当前位置： SCI文献检索 > ANTIVIRAL RESEARCH期刊下所有文献 > A quantitative method for hepatitis B virus covalently closed circular DNA enables distinguishing direct acting antivirals from cytotoxic agents.

A quantitative method for hepatitis B virus covalently closed circular DNA enables distinguishing direct acting antivirals from cytotoxic agents.

Abstract：

:Studying the biogenesis of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) and developing anti-HBV agents require analytical methods to quantify viral DNA levels inside host cells. The well-accepted Southern blotting method is only semi-quantitative, while the other widely used methods (based on qPCR) have been questioned as to their fidelity for cccDNA quantification. In addition, Southern blotting and qPCR results barely reflect the number of host cells present in an analytical sample. We here developed new techniques that substantially improve cccDNA detection and quantification, including a sample pretreatment method that i) exploits high temperature and exonuclease V (an ATP-dependent, bidirectional exonuclease) digestion to effectively increase the amplification efficiency of HBV cccDNA by removing rcDNA and denaturing the cccDNA template, and ii) a method that splits cell samples and uses separate extraction technologies to facilitate "host normalization" based on host genomic DNA signals. Our study introduces new analytical techniques that should be useful for the basic biology and translational studies of HBV.

journal_name

Antiviral Res

journal_title

Antiviral research

authors

Gao Z,Yan L,Li W

doi

10.1016/j.antiviral.2019.06.002

subject

Has Abstract

pub_date

2019-08-01 00:00:00

pages

197-202

eissn

0166-3542

issn

1872-9096

pii

S0166-3542(19)30242-6

journal_volume

168

pub_type

杂志文章

相关文献

ANTIVIRAL RESEARCH文献大全
  • Suramin inhibits Zika virus replication by interfering with virus attachment and release of infectious particles.

    abstract：:Zika virus (ZIKV) is a mosquito-borne flavivirus that mostly causes asymptomatic infections or mild disease characterized by low-grade fever, rash, conjunctivitis, and malaise. However, the recent massive ZIKV epidemics in the Americas have also linked ZIKV infection to fetal malformations like microcephaly and Guilla...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2017.04.016

    authors： Albulescu IC,Kovacikova K,Tas A,Snijder EJ,van Hemert MJ

    更新日期：2017-07-01 00:00:00

  • In vitro anti-influenza A activity of interferon (IFN)-λ1 combined with IFN-β or oseltamivir carboxylate.

    abstract：:Influenza viruses, which can cross species barriers and adapt to new hosts, pose a constant potential threat to human health. The influenza pandemic of 2009 highlighted the rapidity with which an influenza virus can spread worldwide. Currently available antivirals have a number of limitations against influenza, and no...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2014.09.008

    authors： Ilyushina NA,Donnelly RP

    更新日期：2014-11-01 00:00:00

  • In vitro and in vivo evaluation of two carrageenan-based formulations to prevent HPV acquisition.

    abstract：:Commercial vaccines against human papillomavirus (HPV) have low uptake due to parental autonomy, dosing regimen, cost, and cold chain storage requirements. Carrageenan (CG)-based formulations prevent HPV infection in vitro and in vivo but data are needed on the durability of anti-HPV activity and the effect of seminal...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2014.05.018

    authors： Rodríguez A,Kleinbeck K,Mizenina O,Kizima L,Levendosky K,Jean-Pierre N,Villegas G,Ford BE,Cooney ML,Teleshova N,Robbiani M,Herold BC,Zydowsky T,Fernández Romero JA

    更新日期：2014-08-01 00:00:00

  • Emergence of resistance to acyclovir and penciclovir in varicella-zoster virus and genetic analysis of acyclovir-resistant variants.

    abstract：:We have characterized the differential actions of acyclovir and penciclovir against varicella-zoster virus (VZV) in cell culture by comparing the frequency of appearance of resistant viruses followed by their characterization. Cells were infected with cell-free virus and the cultures were successively treated with inc...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/s0166-3542(98)00057-6

    authors： Ida M,Kageyama S,Sato H,Kamiyama T,Yamamura J,Kurokawa M,Morohashi M,Shiraki K

