Abstract:
:Straw-colored fruit bats (Eidolon helvum), which have been identified as natural hosts for several zoonotic pathogens, such as lyssaviruses, henipaviruses, and ebolavirus, are associated with human settlements in Nigeria where they are commonly consumed as a delicacy. However, information on the viruses harbored by these bats is scarce. In this study, coronavirus sequences were detected using a nested RT-PCR targeting 440 bp of the RNA-dependent RNA polymerase (RdRp) in six of 79 fecal samples collected from an urban colony of E. helvum in Ibadan, Nigeria. Phylogenetic analysis revealed that all six sequences were monophyletic and clustered in lineage D of Betacoronavirus. The extension of two fragments allowed us to classify our sequences within the RdRp Group Unit defined for Kenyan Betacoronavirus from the same host species. These findings are consistent with the previous suggestion on the existence of a single epidemiological unit of E. helvum across sub-Saharan Africa. This theory, which is supported by the genetic structure of continental E. helvum, could facilitate viral mixing between different colonies across the continent.
journal_name
Virus Genesjournal_title
Virus genesauthors
Leopardi S,Oluwayelu D,Meseko C,Marciano S,Tassoni L,Bakarey S,Monne I,Cattoli G,De Benedictis Pdoi
10.1007/s11262-016-1331-0subject
Has Abstractpub_date
2016-08-01 00:00:00pages
573-7issue
4eissn
0920-8569issn
1572-994Xpii
10.1007/s11262-016-1331-0journal_volume
52pub_type
杂志文章相关文献
VIRUS GENES文献大全abstract::The S2 glycoprotein and membrane (M) protein genes and S1 glycoprotein and nucleocapsid (N) genes of 11 Korean infectious bronchitis virus (IBV) isolates were amplified by RT-PCR, cloned, and sequenced. The resultant nucleotide sequences were compared with the published sequences for non-Korean IBV strains. Korean IBV...
journal_title:Virus genes
pub_type: 信件
doi:10.1007/s11262-012-0856-0
更新日期:2013-04-01 00:00:00
abstract::The complete genome sequence of Nootka lupine vein-clearing virus (NLVCV) was determined to be 4,172 nucleotides in length containing four open reading frames (ORFs) with a similar genetic organization of virus species in the genus Carmovirus, family Tombusviridae. The order and gene product size, starting from the 5'...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-007-0139-3
更新日期:2007-12-01 00:00:00
abstract::The Kansas/15626/84 (Ka) and Shipping Fever (SF) strains of bovine parainfluenza virus type 3 (BPIV3) replicate less efficiently than human PIV3 (HPIV3) in the upper and lower respiratory tract of rhesus monkeys, and BPIV3 Ka is also highly attenuated in humans and is in clinical trials as a candidate vaccine against ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008130917204
更新日期:2000-01-01 00:00:00
abstract::E6 and E7 genes of human papilloma virus type 18 have been subcloned from plasmid pC7, carrying an insert of DNA from squamous cell carcinoma of cervix. Both genes in comparison to prototype variant contain one mutation that changes asparagine to leucine. In the case of E6 gene this mutation is mapped in codon 129, in...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008020719309
更新日期:1999-01-01 00:00:00
abstract::We have determined the 5' and 3' non-coding regions (NCR) of four dengue-2 viruses isolated from dengue patients with different clinical severities in Nakhon Phanom, Northeastern Thailand in 1993. The results were compared to prototype dengue-2 strains and were found to have highest homology with the New Guinea C stra...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1007914520454
更新日期:1997-01-01 00:00:00
abstract::Rice tungro spherical virus (RTSV) was shown to have three coat protein (CP) species by high resolution NaDodSO4-PAGE and Western blot analyses. The sequence of a coat protein-expressing cDNA clone that was identified and selected from a RTSV cDNA library showed that the insert was composed of 2823 bp with only one la...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF01702392
更新日期:1993-06-01 00:00:00
abstract::We performed whole genome sequencing of 22 H9N2 avian influenza viruses (AIV) isolated from domestic laying hens on farms between 2005 and 2008, and compared the sequences with viruses previously reported in Asia. A previous study revealed that two antigenically distinct sublineages were established within the MS96 li...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-012-0737-6
更新日期:2012-08-01 00:00:00
abstract::This study brings the analysis of amino acid sequences of hemagglutinin (HA) from the influenza virus A that can infect a wide variety of birds and mammals. 191 sequences belonging to all known 15 HA subtypes were compared. The emphasis was given on functional sites (receptor-binding cavity with its right and left edg...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1014038120979
更新日期:2002-01-01 00:00:00
abstract::The complete nucleotide sequences of neuraminidase (NA) of two swine influenza viruses (H1N1) are presented. A/Sw/Quebec/5393/91 (SwQc91) virus, associated with the chronic respiratory disease and A/Sw/ Quebec/192/81 (SwQc81) virus, associated with the acute respiratory disease, were used. The deduced amino acid seque...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1007944228967
