Abstract:
:RNA interference, mediated by short interfering RNAs (siRNAs), has been shown to have activity against a wide range of viruses and is a promising new antiviral therapy. Using multiple siRNAs that target conserved areas of the genome allows for increased chances of antiviral activity against different viral strains and also helps to prevent the emergence of escape mutants. In this study, four siRNAs were designed to target areas of conserved sequence between divergent strains of Venezuelan equine encephalitis virus (VEEV). A pool of these siRNAs inhibited the replication of all six strains of VEEV tested. A single nucleotide mismatch at the extreme 3' end of one of the siRNA sense strands did not affect antiviral activity but other mutations were not tolerated. Two strains of VEEV were tested for their abilities to overcome the inhibitory effects of RNA interference following 10 consecutive incubations in the presence of siRNAs. One strain remained susceptible throughout the course of the experiment but the other strain became resistant to the activity of siRNAs. Sequence analysis of the siRNA target sites in this strain showed that no mutations had been generated, indicating that the virus may had become resistant in some other manner. In the absence of effective antiviral drugs and vaccines to combat VEEV infection, these siRNAs offer a potential new therapeutic approach but, as with all antimicrobial agents, caution needs to be exercised with respect to the generation of resistance.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
O'Brien Ldoi
10.1016/j.antiviral.2006.11.007subject
Has Abstractpub_date
2007-07-01 00:00:00pages
20-9issue
1eissn
0166-3542issn
1872-9096pii
S0166-3542(06)00347-0journal_volume
75pub_type
杂志文章abstract::We have investigated the inhibitory effect of trans-cinnamaldehyde (CA), one of the principal constituents of essential oil derived from Cinnamomi cortex, on the growth of influenza A/PR/8 virus in vitro and in vivo. When 1-h drug treatment was initiated at various times post-infection (p.i.) in Madin-Darby canine kid...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2007.01.003
更新日期:2007-04-01 00:00:00
abstract::Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded R...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2020.104874
更新日期:2020-10-01 00:00:00
abstract::JNJ63623872 (formerly known as VX-787) is an inhibitor of influenza A virus polymerases through interaction with the viral PB2 subunit. This interaction blocks the cap-snatching activity of the virus that is essential for virus replication. Previously published work has documented antiviral activity of JNJ63623872 in ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.10.009
更新日期:2016-12-01 00:00:00
abstract::Efavirenz (EFV), a first generation non-nucleoside analogue reverse transcriptase inhibitor widely employed in combination antiretroviral therapy regimens over the last 20 years, has been associated with a wide range of neuropsychiatric effects and has also been linked with HIV-associated neurocognitive disorder (HAND...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2019.04.015
更新日期:2019-08-01 00:00:00
abstract::Human CMV causes a number of diseases that cause considerable morbidity and that can be life-threatening in immunocompromised patients, particularly those with AIDS. Ganciclovir (GCV) and Foscarnet (PFA) are currently the drugs of choice for management of CMV disease. Both are not without side effects and have a relat...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(94)90048-5
更新日期:1994-05-01 00:00:00
abstract::The single tablet regimen of the nucleoside/nucleotide reverse transcriptase inhibitors (NRTIs) tenofovir disoproxil fumarate (TDF), emtricitabine (FTC), and the non-nucleoside reverse transcriptase inhibitor (NNRTI) rilpivirine (RPV) is approved for the treatment of HIV-1 infection in treatment-naïve adults. Previous...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2013.11.010
更新日期:2014-01-01 00:00:00
abstract::The influenza A (H1N1) virus is a highly contagious acute respiratory disease affecting pigs and humans. This disease causes severe economic loss in many countries, and developing mucosal vaccines is an efficient strategy to control the influenza virus. The neonatal Fc receptor (FcRn) plays an important role in transf...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.11.025
更新日期:2017-02-01 00:00:00
abstract::A new adamantane derivative N,N'-bis (ethylene)P(1-adamantyl)-phosphonis diamide, was found to inhibit Rous sarcoma virus replication in much the same manner as reported for the parent compound, 1-adamantanamine hydrochloride. ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(81)90017-6
更新日期:1981-11-01 00:00:00
abstract::The model of simian immunodeficiency virus (SIV) infection in rhesus macaques was used to evaluate the effects of recombinant human interferon alpha, Hu IFN-alpha 2b and Hu IFN-gamma B,D, at two doses. Administration began 1 day prior to infection and was continued for 90 days postinfection. Both interferons suppresse...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(95)00955-8
更新日期:1996-08-01 00:00:00
abstract::Derivatives of beta-lactam antibiotics of the cephalosporin type at 0.02-1 mM concentrations interfered with in vitro replication of two DNA-containing viruses, herpes simplex I and vaccinia, but showed no effects on two RNA-viruses, lymphocytic choriomeningitis virus and vesicular stomatitis virus, or on cell viabili...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(88)90014-9
更新日期:1988-11-01 00:00:00
abstract:BACKGROUND:Cyclophilin inhibitors have shown activity against a variety of viruses, including HCV. NIM811, a novel, non-immunosuppressive cyclophilin inhibitor was studied in ascending doses in a randomized, double-blind, placebo-controlled 14-day trial in genotype 1 HCV patients. Doses of 10 up to 600 mg were given or...
