当前位置： SCI文献检索 > ANTIVIRAL RESEARCH期刊下所有文献 > Short peptide nucleic acids (PNA) inhibit hepatitis C virus internal ribosome entry site (IRES) dependent translation in vitro.

Short peptide nucleic acids (PNA) inhibit hepatitis C virus internal ribosome entry site (IRES) dependent translation in vitro.

Abstract：

:The internal ribosome entry site (IRES) of hepatitis C virus (HCV) which governs the initiation of protein synthesis from viral RNA represents an ideal target for antisense approaches. Using an original bicistronic plasmid, we first established that sequence and translational activity of HCV IRESs cloned from six patients, whether responders or not to combination therapy, were conserved. We then tested the hypothesis that antisense molecules, i.e. short peptide nucleic acids (PNA), could inhibit HCV translation by binding to the highly conserved IIId or IV loop regions of the IRES. Five 6-10mer PNAs were designed. They strongly inhibit HCV IRES-driven translation in a rabbit reticulocyte lysate assay. This inhibition was highly specific since corresponding PNAs with only one mismatch were inactive. Short phosphorothioate oligonucleotides of same sequence were unable to inhibit HCV translation. PNA molecule was shown to have anti-HCV activity in Huh-7.5 cells when electroporated with a full-length HCV genome construct. Using oligonucleotide as carrier, PNA was also transfected in HCV replicon-harboring cells and in JFH1 infected Huh-7.5 cells.

journal_name

Antiviral Res

journal_title

Antiviral research

authors

Alotte C,Martin A,Caldarelli SA,Di Giorgio A,Condom R,Zoulim F,Durantel D,Hantz O

doi

10.1016/j.antiviral.2008.06.011

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

280-7

issue

3

eissn

0166-3542

issn

1872-9096

pii

S0166-3542(08)00330-6

journal_volume

80

pub_type

杂志文章

相关文献

ANTIVIRAL RESEARCH文献大全
  • Packaging signals in the 5'-ends of influenza virus PA, PB1, and PB2 genes as potential targets to develop nucleic-acid based antiviral molecules.

    abstract：:In a previous study a 15-mer phosphorothioate oligonucleotide (S-ON) derived from the packaging signal in the 5' end of segment 1 (PB2) of influenza A virus (designated 5-15b) proved markedly inhibitory to virus replication. Here we investigated whether analogous inhibitory S-ONs targeting the 5' end of segments 2 (PB...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2011.06.013

    authors： Giannecchini S,Wise HM,Digard P,Clausi V,Del Poggetto E,Vesco L,Puzelli S,Donatelli I,Azzi A

    更新日期：2011-10-01 00:00:00

  • Role of trehalose dimycolate-induced interferon-alpha/beta in the restriction of encephalomyocarditis virus growth in vivo and in peritoneal macrophage cultures.

    abstract：:Preventive intraperitoneal trehalose dimycolate (TDM) treatment of mice, inoculated with encephalomyocarditis (EMC) virus by the same route, caused restriction of virus growth in the peritoneum, which was correlated to IFN production in peritoneal fluids prior to infection. Peritoneal macrophages from TDM-treated mice...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(95)00047-p

    authors： Guillemard E,Geniteau-Legendre M,Kergot R,Lemaire G,Petit JF,Labarre C,Quero AM

    更新日期：1995-10-01 00:00:00

  • Impact of RNA polymerase I inhibitor CX-5461 on viral kinase-dependent and -independent cytomegalovirus replication.

    abstract：:Human cytomegalovirus (HCMV) infections cause congenital birth defects and disease in immunosuppressed individuals. Antiviral compounds can control infection yet their use is restricted due to concerns of toxicity and the emergence of drug resistant strains. We have evaluated the impact of an RNA Polymerase I (Pol I) ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2018.02.014

    authors： Westdorp KN,Terhune SS

    更新日期：2018-05-01 00:00:00

  • Anti-herpes simplex virus (HSV-1) activity of oxyresveratrol derived from Thai medicinal plant: mechanism of action and therapeutic efficacy on cutaneous HSV-1 infection in mice.