    更新日期：1999-01-01 00:00:00

  • Cathelicidin antimicrobial peptides suppress EV71 infection via regulating antiviral response and inhibiting viral binding.

    abstract：:Cathelicidin antimicrobial peptides (human LL-37 and mouse CRAMP) are mainly virucidal to enveloped virus. However, the effects and relative mechanisms of LL-37 and CRAMP on non-enveloped virus are elusive. We herein found that CRAMP expression was significantly up-regulated post non-enveloped Enterovirus 71 (EV71) in...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2021.105021

    authors： Yu J,Dai Y,Fu Y,Wang K,Yang Y,Li M,Xu W,Wei L

    更新日期：2021-01-25 00:00:00

  • Persistent primary cytomegalovirus infection in a kidney transplant recipient: Multi-drug resistant and compartmentalized infection leading to graft loss.

    abstract：:Cytomegalovirus (CMV) is one of the most common opportunistic infections after transplantation. To prevent CMV infections, universal prophylaxis and pre-emptive therapy with ganciclovir or its prodrug valganciclovir is applied. However, prolonged antiviral therapy may result in drug-resistance emergence. We describe a...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2019.06.004

    authors： Andrei G,Van Loon E,Lerut E,Victoor J,Meijers B,Bammens B,Sprangers B,Gillemot S,Fiten P,Opdenakker G,Lagrou K,Kuypers D,Snoeck R,Naesens M

    更新日期：2019-08-01 00:00:00

  • Successful strategies implemented towards the elimination of canine rabies in the Western Hemisphere.

    abstract：:Almost all cases of human rabies result from dog bites, making the elimination of canine rabies a global priority. During recent decades, many countries in the Western Hemisphere have carried out large-scale dog vaccination campaigns, controlled their free-ranging dog populations and enforced legislation for responsib...

    journal_title：Antiviral research

    pub_type： 历史文章,杂志文章,评审

    doi：10.1016/j.antiviral.2017.03.023

    authors： Velasco-Villa A,Escobar LE,Sanchez A,Shi M,Streicker DG,Gallardo-Romero NF,Vargas-Pino F,Gutierrez-Cedillo V,Damon I,Emerson G

    更新日期：2017-07-01 00:00:00

  • Inhibition of reverse transcriptase activity of avian myeloblastosis virus by pyrophosphate analogues.

    abstract：:Several pyrophosphate analogues have been studied for their effects on avian myeloblastosis virus reverse transcriptase and on cellular DNA polymerase alpha. Examination of structure-activity relationships for these compounds revealed that two acidic groups connected by a short bridge were necessary, but not sufficien...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(82)90028-6

    authors： Eriksson B,Stening G,Oberg B

    更新日期：1982-05-01 00:00:00

  • Immunization with the transmembrane protein of a retrovirus, feline leukemia virus: absence of antigenemia following challenge.

    abstract：:A major challenge in the development of vaccines against retroviruses is the induction of neutralizing antibodies since they prevent infection of the cells where the virus may persist. The transmembrane envelope (TM) protein contains highly conserved domains and seems to be a suitable target. To study whether vaccinat...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2010.11.011

    authors： Langhammer S,Hübner J,Jarrett O,Kurth R,Denner J

    更新日期：2011-01-01 00:00:00

  • Therapeutic vaccination against chronic hepatitis C virus infection.

    abstract：:Approximately 170 million people worldwide are chronic carriers of Hepatitis C virus (HCV). To date, there is no prophylactic vaccine available against HCV. The standard-of-care therapy for HCV infection involves a combination of pegylated interferon-α and ribavirin. This therapy, which is commonly associated with sid...

    journal_title：Antiviral research

    pub_type： 杂志文章,评审

    doi：10.1016/j.antiviral.2012.07.006

    authors： Ip PP,Nijman HW,Wilschut J,Daemen T

    更新日期：2012-10-01 00:00:00

  • Identification of quinolone derivatives as effective anti-Dengue virus agents.

