当前位置： SCI文献检索 > ANTIVIRAL RESEARCH期刊下所有文献 > Analysis of the transcriptome and immune function of monocytes during IFNα-based therapy in chronic HCV revealed induction of TLR7 responsiveness.

Analysis of the transcriptome and immune function of monocytes during IFNα-based therapy in chronic HCV revealed induction of TLR7 responsiveness.

Abstract：

:Although in vitro studies have been performed to dissect the mechanism of action of IFNα, detailed in vivo studies on the long-term effects of IFNα on monocytes have not been performed. Here we examined peripheral blood from 14 chronic HCV patients at baseline and 12 weeks after start of IFNα-based therapy. Monocytes were phenotyped by flow-cytometry and their function evaluated upon TLR stimulation and assessed by multiplex cytokine assays. During therapy of HCV patients, monocytes displayed a hyperactive state as evidenced by increased TLR-induced pro-inflammatory cytokine levels, as well as enhanced CD69 and CD83 mRNA and protein expression. Moreover, monocytes from 8 patients at baseline and 12 weeks after start of IFNα-based therapy were transcriptomically profiled by high throughput RNA-sequencing. Detailed RNA-seq analysis of monocytes showed significant ISG mRNA induction during therapy. Importantly, IFNα-based therapy activated TLR7 signaling pathways, as demonstrated by up-regulated expression of TLR7, MyD88, and IRF7 mRNA, whereas other TLR family members as well as CD1c, CLEC4C, and CLEC9A were not induced. The induction of TLR7 responsiveness of monocytes by IFNα in vivo in HCV patients is relevant for the development of TLR7 agonists that are currently under development as a promising immunotherapeutic compounds to treat chronic viral hepatitis.

journal_name

Antiviral Res

journal_title

Antiviral research

authors

Hou J,Groothuismink ZM,Koning L,Roomer R,van IJcken WF,Kreefft K,Liu BS,Janssen HL,de Knegt RJ,Boonstra A

doi

10.1016/j.antiviral.2014.06.020

subject

Has Abstract

pub_date

2014-09-01 00:00:00

pages

116-24

eissn

0166-3542

issn

1872-9096

pii

S0166-3542(14)00182-X

journal_volume

109

pub_type

临床试验,杂志文章

相关文献

ANTIVIRAL RESEARCH文献大全
  • Drastic decrease of transcription activity due to hypermutated long terminal repeat (LTR) region in different HIV-1 subtypes and recombinants.

    abstract：:Transcriptional activation of HIV-1 gene expression is partially controlled by the interaction between viral and cellular transcription factors acting at HIV-1 long terminal repeat (LTR) sequences. HIV-1 subtyping at LTR region and nucleotide LTR variability from clinical samples in 48 subjects carrying different HIV-...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2010.08.007

    authors： de Arellano ER,Alcamí J,López M,Soriano V,Holguín A

    更新日期：2010-11-01 00:00:00

  • Susceptibilities of enterovirus D68, enterovirus 71, and rhinovirus 87 strains to various antiviral compounds.

    abstract：:Compounds were evaluated for antiviral activity in rhabdomyosarcoma (RD) cells against a recent 2014 clinical isolate of enterovirus D68 (EV-D68), a 1962 strain of EV-68D, rhinovirus 87 (RV-87, serologically the same as EV-D68), and enterovirus 71 (EV-71). Test substances included known-active antipicornavirus agents ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2016.04.003

    authors： Smee DF,Evans WJ,Nicolaou KC,Tarbet EB,Day CW

    更新日期：2016-07-01 00:00:00

  • Dose proportional inhibition of HIV-1 replication by mycophenolic acid and synergistic inhibition in combination with abacavir, didanosine, and tenofovir.

    abstract：:Mycophenolate mofetil (MMF), a therapeutically used inhibitor of inosine monophosphate dehydrogenase is hydrolyzed to its active metabolite mycophenolic acid (MPA) in vivo. MPA exhibits anti-HIV activity in vitro. We tested MPA alone and in combination with abacavir (ABC), didanosine (DDI), lamivudine (3TC) and tenofo...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/s0166-3542(02)00006-2

    authors： Hossain MM,Coull JJ,Drusano GL,Margolis DM

    更新日期：2002-07-01 00:00:00

  • Antiviral activity of Viracea against acyclovir susceptible and acyclovir resistant strains of herpes simplex virus.

