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A bacterial artificial chromosome (BAC)-vectored noninfectious replicon of SARS-CoV-2.

Abstract：

:Vaccines and antiviral agents are in urgent need to stop the COVID-19 pandemic. To facilitate antiviral screening against SARS-CoV-2 without requirement for high biosafety level facility, we developed a bacterial artificial chromosome (BAC)-vectored replicon of SARS-CoV-2, nCoV-SH01 strain, in which secreted Gaussia luciferase (sGluc) was encoded in viral subgenomic mRNA as a reporter gene. The replicon was devoid of structural genes spike (S), membrane (M), and envelope (E). Upon transfection, the replicon RNA replicated in various cell lines, and was sensitive to interferon alpha (IFN-α), remdesivir, but was resistant to hepatitis C virus inhibitors daclatasvir and sofosbuvir. Replication of the replicon was also sensitive overexpression to zinc-finger antiviral protein (ZAP). We also constructed a four-plasmid in-vitro ligation system that is compatible with the BAC system, which makes it easy to introduce desired mutations into the assembly plasmids for in-vitro ligation. This replicon system would be helpful for performing antiviral screening and dissecting virus-host interactions.

journal_name

Antiviral Res

journal_title

Antiviral research

authors

Zhang Y,Song W,Chen S,Yuan Z,Yi Z

doi

10.1016/j.antiviral.2020.104974

subject

Has Abstract

pub_date

2021-01-01 00:00:00

pages

104974

eissn

0166-3542

issn

1872-9096

pii

S0166-3542(20)30388-0

journal_volume

185

pub_type

杂志文章

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