Abstract:
:In this study we tested whether HIV-1 replication could be inhibited by stable RNAi-mediated knockdown of cellular co-factors. Cell lines capable of expressing shRNAs against 30 candidate co-factors implicated at different steps of the viral replication cycle were generated and analyzed for effects on cell viability and inhibition of HIV-1 replication. For half of these candidate co-factors we obtained knockdown cell lines that are less susceptible to virus replication. For three co-factors (ALIX, ATG16 and TRBP) the cell lines were resistant to HIV-1 replication for up to 2 months. With these cells we could test the hypothesis that HIV-1 is not able to escape from RNAi-mediated suppression of cellular co-factors, which was indeed not detected.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Eekels JJ,Geerts D,Jeeninga RE,Berkhout Bdoi
10.1016/j.antiviral.2010.11.005subject
Has Abstractpub_date
2011-01-01 00:00:00pages
43-53issue
1eissn
0166-3542issn
1872-9096pii
S0166-3542(10)00807-7journal_volume
89pub_type
杂志文章abstract:BACKGROUND AND AIMS:Interferon-alpha treatment has been the treatment of choice for chronic hepatitis with unpredictable results. Recently, Lamivudine has been licensed for use against HBV infection with good results. Unfortunately, recurrence of viremia after lamivudine withdrawal is common and prolonged treatment can...
journal_title:Antiviral research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0166-3542(00)00141-8
更新日期:2001-02-01 00:00:00
abstract::Influenza virus continues to remain one of the leading human respiratory pathogens causing significant morbidity and mortality around the globe. Due to short-term life cycle and high rate of mutations influenza virus is able to rapidly develop resistance to clinically available antivirals. This makes necessary the sea...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2015.06.004
更新日期:2015-08-01 00:00:00
abstract::Enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16) are the two major causative agents of hand, foot and mouth disease (HFMD), for which there are currently no licenced treatments. Here, the acquisition of resistance towards two novel capsid-binding compounds, NLD and ALD, was studied and compared to the analogous co...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2015.10.006
更新日期:2015-12-01 00:00:00
abstract::A screening for inhibitors of human immunodeficiency virus type 1 (HIV-1) among various types of isopolyoxomolybdates and heteropolyoxomolybdates was carried out by using an in vitro assay system measuring the cytopathogenicity of HIV-1 in CD4+ human MT-4 cells. A novel heteropolyoxomolybdate named PM-104 with the che...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(93)90075-t
更新日期:1993-04-01 00:00:00
abstract::Classical swine fever virus (CSFV) is the causative pathogen of classical swine fever (CSF), a highly contagious disease of swine. Mx proteins are interferon-induced dynamin-like GTPases present in all vertebrates with a wide range of antiviral activities. Although Zhao et al. (2011) have reported that human MxA can i...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2014.01.020
更新日期:2014-04-01 00:00:00
abstract::Human cytomegalovirus infection can lead to life-threatening clinical manifestations particularly in the immunocompromised host. Current therapy options face severe limitations leading to a continued search for alternative drug candidates. Viral replication is dependent on a balanced interaction between viral and cell...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2013.04.017
更新日期:2013-08-01 00:00:00
abstract::Wedelolactone (WDL) is a coumestan present in the plants Eclipta prostrata and Wedelia calendulacea which are used for treatment of a multitude of health problems in traditional medicine. It has previously been shown that WDL exerts antiviral activity against human immunodeficiency virus and hepatitis C virus. In this...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2019.104677
更新日期:2020-02-01 00:00:00
abstract::The ability to rescue an infectious, recombinant, negative-stranded, RNA virus from a complementary DNA (cDNA) clone, has led to new opportunities for measuring viral replication from a viral expressed reporter gene. In this study, the enhanced green fluorescent protein (EGFP) gene was inserted into the human parainfl...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2009.01.001
更新日期:2009-04-01 00:00:00
