Abstract：
:This review describes the procedures for the use of fluorochrome labeled monoclonal antibodies and flow cytometry for the detection and quantification of virus infected cells. The application of this technology for (1) identifying virus infected cells in clinical specimens obtained from human cytomegalovirus (HCMV) and human immunodeficiency virus (HIV) infected individuals; (2) screening antiviral compounds active against HCMV, HDSV and HIV; and (3) performing drug susceptibility testing for HCMV, HSV and HIV clinical isolates are reviewed. The flow cytometry drug susceptibility assay is rapid, quantitative, and easily performed. It should be considered by anyone interested in performing drug susceptibility testing for any virus for which there are reliable monoclonal antibodies.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
McSharry JJdoi
10.1016/s0166-3542(99)00039-xsubject
Has Abstractpub_date
1999-08-01 00:00:00pages
1-21issue
1eissn
0166-3542issn
1872-9096pii
S016635429900039Xjournal_volume
43pub_type
杂志文章,评审abstract：:Ribonucleases H (RNases H) are endonucleolytic enzymes, evolutionarily related to retroviral integrases, DNA transposases, resolvases and numerous nucleases. RNases H cleave RNA in RNA/DNA hybrids and their activity plays an important role in the replication of prokaryotic and eukaryotic genomes, as well as in the rep...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2019.104613
更新日期：2019-11-01 00:00:00
abstract：BACKGROUND:Prophylaxis is widely adopted the best choice against Hemorrhagic fever with renal syndrome (HFRS) caused by Hantavirus. However, loss of memory immune response maintenance remains as major shortcoming in current HFRS vaccine. A recombinant DNA vaccine, pVAX-LAMP/Gn was previously proved efficient, requiring...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.12.001
更新日期：2017-02-01 00:00:00
abstract：:We investigated the development of anti-pre-S(2) antibodies by enzyme immunoassay and by Western blot analysis in a vaccination study in haemodialysis patients; both the Pasteur plasma vaccine retaining the pre-S(2) epitopes and the Merck, Sharp and Dohme (MSD) plasma vaccine containing only HBsAg were used. By enzyme...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(89)90071-5
更新日期：1989-09-01 00:00:00
abstract：:Rabies is a lethal viral infection that can affect almost all mammals, including humans. To better understand the replication of Rabies lyssavirus, we investigated if the viral load in brains naturally infected with rabies influences viral internalization and viral growth kinetics in neuroblastoma cells, and if the vi...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2017.11.003
更新日期：2018-01-01 00:00:00
abstract：:The dengue fever virus (DENV) and the yellow fever virus (YFV) are members of the genus flavivirus in the family Flaviviridae. An estimated 50-100 million cases of DENV infections occur each year and approximately half a million patients require hospitalization. There is no vaccine or effective antiviral treatment ava...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2013.01.011
更新日期：2013-05-01 00:00:00
abstract：BACKGROUND/AIMS:Traditional Chinese herbal therapies are widely used for the treatment of chronic hepatitis C (CHC) in Asia. The aim of this study was to perform a meta-analysis of randomised controlled trials (RCTs) comparing interferon therapies with Chinese herbal therapies and/or interferon plus Chinese herb therap...