    abstract：:Dengue virus (DENV) infection is one of the most important infectious diseases in tropical and subtropical regions around the world. Previously, we performed an initial phenotypic screening of 7000 compounds using DENV type 2 (DENV2)-infected BHK-21 cells to identify small molecules which could inhibit virus replicati...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2020.104969

    authors： Nobori H,Uemura K,Toba S,Sanaki T,Shishido T,Hall WW,Orba Y,Sawa H,Sato A

    更新日期：2020-12-01 00:00:00

  • Inhibitory effect of Ephedrae herba, an oriental traditional medicine, on the growth of influenza A/PR/8 virus in MDCK cells.

    abstract：:Using several herbal extracts, we investigated whether certain Kampo medicines exert an inhibitory effect on the acidification of intracellular compartments such as endosomes and lysosomes (referred to as ELS), and thereby inhibit the growth of influenza A virus in Madin-Darby canine kidney cells. The vital fluorescen...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/s0166-3542(99)00067-4

    authors： Mantani N,Andoh T,Kawamata H,Terasawa K,Ochiai H

    更新日期：1999-12-31 00:00:00

  • Activity of compounds from Chinese herbal medicine Rhodiola kirilowii (Regel) Maxim against HCV NS3 serine protease.

    abstract：:Treatment of the chronic hepatitis C virus (HCV) infection is an unmet medical need, and the HCV NS3 serine protease (NS3-SP) has been used as an attractive target of antiviral screening against HCV. To find naturally chemical entities as lead compounds from which novel anti-HCV agents could be developed, bioassay-gui...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2007.06.001

    authors： Zuo G,Li Z,Chen L,Xu X

    更新日期：2007-10-01 00:00:00

  • Preferential selection of heterologous G3-VP7 gene in the genetic background of simian rotavirus SA11 detected by using a homotypic single-VP7 gene-substitution reassortant.

    abstract：:Introduction of segmented genomes into virion is an important process in viral replication of rotavirus. We previously studied the assortment of the VP7 gene segment (encoding outer capsid protein VP7) in the genetic background of simian rotavirus SA11 (G serotype 3, G3) and found the preferential selection of homolog...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/s0166-3542(98)00006-0

    authors： Okada J,Kobayashi N,Taniguchi K,Urasawa S

    更新日期：1998-04-01 00:00:00

  • The use of ampligen alone and in combination with ganciclovir and coumermycin A1 for the treatment of ducks congenitally-infected with duck hepatitis B virus.

    abstract：:Ampligen, a known immunomodulator and interferon inducer, was used alone and in combination with other antiviral agents to treat ducks congenitally-infected with duck hepatitis B virus. These antiviral agents included the conventional nucleoside analogue ganciclovir and the prokaryotic DNA gyrase B inhibitor coumermyc...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(93)90051-j

    authors： Niu J,Wang Y,Dixon R,Bowden S,Qiao M,Einck L,Locarnini S

    更新日期：1993-06-01 00:00:00

  • Genetic susceptibility in chronic viral hepatitis.

    abstract：:Infection with hepatitis B virus (HBV) or hepatitis C virus (HCV) may result in a number of different clinical outcomes. There is strong evidence in HBV infection that host genetic factors play a major role in determining the outcome of infection. A number of approaches may be used to determine the specific genetic fa...

    journal_title：Antiviral research

    pub_type： 杂志文章,评审

    doi：10.1016/s0166-3542(01)00175-9

    authors： Thursz M

    更新日期：2001-11-01 00:00:00

  • Are statins a viable option for the treatment of infections with the hepatitis C virus?

    abstract：:Statins are 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors that are widely used for the treatment of hypercholesterolemia. Besides their cholesterol-lowering effect, statins have been reported to have antiviral activity against a variety of viruses, including hepatitis C virus (HCV). Several stat...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2014.02.020

    authors： Verpaalen B,Neyts J,Delang L

    更新日期：2014-05-01 00:00:00

  • Semi-quantitative detection of viral RNA in influenza A virus-infected mice for evaluation of antiviral compounds.

