Abstract：
:The reverse transcriptase (RT) of HIV which has been inhibited by the incorporation of AZT into the primer strand is subject to a deblocking reaction by cellular ATP. This reaction yields unblocked primer plus the dinucleoside tetraphosphate, AZTp(4)A. In the present study, we report that AZTp(4)A is an excellent substrate for the enzyme Ap(4)A hydrolase (asymmetrical dinucleoside tetraphosphatase, EC 3.6.1.17), an enzyme that is widely distributed in many cell types. Progress of the reaction has been monitored by 31P NMR, and it was found that hydrolysis results in the production of AZTTP:ATP in a 7:1 ratio. The AZTp(4)A was also hydrolyzed at a rate 1.8-fold more rapidly than Ap(4)A. Spectrophotometric assays yielded Michaelis constants of 2.35 and 0.71 microM for Ap(4)A and AZTp(4)A, respectively. It, therefore, appears that Ap(4)A hydrolase can play a useful role in the regeneration of the AZTTP, the active form of AZT, for the inhibition of HIV RT.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Pitcher WH 3rd,Kirby TW,DeRose EF,London REdoi
10.1016/s0166-3542(03)00003-2subject
Has Abstractpub_date
2003-05-01 00:00:00pages
227-33issue
3eissn
0166-3542issn
1872-9096pii
S0166354203000032journal_volume
58pub_type
杂志文章abstract：:The hepatitis B virus nucleocapsid or core antigen is extremely immunogenic during infection and after immunization. This review summarizes several features of the nucleocapsid which explain this exceptionally high immunogenicity: a unique three-dimensional folding, the presence of a region that interacts with immunog...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2003.08.011
更新日期：2003-10-01 00:00:00
abstract：:Prostaglandins are potentially useful antiviral agents, however their mechanism of action is unclear. Recent evidence suggests that RNA transcription of vesicular stomatitis virus (VSV) is inhibited by prostaglandins (Bader and Ankel, J. Gen. Virol. 71, 2823-2832, 1990). Prostaglandins are known to have multiple effec...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(94)00067-i
更新日期：1995-01-01 00:00:00
abstract：BACKGROUND:Cases of sustained-virological response (SVR or cure) after an ultra-short duration (≤27 days) of direct-acting antiviral (DAA)-based therapy, despite HCV being detected at end of treatment (EOT), have been reported. Established HCV mathematical models that predict the treatment duration required to achieve ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2017.06.019
更新日期：2017-08-01 00:00:00
abstract：:Coumarins and structurally related compounds have been recently shown to inhibit replication of human immunodeficiency virus (HIV) and thus, exhibit a therapeutic potential. In this study we report that mesuol and isomesuol, two 4-phenyl coumarins, isolated from the tree Marila pluricostata, suppress HIV-1 replication...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2005.02.006
更新日期：2005-06-01 00:00:00
abstract：:The emergence of multidrug-resistant influenza viruses poses a persistent threat to public health. The current prophylaxis and therapeutic interventions for influenza virus infection have limited efficacy due to the continuous antigenic drift and antigenic shift of influenza viruses. As part of our ongoing effort to d...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2017.07.016
更新日期：2017-09-01 00:00:00
abstract：:During studies of the antiviral activity of chalcone Ro-09-0410 on human rhinovirus type 9 (RV9) chalcone-resistant strains of RV9 were isolated and appeared with a frequency of about 10(-5) in chalcone sensitive stock. Chalcone-dependent viruses were found after further passage. Some characteristics of the resistant ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(87)90085-4
更新日期：1987-08-01 00:00:00
abstract：:Commercial vaccines against human papillomavirus (HPV) have low uptake due to parental autonomy, dosing regimen, cost, and cold chain storage requirements. Carrageenan (CG)-based formulations prevent HPV infection in vitro and in vivo but data are needed on the durability of anti-HPV activity and the effect of seminal...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2014.05.018
更新日期：2014-08-01 00:00:00
abstract：BACKGROUND:By modulating the antiviral immune response via vitamin D receptor, the active form of vitamin D (1,25-dihydroxyvitamin D, calcitriol) could play a central role in protection against respiratory virus infections. This in vitro study tested the hypothesis that respiratory viruses modulate vitamin D receptor e...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.11.004
更新日期：2017-01-01 00:00:00
