Abstract：
:There is a pressing need for new vaccines against alphaviruses, which can cause fatal encephalitis (Venezuelan equine encephalitis virus (VEEV) and others) and severe arthralgia (e.g. Chikungunya virus, CHIKV). These positive-strand RNA viruses are diverse and evolve rapidly, meaning that the sequence of any vaccine should cover multiple strains that may be quite different from any previous isolate. Here, consensus proteins were produced to represent the common physicochemical properties (PCPs) of the epitope rich, B domain of the E2 envelope protein. PCP-consensus proteins were based on multiple strains of VEEV (VEEVcon) and CHIKV (CHIKVcon) or the conserved PCPs of 24 different alphaviruses (AllAVcon). The AllAVcon was altered to include binding sites for neutralizing antibodies of both VEEV and CHIKV strains (Mosaikcon). All four designed proteins were produced solubly in E. coli and purified. They formed the β-strand core expected from experimental structures of this region of the wild type E2 proteins as indicated by circular dichroism (CD) spectra. Furthermore, the CHIKVcon protein bound to a structure dependent, CHIKV neutralizing monoclonal antibody. The AllAVcon and Mosaikcon proteins bound to polyclonal antibodies generated during natural infection with either VEEV or CHIKV, indicating they contained epitopes of both serotypes. The Mosaikcon antigen induced antibodies in rabbit sera that recognized both the VEEVcon and CHIKVcon spike proteins. These PCP-consensus antigens are promising starting points for novel, broad-spectrum alphavirus vaccines.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Baker WS,Negi S,Braun W,Schein CHdoi
10.1016/j.antiviral.2020.104905subject
Has Abstractpub_date
2020-10-01 00:00:00pages
104905eissn
0166-3542issn
1872-9096pii
S0166-3542(20)30319-3journal_volume
182pub_type
杂志文章abstract：:Distamycin-A hydrochloride, a synthetic antibiotic, and 2,3-dihydroxy-6-bromo-pyrazino (2,3-beta) pyrazine derivative, were used against tomato spotted wilt virus (TSWV) in tobacco plants. The drugs were applied to the leaves at concentrations of 200 and 400 mg/l. The results showed that both drugs delayed virus sprea...
journal_title：Antiviral research
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：1997-05-01 00:00:00
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journal_title：Antiviral research
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更新日期：1992-07-01 00:00:00
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journal_title：Antiviral research
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
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更新日期：1997-06-01 00:00:00
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pub_type： 杂志文章
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journal_title：Antiviral research
pub_type： 杂志文章
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journal_title：Antiviral research
pub_type： 杂志文章
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2007-04-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2012-07-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2018-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2018-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(98)00015-1
更新日期：1998-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：1997-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：2010-11-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2004.12.007
更新日期：2005-04-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(92)90086-k
更新日期：1992-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2014-12-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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journal_title：Antiviral research
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journal_title：Antiviral research
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2018-04-01 00:00:00
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journal_title：Antiviral research
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更新日期：2019-08-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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journal_title：Antiviral research
pub_type： 杂志文章
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2012-09-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
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更新日期：2017-07-01 00:00:00