    abstract：:The aim of the study was to establish a murine model for sensitive screening of potential compounds with in vitro anti-influenza A virus activity. The evaluation in this in vivo model is based on semi-quantitative detection of viral RNA using one-step reverse transcriptase polymerase chain reaction (RT-PCR). After int...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/s0166-3542(02)00184-5

    authors： Sauerbrei A,Ulbricht A,Wutzler P

    更新日期：2003-03-01 00:00:00

  • Treatment of chronic non-A non-B hepatitis with human interferon beta: a preliminary study.

    abstract：:Twenty-four patients with chronic non-A non-B hepatitis were randomly assigned to receive either human fibroblast interferon (HuIFN-beta) at doses of 1 or 3 million international units (MIU) per day for 4 or 12 weeks (12 patients) or to receive no therapy (12 patients), and were then compared with 5 patients with chro...

    journal_title：Antiviral research

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/0166-3542(89)90048-x

    authors： Kiyosawa K,Sodeyama T,Nakano Y,Yoda H,Tanaka E,Hayata T,Tsuchiya K,Yousuf M,Furuta S

    更新日期：1989-10-01 00:00:00

  • 6-Thioguanine inhibits rotavirus replication through suppression of Rac1 GDP/GTP cycling.

    abstract：:Rotavirus infection has emerged as an important cause of complications in organ transplantation recipients and might play a role in the pathogenesis of inflammatory bowel disease (IBD). 6-Thioguanine (6-TG) has been widely used as an immunosuppressive drug for organ recipients and treatment of IBD in the clinic. This ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2018.06.011

    authors： Yin Y,Chen S,Hakim MS,Wang W,Xu L,Dang W,Qu C,Verhaar AP,Su J,Fuhler GM,Peppelenbosch MP,Pan Q

    更新日期：2018-08-01 00:00:00

  • Anthraquinones as a new class of antiviral agents against human immunodeficiency virus.

    abstract：:Various anthraquinones substituted with hydroxyl, amino, halogen, carboxylic acid, substituted aromatic group, and sulfonate were tested to determine their activity against human immunodeficiency virus type 1 (HIV-1) in primary human lymphocytes. Among the compounds tested, polyphenolic and/or polysulfonate substitute...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(90)90071-e

    authors： Schinazi RF,Chu CK,Babu JR,Oswald BJ,Saalmann V,Cannon DL,Eriksson BF,Nasr M

    更新日期：1990-05-01 00:00:00

  • An engineered inhibitor RNA that efficiently interferes with hepatitis C virus translation and replication.

    abstract：:Hepatitis C virus (HCV) translation is mediated by a highly conserved internal ribosome entry site (IRES), mainly located at the 5'untranslatable region (5'UTR) of the viral genome. Viral protein synthesis clearly differs from that used by most cellular mRNAs, rendering the IRES an attractive target for novel antivira...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2012.02.015

    authors： Romero-López C,Berzal-Herranz B,Gómez J,Berzal-Herranz A

    更新日期：2012-05-01 00:00:00

  • Early therapy of feline leukemia virus infection (FeLV-FAIDS) with 9-(2-phosphonylmethoxyethyl)adenine (PMEA).

    abstract：:Cats infected with molecularly cloned FeLV-FAIDS develop an immunodeficiency syndrome characterized by persistent antigenemia, decline in circulating CD4+ T lymphocytes, and impaired T-cell-dependent immune responses and opportunistic infection. We evaluated the capacity of PMEA to inhibit the replication of FeLV-FAID...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(91)90060-5

    authors： Hoover EA,Ebner JP,Zeidner NS,Mullins JI

    更新日期：1991-07-01 00:00:00

  • Molecular basis of human immunodeficiency virus type 1 drug resistance: overview and recent developments.

    abstract：:The introduction of potent combination therapies in the mid-90s had a tremendous effect on AIDS mortality. However, drug resistance has been a major factor contributing to antiretroviral therapy failure. Currently, there are 26 drugs approved for treating human immunodeficiency virus (HIV) infections, although some of...

