Phylogenetic analysis of partial RNA-polymerase blocks II and III of Rabies virus isolated from the main rabies reservoirs in Brazil.

Abstract:

:This study describes the results of the sequencing and analysis of segments of Blocks II and III of the RNA polymerase L gene of Rabies virus isolates from different reservoir species of Brazil. The phylogenetic relations of the virus were determined and a variety of species-specific nucleotides were found in the analyzed areas, but the majority of these mutations were found to be synonymous. However, an analysis of the putative amino acid sequences were shown to have some characteristic mutations between some reservoir species of Brazil, indicating that there was positive selection in the RNA polymerase L gene of Rabies virus. On comparing the putative viral sequences obtained from the Brazilian isolates and other Lyssavirus, it was determined that amino acid mutations occurred in low-restriction areas. This study of the L gene of Rabies virus is the first to be conducted with samples of virus isolates from Brazil, and the results obtained will help in the determination of the phylogenetic relations of the virus.

journal_name

Virus Genes

journal_title

Virus genes

authors

Carnieli P Jr,de Novaes Oliveira R,de Oliveira Fahl W,de Carvalho Ruthner Batista HB,Scheffer KC,Iamamoto K,Castilho JG

doi

10.1007/s11262-012-0743-8

subject

Has Abstract

pub_date

2012-08-01 00:00:00

pages

76-83

issue

1

eissn

0920-8569

issn

1572-994X

journal_volume

45

pub_type

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