Abstract:
:In our transient expression assays, the IE180 and TK promoters were markedly suppressed by the EP0 of the pseudorabies virus strains Ea and Fa (EP0/Ea, EP0/Fa). This is in contrast with the transactivating activity of EP0 from strain YS-81 (EP0/YS-81) in previous studies. Amino acid sequence alignment revealed several mutations in both EP0/Ea and EP0/Fa compared with EP0/YS-81. Most remarkable is a two-amino acid substitution next to the RING finger domain which was considered to be important for the transactivating activity of EP0 in previous studies. To address the effect of the two-amino acid substitution on the function of EP0, reverse mutants of EP0/Ea and EP0/Fa were generated. The subsequent expression assays indicated that this substitution was at least in part responsible for the effect on the regulatory activity of EP0. Our data suggested that EP0 may regulate the expression of the same genes in different pseudorabies virus strains differently.
journal_name
Virus Genesjournal_title
Virus genesauthors
Guo H,Zhou R,Xi Y,Xiao S,Chen Hdoi
10.1007/s11262-008-0320-3subject
Has Abstractpub_date
2009-04-01 00:00:00pages
269-75issue
2eissn
0920-8569issn
1572-994Xjournal_volume
38pub_type
杂志文章相关文献
VIRUS GENES文献大全abstract::We performed whole genome sequencing of 22 H9N2 avian influenza viruses (AIV) isolated from domestic laying hens on farms between 2005 and 2008, and compared the sequences with viruses previously reported in Asia. A previous study revealed that two antigenically distinct sublineages were established within the MS96 li...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-012-0737-6
更新日期:2012-08-01 00:00:00
abstract::Korean field infectious bursal disease viruses (IBDVs) were isolated from IBDV suspected commercial chickens. A previous study revealed that these IBDV field isolates were virulent or very virulent IBDVs. The isolates were passaged three times in the chorioallantoic membrane of specific-pathogen-free embryonated chick...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/b:viru.0000025776.52661.b0
更新日期:2004-04-01 00:00:00
abstract::This study focuses on gene expression of porcine circovirus (PCV) in order to identify viral genes and their corresponding mRNA transcripts. By northern blot analysis, the existence of three mRNAs could be demonstrated. Two mRNAs are encoded by the viral (-)-strand and one is encoded by the viral (+)-strand. The (+)-s...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008022521329
更新日期:1998-01-01 00:00:00
abstract::An evolutionary analysis was conducted of 354 hemagglutinin (HA) and 208 neuraminidase (NA) genes, including newly generated sequences of 5 HA and 30 NA, of Egyptian H5N1 clade 2.2.1 viruses isolated from poultry and humans. Five distinct phylogenetically distinguishable clusters arose from a monophyletic origin since...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-012-0758-1
更新日期:2012-08-01 00:00:00
abstract::Ramu stunt disease of sugarcane was first reported in Papua New Guinea in the mid 1980s. The disease can reduce sugarcane yields significantly and causes severe stunting and mortality in highly susceptible cultivars. The causal agent of Ramu stunt has been investigated but its characterization has not been completed. ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-015-1279-5
更新日期:2016-02-01 00:00:00
abstract::This study brings the analysis of amino acid sequences of hemagglutinin (HA) from the influenza virus A that can infect a wide variety of birds and mammals. 191 sequences belonging to all known 15 HA subtypes were compared. The emphasis was given on functional sites (receptor-binding cavity with its right and left edg...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1014038120979
更新日期:2002-01-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome (PRRS) is a swine disease of major economic importance that causes reproductive and respiratory problems in pigs. In the present study, one strain of porcine reproductive and respiratory syndrome virus (PRRSV) was isolated in Xinjiang province, Northwest China, designated ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-014-1122-4
更新日期:2015-02-01 00:00:00
abstract::Foot-and-mouth disease (FMD) is the most contagious and devastating disease of livestock. Our previous studies demonstrated that TK(-)/gG(-)/P1(+), a recombinant expressing the FMDV capsid precursor protein (P1) based on attenuated pseudorabies virus (PRV) TK(-)/gG(-), could be used as a recombinant vaccine to protect...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-008-0204-6
