Abstract：
:Human adenoviruses are a common threat to immunocompromised patients, e.g., HIV-positive individuals or solid-organ and, in particular, allogeneic stem cell transplant recipients. Antiviral drugs have a limited effect on adenoviruses, and existing treatment modalities often fail to prevent fatal outcome. Silencing of viral genes by short interfering RNAs (siRNAs) holds a great promise in the treatment of viral infections. The aim of the present study was to identify adenoviral candidate targets for RNA interference-mediated inhibition of adenoviral replication. We investigated the impact of silencing of a set of early, middle, and late viral genes on the replication of adenovirus 5 in vitro. Adenovirus replication was inhibited by siRNAs directed against the adenoviral E1A, DNA polymerase, preterminal protein (pTP), IVa2, hexon, and protease genes. Silencing of early and middle genes was more effective in inhibiting adenovirus multiplication than was silencing of late genes. A siRNA directed against the viral DNA polymerase mRNA decreased viral genome copy numbers and infectious virus progeny by several orders of magnitude. Since silencing of any of the early genes directly or indirectly affected viral DNA synthesis, our data suggest that reducing viral genome copy numbers is a more promising strategy for the treatment of adenoviral infections than is reducing the numbers of proteins necessary for capsid generation. Thus, adenoviral DNA replication was identified as a key target for RNAi-mediated inhibition of adenovirus multiplication. In addition, the E1A transcripts emerged as a second important target, because its knockdown markedly improved the viability of cells at late stages of infection.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Kneidinger D,Ibrišimović M,Lion T,Klein Rdoi
10.1016/j.antiviral.2012.03.011subject
Has Abstractpub_date
2012-06-01 00:00:00pages
195-207issue
3eissn
0166-3542issn
1872-9096pii
S0166-3542(12)00079-4journal_volume
94pub_type
杂志文章abstract：:Twenty-five years after the discovery of the therapeutic activity of azidothymidine (AZT), the first antiretroviral drug used in the clinic, infection with the human immunodeficiency virus (HIV) has become, at least in the industrialized world, a manageable chronic disease with a significant improvement in life expect...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2009.10.007
更新日期：2010-01-01 00:00:00
abstract：:The prevention and control of disease caused by seasonal and potential pandemic influenza viruses is currently managed by the use influenza vaccines and antivirals. The adamantanes (amantadine and rimantadine) were the first antivirals licensed for use against influenza A viruses and have been used extensively in some...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.08.002
更新日期：2007-02-01 00:00:00
abstract：:The dengue fever virus (DENV) and the yellow fever virus (YFV) are members of the genus flavivirus in the family Flaviviridae. An estimated 50-100 million cases of DENV infections occur each year and approximately half a million patients require hospitalization. There is no vaccine or effective antiviral treatment ava...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2013.01.011
更新日期：2013-05-01 00:00:00
abstract：:There is a pressing need for new vaccines against alphaviruses, which can cause fatal encephalitis (Venezuelan equine encephalitis virus (VEEV) and others) and severe arthralgia (e.g. Chikungunya virus, CHIKV). These positive-strand RNA viruses are diverse and evolve rapidly, meaning that the sequence of any vaccine s...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104905
更新日期：2020-10-01 00:00:00
abstract：:Thirty-six adult volunteers were inoculated intranasally with 7.2 log10 egg infectious doses 50% of an attenuated A/Khabarovsk/77/H1N1 virus. Twenty-four hours later volunteers were begun on both aerosol treatments (rimantadine HCl 25 mg in saline or saline, 10 min exposure twice daily) and oral medications (rimantadi...
