Abstract:
:Nucleotide sequencing was performed on part of the medium and large genome segments of 17 Cache Valley virus (CVV) isolates from the Yucatan Peninsula of Mexico. Alignment of these sequences to all other sequences in the Genbank database revealed that they have greatest nucleotide identity (97-98 %) with the equivalent regions of Tlacotalpan virus (TLAV), which is considered to be a variety of CVV. Next, cross-plaque reduction neutralization tests (PRNTs) were performed using sera from mice that had been inoculated with a representative isolate from the Yucatan Peninsula (CVV-478) or the prototype TLAV isolate (61-D-240). The PRNT titers exhibited a twofold difference in one direction and no difference in the other direction suggesting that CVV-478 and 61-D-240 belong to the same CVV subtype. In conclusion, we demonstrate that the CVV isolates from the Yucatan Peninsula of Mexico are genetically and antigenically similar to the prototype TLAV isolate.
journal_name
Virus Genesjournal_title
Virus genesauthors
Blitvich BJ,Loroño-Pino MA,Garcia-Rejon JE,Farfan-Ale JA,Dorman KSdoi
10.1007/s11262-012-0741-xsubject
Has Abstractpub_date
2012-08-01 00:00:00pages
176-80issue
1eissn
0920-8569issn
1572-994Xjournal_volume
45pub_type
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