    journal_title：Antiviral research

    pub_type： 杂志文章,评审

    doi：10.1016/j.antiviral.2013.01.007

    authors： Menéndez-Arias L

    更新日期：2013-04-01 00:00:00

  • Modeling HCV cure after an ultra-short duration of therapy with direct acting agents.

    abstract：BACKGROUND:Cases of sustained-virological response (SVR or cure) after an ultra-short duration (≤27 days) of direct-acting antiviral (DAA)-based therapy, despite HCV being detected at end of treatment (EOT), have been reported. Established HCV mathematical models that predict the treatment duration required to achieve ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2017.06.019

    authors： Goyal A,Lurie Y,Meissner EG,Major M,Sansone N,Uprichard SL,Cotler SJ,Dahari H

    更新日期：2017-08-01 00:00:00

  • Effect of antiviral treatment in older patients hospitalized with confirmed influenza.

    abstract：:Seasonal influenza causes significant morbidity and mortality in people aged ≥65 years. Antiviral treatment can reduce complications and disease severity. The objective of this study was to investigate the effect of antiviral treatment in patients aged ≥65 years hospitalized with confirmed influenza in preventing inte...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2020.104785

    authors： Soldevila N,Toledo D,Ortiz de Lejarazu R,Tamames S,Castilla J,Astray J,Fernández MA,Martín V,Egurrola M,Morales Suárez-Varela M,Domínguez À,Working Group of Project PI12\/02079.

    更新日期：2020-06-01 00:00:00

  • Impact of single nucleotide polymorphisms in the essential HCV entry factor CD81 on HCV infectivity and neutralization.

    abstract：:End stage liver disease caused by chronic infection with the hepatitis C virus (HCV) is a leading indication for liver transplantation, yet outcomes are poor since the liver graft is rapidly re-infected by HCV. Antibodies against the essential HCV receptor CD81 have been shown to inhibit HCV cell entry in vitro and in...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2013.10.018

    authors： Deest M,Westhaus S,Steinmann E,Manns MP,von Hahn T,Ciesek S

    更新日期：2014-01-01 00:00:00

  • New pleconaril and [(biphenyloxy)propyl]isoxazole derivatives with substitutions in the central ring exhibit antiviral activity against pleconaril-resistant coxsackievirus B3.

    abstract：:Amino acid 1092 (AA1092) in capsid protein 1 of coxsackievirus B3 (CVB3) is located in close vicinity to the central phenoxy group of capsid binders (i.e. pleconaril). Whereas isoleucine is associated with drug susceptibility, leucine and methionine confer resistance to pleconaril. In the present study, novel analogue...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2008.09.002

    authors： Schmidtke M,Wutzler P,Zieger R,Riabova OB,Makarov VA

    更新日期：2009-01-01 00:00:00

  • Antiviral activity of phosphonoformate on rotavirus transcription and replication.

    abstract：:The antiviral effect of foscarnet (PFA) on the replication of rotavirus, a member of the Reoviridae, was studied. The pyrophosphate analogue is an effective inhibitor of several viral polymerases acting on the enzyme pyrophosphate binding site. Replication of rotavirus in MA104 cells using different u.o.i. was inhibit...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(94)00085-m

    authors： Ríos M,Muñoz M,Spencer E

    更新日期：1995-05-01 00:00:00

  • Activity of nucleic acid polymers in rodent models of HBV infection.

    abstract：:Nucleic acid polymers (NAPs) block the release of HBsAg from infected hepatocytes. These compounds have been previously shown to have the unique ability to eliminate serum surface antigen in DHBV-infected Pekin ducks and achieve multilog reduction of HBsAg or HBsAg loss in patients with chronic HBV infection and HBV/H...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2017.10.022

    authors： Schöneweis K,Motter N,Roppert PL,Lu M,Wang B,Roehl I,Glebe D,Yang D,Morrey JD,Roggendorf M,Vaillant A

    更新日期：2018-01-01 00:00:00