide identity (97-98 %) with the equivalent regions of Tlacotalpan virus (TLAV), which is considered to be a variety of CVV. Next, cross-plaque reduction neutralization tests (PRNTs) were performed using sera from mice that had been inoculated with a representative isolate from the Yucatan Peninsula (CVV-478) or the prototype TLAV isolate (61-D-240). The PRNT titers exhibited a twofold difference in one direction and no difference in the other direction suggesting that CVV-478 and 61-D-240 belong to the same CVV subtype. In conclusion, we demonstrate that the CVV isolates from the Yucatan Peninsula of Mexico are genetically and antigenically similar to the prototype TLAV isolate.

journal_name

Virus Genes

journal_title

Virus genes

authors

Blitvich BJ,Loroño-Pino MA,Garcia-Rejon JE,Farfan-Ale JA,Dorman KS

doi

10.1007/s11262-012-0741-x

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

176-80

issue

1

eissn

0920-8569

issn

1572-994X

journal_volume

45

pub_type

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