abstract::Infection with hepatitis B virus (HBV) or hepatitis C virus (HCV) may result in a number of different clinical outcomes. There is strong evidence in HBV infection that host genetic factors play a major role in determining the outcome of infection. A number of approaches may be used to determine the specific genetic fa...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/s0166-3542(01)00175-9
更新日期:2001-11-01 00:00:00
abstract::Human cytomegalovirus (HCMV) infections cause congenital birth defects and disease in immunosuppressed individuals. Antiviral compounds can control infection yet their use is restricted due to concerns of toxicity and the emergence of drug resistant strains. We have evaluated the impact of an RNA Polymerase I (Pol I) ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.02.014
更新日期:2018-05-01 00:00:00
abstract::Lactoferrin (LF), a member of the transferrin family, is a bi-globular protein secreted in milk, saliva, tears, seminal fluid, endocervix and vaginal secretions. LF is an important player in the defence against pathogenic microorganisms and has also been shown to have activity against several viruses including herpesv...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2004.05.005
更新日期:2004-10-01 00:00:00
abstract::Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hoofed animals. We have reported (Cubillos et al., 2008) that a synthetic dendrimeric peptide consisting of four copies of a B-cell epitope [VP1(136-154)] linked through thioether bonds to a T-cell epitope [3A(21-35)] of FMDV [B4T(thi)] e...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.03.005
更新日期:2016-05-01 00:00:00
abstract::We studied the effects of two antiviral agents, human interferon-alpha (IFN-alpha) and ribavirin, on subacute sclerosing panencephalitis (SSPE) virus infections in hamsters. By intracranial administration, IFN-alpha alone improved the survival of infected hamsters by 20% at a dose of 6 x 10(4) IU/kg every other day fo...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(97)00055-7
更新日期:1998-01-01 00:00:00
abstract::The effect of interferon on the replication of herpes simplex virus types 1 and 2 was studied in two murine macrophage-like cell lines which differ in their ability to synthesize interferon. Higher titers of herpes simplex virus type 1 occurred in PU5-1.8 cell cultures where interferon was not produced than in J774A.1...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(86)90010-0
更新日期:1986-05-01 00:00:00
abstract::The antiviral effect of foscarnet (PFA) on the replication of rotavirus, a member of the Reoviridae, was studied. The pyrophosphate analogue is an effective inhibitor of several viral polymerases acting on the enzyme pyrophosphate binding site. Replication of rotavirus in MA104 cells using different u.o.i. was inhibit...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(94)00085-m
更新日期:1995-05-01 00:00:00
abstract::Dextran sulfate (DS) is a negatively charged sulfated polysaccharide that suppresses the replication of influenza A viruses. The suppression was thought to be associated with inhibition of the hemagglutinin-dependent fusion activity. However, we previously showed that suppression by DS was observed not only at the ini...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2014.09.002
更新日期:2014-11-01 00:00:00
abstract::In the scope of the search for new anti-HIV agents interacting with a new target, we developed a high-throughput screening system to detect the interactions between Nef and Hck. Nef is an accessory protein of HIV, which is involved in the pathogenicity of the acquired immunodeficiency syndrome (AIDS). Nef is also a si...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(02)00017-7
更新日期:2002-07-01 00:00:00
abstract::Several polyanionic compounds with potential for use as topically applied microbicides to prevent HIV-1 sexual transmission, such as PRO 2000, are currently in phase III clinical efficacy trials. Microbicidal formulations may well comprise combinations of inhibitors to increase potency, reduce dose and minimize proble...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2007.03.004
更新日期:2007-09-01 00:00:00
abstract::Stavudine (d4T) is used extensively as part of HAART in resource poor settings, despite its toxicities. The revised WHO guidelines specify replacement of d4T with less toxic but more expensive drugs when feasible, and that d4T doses be standardized to 30 mg twice daily (bid) (irrespective of body-weight), from the app...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2011.08.004
更新日期:2011-11-01 00:00:00
