Abstract：
:The heterocycle, 4,6-dibenzamidopyrazolo[3,4-d]pyrimidine (DBAPP), inhibited cytopathology induced by human, mouse, and vervet monkey cytomegaloviruses (CMV) in vitro at 0.2 to 0.5 microM, but did not inhibit cell replication at less than or equal to 30 microM. Herpes simplex viruses were unaffected by the inhibitor. The antiviral agent ganciclovir was effective against these CMVs at 3-10 microM in parallel assays. DBAPP and ganciclovir were synergistic inhibitors when used in combination. The heterocycle was only active if applied to cells before virus replication, indicating that it inhibited virus adsorption. Cells pre-treated 1 h with 30 microM DBAPP, then extensively rinsed, were resistant to infection by mouse CMV even 3 days after removal of the inhibitor. Human and monkey CMVs were able to infect cells and replicate within 24 h of drug removal. When virus and DBAPP were combined together then dialyzed to remove the compound, mouse CMV infectivity was decreased 1.7 logs, whereas human CMV and monkey CMV infectivity titers were relatively unaffected. Treatment of mice with DBAPP twice a day for 7 days starting 6 h after mouse CMV inoculation caused a moderate increase in number of survivors at 30 mg/kg. Cell to cell spread of the virus may account for poor efficacy of the compound when added after virus infection. DBAPP may serve as a tool to explore aspects of CMV adsorption or to characterize the cellular component of the CMV receptor.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Smee DF,Bartlett ML,Alaghamandan HA,Jones MM,Revankar GR,Robins RKdoi
10.1016/0166-3542(90)90040-esubject
Has Abstractpub_date
1990-01-01 00:00:00pages
1-10issue
1eissn
0166-3542issn
1872-9096pii
0166-3542(90)90040-Ejournal_volume
13pub_type
杂志文章abstract：:Many flaviviruses, such as Zika virus (ZIKV), Dengue virus (DENV1-4) and yellow fever virus (YFV), are significant human pathogens. Infection with ZIKV, an emerging mosquito-borne flavivirus, is associated with increased risk of microcephaly in newborns and Guillain-Barré syndrome and other complications in adults. Cu...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2017.12.018
更新日期：2018-02-01 00:00:00
abstract：:The reverse transcriptase (RT) of HIV which has been inhibited by the incorporation of AZT into the primer strand is subject to a deblocking reaction by cellular ATP. This reaction yields unblocked primer plus the dinucleoside tetraphosphate, AZTp(4)A. In the present study, we report that AZTp(4)A is an excellent subs...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(03)00003-2
更新日期：2003-05-01 00:00:00
abstract：:This study aimed to investigate anti-HBV effect and major active compounds of Su-duxing, a medicine extracted from Chinese herbs. HBV-replicating cell lines HepG2.2.15 (wild-type) and HepG2.A64 (entecavir-resistant) were used for in vitro test. C57BL/6 mice infected by adeno-associated virus carrying 1.3 mer wild-type...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2018.04.017
更新日期：2018-07-01 00:00:00
abstract：:Forty-nine subjects were enrolled in a study comparing two dosages of parenterally administered interferon (IFN)-beta in combination with cryotherapy for the treatment of anogenital warts. Subjects were randomized to receive subcutaneous injections of either 2 x 10(6) or 4 x 10(6) IU/m2 of IFN-beta (Biogen) three time...
