Abstract:
:Trimming of the N-glycans attached to the envelope proteins of hepatitis B virus (HBV) is required in different steps of the viral life cycle. Inhibition of the host enzymes alpha-glucosidases, involved in the endoplasmic reticulum (ER)-associated processing of the N-linked glycans, results in misfolding of the HBV envelope proteins, prevention of HBV secretion and accumulation of viral DNA within infected cells. However, the impact of these effects on HBV morphogenesis and infectivity of the viral particles that are still released from cells with inhibited alpha-glucosidase has not been addressed so far. Using N-butyldeoxynojirimycin (NB-DNJ), a competitive inhibitor of the ER alpha-glucosidases, we analyzed the role of these enzymes on HBV assembly and infectivity of the virions released from HepG2.2.2.15 cells. HBV secreted from drug-treated cells contained an envelope with altered composition of the disulfide-linked oligomers and no detectable middle (M) protein. These molecular changes had a significant effect on HBV infectivity, reducing it to 20% compared to controls, for the highest concentrations of NB-DNJ used. Our data show for the first time that an active alpha-glucosidase activity is crucial for production of infectious HBV and provide new insights into the controversial role of the M protein in this process.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Lazar C,Durantel D,Macovei A,Zitzmann N,Zoulim F,Dwek RA,Branza-Nichita Ndoi
10.1016/j.antiviral.2007.04.004subject
Has Abstractpub_date
2007-10-01 00:00:00pages
30-7issue
1eissn
0166-3542issn
1872-9096pii
S0166-3542(07)00327-0journal_volume
76pub_type
杂志文章abstract::Influenza viruses (IV) continue to pose an imminent threat to human welfare. Yearly re-occurring seasonal epidemic outbreaks and pandemics with high mortality can occur. Besides vaccination against a limited number of viral strains only a few antiviral drugs are available, which are losing their effectiveness as more ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2017.03.024
更新日期:2017-06-01 00:00:00
abstract::Synthetic flavans, isoflavans and isoflavenes substituted with chloro, cyano and amidino groups were tested for their in vitro activity against poliovirus type 2, Coxsackie virus B4, echovirus type 6 and enterovirus 71. Plaque-reduction assays showed that substituted 3-(2H)-isoflavenes, carrying a double bond in the o...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(95)00088-4
更新日期:1995-05-01 00:00:00
abstract::Interleukin (IL)28-B polymorphism has been related to interferon response in the treatment of hepatitis C, but its role in chronic hepatitis B (CHB) therapy is still poorly understood. We aimed to investigate the effect of IL28-B polymorphisms in the treatment with pegylated-interferon (PEG-IFN) of patients with CHB. ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2013.11.014
更新日期:2014-02-01 00:00:00
abstract::Antigen presenting liposomes (APLs) containing both liposome encapsulated (44%) and free (56%) recombinant glycoprotein D of herpes simplex virus type-2 (rgD-2) were characterized with respect to the interaction of the antigen with the lipid bilayer and the biological activities provided by each form of rgD-2. We foun...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(90)90037-8
更新日期:1990-04-01 00:00:00
abstract::Resveratrol (RV), a polyphenolic natural product present in many plants and fruits, exhibits anti-inflammatory, cardio-protective and anti-proliferative properties. Moreover, RV affects a wide variety of viruses including members of the Herpesviridae family, retroviruses, influenza A virus and polyomavirus by altering...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2012.09.003
更新日期:2012-11-01 00:00:00
abstract::Previous studies of IFN-stimulated transcription factors in murine cells have identified a variety of trans-acting factors that bind to the IFN-stimulated response element (ISRE) whose role in gene expression remain unclear. The present investigation was undertaken to delineate the signal transduction pathway as well ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(94)90099-x
更新日期:1994-10-01 00:00:00
abstract::SeqHepB is a combination of a HBV genome sequence analysis program and a relational database that houses data collected from multiple data sources. Registered users can access the sequence analysis component of SeqHepB online for rapid and detailed interrogation of HBV genomic sequences. Its main function is to determ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2006.11.014
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:Lactoferrin (LF) is a glycoprotein present in human milk with known antimicrobial effects. In vitro, LF has demonstrated antiviral activity against respiratory syncytial virus (RSV). We sought to assess the effect of bovine (b)LF in RSV replication, lung inflammation and function, cytokine profiles and clini...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2013.05.013