abstract::The hepatitis B virus nucleocapsid or core antigen is extremely immunogenic during infection and after immunization. This review summarizes several features of the nucleocapsid which explain this exceptionally high immunogenicity: a unique three-dimensional folding, the presence of a region that interacts with immunog...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2003.08.011
更新日期:2003-10-01 00:00:00
abstract::Several arenaviruses cause hemorrhagic fever (HF) in humans, and evidence indicates that the worldwide-distributed prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) is a neglected human pathogen of clinical significance. Moreover, arenaviruses pose a biodefense threat. No licensed anti-arenavirus vaccine...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2008.08.002
更新日期:2008-12-01 00:00:00
abstract::Acute lower respiratory tract infections (ALRI) caused by respiratory syncytial virus (RSV) are associated with a severe disease burden among infants and elderly patients. Treatment options are limited. While numerous drug candidates with different viral targets are under development, the utility of RSV entry inhibito...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2020.104774
更新日期:2020-05-01 00:00:00
abstract:BACKGROUND:By modulating the antiviral immune response via vitamin D receptor, the active form of vitamin D (1,25-dihydroxyvitamin D, calcitriol) could play a central role in protection against respiratory virus infections. This in vitro study tested the hypothesis that respiratory viruses modulate vitamin D receptor e...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.11.004
更新日期:2017-01-01 00:00:00
abstract::Foot and mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals. Previously, we found that the epitope peptide EP141-160 displayed on virus-like particles (VLP) for use as a vaccine showed high immunoreactivity and conferred partially effective protection to animals. In this study, we first ...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2016.08.020
更新日期:2016-10-01 00:00:00
abstract::Therapeutic strategies controlling human cytomegalovirus (hCMV) infection are limited due to adverse side effects and emergence of antiviral resistance variants. A compound being evaluated for treating hCMV disease is maribavir (MBV) which disrupts replication by inhibiting the viral kinase pUL97. Previous studies hav...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2013.09.011
更新日期:2013-11-01 00:00:00
abstract::Dengue virus (DENV) infection is the most prevalent mosquito-borne viral infection of which there is no licensed therapeutic drug available. Previous studies have shown that minocycline, an antibiotic, can inhibit DENV infection in vitro. However, the mechanism is not fully understood. It is known that macrophage migr...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.05.002
更新日期:2018-07-01 00:00:00
abstract::Human CMV causes a number of diseases that cause considerable morbidity and that can be life-threatening in immunocompromised patients, particularly those with AIDS. Ganciclovir (GCV) and Foscarnet (PFA) are currently the drugs of choice for management of CMV disease. Both are not without side effects and have a relat...
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(94)90048-5
更新日期:1994-05-01 00:00:00
abstract::Japanese encephalitis virus (JEV) infection is a major cause of acute viral encephalitis both in humans and animals. The domain III of virus envelope protein (EDIII) plays important roles in interacting with host cell receptors to facilitate virus entry. In this study, recombinant JEV EDIII was expressed and purified....
journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2012.03.002
更新日期:2012-05-01 00:00:00
abstract::Nipah and Hendra virus are members of a newly identified genus of emerging paramyxoviruses, the henipaviruses. Both viruses have the ability to cause severe pulmonary infection and severe acute encephalitis. Following their discovery in the 1990s, outbreaks caused by these zoonotic paramyxoviruses have been associated...
journal_title:Antiviral research
pub_type: 杂志文章,评审
doi:10.1016/j.antiviral.2012.05.008
更新日期:2012-08-01 00:00:00
abstract::Salvage therapy with ritonavir (RTV) and saquinavir (SQV) failed to achieve virological and immunological improvement in 24 HIV-infected patients who discontinued triple therapy with RTV or indinavir (IDV) because of failure or intolerance to treatment. Changes in the HIV-1 protease gene sequence were analyzed prospec...
journal_title:Antiviral research
pub_type: 临床试验,杂志文章
doi:10.1016/s0166-3542(00)00110-8
更新日期:2000-09-01 00:00:00