    abstract：:Oxyresveratrol, a major compound purified from Artocarpus lakoocha, a Thai traditional medicinal plant, was evaluated for its mechanism of action and therapeutic efficacy on cutaneous herpes simplex virus (HSV) infection in mice. The inhibitory concentrations for 50% HSV-1 plaque formation of oxyresveratrol, three cli...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2008.05.002

    authors： Chuanasa T,Phromjai J,Lipipun V,Likhitwitayawuid K,Suzuki M,Pramyothin P,Hattori M,Shiraki K

    更新日期：2008-10-01 00:00:00

  • Inhibitory effect of cinnamaldehyde, derived from Cinnamomi cortex, on the growth of influenza A/PR/8 virus in vitro and in vivo.

    abstract：:We have investigated the inhibitory effect of trans-cinnamaldehyde (CA), one of the principal constituents of essential oil derived from Cinnamomi cortex, on the growth of influenza A/PR/8 virus in vitro and in vivo. When 1-h drug treatment was initiated at various times post-infection (p.i.) in Madin-Darby canine kid...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2007.01.003

    authors： Hayashi K,Imanishi N,Kashiwayama Y,Kawano A,Terasawa K,Shimada Y,Ochiai H

    更新日期：2007-04-01 00:00:00

  • Genetic susceptibility in chronic viral hepatitis.

    abstract：:Infection with hepatitis B virus (HBV) or hepatitis C virus (HCV) may result in a number of different clinical outcomes. There is strong evidence in HBV infection that host genetic factors play a major role in determining the outcome of infection. A number of approaches may be used to determine the specific genetic fa...

    journal_title：Antiviral research

    pub_type： 杂志文章,评审

    doi：10.1016/s0166-3542(01)00175-9

    authors： Thursz M

    更新日期：2001-11-01 00:00:00

  • Preferential selection of heterologous G3-VP7 gene in the genetic background of simian rotavirus SA11 detected by using a homotypic single-VP7 gene-substitution reassortant.

    abstract：:Introduction of segmented genomes into virion is an important process in viral replication of rotavirus. We previously studied the assortment of the VP7 gene segment (encoding outer capsid protein VP7) in the genetic background of simian rotavirus SA11 (G serotype 3, G3) and found the preferential selection of homolog...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/s0166-3542(98)00006-0

    authors： Okada J,Kobayashi N,Taniguchi K,Urasawa S

    更新日期：1998-04-01 00:00:00

  • Complete inhibition of viral breakthrough by combination of MKC-442 with AZT during a long-term culture of HIV-1 infected cells.

    abstract：:We have investigated viral breakthrough during a long-term culture of HIV-1-infected cells with the non-nucleoside reverse transcriptase inhibitors (NNRTIs) 6-benzyl-1-ethoxymethyl-5-isopropyluracil (MKC-442), nevirapine and loviride (alpha-APA). When the compounds were examined for their inhibitory effects on HIV-1 (...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(96)00946-1

    authors： Okamoto M,Makino M,Yamada K,Nakade K,Yuasa S,Baba M

    更新日期：1996-06-01 00:00:00

  • Semi-quantitative detection of viral RNA in influenza A virus-infected mice for evaluation of antiviral compounds.

    abstract：:The aim of the study was to establish a murine model for sensitive screening of potential compounds with in vitro anti-influenza A virus activity. The evaluation in this in vivo model is based on semi-quantitative detection of viral RNA using one-step reverse transcriptase polymerase chain reaction (RT-PCR). After int...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/s0166-3542(02)00184-5

    authors： Sauerbrei A,Ulbricht A,Wutzler P

    更新日期：2003-03-01 00:00:00

  • Strand transfer inhibitors of HIV-1 integrase: bringing IN a new era of antiretroviral therapy.

    abstract：:HIV-1 integrase (IN) is one of three essential enzymes (along with reverse transcriptase and protease) encoded by the viral pol gene. IN mediates two critical reactions during viral replication; firstly 3'-end processing (3'EP) of the double-stranded viral DNA ends and then strand transfer (STF) which joins the viral ...

    journal_title：Antiviral research

    pub_type： 历史文章,杂志文章,评审

    doi：10.1016/j.antiviral.2009.11.004

    authors： McColl DJ,Chen X

    更新日期：2010-01-01 00:00:00

  • Treatment of herpes simplex virus infections.