abstract：:There is a pressing need for new vaccines against alphaviruses, which can cause fatal encephalitis (Venezuelan equine encephalitis virus (VEEV) and others) and severe arthralgia (e.g. Chikungunya virus, CHIKV). These positive-strand RNA viruses are diverse and evolve rapidly, meaning that the sequence of any vaccine s...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104905
更新日期：2020-10-01 00:00:00
abstract：:The prevention and control of disease caused by seasonal and potential pandemic influenza viruses is currently managed by the use influenza vaccines and antivirals. The adamantanes (amantadine and rimantadine) were the first antivirals licensed for use against influenza A viruses and have been used extensively in some...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.08.002
更新日期：2007-02-01 00:00:00
abstract：:Prunellin, an anti-HIV active compound, was isolated from aqueous extracts of the Chinese medicinal herb, Prunella vulgaris, and purified to chromatographic homogeneity. Infrared and NMR spectroscopy identified prunellin as a polysaccharide. Elemental analyses, precipitation with calcium(II), barium(II), or 9-aminoacr...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(89)90036-3
更新日期：1989-06-01 00:00:00
abstract：UNLABELLED:This paper presents the public health rationale for multipurpose prevention technologies (MPTs) for sexual and reproductive health (SRH) based on regional trends in demographic and SRH indicators. It then distils important lessons gleaned from the introduction of contraceptive and reproductive health product...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2013.09.019
更新日期：2013-12-01 00:00:00
abstract：:Laninamivir, a potent neuraminidase (NA) inhibitor, is an active metabolite of laninamivir octanoate (code name: CS-8958) which is a long acting NA inhibitor and is commercially available under the brand name Inavir in Japan to complete the treatment of influenza by a single inhalation. It is supposed that the long ac...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2013.08.004
更新日期：2013-10-01 00:00:00
abstract：:The antiviral effect of foscarnet (PFA) on the replication of rotavirus, a member of the Reoviridae, was studied. The pyrophosphate analogue is an effective inhibitor of several viral polymerases acting on the enzyme pyrophosphate binding site. Replication of rotavirus in MA104 cells using different u.o.i. was inhibit...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(94)00085-m
更新日期：1995-05-01 00:00:00
abstract：:Global eradication of hepatitis C virus (HCV) infection will require an efficacious vaccine capable of eliciting protective immunity against genetically diverse HCV strains. Natural spontaneous resolution of HCV infection is associated with production of broadly-neutralizing antibodies targeting the HCV glycoproteins ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2018.09.005
更新日期：2018-12-01 00:00:00
abstract：:Hepatitis C virus (HCV) is a leading cause of chronic hepatitis and end-stage liver diseases. Mature HCV virions are bound by host-derived lipoproteins. Lack of an HCV vaccine warrants a major role of antiviral treatment in the global elimination of hepatitis C. Although direct-acting antivirals (DAAs) are replacing t...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104734
更新日期：2020-05-01 00:00:00
abstract：:On September 22, 2008, a physician on Prince of Wales Island, Alaska, notified the Alaska Department of Health and Social Services (ADHSS) of an unusually high number of adult patients with recently diagnosed pneumonia (n = 10), including three persons who required hospitalization and one who died. ADHSS and CDC condu...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2018.08.004
更新日期：2018-10-01 00:00:00
abstract：:Nipah and Hendra virus are members of a newly identified genus of emerging paramyxoviruses, the henipaviruses. Both viruses have the ability to cause severe pulmonary infection and severe acute encephalitis. Following their discovery in the 1990s, outbreaks caused by these zoonotic paramyxoviruses have been associated...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2012.05.008
更新日期：2012-08-01 00:00:00
abstract：:Human papilloma virus (HPV)-16 is the prevalent genotype associated with cervical tumours. Virus-like-particle (VLP)-based vaccines have proven to be effective in limiting new infections of high-risk HPVs, but their high cost has hampered their use, especially in the poor developing countries. Avipox-based recombinant...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2015.01.012
更新日期：2015-04-01 00:00:00