    abstract：:Viracea, a topical microbicide, is a blend of benzalkonium chloride and phytochemicals derived from Echinacea purpurea and is a proprietary formula from Destiny BioMediX Corp. Viracea was tested against 40 strains of herpes simplex virus (HSV): 15 strains (five HSV-1 and ten HSV-2) were resistant to acyclovir (ACV-R) ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/s0166-3542(98)00027-8

    authors： Thompson KD

    更新日期：1998-07-01 00:00:00

  • Rapamycin enhances aplaviroc anti-HIV activity: implications for the clinical development of novel CCR5 antagonists.

    abstract：:Maraviroc, the only CCR5 antagonist HIV inhibitor currently approved, has potent antiviral activity in treatment-experienced individuals infected with CCR5-using HIV-1 (R5 HIV-1). However, recent data from the MOTIVATE trials indicate that R5 HIV-1 can develop resistance to Maraviroc, underscoring the need for additio...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2009.02.199

    authors： Latinovic O,Heredia A,Gallo RC,Reitz M,Le N,Redfield RR

    更新日期：2009-07-01 00:00:00

  • Everolimus delayed and suppressed cytomegalovirus DNA synthesis, spread of the infection, and alleviated cytomegalovirus infection.

    abstract：:Everolimus is an inhibitor of mammalian target of rapamycin (mTOR) and reduces the risk of cytomegalovirus (CMV) infection in transplant recipients. Everolimus inhibits mTOR complex 1, which regulates factors involved in several crucial cellular functions and is required for CMV replication. However, it is not clear h...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2018.12.004

    authors： Tan L,Sato N,Shiraki A,Yanagita M,Yoshida Y,Takemura Y,Shiraki K

    更新日期：2019-02-01 00:00:00

  • Inhibitory effect of Distamycin-A and a pyrazino-pyrazine derivative on tomato spotted wilt virus.

    abstract：:Distamycin-A hydrochloride, a synthetic antibiotic, and 2,3-dihydroxy-6-bromo-pyrazino (2,3-beta) pyrazine derivative, were used against tomato spotted wilt virus (TSWV) in tobacco plants. The drugs were applied to the leaves at concentrations of 200 and 400 mg/l. The results showed that both drugs delayed virus sprea...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(83)90032-3

    authors： De Fazio G,Kudamatsu M

    更新日期：1983-08-01 00:00:00

  • Effect of tiazofurin on tomato plants infected with tomato spotted wilt virus.

    abstract：:Tiazofurin (2-beta-D-ribofuranosylthiazole-4-carboxamide) was examined for its activity against tomato-spotted wilt virus (TSWV) in tomato plants. Solutions containing 50, 100, 200, 400 and 800 mg/l of the drug were sprayed onto the leaves. The results showed that 100 and 200 mg/l were the most efficient concentration...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(84)90003-2

    authors： Caner J,Amélia M,Alexandre V,Vicente M

    更新日期：1984-12-01 00:00:00

  • Effects of apricitabine and other nucleoside reverse transcriptase inhibitors on replication of mitochondrial DNA in HepG2 cells.

    abstract：:Several nucleoside reverse transcriptase inhibitors are associated with mitochondrial toxicity resulting from inhibition of DNA polymerase-gamma. This study compared the effects on mitochondrial DNA of apricitabine (previously referred to as AVX754 or SPD754), a novel cytidine analogue under development for the treatm...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2007.05.004

    authors： de Baar MP,de Rooij ER,Smolders KG,van Schijndel HB,Timmermans EC,Bethell R

    更新日期：2007-10-01 00:00:00

  • Comparison of human cytomegalovirus DNA polymerase activity for ganciclovir-resistant and -sensitive clinical strains.