更新日期:1997-01-01 00:00:00
abstract::Ramu stunt disease of sugarcane was first reported in Papua New Guinea in the mid 1980s. The disease can reduce sugarcane yields significantly and causes severe stunting and mortality in highly susceptible cultivars. The causal agent of Ramu stunt has been investigated but its characterization has not been completed. ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-015-1279-5
更新日期:2016-02-01 00:00:00
abstract::A unique feature of ascovirus infection is cleavage of host cells into virus containing vesicles. It has been suggested that the virus induces apoptosis, either by expression of a caspase or other means, which is then diverted toward vesicle formation. There is little known about the mechanism of vesicle formation. Re...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-009-0407-5
更新日期:2009-12-01 00:00:00
abstract::The infection of humans with Boma disease virus (BDV) is still a matter of debate. In a recent publication, we described a BDV (RW98) isolated from the blood of a psychiatric patient. The RNA of this virus differed more than 5% from that of the widely used strain He/80, which was supposed to represent our laboratory v...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1022321920287
更新日期:2003-01-01 00:00:00
abstract::Novel duck reovirus (NDRV), the prototype strain of the species Avian orthoreovirus (ARV), is associated with high mortality in Pekin ducklings. σC is an outer capsid protein encoded by the S1 genome segment of NDRV which mediates attachment to host cells. Our previous studies using immunoprecipitation and mass spectr...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-020-01750-8
更新日期:2020-06-01 00:00:00
abstract::African monkeys are resistant to HIV-1 infection due to intrinsic restriction mechanisms found in their cells. However, although they can be infected by monkey-adapted modified HIV-1 particles that are designed to overcome known restriction factors, virus numbers drop to undetectable levels in immunocompetent animals....
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-020-01731-x
更新日期:2020-04-01 00:00:00
abstract::Nine avian influenza viruses (AIV), H5N1 subtype, were isolated from dead poultry in the Karachi region of Pakistan from 2006 to 2008. The intravenous pathogenicity indices and HA protein cleavage sites of all nine viruses were consistent with highly pathogenic AIV. Based on phylogenetic analysis of the HA genes, thes...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-011-0689-2
更新日期:2012-04-01 00:00:00
abstract::Recently, NADC30-like porcine reproductive and respiratory syndrome viruses (PRRSVs), which are genetically similar to the NADC30 strain isolated in the United States of America in 2008, have become prevalent in China. Here, a novel variant PRRSV strain named HNhx was successfully isolated on porcine alveolar macropha...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-017-1516-1
更新日期:2018-02-01 00:00:00
abstract::There are eight genotypes A-H of hepatitis B virus (HBV). Most genotypes are further divided into subgenotypes. Genotypes and subgenotypes influence the natural course of infection and therapy. We analysed nine sera from HBV carriers from Peru. Using the small hepatitis B surface protein HBs, all samples could be grou...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-008-0261-x
更新日期:2008-10-01 00:00:00
abstract::The genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and similarity were mad...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00366986
更新日期:1996-01-01 00:00:00
abstract::Nitric oxide (NO) has been shown to suppress Japanese encephalitis virus (JEV) RNA synthesis, viral protein accumulation, and virus release from infected cells. In this article, the potential viral structural proteins as the activators of NO product were studied at the molecular level. First, the genomic region encodi...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-008-0299-9
更新日期:2009-02-01 00:00:00
abstract::Cap-independent translations of viral RNAs of enteroviruses and rhinoviruses, cardioviruses and aphthoviruses, hepatitis A and C viruses (HAV and HCV), and pestivirus are initiated by the direct binding of 40S ribosomal subunits to a cis-acting genetic element termed the internal ribosome entry site (IRES) or ribosome...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/BF00572952
更新日期:1996-01-01 00:00:00
abstract::We report here the complete genome sequence of a bipartite virus, herein denoted WLPRV/human/BRA/TO-34/201, from a sample collected in 2015 from a two-year-old child in Brazil presenting acute gastroenteritis. The virus has 98-99% identity (segments 2 and 1, respectively) with the Wuhan large pig roundworm virus (uncl...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-018-1557-0
更新日期:2018-06-01 00:00:00
abstract::Cotton leaf curl disease (CLCuD) has been a major constraint to cotton production across Pakistan and northwestern India since the early 1990s. The disease is caused by a number of begomoviruses, including Cotton leaf curl Multan virus (CLCuMuV), which associate with a specific host range and symptom determining betas...
