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Inhibition of SARS-CoV replication by siRNA.

Abstract：

:Serious outbreaks of severe acute respiratory syndrome (SARS), caused by the newly discovered coronavirus SARS-CoV, occurred between late 2002 and early 2003 and there is an urgent need for effective antiviral agents. RNA interference in animals and post-transcriptional gene silencing plants is mediated by small double-stranded RNA molecules named small interfering RNA (siRNA). Recently, siRNA-induced RNA interference(RNAi) may provide a new approach to therapy for pathogenic viruses, e.g. HIV and HCV. In this study, the silencing potential of seven synthetic siRNAs against SARS-CoV leader, TRS, 3'-UTR and Spike coding sequence have been applied to explore the possibility for prevention of SARS-CoV infection. We demonstrate that siRNAs directed against Spike sequences and the 3'-UTR can inhibit the replication of SARS-CoV in Vero-E6 cells, and holds out promise for the development of an effective antiviral agent against SARS-CoV.

journal_name

Antiviral Res

journal_title

Antiviral research

authors

Wu CJ,Huang HW,Liu CY,Hong CF,Chan YL

doi

10.1016/j.antiviral.2004.09.005

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

45-8

issue

1

eissn

0166-3542

issn

1872-9096

pii

S0166-3542(04)00222-0

journal_volume

65

pub_type

杂志文章

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