    abstract：:Herpes simplex virus (HSV) types 1 and 2 are ubiquitous organisms that cause infections in human populations throughout the world. The clinical manifestations of HSV infections are varied, ranging from asymptomatic disease to life-threatening illness in neonates and immunocompromised hosts. This article will review th...

    journal_title：Antiviral research

    pub_type： 杂志文章,评审

    doi：10.1016/j.antiviral.2003.09.006

    authors： Brady RC,Bernstein DI

    更新日期：2004-02-01 00:00:00

  • Physical and biological characterization of antigen presenting liposome formulations: relative efficacy for the treatment of recurrent genital HSV-2 in guinea pigs.

    abstract：:Antigen presenting liposomes (APLs) containing both liposome encapsulated (44%) and free (56%) recombinant glycoprotein D of herpes simplex virus type-2 (rgD-2) were characterized with respect to the interaction of the antigen with the lipid bilayer and the biological activities provided by each form of rgD-2. We foun...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(90)90037-8

    authors： Ho RJ,Burke RL,Merigan TC

    更新日期：1990-04-01 00:00:00

  • Mycophenolic acid potently inhibits rotavirus infection with a high barrier to resistance development.

    abstract：:Rotavirus infection has emerged as an important cause of complications in organ transplantation recipients. Immunosuppressants used to prevent alloreactivity can also interfere with virus infection, but the direct effects of the specific type of immunosuppressants on rotavirus infection are still unclear. Here we prof...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2016.07.017

    authors： Yin Y,Wang Y,Dang W,Xu L,Su J,Zhou X,Wang W,Felczak K,van der Laan LJ,Pankiewicz KW,van der Eijk AA,Bijvelds M,Sprengers D,de Jonge H,Koopmans MP,Metselaar HJ,Peppelenbosch MP,Pan Q

    更新日期：2016-09-01 00:00:00

  • A cell-based, infectious-free, platform to identify inhibitors of lassa virus ribonucleoprotein (vRNP) activity.

    abstract：:The mammarenavirus Lassa (LASV) is highly prevalent in West Africa where it infects several hundred thousand individuals annually resulting in a high number of Lassa fever (LF) cases, a febrile disease associated with high morbidity and significant mortality. Mounting evidence indicates that the worldwide-distributed ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2019.104667

    authors： Cubitt B,Ortiz-Riano E,Cheng BY,Kim YJ,Yeh CD,Chen CZ,Southall NOE,Zheng W,Martinez-Sobrido L,de la Torre JC

    更新日期：2020-01-01 00:00:00

  • Antiviral activity of S-adenosylhomocysteine hydrolase inhibitors against plant viruses.

    abstract：:Three SAH hydrolase inhibitors, (RS)-3-adenin-9-yl-2-hydroxypropanoic acid (isobutyl ester) [(RS)-AHPA]; (RS)-9-(2,3-dihydroxypropyl)adenine [(RS)-DHPA] and the carbocyclic analog of 3-deazaadenosine (C-c3Ado) were evaluated for their inhibitory activity against tobacco mosaic virus (TMV) and potato virus X (PVX). Usi...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(90)90067-h

    authors： De Fazio G,Alba AP,Vicente M,De Clercq E

    更新日期：1990-05-01 00:00:00

  • Mode of action of 2-furylmercury chloride, an anti-rhinovirus compound.

    abstract：:2-Furylmercury chloride (2-FMC), an organic mercury derivative, has been found to inhibit the replication of all tested human rhinovirus (HRV) serotypes belonging to the antiviral group B and a limited number of HRV serotypes belonging to the antiviral group A. The mechanism of action of 2-FMC was tested against HRV-2...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2003.10.004

    authors： Verheyden B,Andries K,Rombaut B

    更新日期：2004-03-01 00:00:00

  • Impact of single nucleotide polymorphisms in the essential HCV entry factor CD81 on HCV infectivity and neutralization.