    abstract：:Previously, we found that a ganciclovir (GCV)-resistant clinical human cytomegalovirus (HCMV) isolate had an amino acid substitution at codon 501 (Leu --> Phe) in the delta-region C of the DNA polymerase gene. DNA polymerases have now been (partially) purified from both the GCV-resistant and sensitive parental strains...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/s0166-3542(00)00063-2

    authors： Kariya M,Mori S,Eizuru Y

    更新日期：2000-02-01 00:00:00

  • Efficacy of bromovinyldeoxyuridine in the treatment of herpes simplex virus and varicella-zoster virus eye infections.

    abstract：:As has been established in rabbits, (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) is superior to 5-iodo-2'-deoxyuridine (IDU) in the topical treatment of epithelial HSV-1 (herpes simplex virus type 1) keratitis, and superior to 5-trifluoromethyl-2'-deoxyuridine (TFT) in the topical treatment of deep stromal HSV-1 kerati...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(84)90033-0

    authors： Maudgal PC,De Clercq E,Missotten L

    更新日期：1984-10-01 00:00:00

  • Role of IL28-B polymorphisms in the treatment of chronic hepatitis B HBeAg-negative patients with peginterferon.

    abstract：:Interleukin (IL)28-B polymorphism has been related to interferon response in the treatment of hepatitis C, but its role in chronic hepatitis B (CHB) therapy is still poorly understood. We aimed to investigate the effect of IL28-B polymorphisms in the treatment with pegylated-interferon (PEG-IFN) of patients with CHB. ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2013.11.014

    authors： Boglione L,Cusato J,Allegra S,Esposito I,Patti F,Cariti G,Di Perri G,D'Avolio A

    更新日期：2014-02-01 00:00:00

  • Genetic analysis of a ganciclovir-resistant human cytomegalovirus mutant.

    abstract：:We isolated a ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) from a laboratory strain, AD169, and analysed the mutant. Attempts were also made to identify directly the mutated gene. The 50% inhibitory concentration (IC50) of GCV for the mutant strain was five times higher than that of the wild-type strain. T...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/s0166-3542(98)00051-5

    authors： Faizi Khan R,Mori S,Eizuru Y,Kumura Ishii K,Minamishima Y

    更新日期：1998-12-01 00:00:00

  • Benzothiazepinecarboxamides: Novel hepatitis C virus inhibitors that interfere with viral entry and the generation of infectious virions.

    abstract：:Upon screening synthetic small molecule libraries with the infectious hepatitis C virus (HCV) cell culture system, we identified a benzothiazepinecarboxamide (BTC) scaffold that inhibits HCV. A structure-activity relationship (SAR) study with BTCs was performed, and modifications that led to nanomolar antiviral activi...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2016.01.010

    authors： Kim HY,Kong S,Oh S,Yang J,Jo E,Ko Y,Kim SH,Hwang JY,Song R,Windisch MP

    更新日期：2016-05-01 00:00:00

  • Interferon plus Chinese herbs are associated with higher sustained virological response than interferon alone in chronic Hepatitis C: a meta-analysis of randomised trials.

    abstract：BACKGROUND/AIMS:Traditional Chinese herbal therapies are widely used for the treatment of chronic hepatitis C (CHC) in Asia. The aim of this study was to perform a meta-analysis of randomised controlled trials (RCTs) comparing interferon therapies with Chinese herbal therapies and/or interferon plus Chinese herb therap...

    journal_title：Antiviral research

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.antiviral.2010.12.003

    authors： Zhao S,Liu E,Wei K,Lu S,Chu Y,Li Y,Wang Y,Huang B,Chen Y,Yang P

    更新日期：2011-02-01 00:00:00

  • Efficacy of (E)-5-(2-bromovinyl)-2'-deoxyuridine in the treatment of experimental herpes simplex virus encephalitis in mice.

