The close genetic relationship of lineage D Betacoronavirus from Nigerian and Kenyan straw-colored fruit bats (Eidolon helvum) is consistent with the existence of a single epidemiological unit across sub-Saharan Africa.

Abstract:

:Straw-colored fruit bats (Eidolon helvum), which have been identified as natural hosts for several zoonotic pathogens, such as lyssaviruses, henipaviruses, and ebolavirus, are associated with human settlements in Nigeria where they are commonly consumed as a delicacy. However, information on the viruses harbored by these bats is scarce. In this study, coronavirus sequences were detected using a nested RT-PCR targeting 440 bp of the RNA-dependent RNA polymerase (RdRp) in six of 79 fecal samples collected from an urban colony of E. helvum in Ibadan, Nigeria. Phylogenetic analysis revealed that all six sequences were monophyletic and clustered in lineage D of Betacoronavirus. The extension of two fragments allowed us to classify our sequences within the RdRp Group Unit defined for Kenyan Betacoronavirus from the same host species. These findings are consistent with the previous suggestion on the existence of a single epidemiological unit of E. helvum across sub-Saharan Africa. This theory, which is supported by the genetic structure of continental E. helvum, could facilitate viral mixing between different colonies across the continent.

journal_name

Virus Genes

journal_title

Virus genes

authors

Leopardi S,Oluwayelu D,Meseko C,Marciano S,Tassoni L,Bakarey S,Monne I,Cattoli G,De Benedictis P

doi

10.1007/s11262-016-1331-0

subject

Has Abstract

pub_date

2016-08-01 00:00:00

pages

573-7

issue

4

eissn

0920-8569

issn

1572-994X

pii

10.1007/s11262-016-1331-0

journal_volume

52

pub_type

杂志文章
  • Sequencing, phylogenetic analysis, and potential recombination events of infectious bronchitis viruses isolated in Korea.

    abstract::The S2 glycoprotein and membrane (M) protein genes and S1 glycoprotein and nucleocapsid (N) genes of 11 Korean infectious bronchitis virus (IBV) isolates were amplified by RT-PCR, cloned, and sequenced. The resultant nucleotide sequences were compared with the published sequences for non-Korean IBV strains. Korean IBV...

    journal_title:Virus genes

    pub_type: 信件

    doi:10.1007/s11262-012-0856-0

    authors: Song JE,Jeong WG,Sung HW,Kwon HM

    更新日期:2013-04-01 00:00:00

  • Complete nucleotide sequence of Nootka lupine vein-clearing virus.

    abstract::The complete genome sequence of Nootka lupine vein-clearing virus (NLVCV) was determined to be 4,172 nucleotides in length containing four open reading frames (ORFs) with a similar genetic organization of virus species in the genus Carmovirus, family Tombusviridae. The order and gene product size, starting from the 5'...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-007-0139-3

    authors: Robertson NL,Côté F,Paré C,Leblanc E,Bergeron MG,Leclerc D

    更新日期:2007-12-01 00:00:00

  • Sequence determination and molecular analysis of two strains of bovine parainfluenza virus type 3 that are attenuated for primates.

    abstract::The Kansas/15626/84 (Ka) and Shipping Fever (SF) strains of bovine parainfluenza virus type 3 (BPIV3) replicate less efficiently than human PIV3 (HPIV3) in the upper and lower respiratory tract of rhesus monkeys, and BPIV3 Ka is also highly attenuated in humans and is in clinical trials as a candidate vaccine against ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1008130917204

    authors: Bailly JE,McAuliffe JM,Skiadopoulos MH,Collins PL,Murphy BR

    更新日期:2000-01-01 00:00:00

  • Cloning of E6 and E7 genes of human papilloma virus type 18 and transformation potential of E7 gene and its mutants.

    abstract::E6 and E7 genes of human papilloma virus type 18 have been subcloned from plasmid pC7, carrying an insert of DNA from squamous cell carcinoma of cervix. Both genes in comparison to prototype variant contain one mutation that changes asparagine to leucine. In the case of E6 gene this mutation is mapped in codon 129, in...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1008020719309

    authors: Laassri M,Gul'ko L,Vinokurova S,Kisseljova N,Veiko V,Kisseljov F

    更新日期:1999-01-01 00:00:00

  • Sequences of terminal non-coding regions from four dengue-2 viruses isolated from patients exhibiting different disease severities.

    abstract::We have determined the 5' and 3' non-coding regions (NCR) of four dengue-2 viruses isolated from dengue patients with different clinical severities in Nakhon Phanom, Northeastern Thailand in 1993. The results were compared to prototype dengue-2 strains and were found to have highest homology with the New Guinea C stra...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1007914520454

    authors: Mangada MN,Igarashi A

    更新日期:1997-01-01 00:00:00

  • Molecular cloning and sequencing of coat protein-encoding cDNA of rice tungro spherical virus--a plant picornavirus.