journal_title：Antiviral research
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0166-3542(97)01037-1
更新日期：1997-06-01 00:00:00
abstract：:We isolated a ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) from a laboratory strain, AD169, and analysed the mutant. Attempts were also made to identify directly the mutated gene. The 50% inhibitory concentration (IC50) of GCV for the mutant strain was five times higher than that of the wild-type strain. T...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(98)00051-5
更新日期：1998-12-01 00:00:00
abstract：:A severe inflammatory immune response with hypercytokinemia occurs in patients hospitalized with severe influenza, such as avian influenza A(H5N1), A(H7N9), and seasonal A(H1N1)pdm09 virus infections. The role of immunomodulatory therapy is unclear as there have been limited published data based on randomized controll...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2018.01.002
更新日期：2018-02-01 00:00:00
abstract：:JNJ63623872 (formerly known as VX-787) is an inhibitor of influenza A virus polymerases through interaction with the viral PB2 subunit. This interaction blocks the cap-snatching activity of the virus that is essential for virus replication. Previously published work has documented antiviral activity of JNJ63623872 in ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.10.009
更新日期：2016-12-01 00:00:00
abstract：:The chemically modified DNA, apurinic acid (APA), is cytotoxic for human lymphocytes at concentrations above 100 micrograms/ml. At low concentrations (0.05-1 micrograms/ml) APA acts as an inducer interferon gamma (IFN-gamma) in lymphocytes in vitro; the maximum interferon titer of 50 units/ml was reached at 0.4 microg...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(88)90003-4
更新日期：1988-04-01 00:00:00
abstract：DESIGN:The HIV latent CD4+ T cell reservoir is broadly recognized as a barrier to HIV cure. Induction of HIV expression using protein kinase C (PKC) agonists is one approach under investigation for reactivation of latently infected CD4+ T cells (Beans et al., 2013; Abreu et al., 2014; Jiang et al., 2014; Jiang and Dand...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.11.014
更新日期：2017-03-01 00:00:00
abstract：:Cellular immune responses (T cell responses) during hepatitis C virus (HCV) infection are significant factors for determining the outcome of infection. HCV adapts to host immune responses by inducing mutations in its genome at specific sites that are important for HLA processing/presentation. Moreover, HCV also adapts...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.10.013
更新日期：2017-01-01 00:00:00
abstract：UNLABELLED:This paper presents the public health rationale for multipurpose prevention technologies (MPTs) for sexual and reproductive health (SRH) based on regional trends in demographic and SRH indicators. It then distils important lessons gleaned from the introduction of contraceptive and reproductive health product...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2013.09.019
更新日期：2013-12-01 00:00:00
abstract：:Infection of captive macaques with simian immunodeficiency virus (SIV) and domestic cats with feline immunodeficiency virus (FIV), both discovered in the last five years, represent excellent animal models for infection of humans with the human immunodeficiency virus (HIV). Protection against challenge infection and pr...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/0166-3542(91)90009-g
更新日期：1991-05-01 00:00:00
abstract：:Although alpha interferon (IFN-alpha) is of benefit in the treatment of viral hepatitis B, HBV replication has been refractory to the cytokine in commonly used hepatocyte-derived cell lines. In search for a cell culture system to study the mechanism by which IFN-alpha inhibits HBV replication, we infected a variety of...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2007.06.015
更新日期：2007-12-01 00:00:00
abstract：:We studied susceptibility to herpes labialis by exposing 20 volunteers to experimental ultraviolet radiation (UVR) on three occasions at 3- to 4-month intervals. The number of patients who developed lesions after each session was 9/20 (45%), 9/20 (45%) and 14/20 (70%). Herpes simplex virus (HSV) was isolated from 21/2...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(95)00038-n
更新日期：1995-09-01 00:00:00
abstract：:The latent reservoir of HIV-1 in resting memory CD4+ T cells serves as a major barrier to curing HIV-1 infection. Reactivation of latent HIV-1 is proposed as a promising strategy for the clearance of the viral reservoirs. Because of the limitations of current latency reversal agents (LRAs), identification of new LRAs ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2019.104555
更新日期：2019-09-01 00:00:00
abstract：:A series of four mannose(Man)-, three N-acetylglucosamine (GlcNAc)n-, ten N-acetylgalactosamine/galactose(GalNAc/Gal)-, one 5-acetylneuraminic acid (alpha-2,3-Gal/GalNAc)- and one 5-acetylneuroaminic acid(alpha-2,6-Gal/Gal-NAc)-specific plant agglutinins were evaluated for their antiviral activity in vitro. the mannos...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(92)90038-7
更新日期：1992-06-01 00:00:00
abstract：:Current therapies for chronic hepatitis B virus infection (CHB) - nucleos(t)ide analogue reverse transcriptase inhibitors and interferons - result in low rates of functional cure defined as sustained off-therapy seroclearance of hepatitis B surface antigen (HBsAg). One likely reason is the inability of these therapies...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2015.06.019
