Abstract：
:RNA interference, mediated by short interfering RNAs (siRNAs), has been shown to have activity against a wide range of viruses and is a promising new antiviral therapy. Using multiple siRNAs that target conserved areas of the genome allows for increased chances of antiviral activity against different viral strains and also helps to prevent the emergence of escape mutants. In this study, four siRNAs were designed to target areas of conserved sequence between divergent strains of Venezuelan equine encephalitis virus (VEEV). A pool of these siRNAs inhibited the replication of all six strains of VEEV tested. A single nucleotide mismatch at the extreme 3' end of one of the siRNA sense strands did not affect antiviral activity but other mutations were not tolerated. Two strains of VEEV were tested for their abilities to overcome the inhibitory effects of RNA interference following 10 consecutive incubations in the presence of siRNAs. One strain remained susceptible throughout the course of the experiment but the other strain became resistant to the activity of siRNAs. Sequence analysis of the siRNA target sites in this strain showed that no mutations had been generated, indicating that the virus may had become resistant in some other manner. In the absence of effective antiviral drugs and vaccines to combat VEEV infection, these siRNAs offer a potential new therapeutic approach but, as with all antimicrobial agents, caution needs to be exercised with respect to the generation of resistance.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
O'Brien Ldoi
10.1016/j.antiviral.2006.11.007subject
Has Abstractpub_date
2007-07-01 00:00:00pages
20-9issue
1eissn
0166-3542issn
1872-9096pii
S0166-3542(06)00347-0journal_volume
75pub_type
杂志文章abstract：:While combination antiretroviral therapy (cART) has successfully converted HIV to a chronic but manageable infection in many parts of the world, HIV continues to persist within latent cellular reservoirs, which can become reactivated at any time to produce infectious virus. New therapies are therefore needed not only ...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2018.07.016
更新日期：2018-10-01 00:00:00
abstract：:There are two new drugs approved and several in development for treatment of chronic HCV; among them nitazoxanide (NTZ). Twelve HIV/HCV genotype 1 co-infected patients were enrolled prospectively to receive a 30 days course of oral NTZ 500 mg bid. This therapy was well tolerated in this group of HIV patients co-infect...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2011.10.006
更新日期：2011-12-01 00:00:00
abstract：:Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA), the stable genomic form as the template for viral transcription, plays a crucial role in viral persistence which remains a major global health problem. While accumulating evidence suggests the involvement of transcription factors and epigenetic machinery...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2018.03.008
更新日期：2018-05-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(93)90051-j
更新日期：1993-06-01 00:00:00
abstract：:Rabies is a lethal viral infection that can affect almost all mammals, including humans. To better understand the replication of Rabies lyssavirus, we investigated if the viral load in brains naturally infected with rabies influences viral internalization and viral growth kinetics in neuroblastoma cells, and if the vi...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2017.11.003
更新日期：2018-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0166-3542(95)00032-h
更新日期：1995-08-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2011.04.001
更新日期：2011-06-01 00:00:00
abstract：:Drug resistance testing has proven its use to guide treatment decisions in HIV-1 infected patients. Genotyping is the preferred technique for clinical drug resistance testing. Many factors complicate the interpretation of mutations towards therapy response, such that an interpretation system is necessary to help the c...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2006.05.003
更新日期：2006-09-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(93)90032-e
更新日期：1993-07-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2013.10.018
更新日期：2014-01-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(92)90048-a
更新日期：1992-02-01 00:00:00
abstract：:High-throughput genomic methods are now being used to study a wide variety of viral diseases, in an effort to understand how host responses to infection can lead either to efficient elimination of the pathogen or the development of severe disease. This article reviews how gene expression studies are addressing importa...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/j.antiviral.2008.12.005
更新日期：2009-03-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2015.01.008
更新日期：2015-04-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(00)00063-2
更新日期：2000-02-01 00:00:00
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journal_title：Antiviral research