journal_title：Antiviral research
pub_type： 临床试验,杂志文章
doi：10.1016/0166-3542(82)90016-x
更新日期：1982-08-01 00:00:00
abstract：:We investigated the development of anti-pre-S(2) antibodies by enzyme immunoassay and by Western blot analysis in a vaccination study in haemodialysis patients; both the Pasteur plasma vaccine retaining the pre-S(2) epitopes and the Merck, Sharp and Dohme (MSD) plasma vaccine containing only HBsAg were used. By enzyme...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(89)90071-5
更新日期：1989-09-01 00:00:00
abstract：:Current therapies for chronic hepatitis B virus infection (CHB) - nucleos(t)ide analogue reverse transcriptase inhibitors and interferons - result in low rates of functional cure defined as sustained off-therapy seroclearance of hepatitis B surface antigen (HBsAg). One likely reason is the inability of these therapies...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2015.06.019
更新日期：2015-09-01 00:00:00
abstract：:This study describes the extent of cross-resistance and interactions for selected inhibitors of human cytomegalovirus (HCMV) DNA synthesis and DNA processing. HCMV isolates resistant to the benzimidazole D-ribonucleoside viral DNA processing inhibitors TCRB and BDCRB were sensitive to BAY 38-4766, a non-nucleoside inh...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(02)00094-3
更新日期：2002-10-01 00:00:00
abstract：:Infection with Junín virus (JUNV) is currently being effectively managed in the endemic region using a combination of targeted vaccination and plasma therapy. However, the long-term sustainability of plasma therapy is unclear and similar resources are not available for other New World arenaviruses. As a result, there ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2018.07.009
更新日期：2018-09-01 00:00:00
abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent of Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease. In immunocompromised patients, KSHV infection is capable of causing severe and fatal diseases. Current antiviral treatments for KSHV infections consist mostly of n...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104825
更新日期：2020-08-01 00:00:00
abstract：BACKGROUND/AIMS:Traditional Chinese herbal therapies are widely used for the treatment of chronic hepatitis C (CHC) in Asia. The aim of this study was to perform a meta-analysis of randomised controlled trials (RCTs) comparing interferon therapies with Chinese herbal therapies and/or interferon plus Chinese herb therap...
journal_title：Antiviral research
pub_type： 杂志文章,meta分析
doi：10.1016/j.antiviral.2010.12.003
更新日期：2011-02-01 00:00:00
abstract：:2-Furylmercury chloride (2-FMC), an organic mercury derivative, has been found to inhibit the replication of all tested human rhinovirus (HRV) serotypes belonging to the antiviral group B and a limited number of HRV serotypes belonging to the antiviral group A. The mechanism of action of 2-FMC was tested against HRV-2...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2003.10.004
更新日期：2004-03-01 00:00:00
abstract：:Several mammarenaviruses, chiefly Lassa virus (LASV) in Western Africa and Junín virus (JUNV) in the Argentine Pampas, cause severe disease in humans and pose important public health problems in their endemic regions. Moreover, mounting evidence indicates that the worldwide-distributed mammarenavirus lymphocytic chori...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2019.104558
更新日期：2019-09-01 00:00:00
abstract：:Entry of human immunodeficiency virus type 1 (HIV-1) into target cells is mediated by its envelope protein gp41 through membrane fusion. Interaction of two extra-virion heptad repeats (HRs) in the gp41 plays a pivotal role in the fusion, and its inhibitor, enfuvirtide (T-20), blocks HIV-1 entry. To identify agents tha...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2008.05.006
更新日期：2008-10-01 00:00:00
abstract：:Three water-soluble derivatives of amphotericin B were tested for inhibition of HIV infection in vitro. The compounds amphotericin B methyl ester (AME) and N-(N'-(2-(4'-methylmorpholinio)ethyl)N"-cyclohexyl guanyl) amphotericin B methyl ester (MCG) inhibited HIV infection by 50% at 1 microgram/ml; N-(N'-(3-dimethylami...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(90)90031-2
更新日期：1990-09-01 00:00:00
abstract：:The internal ribosome entry site (IRES) of hepatitis C virus (HCV) which governs the initiation of protein synthesis from viral RNA represents an ideal target for antisense approaches. Using an original bicistronic plasmid, we first established that sequence and translational activity of HCV IRESs cloned from six pati...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2008.06.011
更新日期：2008-12-01 00:00:00
abstract：:Lactoferrin (LF) is an iron binding glycoprotein that is present in several mucosal secretions. Many biological functions have been ascribed to LF. One of the functions of LF is the transport of metals, but LF is also an important component of the non-specific immune system, since LF has antimicrobial properties again...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/s0166-3542(01)00195-4
更新日期：2001-12-01 00:00:00