    abstract::Rice tungro spherical virus (RTSV) was shown to have three coat protein (CP) species by high resolution NaDodSO4-PAGE and Western blot analyses. The sequence of a coat protein-expressing cDNA clone that was identified and selected from a RTSV cDNA library showed that the insert was composed of 2823 bp with only one la...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF01702392

    authors: Zhang S,Jones MC,Barker P,Davies JW,Hull R

    更新日期:1993-06-01 00:00:00

  • Genetic evolution of the H9N2 avian influenza virus in Korean poultry farms.

    abstract::We performed whole genome sequencing of 22 H9N2 avian influenza viruses (AIV) isolated from domestic laying hens on farms between 2005 and 2008, and compared the sequences with viruses previously reported in Asia. A previous study revealed that two antigenically distinct sublineages were established within the MS96 li...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-012-0737-6

    authors: Lee CH,Byun SH,Lee YJ,Mo IP

    更新日期:2012-08-01 00:00:00

  • Sequence similarities and evolutionary relationships of influenza virus A hemagglutinins.

    abstract::This study brings the analysis of amino acid sequences of hemagglutinin (HA) from the influenza virus A that can infect a wide variety of birds and mammals. 191 sequences belonging to all known 15 HA subtypes were compared. The emphasis was given on functional sites (receptor-binding cavity with its right and left edg...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1014038120979

    authors: Kovácová A,Ruttkay-Nedecký G,Haverlík IK,Janecek S

    更新日期:2002-01-01 00:00:00

  • Complete sequences of the neuraminidase genes of swine influenza viruses (H1N1) associated with the respiratory disease in pigs.

    abstract::The complete nucleotide sequences of neuraminidase (NA) of two swine influenza viruses (H1N1) are presented. A/Sw/Quebec/5393/91 (SwQc91) virus, associated with the chronic respiratory disease and A/Sw/ Quebec/192/81 (SwQc81) virus, associated with the acute respiratory disease, were used. The deduced amino acid seque...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1007944228967

    authors: Arora DS,Tijssen P,Dea S,Henrichon M

    更新日期:1997-01-01 00:00:00

  • Detection by next generation sequencing of a multi-segmented viral genome from sugarcane associated with Ramu stunt disease.

    abstract::Ramu stunt disease of sugarcane was first reported in Papua New Guinea in the mid 1980s. The disease can reduce sugarcane yields significantly and causes severe stunting and mortality in highly susceptible cultivars. The causal agent of Ramu stunt has been investigated but its characterization has not been completed. ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-015-1279-5

    authors: Mollov D,Maroon-Lango C,Kuniata L

    更新日期:2016-02-01 00:00:00

  • A lipase-like gene from Heliothis virescens ascovirus (HvAV-3e) is essential for virus replication and cell cleavage.

    abstract::A unique feature of ascovirus infection is cleavage of host cells into virus containing vesicles. It has been suggested that the virus induces apoptosis, either by expression of a caspase or other means, which is then diverted toward vesicle formation. There is little known about the mechanism of vesicle formation. Re...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-009-0407-5

    authors: Smede M,Hussain M,Asgari S

    更新日期:2009-12-01 00:00:00

  • Genetic relationship of Borna disease virus isolates.

    abstract::The infection of humans with Boma disease virus (BDV) is still a matter of debate. In a recent publication, we described a BDV (RW98) isolated from the blood of a psychiatric patient. The RNA of this virus differed more than 5% from that of the widely used strain He/80, which was supposed to represent our laboratory v...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1022321920287

    authors: Planz O,Rziha HJ,Stitz L

    更新日期:2003-01-01 00:00:00

  • Interaction between Translocation-associated membrane protein 1 and σC protein of novel duck reovirus controls virus infectivity.

    abstract::Novel duck reovirus (NDRV), the prototype strain of the species Avian orthoreovirus (ARV), is associated with high mortality in Pekin ducklings. σC is an outer capsid protein encoded by the S1 genome segment of NDRV which mediates attachment to host cells. Our previous studies using immunoprecipitation and mass spectr...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-020-01750-8

    authors: Xiao R,Mi X,Sun J,Ding M,Li C,Zhu J,Liu G,Ma W,Zhou H,Chen Z

    更新日期:2020-06-01 00:00:00

  • Human immunodeficiency virus type-1 Tat protein induces secretory leukocyte protease inhibitor expression in African green monkey but not human cells.

    abstract::African monkeys are resistant to HIV-1 infection due to intrinsic restriction mechanisms found in their cells. However, although they can be infected by monkey-adapted modified HIV-1 particles that are designed to overcome known restriction factors, virus numbers drop to undetectable levels in immunocompetent animals....