pub_type： 临床试验,杂志文章,多中心研究
doi：10.1016/j.antiviral.2011.07.003
更新日期：2011-10-01 00:00:00
abstract：:Interleukin (IL)28-B polymorphism has been related to interferon response in the treatment of hepatitis C, but its role in chronic hepatitis B (CHB) therapy is still poorly understood. We aimed to investigate the effect of IL28-B polymorphisms in the treatment with pegylated-interferon (PEG-IFN) of patients with CHB. ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2013.11.014
更新日期：2014-02-01 00:00:00
abstract：:Lactoferrin (LF) is an iron binding glycoprotein that is present in several mucosal secretions. Many biological functions have been ascribed to LF. One of the functions of LF is the transport of metals, but LF is also an important component of the non-specific immune system, since LF has antimicrobial properties again...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/s0166-3542(01)00195-4
更新日期：2001-12-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2007.09.001
更新日期：2008-02-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/s0166-3542(95)00989-2
更新日期：1996-11-01 00:00:00
abstract：:We investigated the development of anti-pre-S(2) antibodies by enzyme immunoassay and by Western blot analysis in a vaccination study in haemodialysis patients; both the Pasteur plasma vaccine retaining the pre-S(2) epitopes and the Merck, Sharp and Dohme (MSD) plasma vaccine containing only HBsAg were used. By enzyme...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/0166-3542(89)90071-5
更新日期：1989-09-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2015.05.006
更新日期：2015-08-01 00:00:00
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journal_title：Antiviral research
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0166-3542(03)00065-2
更新日期：2003-06-01 00:00:00
abstract：:Zika virus (ZIKV) is a mosquito-borne flavivirus associated with severe neonatal birth defects, but the causative mechanism is incompletely understood. ZIKV shares sequence homology and early clinical manifestations with yellow fever virus (YFV) and dengue virus (DENV) and are all transmitted in urban cycles by the sa...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2018.01.003
更新日期：2018-03-01 00:00:00
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journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2010.10.009
更新日期：2011-01-01 00:00:00
abstract：:Innate, non-specific, resistance mechanisms are important barriers to pathogens, particularly delaying virus multiplication at the onset of infections. These innate defense mechanisms include a series of mechanical barriers, pre-existing inhibitory molecules, and cellular responses with antimicrobial activity. The ant...
journal_title：Antiviral research
pub_type： 杂志文章,评审
doi：10.1016/s0166-3542(00)00126-1
更新日期：2000-11-01 00:00:00
abstract：:The global analysis of neuraminidase inhibitor (NAI) susceptibility of influenza viruses has been conducted since the 2012-13 period. In 2018 a novel cap-dependent endonuclease inhibitor, baloxavir, that targets polymerase acidic subunit (PA) was approved for the treatment of influenza virus infection in Japan and the...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104718
更新日期：2020-03-01 00:00:00
abstract：:HIV-infected patients are at increased risk of decreased bone mineral density. Several studies have implicated antiretroviral therapy as a contributor to the decreased bone mineral density seen in treated HIV-1 patients. Whilst the exact molecular mechanisms underlying decreased bone density remain to be elucidated, i...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.12.003
更新日期：2007-04-01 00:00:00
abstract：:There is a pressing need for new vaccines against alphaviruses, which can cause fatal encephalitis (Venezuelan equine encephalitis virus (VEEV) and others) and severe arthralgia (e.g. Chikungunya virus, CHIKV). These positive-strand RNA viruses are diverse and evolve rapidly, meaning that the sequence of any vaccine s...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2020.104905
更新日期：2020-10-01 00:00:00
abstract：:Favipiravir (T-705) is a new anti-influenza drug approved for human use in Japan and progressing through Phase 3 clinical trials in the U.S. In addition to its potent inhibitory effects against influenza virus infection, the compound has been shown to be broadly active against RNA viruses from 9 different families, in...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2015.07.003
更新日期：2015-09-01 00:00:00
abstract：:SeqHepB is a combination of a HBV genome sequence analysis program and a relational database that houses data collected from multiple data sources. Registered users can access the sequence analysis component of SeqHepB online for rapid and detailed interrogation of HBV genomic sequences. Its main function is to determ...
journal_title：Antiviral research
pub_type： 杂志文章
doi：10.1016/j.antiviral.2006.11.014
更新日期：2007-07-01 00:00:00