    abstract：:Systemic treatment of mice with (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) showed a significant therapeutic efficacy against herpes simplex type 1 virus (HSV-1) encephalitis. With treatment initiated 12 h after viral inoculation and continued for 10 consecutive days, BVDU administered intraperitoneally in daily doses...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(83)90010-4

    authors： Park NH,Pavan-Langston D,de Clercq E

    更新日期：1983-03-01 00:00:00

  • The L1 protein of human papilloma virus 16 expressed by a fowlpox virus recombinant can assemble into virus-like particles in mammalian cell lines but elicits a non-neutralising humoral response.

    abstract：:Human papilloma virus (HPV)-16 is the prevalent genotype associated with cervical tumours. Virus-like-particle (VLP)-based vaccines have proven to be effective in limiting new infections of high-risk HPVs, but their high cost has hampered their use, especially in the poor developing countries. Avipox-based recombinant...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2015.01.012

    authors： Bissa M,Zanotto C,Pacchioni S,Volonté L,Venuti A,Lembo D,De Giuli Morghen C,Radaelli A

    更新日期：2015-04-01 00:00:00

  • Dead-end complexes contribute to the synergistic inhibition of HIV-1 RT by the combination of rilpivirine, emtricitabine, and tenofovir.

    abstract：:The single tablet regimen of the nucleoside/nucleotide reverse transcriptase inhibitors (NRTIs) tenofovir disoproxil fumarate (TDF), emtricitabine (FTC), and the non-nucleoside reverse transcriptase inhibitor (NNRTI) rilpivirine (RPV) is approved for the treatment of HIV-1 infection in treatment-naïve adults. Previous...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2013.11.010

    authors： Kulkarni R,Feng JY,Miller MD,White KL

    更新日期：2014-01-01 00:00:00

  • Studies of rhinovirus resistant to an antiviral chalcone.

    abstract：:During studies of the antiviral activity of chalcone Ro-09-0410 on human rhinovirus type 9 (RV9) chalcone-resistant strains of RV9 were isolated and appeared with a frequency of about 10(-5) in chalcone sensitive stock. Chalcone-dependent viruses were found after further passage. Some characteristics of the resistant ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(87)90085-4

    authors： Ahmad AL,Dowsett AB,Tyrrell DA

    更新日期：1987-08-01 00:00:00

  • Producing physicochemical property consensus alphavirus protein antigens for broad spectrum vaccine design.

    abstract：:There is a pressing need for new vaccines against alphaviruses, which can cause fatal encephalitis (Venezuelan equine encephalitis virus (VEEV) and others) and severe arthralgia (e.g. Chikungunya virus, CHIKV). These positive-strand RNA viruses are diverse and evolve rapidly, meaning that the sequence of any vaccine s...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2020.104905

    authors： Baker WS,Negi S,Braun W,Schein CH

    更新日期：2020-10-01 00:00:00

  • Compounds that target host cell proteins prevent varicella-zoster virus replication in culture, ex vivo, and in SCID-Hu mice.

    abstract：:Varicella-zoster virus (VZV) replicates in quiescent T cells, neurons, and skin cells. In cultured fibroblasts (HFFs), VZV induces host cyclin expression and cyclin-dependent kinase (CDK) activity without causing cell cycle progression. CDK1/cyclin B1 phosphorylates the major viral transactivator, and the CDK inhibito...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2010.03.007

    authors： Rowe J,Greenblatt RJ,Liu D,Moffat JF

    更新日期：2010-06-01 00:00:00

  • Inhibition of reverse transcriptase activity of avian myeloblastosis virus by pyrophosphate analogues.

    abstract：:Several pyrophosphate analogues have been studied for their effects on avian myeloblastosis virus reverse transcriptase and on cellular DNA polymerase alpha. Examination of structure-activity relationships for these compounds revealed that two acidic groups connected by a short bridge were necessary, but not sufficien...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(82)90028-6

    authors： Eriksson B,Stening G,Oberg B

    更新日期：1982-05-01 00:00:00

  • Intravascular distribution of zidovudine: role of plasma proteins and whole blood components.

    abstract：:Knowledge of drug protein-binding and blood cell partitioning may be important for evaluating the pharmacokinetic parameters of zidovudine, particularly because of its intracellular site of action and potential to induce side effects. Equilibrium dialysis studies of zidovudine were performed over 2 h to identify the e...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(93)90032-e

    authors： Luzier A,Morse GD

    更新日期：1993-07-01 00:00:00

  • Suramin inhibits EV71 infection.