更新日期:2013-08-01 00:00:00
abstract::This review describes the procedures for the use of fluorochrome labeled monoclonal antibodies and flow cytometry for the detection and quantification of virus infected cells. The application of this technology for (1) identifying virus infected cells in clinical specimens obtained from human cytomegalovirus (HCMV) an...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/s0166-3542(99)00039-x
更新日期:1999-08-01 00:00:00
abstract:BACKGROUND:By modulating the antiviral immune response via vitamin D receptor, the active form of vitamin D (1,25-dihydroxyvitamin D, calcitriol) could play a central role in protection against respiratory virus infections. This in vitro study tested the hypothesis that respiratory viruses modulate vitamin D receptor e...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.11.004
更新日期:2017-01-01 00:00:00
abstract::Three water-soluble derivatives of amphotericin B were tested for inhibition of HIV infection in vitro. The compounds amphotericin B methyl ester (AME) and N-(N'-(2-(4'-methylmorpholinio)ethyl)N"-cyclohexyl guanyl) amphotericin B methyl ester (MCG) inhibited HIV infection by 50% at 1 microgram/ml; N-(N'-(3-dimethylami...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(90)90031-2
更新日期:1990-09-01 00:00:00
abstract::Dengue virus (DENV) infection is one of the most important infectious diseases in tropical and subtropical regions around the world. Previously, we performed an initial phenotypic screening of 7000 compounds using DENV type 2 (DENV2)-infected BHK-21 cells to identify small molecules which could inhibit virus replicati...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2020.104969
更新日期:2020-12-01 00:00:00
abstract::Anti-herpes simplex virus (HSV) activities of oxyresveratrol in vitro and topical administration in cutaneous HSV-1 infection in mice were examined. The inhibitory concentrations for 50% plaque formation (IC(50)) of oxyresveratrol against HSV-1 clinical isolates and HSV-2 clinical isolates were 20.9-29.5 and 22.2-27.5...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2011.05.013
更新日期:2011-08-01 00:00:00
abstract::Carbovir (CBV) is a guanine nucleoside analog with potent in vitro anti-HIV activity. A prodrug of CBV is currently being evaluated in clinical trials as a potential agent for the treatment of AIDS. The ability of CBV to inhibit mitochondrial DNA synthesis in intact CEM cells was evaluated in the present study, becaus...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(97)01033-4
更新日期:1997-05-01 00:00:00
abstract::Because encapsulation of antiviral drugs in liposomes resulted generally in improved activity against retroviral replication in vivo, the antiviral effects of free-SPC3 and liposome-associated SPC3 were compared in cultured human lymphocytes infected with HIV-1. SPC3 was entrapped in various liposomal formulations, ei...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(02)00002-5
更新日期:2002-06-01 00:00:00
abstract::Vaccination against smallpox may result in a variety of complications, ranging in severity from benign to lethal. Universal vaccination was halted in the US in 1972, so almost half the present population has never been vaccinated. Because side effects occur most often in first-time vaccinees, current plans for rapid l...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/s0166-3542(03)00008-1
更新日期:2003-04-01 00:00:00
abstract::Natural killer (NK) cells are a crucial component of the host innate immune system. We investigated the noncytolytic anti-human immunodeficiency virus (HIV) activity of NK cells in chronically HIV-infected immune cells. Supernatants collected from NK cell cultures (both primary NK cells and NK cell lines, YTS and NK 9...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2006.08.006
更新日期:2007-02-01 00:00:00
abstract::DNA immunizations with the major structural protein VP1 of Coxsackie virus B3 (CVB3) have been previously found to protect BALB/c mice from lethal challenge. Here we report that the other CVB3 capsid proteins, VP2, VP3, and VP4, were less effective at preventing CVB3-caused disease. The application of pCMV/VP1 as a va...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(00)00132-7
更新日期:2001-01-01 00:00:00
abstract::On September 22, 2008, a physician on Prince of Wales Island, Alaska, notified the Alaska Department of Health and Social Services (ADHSS) of an unusually high number of adult patients with recently diagnosed pneumonia (n = 10), including three persons who required hospitalization and one who died. ADHSS and CDC condu...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.08.004
更新日期:2018-10-01 00:00:00
abstract::The 27th International Conference on Antiviral Research (ICAR) was held in Raleigh, North Carolina, USA from May 12 to 16, 2014. This article summarizes the principal invited lectures. John Drach (Elion Award) described the early days of antiviral drugs and their novel modes of action. Piet Herdewijn (Holý Award) used...