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-020-01731-x

    authors: Özdemir S,Şengez B,Arslanoğlu A

    更新日期:2020-04-01 00:00:00

  • Characterization of H5N1 highly pathogenic avian influenza viruses isolated from poultry in Pakistan 2006-2008.

    abstract::Nine avian influenza viruses (AIV), H5N1 subtype, were isolated from dead poultry in the Karachi region of Pakistan from 2006 to 2008. The intravenous pathogenicity indices and HA protein cleavage sites of all nine viruses were consistent with highly pathogenic AIV. Based on phylogenetic analysis of the HA genes, thes...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-011-0689-2

    authors: Wasilenko JL,Pantin-Jackwood M,Khan TA,Ahmed A,Rehmani SF,Lone NA,Swayne DE,Spackman E

    更新日期:2012-04-01 00:00:00

  • Genomic analysis of a recombinant NADC30-like porcine reproductive and respiratory syndrome virus in china.

    abstract::Recently, NADC30-like porcine reproductive and respiratory syndrome viruses (PRRSVs), which are genetically similar to the NADC30 strain isolated in the United States of America in 2008, have become prevalent in China. Here, a novel variant PRRSV strain named HNhx was successfully isolated on porcine alveolar macropha...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-017-1516-1

    authors: Wang LJ,Wan B,Guo Z,Qiao S,Li R,Xie S,Chen XX,Zhang G

    更新日期:2018-02-01 00:00:00

  • A new clade of hepatitis B virus subgenotype F1 from Peru with unusual properties.

    abstract::There are eight genotypes A-H of hepatitis B virus (HBV). Most genotypes are further divided into subgenotypes. Genotypes and subgenotypes influence the natural course of infection and therapy. We analysed nine sera from HBV carriers from Peru. Using the small hepatitis B surface protein HBs, all samples could be grou...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-008-0261-x

    authors: von Meltzer M,Vásquez S,Sun J,Wendt UC,May A,Gerlich WH,Radtke M,Schaefer S

    更新日期:2008-10-01 00:00:00

  • Clustering of Argentinean tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene.

    abstract::The genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and similarity were mad...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF00366986

    authors: Dewey R,Semorille L,Crisci J,Grau O

    更新日期:1996-01-01 00:00:00

  • Recombinant core proteins of Japanese encephalitis virus as activators of the innate immune response.

    abstract::Nitric oxide (NO) has been shown to suppress Japanese encephalitis virus (JEV) RNA synthesis, viral protein accumulation, and virus release from infected cells. In this article, the potential viral structural proteins as the activators of NO product were studied at the molecular level. First, the genomic region encodi...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-008-0299-9

    authors: Chen SO,Fang SH,Shih DY,Chang TJ,Liu JJ

    更新日期:2009-02-01 00:00:00

  • A common structural core in the internal ribosome entry sites of picornavirus, hepatitis C virus, and pestivirus.

    abstract::Cap-independent translations of viral RNAs of enteroviruses and rhinoviruses, cardioviruses and aphthoviruses, hepatitis A and C viruses (HAV and HCV), and pestivirus are initiated by the direct binding of 40S ribosomal subunits to a cis-acting genetic element termed the internal ribosome entry site (IRES) or ribosome...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/BF00572952

    authors: Le SY,Siddiqui A,Maizel JV Jr

    更新日期:1996-01-01 00:00:00

  • Wuhan large pig roundworm virus identified in human feces in Brazil.

    abstract::We report here the complete genome sequence of a bipartite virus, herein denoted WLPRV/human/BRA/TO-34/201, from a sample collected in 2015 from a two-year-old child in Brazil presenting acute gastroenteritis. The virus has 98-99% identity (segments 2 and 1, respectively) with the Wuhan large pig roundworm virus (uncl...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-018-1557-0

    authors: Luchs A,Leal E,Komninakis SV,de Pádua Milagres FA,Brustulin R,da Aparecida Rodrigues Teles M,Gill DE,Deng X,Delwart E,Sabino EC,da Costa AC

    更新日期:2018-06-01 00:00:00

  • Transmission of cotton leaf curl disease: answer to a long-standing question.

    abstract::Cotton leaf curl disease (CLCuD) has been a major constraint to cotton production across Pakistan and northwestern India since the early 1990s. The disease is caused by a number of begomoviruses, including Cotton leaf curl Multan virus (CLCuMuV), which associate with a specific host range and symptom determining betas...

    journal_title:Virus genes

    pub_type: 信件

    doi:10.1007/s11262-018-1605-9

    authors: Masood M,Briddon RW

    更新日期:2018-12-01 00:00:00

  • Reactivation from quiescence does not coincide with a global induction of herpes simplex virus type 1 transactivators.