journal_title:Antiviral research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.antiviral.2011.01.003
更新日期:2011-03-01 00:00:00
abstract::Infection by one of the 4 distinct serotypes of dengue virus (DENV) threatens >40% of the world's population, with no efficacious vaccine or antiviral agent currently available. DENV replication through the virus-encoded nonstructural protein (NS) 5 protein occurs in the infected cell cytoplasm, but NS5 from DENV2 has...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2013.06.002
更新日期:2013-09-01 00:00:00
abstract::The viral genotype and serum viral level influence the response to interferon (IFN) treatment in patients with chronic hepatitis C virus (HCV) viremia. The aim of this study was to investigate a possible relationship between early virological response and helper T (Th) cell cytokine expansion by 4 weeks of ribavirin (...
journal_title:Antiviral research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.antiviral.2005.04.001
更新日期:2005-07-01 00:00:00
abstract::The emergence of the Middle East respiratory syndrome coronavirus (MERS-CoV) in 2012 marked the second time that a new, highly pathogenic coronavirus has emerged in the human population in the 21st century. In this review, we discuss the current state of knowledge of animal models of MERS-CoV infection. Commonly used ...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2015.07.005
更新日期:2015-10-01 00:00:00
abstract::Human cytomegalovirus terminase complex cleaves the concatemeric genomic viral DNA into unit lengths during genome packaging and particle assembly. Terminase complex ATPase and endonuclease activity is provided by the viral protein pUL89. pUL89 is an attractive drug target because its activities are required for infec...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.01.015
更新日期:2018-04-01 00:00:00
abstract::We have investigated viral breakthrough during a long-term culture of HIV-1-infected cells with the non-nucleoside reverse transcriptase inhibitors (NNRTIs) 6-benzyl-1-ethoxymethyl-5-isopropyluracil (MKC-442), nevirapine and loviride (alpha-APA). When the compounds were examined for their inhibitory effects on HIV-1 (...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(96)00946-1
更新日期:1996-06-01 00:00:00
abstract::Previous studies have demonstrated that cyclopentenone prostaglandins (cyPGs) inhibit the replication of a wide variety of DNA and RNA viruses in different mammalian cell types. We investigated a new role for prostaglandin A1 (PGA1) in the inhibition of hepatitis C virus (HCV)-IRES-mediated translation. PGA1 exhibited...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2015.01.013
更新日期:2015-05-01 00:00:00
abstract::Drug resistance resulting from reverse transcriptase (RT) mutations is one of the main obstacles to successful hepatitis B virus (HBV) therapy. Indeed, HBV treatment guidelines recommend HBV genotypic resistance testing for patients receiving nucleos(t)ide RT inhibitors (N(t)RTIs) who develop virological failure. N(t)...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2010.09.012
更新日期:2010-12-01 00:00:00
abstract::Pyrosequencing has been successfully used to monitor resistance in influenza A viruses to the first class of anti-influenza drugs, M2 blockers (adamantanes). In contrast to M2 blockers, resistance to neuraminidase (NA) inhibitors (NAIs) is subtype- and drug-specific. Here, we designed a pyrosequencing assay for detect...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2008.08.008
更新日期:2009-01-01 00:00:00
abstract::Emergence of drug-resistant human cytomegalovirus (HCMV) strains is a substantial problem during treatment of HCMV infections in immunocompromised patients. The Z-isomers of 2-hydroxymethylcyclopropylidenemethyl adenine (synadenol) and guanine (synguanol) were previously shown to be potent inhibitors of AD169 and Town...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(02)00129-8
更新日期:2002-12-01 00:00:00
abstract::Although in vitro studies have been performed to dissect the mechanism of action of IFNα, detailed in vivo studies on the long-term effects of IFNα on monocytes have not been performed. Here we examined peripheral blood from 14 chronic HCV patients at baseline and 12 weeks after start of IFNα-based therapy. Monocytes ...