abstract：:The first 230 residues of the 298-amino acid glycoprotein G of respiratory syncytial virus (RSV) are sufficient to confer complete resistance to challenge with live RSV, whereas the first 180 residues completely failed (Olmsted et al. (1989) J. Virol. 63, 411-420). The characterization of a protective epitope correspo...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(95)00053-4
更新日期：1995-12-01 00:00:00
abstract：:MicroRNA-122 (miR-122) is involved in the pathogenesis of several liver diseases, including chronic hepatitis B infection and hepatocellular carcinoma. This study aimed to explore the potential role of miR-122 in the interferon (IFN)-mediated suppression of hepatitis B virus (HBV) in hepatocytes. We found that elevate...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2015.03.001
更新日期：2015-06-01 00:00:00
abstract：:HIV-host infection systems in vitro are important in the pre-clinical assessment of anti-retroviral drug activity. The present report describes the development of a new HIV-host model comprised of an epithelial cell line of HeLa lineage (HeLa-1), transfected with expression vectors bearing tat and rev (TART) genes of ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(99)00014-5
更新日期：1999-07-01 00:00:00
abstract：:Chikungunya virus (CHIKV) is an arthropod-borne alphavirus. Alphaviruses are positive strand RNA viruses that require a 5' cap structure to direct translation of the viral polyprotein and prevent degradation of the viral RNA genome by host cell nucleases. Formation of the 5' RNA cap is orchestrated by the viral protei...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2018.03.013
更新日期：2018-06-01 00:00:00
abstract：:Almost all cases of human rabies result from dog bites, making the elimination of canine rabies a global priority. During recent decades, many countries in the Western Hemisphere have carried out large-scale dog vaccination campaigns, controlled their free-ranging dog populations and enforced legislation for responsib...
journal_title：Antiviral research
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.antiviral.2017.03.023
更新日期：2017-07-01 00:00:00
abstract：:Serious outbreaks of severe acute respiratory syndrome (SARS), caused by the newly discovered coronavirus SARS-CoV, occurred between late 2002 and early 2003 and there is an urgent need for effective antiviral agents. RNA interference in animals and post-transcriptional gene silencing plants is mediated by small doubl...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2004.09.005
更新日期：2005-01-01 00:00:00
abstract：:Respiratory syncytial virus (RSV) is the major cause for respiratory tract disease in infants and young children. Currently, no licensed vaccine or a selective antiviral drug against RSV infections are available. Here, we describe a structure-based drug design approach that led to the synthesis of a novel series of zi...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2015.08.003
更新日期：2015-10-01 00:00:00
abstract：:Oligodeoxynucleotides (ODN) containing unmethylated CpG motifs are potent stimulators of the innate immune system, and are capable of aborting several infections in a non-specific manner. We here report studies of the capacity of such ODN to protect mice against infection with foot and mouth disease virus (FMDV). Susc...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.03.010
更新日期：2006-10-01 00:00:00
abstract：:Respiratory syncytial virus (RSV) infection causes significant disease in the lower respiratory tract of young children, and there is currently no licensed vaccine to prevent RSV infection. The F glycoprotein is considered a major antigenic target for RSV vaccine development. Recent evidence indicates that the pre-fus...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2019.02.017
更新日期：2019-05-01 00:00:00
abstract：:Hepatitis C virus (HCV) infection is a major cause of chronic liver diseases such as steatosis, cirrhosis, and hepatocellular carcinoma. HCV particles have been found to associate with apolipoproteins, and apolipoproteins not only participate in the HCV life cycle, but also help HCV escape recognition by the host immu...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104756
更新日期：2020-05-01 00:00:00
abstract：:The bicyclams represent a new class of highly potent and selective HIV inhibitors. Time-of-addition experiments have previously shown that these compounds interfere with an early event in the viral replicative cycle. Additional experiments have now been carried out in order to investigate in more detail the mechanism ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(95)00837-3
更新日期：1996-03-01 00:00:00
abstract：:Due to diverse pathogenic potentials, there is a growing need for anti-HCMV agents. In this study, we show that treatment with DAPT, a γ-secretase inhibitor (GSI), impairs HCMV replication as assessed by a progeny assay based on immunostaining. This effect is not limited to DAPT because other GSIs with different struc...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104867
更新日期：2020-11-01 00:00:00
abstract：:The global analysis of neuraminidase inhibitor (NAI) susceptibility of influenza viruses has been conducted since the 2012-13 period. In 2018 a novel cap-dependent endonuclease inhibitor, baloxavir, that targets polymerase acidic subunit (PA) was approved for the treatment of influenza virus infection in Japan and the...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104718
更新日期：2020-03-01 00:00:00