abstract：OBJECTIVE:We sought to investigate the differences in monocyte immune responses to the dengue virus (DENV) in those who previously had either severe disease (past SD) or non-severe dengue (past NSD) following a secondary dengue infection. METHOD:Monocytes from healthy individuals who had either past SD (n = 6) or past...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2019.104554
更新日期：2019-09-01 00:00:00
abstract：:Infections by dengue virus (DENV) are increasing worldwide, with an urgent need for effective anti-DENV agents. We recently identified N-(4-hydroxyphenyl) retinamide (4-HPR), an anti-DENV agent effective against all 4 serotypes of DENV in cell culture, and in a lethal mouse model for DENV infection (Fraser et al., 201...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2016.03.006
更新日期：2016-06-01 00:00:00
abstract：:The influenza A virus (IAV) NS1 protein is one of the major regulators of pathogenicity, being able to suppress innate immune response and host protein synthesis. In this study we identified the human micro RNA hsa-miR-1307-3p as a novel potent suppressor of NS1 expression and influenza virus replication. Transcriptom...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2019.104593
更新日期：2019-11-01 00:00:00
abstract：:While the combination of zidovudine and didanosine is used in HIV-infected patients with more advanced disease, the possibility of a pharmacokinetic interaction between these two drugs remains controversial. Zidovudine doses of 50, 100, and 200 mg, combined with 67, 167, and 250 mg of didanosine were evaluated in 11 a...
journal_title：Antiviral research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0166-3542(95)00032-h
更新日期：1995-08-01 00:00:00
abstract：:Interferon alpha-2b (IFN) alone or in combination with Ribavirin is approved in the United States for the treatment of chronic hepatitis C virus (HCV) infection. We have previously reported that the glucosidase inhibitor, n-butyl deoxynojirimycin (nB-DNJ) inhibits the production of infectious bovine diarrhea virus (BV...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(02)00075-x
更新日期：2002-09-01 00:00:00
abstract：:The viral genotype and serum viral level influence the response to interferon (IFN) treatment in patients with chronic hepatitis C virus (HCV) viremia. The aim of this study was to investigate a possible relationship between early virological response and helper T (Th) cell cytokine expansion by 4 weeks of ribavirin (...
journal_title：Antiviral research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.antiviral.2005.04.001
更新日期：2005-07-01 00:00:00
abstract：:Infectious bursal disease (IBD) leads to considerable economic losses for the poultry industry by inducing severe immunosuppression and high mortality in chickens. The objective of this study was to determine if RNA interference (RNAi) could be utilized to inhibit IBDV replication in vitro. We selected 3 short interfe...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2007.11.007
更新日期：2008-08-01 00:00:00
abstract：:The ability to control plant viral diseases with chemicals has great potential value for agriculture, but few chemicals are available to date due to the difficulty in obtaining effective drugs. IMP dehydrogenase is an enzyme which catalyzes the conversion of inosine 5'-monophosphate to xanthosine 5'-monophosphate in t...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.10.007
更新日期：2007-03-01 00:00:00
abstract：:The era of antiviral chemotherapy started more than 50 years with the findings by Domagk and his colleagues that thiosemicarbazones showed activity against vaccinia virus. One of the derivatives, methisazone, was even investigated in the prophylaxis of smallpox. With the successful implementation of the smallpox vacci...
journal_title：Antiviral research
pub_type： 历史文章,杂志文章,评审
doi：10.1016/s0166-3542(02)00197-3
更新日期：2003-01-01 00:00:00
abstract：:The introduction of potent combination therapies in the mid-90s had a tremendous effect on AIDS mortality. However, drug resistance has been a major factor contributing to antiretroviral therapy failure. Currently, there are 26 drugs approved for treating human immunodeficiency virus (HIV) infections, although some of...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2013.01.007
更新日期：2013-04-01 00:00:00
abstract：:An extract of the cactus plant Opuntia streptacantha inhibited intracellular virus replication and inactivated extracellular virus. Inhibition of virus replication also occurred following pre-infection treatment--a favourable finding in terms of in-vivo limitation of virus disease. There was inhibition of both DNA and...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(95)00839-x
更新日期：1996-05-01 00:00:00