    abstract：:End stage liver disease caused by chronic infection with the hepatitis C virus (HCV) is a leading indication for liver transplantation, yet outcomes are poor since the liver graft is rapidly re-infected by HCV. Antibodies against the essential HCV receptor CD81 have been shown to inhibit HCV cell entry in vitro and in...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2013.10.018

    authors： Deest M,Westhaus S,Steinmann E,Manns MP,von Hahn T,Ciesek S

    更新日期：2014-01-01 00:00:00

  • In silico structure-based design and synthesis of novel anti-RSV compounds.

    abstract：:Respiratory syncytial virus (RSV) is the major cause for respiratory tract disease in infants and young children. Currently, no licensed vaccine or a selective antiviral drug against RSV infections are available. Here, we describe a structure-based drug design approach that led to the synthesis of a novel series of zi...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2015.08.003

    authors： Cancellieri M,Bassetto M,Widjaja I,van Kuppeveld F,de Haan CA,Brancale A

    更新日期：2015-10-01 00:00:00

  • Efficacy of pritelivir and acyclovir in the treatment of herpes simplex virus infections in a mouse model of herpes simplex encephalitis.

    abstract：:Pritelivir, a helicase-primase inhibitor, has excellent in vitro and in vivo activity against human herpes simplex virus (HSV). Mice lethally infected with HSV type 1 or 2, including acyclovir-resistant strains, were treated 72 h after infection for 7 days with pritelivir or acyclovir. Both drugs were administered ora...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2017.11.002

    authors： Quenelle DC,Birkmann A,Goldner T,Pfaff T,Zimmermann H,Bonsmann S,Collins DJ,Rice TL,Prichard MN

    更新日期：2018-01-01 00:00:00

  • Brain injury caused by HIV protease inhibitors: role of lipodystrophy and insulin resistance.

    abstract：:HIV-associated neurocognitive disorders (HAND) remain prevalent even with widespread use of combination antiretroviral therapy (ART), suggesting a potential role for co-morbidities in neurologic decline. Indeed, it is well established that ART drugs, particularly HIV protease inhibitors, can induce hyperlipidemia, lip...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2012.04.010

    authors： Gupta S,Knight AG,Losso BY,Ingram DK,Keller JN,Bruce-Keller AJ

    更新日期：2012-07-01 00:00:00

  • Recombinant full-length human cytomegalovirus protease has lower activity than recombinant processed protease domain in purified enzyme and cell-based assays.

    abstract：:Herpesviruses encode a protease that is essential for virus replication. The protease undergoes cleavage to a processed form during capsid maturation. A recombinant 75 kDa form of the protease from human cytomegalovirus was purified and compared with the recombinant 29 kDa processed form. Modification with an active s...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/s0166-3542(02)00051-7

    authors： Wittwer AJ,Funckes-Shippy CL,Hippenmeyer PJ

    更新日期：2002-08-01 00:00:00

  • Long-term inhibition of HIV-1 replication with RNA interference against cellular co-factors.

    abstract：:In this study we tested whether HIV-1 replication could be inhibited by stable RNAi-mediated knockdown of cellular co-factors. Cell lines capable of expressing shRNAs against 30 candidate co-factors implicated at different steps of the viral replication cycle were generated and analyzed for effects on cell viability a...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2010.11.005

    authors： Eekels JJ,Geerts D,Jeeninga RE,Berkhout B

    更新日期：2011-01-01 00:00:00

  • Maraviroc Clinical Test (MCT) as an alternative tool to decide CCR5-antagonists prescription in naïve HIV-infected patients.

    abstract：:Our aim was to analyze the virological response to a combined antiretroviral therapy started after Maraviroc Clinical Test (MCT) in naïve HIV-infected patients. Forty-one patients were exposed to MCT, based on an 8-day MVC monotherapy. If undetectability or a viral load reduction >1 log10 HIV-RNA copies/ml was achieve...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2015.06.018

    authors： Genebat M,de Pablo-Bernal RS,Pulido I,Jiménez-Mejías ME,Martínez O,Pacheco YM,Raffi-El-Idrissi Benhia M,Abad MA,Ruiz-Mateos E,Leal M

    更新日期：2015-09-01 00:00:00

  • Use of preconditioned human phagocytes for extracorporeal adsorption of viruses.