更新日期：2015-09-01 00:00:00
abstract：:The study aimed at identifying biomarkers of immune response elicited by non-adjuvanted-(NAV) and adjuvanted-(AV) H1N1(pdm09) vaccines. The results showed that despite both vaccines elicited similar levels of anti-H1N1 antibodies at day30 after vaccination, higher reactivity was observed in AV at day180. While AV indu...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2017.05.009
更新日期：2017-08-01 00:00:00
abstract：:Tiazofurin (2-beta-D-ribofuranosylthiazole-4-carboxamide) was examined for its activity against tomato-spotted wilt virus (TSWV) in tomato plants. Solutions containing 50, 100, 200, 400 and 800 mg/l of the drug were sprayed onto the leaves. The results showed that 100 and 200 mg/l were the most efficient concentration...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(84)90003-2
更新日期：1984-12-01 00:00:00
abstract：:In an ongoing effort to identify an orally bioavailable compound for the treatment of rhino- and enteroviral infections, a series of vinylacetylene benzimidazoles was recently examined. Previous studies demonstrated the potential for these compounds to possess both good in vitro antiviral activity as well as acceptabl...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(99)00013-3
更新日期：1999-05-01 00:00:00
abstract：:Efavirenz (EFV), a first generation non-nucleoside analogue reverse transcriptase inhibitor widely employed in combination antiretroviral therapy regimens over the last 20 years, has been associated with a wide range of neuropsychiatric effects and has also been linked with HIV-associated neurocognitive disorder (HAND...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2019.04.015
更新日期：2019-08-01 00:00:00
abstract：:The model of simian immunodeficiency virus (SIV) infection in rhesus macaques was used to evaluate the effects of recombinant human interferon alpha, Hu IFN-alpha 2b and Hu IFN-gamma B,D, at two doses. Administration began 1 day prior to infection and was continued for 90 days postinfection. Both interferons suppresse...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(95)00955-8
更新日期：1996-08-01 00:00:00
abstract：:Although canine rabies has been eliminated from industrialized countries, infected dogs remain the primary source of human and livestock exposures in Asia, Africa and much of South America. Human deaths are the most important direct economic impact of canine rabies, followed by livestock losses and the cost of PEP, wh...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2013.03.004
更新日期：2013-05-01 00:00:00
abstract：:Cytomegalovirus (CMV) is the most common cause of congenital infection in the developed world and can lead to a life-threatening disease. We therefore developed an animal model to evaluate candidate anti-CMV drugs and to further define the pathogenesis of CMV infections. Newborn guinea pigs were infected by intraperit...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(03)00151-7
更新日期：2003-09-01 00:00:00
abstract：:Site-directed in vitro mutagenesis was used to create analogs of human interferons (IFNs)-alpha 1 and -alpha 4. Analogs were expressed in vitro using SP6 RNA polymerase and a rabbit reticulocyte lysate cell-free protein synthesis system. Amino acid substitutions for the highly conserved residues at positions 33, 121, ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(89)90066-1
更新日期：1989-08-01 00:00:00
abstract：:The TC-83 vaccine strain of Venezuelan equine encephalitis virus (VEEV) causes encephalitis and death in C3H/HeN mice infected by intranasal (i.n.) instillation. Since TC-83 is exempt as a select agent, this mouse model was used in the evaluation of antiviral therapies. Virus titers in the brains of infected mice peak...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2008.01.007
更新日期：2008-06-01 00:00:00
abstract：:There is a pressing need for new vaccines against alphaviruses, which can cause fatal encephalitis (Venezuelan equine encephalitis virus (VEEV) and others) and severe arthralgia (e.g. Chikungunya virus, CHIKV). These positive-strand RNA viruses are diverse and evolve rapidly, meaning that the sequence of any vaccine s...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104905
更新日期：2020-10-01 00:00:00
abstract：:The present study was undertaken to examine the potential of rectal route of immunization for induction of protective immunity in the female genital tract against genital herpes infection in mice. A single rectal immunization of female C57Bl/6 mice with live attenuated herpes simplex virus type 2 lacking thymidine kin...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2007.12.014
更新日期：2008-06-01 00:00:00
abstract：:We studied the effects of two antiviral agents, human interferon-alpha (IFN-alpha) and ribavirin, on subacute sclerosing panencephalitis (SSPE) virus infections in hamsters. By intracranial administration, IFN-alpha alone improved the survival of infected hamsters by 20% at a dose of 6 x 10(4) IU/kg every other day fo...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(97)00055-7
更新日期：1998-01-01 00:00:00
abstract：:Acute lower respiratory tract infections (ALRI) caused by respiratory syncytial virus (RSV) are associated with a severe disease burden among infants and elderly patients. Treatment options are limited. While numerous drug candidates with different viral targets are under development, the utility of RSV entry inhibito...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104774
更新日期：2020-05-01 00:00:00
