Abstract：
:Rabies is a lethal viral infection that can affect almost all mammals, including humans. To better understand the replication of Rabies lyssavirus, we investigated if the viral load in brains naturally infected with rabies influences viral internalization and viral growth kinetics in neuroblastoma cells, and if the viral load affects mortality in mice after intradermal infection. We noted that high initial viral loads in brains (group II) were unfavourable for increasing viral titers during serial passages in neuroblastoma cells when compared to low initial viral loads in brains (group I). In addition, group I strains showed higher viral growth and enhanced internalization efficiency in neuroblastoma cells than group II strains. However, we observed that the dominant virus subpopulation in group II promoted efficient viral infection in the central nervous system in the new host, providing a selective advantage to the virus. Our data indicate that rabies infection in animal models depends on not only the virus strain but also the amount of virus. This study may serve as a basis for understanding the biologic proprieties of Rabies lyssavirus strains with respect to the effects on viral replication and the impact on pathogenesis, improving virus yields for use in vaccine development.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Fuoco NL,Dos Ramos Silva S,Fernandes ER,Luiz FG,Ribeiro OG,Katz ISSdoi
10.1016/j.antiviral.2017.11.003subject
Has Abstractpub_date
2018-01-01 00:00:00pages
89-94eissn
0166-3542issn
1872-9096pii
S0166-3542(17)30614-9journal_volume
149pub_type
杂志文章abstract：:For the routine manufacture of live virus vaccines, virus is diluted into a formulation buffer to stabilize it for long-term storage, and to facilitate vaccine administration. The characteristics of this buffer are dependent on the storage temperature of the vaccine, as well as the desired characteristics of the produ...
journal_title：Antiviral research
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journal_title：Antiviral research
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pub_type： 杂志文章
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更新日期：2012-06-01 00:00:00
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journal_title：Antiviral research
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journal_title：Antiviral research
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pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104905
更新日期：2020-10-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
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更新日期：2019-10-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104974
更新日期：2021-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.08.020
更新日期：2016-10-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2005.05.001
更新日期：2005-08-01 00:00:00
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doi：10.1016/j.antiviral.2014.10.005
更新日期：2014-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104867
更新日期：2020-11-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2013.08.024
更新日期：2013-11-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2013.05.013
更新日期：2013-08-01 00:00:00
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journal_title：Antiviral research
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doi：10.1016/j.antiviral.2012.02.001
更新日期：2012-04-01 00:00:00
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doi：10.1016/j.antiviral.2004.08.002
更新日期：2004-12-01 00:00:00
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更新日期：1996-05-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2009.09.010
更新日期：2009-12-01 00:00:00
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更新日期：2016-02-01 00:00:00
abstract：:Many flaviviruses, such as Zika virus (ZIKV), Dengue virus (DENV1-4) and yellow fever virus (YFV), are significant human pathogens. Infection with ZIKV, an emerging mosquito-borne flavivirus, is associated with increased risk of microcephaly in newborns and Guillain-Barré syndrome and other complications in adults. Cu...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2017.12.018
更新日期：2018-02-01 00:00:00
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更新日期：1984-10-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2014.05.018
更新日期：2014-08-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(92)90038-7
更新日期：1992-06-01 00:00:00
abstract：:Interferon alpha-2b (IFN) alone or in combination with Ribavirin is approved in the United States for the treatment of chronic hepatitis C virus (HCV) infection. We have previously reported that the glucosidase inhibitor, n-butyl deoxynojirimycin (nB-DNJ) inhibits the production of infectious bovine diarrhea virus (BV...
journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2002-09-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(90)90031-2
更新日期：1990-09-01 00:00:00