journal_title:Antiviral research
pub_type: 临床试验,杂志文章
doi:10.1016/j.antiviral.2014.06.020
更新日期:2014-09-01 00:00:00
abstract::We have previously reported a new class of dendrimers with tryptophan (Trp) residues on the surface that show dual antiviral activities against HIV and enterovirus EV71. The prototype compound of this family is a derivative of pentaerythritol with 12 peripheral Trp groups and trivalent spacer arms. Here a novel series...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2019.06.006
更新日期:2019-08-01 00:00:00
abstract::During studies of the antiviral activity of chalcone Ro-09-0410 on human rhinovirus type 9 (RV9) chalcone-resistant strains of RV9 were isolated and appeared with a frequency of about 10(-5) in chalcone sensitive stock. Chalcone-dependent viruses were found after further passage. Some characteristics of the resistant ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(87)90085-4
更新日期:1987-08-01 00:00:00
abstract::The present study was undertaken to examine the potential of rectal route of immunization for induction of protective immunity in the female genital tract against genital herpes infection in mice. A single rectal immunization of female C57Bl/6 mice with live attenuated herpes simplex virus type 2 lacking thymidine kin...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2007.12.014
更新日期:2008-06-01 00:00:00
abstract::Using an in vitro system we measured the corneal epithelial cytotoxicity and the antiviral activity of the antiviral agents idoxuridine (IDU), trifluridine (TFT), ethyldeoxyuridine (EDU), and (E)-5-(2-Bromovinyl)-2'-deoxyuridine (BVDU). Confluent rabbit corneal epithelial cell cultures were established, and the antivi...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(88)90057-5
更新日期:1988-07-01 00:00:00
abstract::We report the design, synthesis, and biological evaluation of a class of 1H-pyrido[2,1-b][1,3]benzothiazol-1-ones originated from compound 1, previously identified as anti-flavivirus agent. Some of the new compounds showed activity in low μM range with reasonable selectivity against Dengue 2, Yellow fever (Bolivia str...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2019.03.004
更新日期:2019-07-01 00:00:00
abstract::The replication cycle of the human cytomegalovirus (HCMV) is characterized by the expression of immediate early (IE), early (E), and late (L) gene regions. Current antiviral strategies are directed against the viral DNA polymerase expressed during the early phase of infection. The regulation of the IE-1 and IE-2 gene ...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/s0166-3542(01)00126-7
更新日期:2001-03-01 00:00:00
abstract::Antigen presenting liposomes (APLs) containing both liposome encapsulated (44%) and free (56%) recombinant glycoprotein D of herpes simplex virus type-2 (rgD-2) were characterized with respect to the interaction of the antigen with the lipid bilayer and the biological activities provided by each form of rgD-2. We foun...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(90)90037-8
更新日期:1990-04-01 00:00:00
abstract::Inosine monophosphate dehydrogenase (IMPDH) catalyzes an essential step in the de novo biosynthesis of guanine nucleotide, namely, the conversion of IMP to XMP. The depletion of the intracellular GTP and dGTP pools is the major event occurring in the cells exposed to the inhibitors such as mycophenolic acid. The prese...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2010.10.009
更新日期:2011-01-01 00:00:00
abstract::Preventive intraperitoneal trehalose dimycolate (TDM) treatment of mice, inoculated with encephalomyocarditis (EMC) virus by the same route, caused restriction of virus growth in the peritoneum, which was correlated to IFN production in peritoneal fluids prior to infection. Peritoneal macrophages from TDM-treated mice...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(95)00047-p
更新日期:1995-10-01 00:00:00