Down-regulation of suppressor of cytokine signaling 3 by miR-122 enhances interferon-mediated suppression of hepatitis B virus.


:MicroRNA-122 (miR-122) is involved in the pathogenesis of several liver diseases, including chronic hepatitis B infection and hepatocellular carcinoma. This study aimed to explore the potential role of miR-122 in the interferon (IFN)-mediated suppression of hepatitis B virus (HBV) in hepatocytes. We found that elevated expression of suppressor of cytokine signaling 3 (SOCS3) following HBV infection, contributed to the inactivation of the IFN signaling pathway. Based on previous studies from our laboratory showing that miR-122 can modulate type I IFN expression by inhibiting SOCS1 expression, we analyzed the SOCS3 mRNA sequence for putative miR-122 binding sites. We demonstrate that miR-122 inhibits SOCS3 expression by targeting the 3'-untranslated region of the SOCS3 mRNA within the region 1887-1910 nucleotides. Finally, we demonstrate that significantly increased levels of IFN lead to decreased HBV expression in miR-122 mimic-treated Huh7 cells, whereas inhibition of endogenous miR-122 leads to enhanced viral production, owing to a marked decrease in IFN expression. Taken together, our results demonstrate that miR-122 down-regulates SOCS3, thus positively affecting the anti-HBV efficiency of endogenous type I IFN. Our study suggests that suppression of miR-122 induced by HBV infection, leads to the inactivation of IFN expression, which in turn enhances HBV replication, contributing to viral persistence and hepatocarcinogenesis.


Antiviral Res


Antiviral research


Gao D,Zhai A,Qian J,Li A,Li Y,Song W,Zhao H,Yu X,Wu J,Zhang Q,Kao W,Wei L,Zhang F,Zhong Z




Has Abstract


2015-06-01 00:00:00












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    journal_title:Antiviral research

    pub_type: 杂志文章


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    pub_type: 杂志文章,评审


    authors: Holz L,Rehermann B

    更新日期:2015-02-01 00:00:00

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    pub_type: 杂志文章


    authors: Genebat M,de Pablo-Bernal RS,Pulido I,Jiménez-Mejías ME,Martínez O,Pacheco YM,Raffi-El-Idrissi Benhia M,Abad MA,Ruiz-Mateos E,Leal M

    更新日期:2015-09-01 00:00:00

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    journal_title:Antiviral research

    pub_type: 杂志文章


    authors: Sauerbrei A,Deinhardt S,Zell R,Wutzler P

    更新日期:2010-06-01 00:00:00