Abstract：
:Infectious bursal disease (IBD) leads to considerable economic losses for the poultry industry by inducing severe immunosuppression and high mortality in chickens. The objective of this study was to determine if RNA interference (RNAi) could be utilized to inhibit IBDV replication in vitro. We selected 3 short interfering RNA (siRNA) sequences (siVP1(618), siVP1(1,115), and siVP1(2,571)) based on conserved regions in the vp1 gene of the infectious bursal disease virus (IBDV). When the Vero cells were transfected with siRNA, synthesized via in vitro transcription, and then infected with IBDV, siVP1(2,571) was discovered to be the most effective site for inhibiting IBDV replication. For long-term expression of siRNA and due to its suitability for large-scale preparation, the mouse U6 promoter was amplified using primers designed according to the siVP1(2,571) sequence. The resulting products were then subcloned into pEGFP-C1 to construct the shRNA expression vector pEC2571-shRNA. The shRNA-transfected Vero cells were then infected with IBDV. As compared to the control, the inhibitory rate in the pEC2,571-shRNA-transfected group was 87.4%. Indirect immunofluorescence and real-time polymerase chain reaction (PCR) confirmed that VP1 expression decreased at both the protein and RNA levels as compared to that in the controls. The results presented here indicate that DNA vector-based RNAi could effectively inhibit IBDV replication in vitro.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Gao Y,Liu W,Gao H,Qi X,Lin H,Wang X,Shen Rdoi
10.1016/j.antiviral.2007.11.007subject
Has Abstractpub_date
2008-08-01 00:00:00pages
87-94issue
2eissn
0166-3542issn
1872-9096pii
S0166-3542(07)00472-Xjournal_volume
79pub_type
杂志文章abstract：:Transcriptional activation of HIV-1 gene expression is partially controlled by the interaction between viral and cellular transcription factors acting at HIV-1 long terminal repeat (LTR) sequences. HIV-1 subtyping at LTR region and nucleotide LTR variability from clinical samples in 48 subjects carrying different HIV-...
journal_title：Antiviral research
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更新日期：2010-11-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：1999-12-15 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(87)90085-4
更新日期：1987-08-01 00:00:00
abstract：UNLABELLED:This paper presents the public health rationale for multipurpose prevention technologies (MPTs) for sexual and reproductive health (SRH) based on regional trends in demographic and SRH indicators. It then distils important lessons gleaned from the introduction of contraceptive and reproductive health product...
journal_title：Antiviral research
pub_type： 杂志文章,评审
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更新日期：2013-12-01 00:00:00
abstract：:The 27th International Conference on Antiviral Research (ICAR) was held in Raleigh, North Carolina, USA from May 12 to 16, 2014. This article summarizes the principal invited lectures. John Drach (Elion Award) described the early days of antiviral drugs and their novel modes of action. Piet Herdewijn (Holý Award) used...