    abstract：:Conventional treatment of severe viral disease is limited by the narrow choice as well as the often-significant side effects or lack of clear efficacy of antiviral chemotherapy. At the same time, however, it is known that a reduction in viral load leads to significant clinical improvement in a number of important vira...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2010.05.007

    authors： Oberender F,Oberender H,Altrichter J,Reisinger EC,Mitzner SR

    更新日期：2010-08-01 00:00:00

  • Susceptibility of human influenza viruses from Australasia and South East Asia to the neuraminidase inhibitors zanamivir and oseltamivir.

    abstract：:Human influenza viruses isolated from Australasia (Australia and New Zealand) and South East Asia were analysed to determine their sensitivity to the NA inhibitor drugs, zanamivir and oseltamivir. A total of 532 strains isolated between 1998 and 2002 were tested using a fluorescence-based assay to measure the relative...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2003.11.008

    authors： Hurt AC,Barr IG,Hartel G,Hampson AW

    更新日期：2004-04-01 00:00:00

  • In vitro anti-influenza A activity of interferon (IFN)-λ1 combined with IFN-β or oseltamivir carboxylate.

    abstract：:Influenza viruses, which can cross species barriers and adapt to new hosts, pose a constant potential threat to human health. The influenza pandemic of 2009 highlighted the rapidity with which an influenza virus can spread worldwide. Currently available antivirals have a number of limitations against influenza, and no...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2014.09.008

    authors： Ilyushina NA,Donnelly RP

    更新日期：2014-11-01 00:00:00

  • The non-pathogenic Henipavirus Cedar paramyxovirus phosphoprotein has a compromised ability to target STAT1 and STAT2.

    abstract：:Immune evasion by the lethal henipaviruses, Hendra (HeV) and Nipah virus, is mediated by its interferon (IFN) antagonist P gene products, phosphoprotein (P), and the related V and W proteins, which can target the signal transducer and activator of transcription 1 (STAT1) and STAT2 proteins to inhibit IFN/STAT signalin...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2015.09.017

    authors： Lieu KG,Marsh GA,Wang LF,Netter HJ

    更新日期：2015-12-01 00:00:00

  • The ReFRAME library as a comprehensive drug repurposing library to identify mammarenavirus inhibitors.

    abstract：:Several mammarenaviruses, chiefly Lassa virus (LASV) in Western Africa and Junín virus (JUNV) in the Argentine Pampas, cause severe disease in humans and pose important public health problems in their endemic regions. Moreover, mounting evidence indicates that the worldwide-distributed mammarenavirus lymphocytic chori...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/j.antiviral.2019.104558

    authors： Kim YJ,Cubitt B,Chen E,Hull MV,Chatterjee AK,Cai Y,Kuhn JH,de la Torre JC

    更新日期：2019-09-01 00:00:00

  • Polymerases of hepatitis C viruses and flaviviruses: structural and mechanistic insights and drug development.

    abstract：:The family Flaviviridae comprises several major human pathogens including hepatitis C virus (genus hepacivirus), yellow fever virus, West Nile virus and dengue virus (genus flavivirus). Flaviviridae genomes comprise a single-stranded RNA segment encoding a single polyprotein that is subsequently processed into 10 matu...

    journal_title：Antiviral research

    pub_type： 杂志文章,评审

    doi：10.1016/j.antiviral.2014.02.006

    authors： Caillet-Saguy C,Lim SP,Shi PY,Lescar J,Bressanelli S

    更新日期：2014-05-01 00:00:00

  • Antiviral action of 5-amino-2-(2-dimethyl-aminoethyl)benzo-[de]-isoquinolin-1,3-dion e.

    abstract：:A newly synthesized imide derivative of 3-nitro-1,8-naphthalic acid, 5-amino-2-(2-dimethylaminoethyl)benzo-[de]-isoquinolin-1,3-dione (designated M-FA-142), was tested on chick embryo cells against herpes simplex virus type 1 (HSV-1) and vaccinia virus (VV), and on Vero cells against African swine fever virus (ASFV). ...

    journal_title：Antiviral research

    pub_type： 杂志文章

    doi：10.1016/0166-3542(84)90018-4

    authors： García Gancedo A,Gil-Fernández C,Vilas P,Pérez S,Rodriguez F,Paez E,Braña MF,Roldán CM

    更新日期：1984-08-01 00:00:00