更新日期:2008-04-01 00:00:00
abstract::We describe the isolation and characterization of an insect-specific flavivirus (ISF) from Ochlerotatus caspius (Pallas, 1771) mosquitoes collected in southern Portugal. The RNA genome of this virus, tentatively designated OCFVPT, for O. caspius flavivirus from Portugal, encodes a polyprotein showing all the features ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-0960-9
更新日期:2013-12-01 00:00:00
abstract::Fourteen infectious bronchitis viruses (IBVs) were isolated in Korea between 2001 and 2003 from chickens suspected to be infected with IBVs. The nucleocapsid (N) protein genes of the various IBVs were amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and were cloned and sequenced, and the nucleotid...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-005-1788-8
更新日期:2005-10-01 00:00:00
abstract::Arenaviridae is a worldwide distributed family, of enveloped, single stranded, RNA viruses. The arenaviruses were divided in two major groups (Old World and New World), based on serological properties and genetic data, as well as the geographic distribution. In this study the phylogenetic relationship among the member...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1007993525052
更新日期:1998-01-01 00:00:00
abstract::The complete nucleotide sequences of neuraminidase (NA) of two swine influenza viruses (H1N1) are presented. A/Sw/Quebec/5393/91 (SwQc91) virus, associated with the chronic respiratory disease and A/Sw/ Quebec/192/81 (SwQc81) virus, associated with the acute respiratory disease, were used. The deduced amino acid seque...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1007944228967
更新日期:1997-01-01 00:00:00
abstract::Japanese encephalitis (JE), which is a mosquito-borne arboviral infection, is the leading cause of viral encephalitis in Asian countries. The causative agent of JE is Japanese encephalitis virus (JEV), in which the predominant genotype has changed from genotype III (G III) to genotype I (G I). However, a method for th...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-014-1158-5
更新日期:2015-04-01 00:00:00
abstract::The genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and similarity were mad...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00366986
更新日期:1996-01-01 00:00:00
abstract::Two ribozyme genes were designed to cut within the VP4 and VP3 sequences of foot and mouth disease virus (FMDV) Asia1 serotype genome. The two genes were synthesized and cloned into pBluescript under the control of the T3 promoter. The ribozyme designed to cut the VP4 gene contained two catalytic sequences targeted to...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF01702658
更新日期:1995-01-01 00:00:00
abstract::Human CMV (hCMV) encodes several captured chemokine ligand and chemokine receptor genes that may play a role in immune evasion. The adjacent viral alpha-chemokine genes UL146 and UL147 appear to have duplicated subsequent to a recent gene capture event. Sequence data from multiple hCMV isolates suggest accelerated pro...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-005-2201-3
更新日期:2005-08-01 00:00:00
abstract::Nucleotide sequencing was performed on part of the medium and large genome segments of 17 Cache Valley virus (CVV) isolates from the Yucatan Peninsula of Mexico. Alignment of these sequences to all other sequences in the Genbank database revealed that they have greatest nucleotide identity (97-98 %) with the equivalen...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-012-0741-x
更新日期:2012-08-01 00:00:00
abstract::The complete nucleotide sequence of a virus isolated from soybean (Glycine max (L.) Merr.) in China, previously identified as a new strain of Tobacco necrosis virus A (TNV-A) based on its biological, serological properties, and coat protein (CP) sequence and named as TNV-A C, was determined and compared with that of T...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-007-0185-x
更新日期:2008-02-01 00:00:00
abstract::We report here nucleotide sequence and characterization of gene 11 from two tissue culture-adapted ATCC rhesus (RRV) and human (Wa) strains of rotavirus. Gene 11 sequence encodes a nonstructural protein, NSP5 and also encodes NSP6, from an out of phase open reading frame. Sequence of RRV(ATCC) gene 11 represents the f...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1012577407824
更新日期:2001-12-01 00:00:00