    abstract：:Enterovirus-71 (EV71) is one of the major causative reagents for hand-foot-and-mouth disease. In particular, EV71 causes severe central nervous system infections and leads to numerous dead cases. Although several inactivated whole-virus vaccines have entered in clinical trials, no antiviral agent has been provided for...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2013.12.008

    authors： Wang Y,Qing J,Sun Y,Rao Z

    更新日期：2014-03-01 00:00:00

  • Rift Valley fever virus: Unanswered questions.

    abstract：:This mosquito-borne pathogen of humans and animals respects no international or geographic boundaries. It is currently found in parts of Africa and the Arabian Peninsula where periodic outbreaks of severe and fatal disease occur, and threatens to spread into other geographic regions. In recent years, modern molecular ...

    journal_title：Antiviral research

    pub_type： 杂志文章,评审

    doi：10.1016/j.antiviral.2016.07.005

    authors： Bird BH,McElroy AK

    更新日期：2016-08-01 00:00:00

  • Impact of single nucleotide polymorphisms in the essential HCV entry factor CD81 on HCV infectivity and neutralization.

    abstract：:End stage liver disease caused by chronic infection with the hepatitis C virus (HCV) is a leading indication for liver transplantation, yet outcomes are poor since the liver graft is rapidly re-infected by HCV. Antibodies against the essential HCV receptor CD81 have been shown to inhibit HCV cell entry in vitro and in...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2013.10.018

    authors： Deest M,Westhaus S,Steinmann E,Manns MP,von Hahn T,Ciesek S

    更新日期：2014-01-01 00:00:00

  • Mode of action of 2-furylmercury chloride, an anti-rhinovirus compound.

    abstract：:2-Furylmercury chloride (2-FMC), an organic mercury derivative, has been found to inhibit the replication of all tested human rhinovirus (HRV) serotypes belonging to the antiviral group B and a limited number of HRV serotypes belonging to the antiviral group A. The mechanism of action of 2-FMC was tested against HRV-2...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2003.10.004

    authors： Verheyden B,Andries K,Rombaut B

    更新日期：2004-03-01 00:00:00

  • Emergence of human immunodeficiency virus type 1 variants containing the Q151M complex in children receiving long-term antiretroviral chemotherapy.

    abstract：:We examined 28 children with HIV-1 infection who were not responding to existing antiviral regimens and were enrolled into clinical trials conducted at the National Cancer Institute to receive salvage therapy. In 3 of the 28 patients (10.7%), the Q151M complex amino acid substitutions were identified. The three patien...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2007.02.004

    authors： Harada S,Hazra R,Tamiya S,Zeichner SL,Mitsuya H

    更新日期：2007-08-01 00:00:00

  • Inhibition of human parainfluenza virus type 3 infection by novel small molecules.

    abstract：:Human parainfluenza virus type 3 (HPIV3) is an important respiratory tract pathogen of infants and children. There are no vaccines or antivirals currently approved for prevention or treatment of HPIV3 infection. Towards developing an antiviral therapy to combat HPIV3 infection, we have established a green fluorescent ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2007.09.001

    authors： Mao H,Thakur CS,Chattopadhyay S,Silverman RH,Gudkov A,Banerjee AK

    更新日期：2008-02-01 00:00:00

  • Patterns of hepatocellular carcinoma development in hepatitis B virus and hepatitis C virus related cirrhosis.

    abstract：:To compare incidence, risk factors and morphologic pattern of hepatocellular carcinoma (HCC) development in hepatitis B virus (HBV) and hepatitis C virus (HCV) related cirrhosis, 401 patients were followed prospectively by periodic ultrasound examination for 14-189 months (mean: 84.8+/-36.7). During follow-up, 77 (19....

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/s0166-3542(01)00185-1

    authors： Benvegnù L,Alberti A

    更新日期：2001-11-01 00:00:00