journal_title:Antiviral research
pub_type:
doi:10.1016/j.antiviral.2014.08.009
更新日期:2014-11-01 00:00:00
abstract::AIDS therapies employing HIV protease inhibitors (PIs) are associated with changes in fat metabolism. However, the cellular mechanisms affected by PIs are not clear. Thus, the affects of PIs on adipocyte differentiation were examined in vitro using C3H10T1/2 stem cells. In these cells the PIs, nelfinavir, saquinavir, ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(00)00102-9
更新日期:2000-08-01 00:00:00
abstract::Prunellin, an anti-HIV active compound, was isolated from aqueous extracts of the Chinese medicinal herb, Prunella vulgaris, and purified to chromatographic homogeneity. Infrared and NMR spectroscopy identified prunellin as a polysaccharide. Elemental analyses, precipitation with calcium(II), barium(II), or 9-aminoacr...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(89)90036-3
更新日期:1989-06-01 00:00:00
abstract::Nucleoside analogues have been the most successful antiviral compounds. Likewise, they are the most intriguing antiviral compounds, because of their structural relationship to natural nucleosides. This is also the reason why the design process of a potential selective antiviral nucleoside is so difficult. Too many nat...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2006.04.004
更新日期:2006-09-01 00:00:00
abstract::Entry of human immunodeficiency virus type 1 (HIV-1) into target cells is mediated by its envelope protein gp41 through membrane fusion. Interaction of two extra-virion heptad repeats (HRs) in the gp41 plays a pivotal role in the fusion, and its inhibitor, enfuvirtide (T-20), blocks HIV-1 entry. To identify agents tha...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2008.05.006
更新日期:2008-10-01 00:00:00
abstract::Infections by dengue virus (DENV) are increasing worldwide, with an urgent need for effective anti-DENV agents. We recently identified N-(4-hydroxyphenyl) retinamide (4-HPR), an anti-DENV agent effective against all 4 serotypes of DENV in cell culture, and in a lethal mouse model for DENV infection (Fraser et al., 201...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.03.006
更新日期:2016-06-01 00:00:00
abstract::Adult woodchucks (Marmota monax) chronically infected with woodchuck hepatitis virus (WHV) were treated orally with lamivudine (15 mg/kg per day) for 57 weeks. After 20 weeks of treatment a 2-3 log reduction in serum WHV DNA was detected. Serum titers of WHV then increased gradually, in the presence of lamivudine trea...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2004.03.005
更新日期:2004-08-01 00:00:00
abstract::An immunogenic component was isolated from both the Illinois (propagated in young swine) and the Miller (cell culture-adapted) strains of porcine transmissible gastroenteritis (TGE) virus. The viral subunit was released from the virion by sonication and was separated from intact virus and other viral components by iso...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(83)90027-x
更新日期:1983-09-01 00:00:00
abstract::The antiviral effect of foscarnet (PFA) on the replication of rotavirus, a member of the Reoviridae, was studied. The pyrophosphate analogue is an effective inhibitor of several viral polymerases acting on the enzyme pyrophosphate binding site. Replication of rotavirus in MA104 cells using different u.o.i. was inhibit...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(94)00085-m
更新日期:1995-05-01 00:00:00
abstract::Acute lower respiratory tract infections (ALRI) caused by respiratory syncytial virus (RSV) are associated with a severe disease burden among infants and elderly patients. Treatment options are limited. While numerous drug candidates with different viral targets are under development, the utility of RSV entry inhibito...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2020.104774
更新日期:2020-05-01 00:00:00
abstract::Infection with Junín virus (JUNV) is currently being effectively managed in the endemic region using a combination of targeted vaccination and plasma therapy. However, the long-term sustainability of plasma therapy is unclear and similar resources are not available for other New World arenaviruses. As a result, there ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.07.009
更新日期:2018-09-01 00:00:00