abstract::The nucleotide sequence of two cloned restriction fragments encompassing the granulin genes from the granuloviruses of the potato tuber moth, Phthorimaea operculella, PhopGV, and the Egyptian cotton leaf worm, Spodoptera littoralis, SpliGV, have been determined. Although both viruses are able to infect the same Ph. op...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008179228236
更新日期:2000-10-01 00:00:00
abstract::A H3N2 canine influenza virus, A/canine/Guangdong/3/2011 (H3N2), was isolated from roaming dogs in rural China. Sequence and phylogenetic analysis of eight gene segments revealed that the A/canine/Guangdong/3/2011 (H3N2) was most similar to a recent H3N2 canine influenza virus isolated in cats from South Korea, which ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-0882-6
更新日期:2013-06-01 00:00:00
abstract::We previously identified a large homologous region (hr), hycu-hr6, in the genome of the Hyphantria cunea nucleopolyhedrovirus (HycuNPV) and suggested that hycu-hr6 was the largest baculovirus promoter enhancer hr identified so far. In this study, we examined the enhancement activity of hycu-hr6 against two promoters f...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0406-6
更新日期:2009-12-01 00:00:00
abstract::Mokola virus, one of the six genotypes within the Lyssavirus genus of the Rhabdoviridae family, is believed to be exclusive to the African continent, where infections in various mammal species have been reported. After an isolation of Mokola virus at Umhlanga on the east coast of South Africa in 1970, the virus was no...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008120511752
更新日期:2000-01-01 00:00:00
abstract::A low-cost rodent model of HIV infection and which presents high application value is an effective tool to investigate HIV infection and pathogenesis. However, development of such a small animal model has been hampered by the unsuitability of rodent cells for HIV-1 replication given that the retrovirus HIV-1 has high ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1423-x
更新日期:2017-04-01 00:00:00
abstract::The complete genomic sequence of canine calicivirus (CaCV) isolated from feces of a dog with diarrhea was determined. The CaCV genome, a positive-sense single-stranded RNA, contained 8513 nucleotides excluding the poly(A) tail and was longer than that of any other calicivirus strain with a completely known sequence. T...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1020174225622
更新日期:2002-01-01 00:00:00
abstract::A BK virus isolate cloned from the urine of an immunosuppressed patient was identified as the WW strain. Fine mapping using four base-pair restriction enzymes identified a number of small deletions and insertions relative to prototype BK. The finding of a transcriptional control region with a structure similar to that...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00315259
更新日期:1989-03-01 00:00:00
abstract::Brazil is one of the largest silk producers in the world. The domesticated silkworm (Bombyx mori) was formally introduced into the country in the twentieth century, and the state of Paraná is the main national producer. During larval stages, B. mori can be afflicted by many different infectious diseases, which lead to...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-014-1112-6
更新日期:2014-12-01 00:00:00
abstract::The VP2 gene DNA (1.4 kb in approximate) of a very virulent infectious bursal disease virus (vvIBDV) Chinju strain detected in Chinju, Korea was cloned into the bacmid, a baculovirus shuttle vector, through transposition of the gene from initially cloned pFastBacHTa plasmid, a baculovirus expression vector, and was su...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1025128619976
更新日期:2003-08-01 00:00:00
abstract::A 13 amino acid residue insertion was found in the N-terminal region of the coat protein of several Maize dwarf mosaic virus isolates (MDMV). These insertions seem to be the result of a direct duplication event, but differ in some positions. In order to evaluate the influence of the insertion on the RNA secondary stru...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0425-3
更新日期:2010-02-01 00:00:00
abstract::White spot syndrome virus (WSSV) is one of the major pathogens of cultured shrimp. Identification of envelope protein interactions has become a central issue for the understanding of WSSV assembly. In this paper, WSSV envelope protein VP52B was fused with GST-tag and expressed in Escherichia coli BL-21(DE3). Immunogol...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-014-1126-0
更新日期:2015-02-01 00:00:00