Abstract：
:Foot and mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. Previously, we found that the epitope peptide EP141-160 displayed on virus-like particles (VLP) for use as a vaccine showed high immunoreactivity and conferred partially effective protection to animals. In this study, we first combined antisense RNA with VLP as a vaccine against the foot-and-mouth disease virus (FMDV) by using a prokaryotic co-expression system. The antisense RNA against the 3D genes of FMDV was packaged into VLP with EP141-160 presented on the surface. ELISA and Western blotting proved that the epitope-RNA VLP eliciting an immune response to FMDV in mice. Furthermore, the potency of the vaccine was tested in mice and guinea pigs. The results indicated that the epitope-RNA VLP vaccine protected 40% of suckling mice and 85% (17/20) of guinea pigs from FMDV. Based on the experimental data, the epitope-RNA VLP vaccine should have value in exploring and developing vaccines against FMDV in the future.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Dong YM,Cai JC,Chen HT,Chen Ldoi
10.1016/j.antiviral.2016.08.020subject
Has Abstractpub_date
2016-10-01 00:00:00pages
108-116eissn
0166-3542issn
1872-9096pii
S0166-3542(16)30455-7journal_volume
134pub_type
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更新日期：2013-08-01 00:00:00
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pub_type： 临床试验,杂志文章
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更新日期：2007-12-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2011-12-01 00:00:00
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更新日期：2017-02-01 00:00:00