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更新日期：2008-12-01 00:00:00
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journal_title：Antiviral research
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更新日期：1985-12-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(90)90040-e
更新日期：1990-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2008.06.011
更新日期：2008-12-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
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更新日期：2020-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0166-3542(93)90082-t
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abstract：:Epidermal growth factor receptor (EGFr) has been shown to be induced and activated in cells infected with HPV, suggesting that it may play a physiological role in viral replication or in the formation or maintenance of warts. To investigate this possibility, human foreskin tissue was infected with HPV11 and transplant...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.12.004
更新日期：2007-04-01 00:00:00
abstract：:Pritelivir, a helicase-primase inhibitor, has excellent in vitro and in vivo activity against human herpes simplex virus (HSV). Mice lethally infected with HSV type 1 or 2, including acyclovir-resistant strains, were treated 72 h after infection for 7 days with pritelivir or acyclovir. Both drugs were administered ora...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2017.11.002
更新日期：2018-01-01 00:00:00
abstract：:Cytomegalovirus (CMV) is the most common cause of congenital infection in the developed world and can lead to a life-threatening disease. We therefore developed an animal model to evaluate candidate anti-CMV drugs and to further define the pathogenesis of CMV infections. Newborn guinea pigs were infected by intraperit...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(03)00151-7
更新日期：2003-09-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2013.06.011
更新日期：2013-09-01 00:00:00
abstract：:Oligodeoxynucleotides (ODNs) composed entirely of deoxyguanosine and thymidine, but not specifically designed to act as antisense agents, were able to significantly inhibit herpes simplex virus growth in acute infection assay systems. The guanosine/thymidine (GT) ODNs which demonstrated this antiviral activity contain...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(94)00064-f
更新日期：1995-01-01 00:00:00
abstract：:Almost 30 years ago it was proposed that the selection for antiviral drug resistance should be used as an indicator of antiviral drug activity. In addition to discriminating between cellular toxicity and specific activity directed against a viral target, drug resistant mutants have been used to confirm the mechanism o...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2006.03.004
更新日期：2006-09-01 00:00:00
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journal_title：Antiviral research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.antiviral.2005.04.001
更新日期：2005-07-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(96)00946-1
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(88)90003-4
更新日期：1988-04-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(84)90033-0
更新日期：1984-10-01 00:00:00
abstract：:Carbovir (CBV) is a guanine nucleoside analog with potent in vitro anti-HIV activity. A prodrug of CBV is currently being evaluated in clinical trials as a potential agent for the treatment of AIDS. The ability of CBV to inhibit mitochondrial DNA synthesis in intact CEM cells was evaluated in the present study, becaus...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(97)01033-4
更新日期：1997-05-01 00:00:00
abstract：:Influenza A virus has been responsible for widespread human epidemics because it is readily transmitted from humans to humans by aerosol. Recent events have highlighted the potential of influenza A virus as a bioterrorist weapon: the high virulence of the influenza A virus that infected people in Hong Kong in 1997; an...
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pub_type： 杂志文章,评审
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更新日期：2003-01-01 00:00:00
abstract：:A major challenge in the development of vaccines against retroviruses is the induction of neutralizing antibodies since they prevent infection of the cells where the virus may persist. The transmembrane envelope (TM) protein contains highly conserved domains and seems to be a suitable target. To study whether vaccinat...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2010.11.011
更新日期：2011-01-01 00:00:00
abstract：BACKGROUND/AIMS:Traditional Chinese herbal therapies are widely used for the treatment of chronic hepatitis C (CHC) in Asia. The aim of this study was to perform a meta-analysis of randomised controlled trials (RCTs) comparing interferon therapies with Chinese herbal therapies and/or interferon plus Chinese herb therap...
journal_title：Antiviral research
pub_type： 杂志文章,meta分析
doi：10.1016/j.antiviral.2010.12.003
更新日期：2011-02-01 00:00:00
abstract：:An estimated 15-20 million individuals are co-infected by hepatitis B and hepatitis D virus worldwide and are at high risk of developing end-stage liver disease, including hepatocellular carcinoma. While HBV viremia can now be controlled in the vast majority of individuals by nucleoside analogs, leading to a delay of ...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2015.08.009
更新日期：2015-10-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2014.01.020
更新日期：2014-04-01 00:00:00
abstract：:In an ongoing effort to identify an orally bioavailable compound for the treatment of rhino- and enteroviral infections, a series of vinylacetylene benzimidazoles was recently examined. Previous studies demonstrated the potential for these compounds to possess both good in vitro antiviral activity as well as acceptabl...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(99)00013-3
更新日期：1999-05-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/s0166-3542(01)00195-4
更新日期：2001-12-01 00:00:00
abstract：:Antiviral therapies for chronic hepatitis B virus (HBV) infection that are currently applicable for clinical use are limited to nucleos(t)ide analogs targeting HBV polymerase activity and pegylated interferon alpha (PEG-IFN). Towards establishing an effective therapy for HBV related diseases, it is important to develo...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2017.06.005
更新日期：2017-08-01 00:00:00