journal_title：Antiviral research
pub_type： 杂志文章,meta分析
doi：10.1016/j.antiviral.2010.12.003
更新日期：2011-02-01 00:00:00
abstract：:Zika virus (ZIKV) is a mosquito-borne flavivirus associated with severe neonatal birth defects, but the causative mechanism is incompletely understood. ZIKV shares sequence homology and early clinical manifestations with yellow fever virus (YFV) and dengue virus (DENV) and are all transmitted in urban cycles by the sa...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2018.01.003
更新日期：2018-03-01 00:00:00
abstract：:As part of our studies on the molecular basis for the antiherpes activity of 5'-AdThd (5'-amino-5'-deoxythymidine), a study of the HSV-1 DNA synthesized in infected Vero cells exposed to 5'-AdThd was undertaken. Unlike many other antiviral nucleoside analogs, 5'-AdThd did not inhibit HSV-1 DNA synthesis. Analysis of t...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(84)90032-9
更新日期：1984-10-01 00:00:00
abstract：:Cyclosporin A (CsA) was assessed for its effect on the production of antiviral activity by human peripheral blood leukocytes (PBL). CsA markedly reduced the production of interferon-gamma (IFN-gamma) in response to stimulation with lectin mitogens, bacterial products, alloantigens, or Epstein-Barr virus (EBV)-transfor...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(82)90006-7
更新日期：1982-12-01 00:00:00
abstract：:Two new influenza virus neuraminidase inhibitors (NAIs), peramivir and laninamivir, were approved in 2010 which resulted to four NAIs that were used during the 2010-2011 influenza season in Japan. This study aims to monitor the susceptibility of influenza virus isolates in 2009-2010 and 2010-2011 influenza seasons in ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2013.06.003
更新日期：2013-09-01 00:00:00
abstract：:Investments in the development of new drugs for orthopoxvirus infections have fostered new avenues of research, provided an improved understanding of orthopoxvirus biology and yielded new therapies that are currently progressing through clinical trials. These broad-based efforts have also resulted in the identificatio...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2012.02.012
更新日期：2012-05-01 00:00:00
abstract：:Systemic treatment of mice with (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) showed a significant therapeutic efficacy against herpes simplex type 1 virus (HSV-1) encephalitis. With treatment initiated 12 h after viral inoculation and continued for 10 consecutive days, BVDU administered intraperitoneally in daily doses...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(83)90010-4
更新日期：1983-03-01 00:00:00
abstract：:The viral genotype and serum viral level influence the response to interferon (IFN) treatment in patients with chronic hepatitis C virus (HCV) viremia. The aim of this study was to investigate a possible relationship between early virological response and helper T (Th) cell cytokine expansion by 4 weeks of ribavirin (...
journal_title：Antiviral research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.antiviral.2005.04.001
更新日期：2005-07-01 00:00:00
abstract：:Carbovir (CBV) is a guanine nucleoside analog with potent in vitro anti-HIV activity. A prodrug of CBV is currently being evaluated in clinical trials as a potential agent for the treatment of AIDS. The ability of CBV to inhibit mitochondrial DNA synthesis in intact CEM cells was evaluated in the present study, becaus...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(97)01033-4
更新日期：1997-05-01 00:00:00
abstract：:Dolutegravir (S/GSK1349572) is a second-generation HIV-1 integrase inhibitor (INI) in advanced clinical development. It has shown good antiviral activity in most patients with prior raltegravir failure, although changes at the integrase codon 148, particularly when combined with other mutations, confer reduced suscept...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2011.03.178
更新日期：2011-06-01 00:00:00
abstract：:Infection with hepatitis B virus (HBV) or hepatitis C virus (HCV) may result in a number of different clinical outcomes. There is strong evidence in HBV infection that host genetic factors play a major role in determining the outcome of infection. A number of approaches may be used to determine the specific genetic fa...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/s0166-3542(01)00175-9
更新日期：2001-11-01 00:00:00
abstract：BACKGROUND:By modulating the antiviral immune response via vitamin D receptor, the active form of vitamin D (1,25-dihydroxyvitamin D, calcitriol) could play a central role in protection against respiratory virus infections. This in vitro study tested the hypothesis that respiratory viruses modulate vitamin D receptor e...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.11.004
更新日期：2017-01-01 00:00:00
abstract：:Varicella-zoster virus (VZV) replicates in quiescent T cells, neurons, and skin cells. In cultured fibroblasts (HFFs), VZV induces host cyclin expression and cyclin-dependent kinase (CDK) activity without causing cell cycle progression. CDK1/cyclin B1 phosphorylates the major viral transactivator, and the CDK inhibito...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2010.03.007
更新日期：2010-06-01 00:00:00
abstract：:Approximately 170 million people worldwide are chronic carriers of Hepatitis C virus (HCV). To date, there is no prophylactic vaccine available against HCV. The standard-of-care therapy for HCV infection involves a combination of pegylated interferon-α and ribavirin. This therapy, which is commonly associated with sid...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2012.07.006
更新日期：2012-10-01 00:00:00
abstract：:The era of antiviral chemotherapy started more than 50 years with the findings by Domagk and his colleagues that thiosemicarbazones showed activity against vaccinia virus. One of the derivatives, methisazone, was even investigated in the prophylaxis of smallpox. With the successful implementation of the smallpox vacci...