    abstract::Herpes simplex virus type 1 (HSV-1) reactivates from a small fraction of latently infected neurons in vivo and neuronally differentiated (ND), quiescently infected (QIF)-PC12 cells in vitro. This may be the result of reactivation initiating in only a few cells, or reactivation followed by premature termination of the ...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-005-0052-6

    authors: Danaher RJ,Jacob RJ,Miller CS

    更新日期:2006-10-01 00:00:00

  • Hepatitis B virus subgenotype A1, occurrence of subgenotype D4, and S gene mutations among voluntary blood donors in Kenya.

    abstract::Kenya is one of the high endemic zones for hepatitis B virus (HBV) infection. The consensuses on prevalence of the HBV genotypes and the existence of their variants have not been fully established in Kenya. Hence, there is a need to further monitor the diversity of HBV. This study aimed to extend the current molecular...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-0976-1

    authors: Kwange SO,Budambula NL,Kiptoo MK,Okoth F,Ochwoto M,Oduor M,Kimotho JH

    更新日期:2013-12-01 00:00:00

  • Bovine papillomavirus type 5: partial sequence and comparison with other bovine papillomaviruses.

    abstract::Two restriction fragments of bovine papillomavirus type 5 (BPV5), of a genomic size of 1.6 and 1.2 kb were subcloned and sequenced. One of them seemed to correspond to the 3' end of the E1 open reading frame (ORF) and the other to the region of the E7, E8 and 5' end of the E1 ORF. Alignments of these fragments with ot...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1007960324424

    authors: Bloch N,Breen M

    更新日期:1997-01-01 00:00:00

  • Merkel cell polyomavirus in Merkel cell carcinogenesis: small T antigen-mediates c-Jun phosphorylation.

    abstract::Merkel cell carcinoma (MCC) is a highly aggressive neuroendocrine skin cancer associated with the Merkel cell polyomavirus (MCPyV). The MCPyV genome, which is clonally integrated in the majority of MCCs, encodes the regulatory small T (sT) antigen. Previously, reports have established MCPyV sT antigen as a potent onco...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-016-1304-3

    authors: Wu JH,Simonette RA,Nguyen HP,Rady PL,Tyring SK

    更新日期:2016-06-01 00:00:00

  • Genomic characterization of a slow/low maedi visna virus.

    abstract::The complete genomic sequence of a sheep lentivirus isolate that presents a slow/low phenotype in vitro has been determined. The virus, designated P1OLV, was isolated from lung cells of a naturally infected sheep in Portugal. Three overlapping DNA fragments amplified by PCR, and encompassing the entire viral genome we...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/B:VIRU.0000036380.01957.37

    authors: Barros SC,Ramos F,Duarte M,Fagulha T,Cruz B,Fevereiro M

    更新日期:2004-10-01 00:00:00

  • Loss and gain of N-linked glycosylation sites in globular head and stem of HA found in A/H3N2 flu fatal and severe cases during 2013 Tunisia flu seasonal survey.

    abstract::Glycosylation on the globular head of the hemagglutinin (HA) protein of influenza virus acts as an important target for recognition and destruction of virus by innate immune proteins of the collectin family. In the current study, we have characterized the dynamic amino acid changes at N-linked glycosylation sites of f...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-013-0993-0

    authors: El Moussi A,Ben Hadj Kacem MA,Slim A

    更新日期:2014-02-01 00:00:00

  • Homology differences between complete Sacbrood virus genomes from infected Apis mellifera and Apis cerana honeybees in Korea.

    abstract::Sacbrood virus (SBV) represents a serious threat to the health of managed honeybees. We determined four complete SBV genomic sequences (AmSBV-Kor1, AmSBV-Kor2, AcSBV-Kor3, and AcSBV-Kor4) isolated from Apis mellifera and Apis cerana in various regions of South Korea. A phylogenetic tree was constructed from the comple...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1007/s11262-015-1268-8

    authors: Reddy KE,Yoo MS,Kim YH,Kim NH,Ramya M,Jung HN,Thao le TB,Lee HS,Kang SW

    更新日期:2016-04-01 00:00:00

  • New cases of Mokola virus infection in South Africa: a genotypic comparison of Southern African virus isolates.

    abstract::Mokola virus, one of the six genotypes within the Lyssavirus genus of the Rhabdoviridae family, is believed to be exclusive to the African continent, where infections in various mammal species have been reported. After an isolation of Mokola virus at Umhlanga on the east coast of South Africa in 1970, the virus was no...

    journal_title:Virus genes

    pub_type: 杂志文章

    doi:10.1023/a:1008120511752

    authors: Nel L,Jacobs J,Jaftha J,von Teichman B,Bingham J,Olivier M

    更新日期:2000-01-01 00:00:00