journal_title:Virus genes
pub_type: 信件
doi:10.1007/s11262-018-1605-9
更新日期:2018-12-01 00:00:00
abstract::Herpes simplex virus type 1 (HSV-1) reactivates from a small fraction of latently infected neurons in vivo and neuronally differentiated (ND), quiescently infected (QIF)-PC12 cells in vitro. This may be the result of reactivation initiating in only a few cells, or reactivation followed by premature termination of the ...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-005-0052-6
更新日期:2006-10-01 00:00:00
abstract::Kenya is one of the high endemic zones for hepatitis B virus (HBV) infection. The consensuses on prevalence of the HBV genotypes and the existence of their variants have not been fully established in Kenya. Hence, there is a need to further monitor the diversity of HBV. This study aimed to extend the current molecular...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-0976-1
更新日期:2013-12-01 00:00:00
abstract::Two restriction fragments of bovine papillomavirus type 5 (BPV5), of a genomic size of 1.6 and 1.2 kb were subcloned and sequenced. One of them seemed to correspond to the 3' end of the E1 open reading frame (ORF) and the other to the region of the E7, E8 and 5' end of the E1 ORF. Alignments of these fragments with ot...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1007960324424
更新日期:1997-01-01 00:00:00
abstract::Merkel cell carcinoma (MCC) is a highly aggressive neuroendocrine skin cancer associated with the Merkel cell polyomavirus (MCPyV). The MCPyV genome, which is clonally integrated in the majority of MCCs, encodes the regulatory small T (sT) antigen. Previously, reports have established MCPyV sT antigen as a potent onco...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-016-1304-3
更新日期:2016-06-01 00:00:00
abstract::The complete genomic sequence of a sheep lentivirus isolate that presents a slow/low phenotype in vitro has been determined. The virus, designated P1OLV, was isolated from lung cells of a naturally infected sheep in Portugal. Three overlapping DNA fragments amplified by PCR, and encompassing the entire viral genome we...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/B:VIRU.0000036380.01957.37
更新日期:2004-10-01 00:00:00
abstract::Glycosylation on the globular head of the hemagglutinin (HA) protein of influenza virus acts as an important target for recognition and destruction of virus by innate immune proteins of the collectin family. In the current study, we have characterized the dynamic amino acid changes at N-linked glycosylation sites of f...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-013-0993-0
更新日期:2014-02-01 00:00:00
abstract::Sacbrood virus (SBV) represents a serious threat to the health of managed honeybees. We determined four complete SBV genomic sequences (AmSBV-Kor1, AmSBV-Kor2, AcSBV-Kor3, and AcSBV-Kor4) isolated from Apis mellifera and Apis cerana in various regions of South Korea. A phylogenetic tree was constructed from the comple...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1007/s11262-015-1268-8
更新日期:2016-04-01 00:00:00
abstract::Mokola virus, one of the six genotypes within the Lyssavirus genus of the Rhabdoviridae family, is believed to be exclusive to the African continent, where infections in various mammal species have been reported. After an isolation of Mokola virus at Umhlanga on the east coast of South Africa in 1970, the virus was no...
journal_title:Virus genes
pub_type: 杂志文章
doi:10.1023/a:1008120511752
更新日期:2000-01-01 00:00:00