journal_title：Antiviral research
pub_type： 历史文章,杂志文章,评审
doi：10.1016/s0166-3542(02)00197-3
更新日期：2003-01-01 00:00:00
abstract：:Epidermal growth factor receptor (EGFr) has been shown to be induced and activated in cells infected with HPV, suggesting that it may play a physiological role in viral replication or in the formation or maintenance of warts. To investigate this possibility, human foreskin tissue was infected with HPV11 and transplant...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.12.004
更新日期：2007-04-01 00:00:00
abstract：:Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA), the stable genomic form as the template for viral transcription, plays a crucial role in viral persistence which remains a major global health problem. While accumulating evidence suggests the involvement of transcription factors and epigenetic machinery...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2018.03.008
更新日期：2018-05-01 00:00:00
abstract：:To compare incidence, risk factors and morphologic pattern of hepatocellular carcinoma (HCC) development in hepatitis B virus (HBV) and hepatitis C virus (HCV) related cirrhosis, 401 patients were followed prospectively by periodic ultrasound examination for 14-189 months (mean: 84.8+/-36.7). During follow-up, 77 (19....
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(01)00185-1
更新日期：2001-11-01 00:00:00
abstract：:Desferrioxamine (DFO) is commonly used in therapy as a chelator of ferric ion in disorders of iron overload. We found that DFO inhibits human cytomegalovirus (HCMV) replication in infected cultures of human foreskin fibroblasts (HFF) at concentrations that have been achieved in humans with no significant adverse effec...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(94)90095-7
更新日期：1994-09-01 00:00:00
abstract：:Prostaglandins are potentially useful antiviral agents, however their mechanism of action is unclear. Recent evidence suggests that RNA transcription of vesicular stomatitis virus (VSV) is inhibited by prostaglandins (Bader and Ankel, J. Gen. Virol. 71, 2823-2832, 1990). Prostaglandins are known to have multiple effec...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(94)00067-i
更新日期：1995-01-01 00:00:00
abstract：:(S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine, HPMPC, and two HPMPC-related nucleoside analogs, (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine, HPMPA, and (2-phosphonylmethoxyethyl)guanine, PMEG, were evaluated for their antiviral activities against guinea pig cytomegalovirus (GPCMV) infection in guinea pig ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(90)90069-j
更新日期：1990-05-01 00:00:00
abstract：:Avian influenza A(H6N1) virus is one of the most common viruses isolated from migrating birds and domestic poultry in many countries. The first and only known case of human infection by H6N1 virus in the world was reported in Taiwan in 2013. This led to concern that H6N1 virus may cause a threat to public health. In t...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2015.10.019
更新日期：2016-02-01 00:00:00
abstract：:Human cytomegalovirus terminase complex cleaves the concatemeric genomic viral DNA into unit lengths during genome packaging and particle assembly. Terminase complex ATPase and endonuclease activity is provided by the viral protein pUL89. pUL89 is an attractive drug target because its activities are required for infec...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2018.01.015
更新日期：2018-04-01 00:00:00
abstract：:Cellular immune responses (T cell responses) during hepatitis C virus (HCV) infection are significant factors for determining the outcome of infection. HCV adapts to host immune responses by inducing mutations in its genome at specific sites that are important for HLA processing/presentation. Moreover, HCV also adapts...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.10.013
更新日期：2017-01-01 00:00:00
abstract：:Prunellin, an anti-HIV active compound, was isolated from aqueous extracts of the Chinese medicinal herb, Prunella vulgaris, and purified to chromatographic homogeneity. Infrared and NMR spectroscopy identified prunellin as a polysaccharide. Elemental analyses, precipitation with calcium(II), barium(II), or 9-aminoacr...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(89)90036-3
更新日期：1989-06-01 00:00:00
