Abstract:
:MicroRNA-122 (miR-122) is involved in the pathogenesis of several liver diseases, including chronic hepatitis B infection and hepatocellular carcinoma. This study aimed to explore the potential role of miR-122 in the interferon (IFN)-mediated suppression of hepatitis B virus (HBV) in hepatocytes. We found that elevated expression of suppressor of cytokine signaling 3 (SOCS3) following HBV infection, contributed to the inactivation of the IFN signaling pathway. Based on previous studies from our laboratory showing that miR-122 can modulate type I IFN expression by inhibiting SOCS1 expression, we analyzed the SOCS3 mRNA sequence for putative miR-122 binding sites. We demonstrate that miR-122 inhibits SOCS3 expression by targeting the 3'-untranslated region of the SOCS3 mRNA within the region 1887-1910 nucleotides. Finally, we demonstrate that significantly increased levels of IFN lead to decreased HBV expression in miR-122 mimic-treated Huh7 cells, whereas inhibition of endogenous miR-122 leads to enhanced viral production, owing to a marked decrease in IFN expression. Taken together, our results demonstrate that miR-122 down-regulates SOCS3, thus positively affecting the anti-HBV efficiency of endogenous type I IFN. Our study suggests that suppression of miR-122 induced by HBV infection, leads to the inactivation of IFN expression, which in turn enhances HBV replication, contributing to viral persistence and hepatocarcinogenesis.
journal_name
Antiviral Resjournal_title
Antiviral researchauthors
Gao D,Zhai A,Qian J,Li A,Li Y,Song W,Zhao H,Yu X,Wu J,Zhang Q,Kao W,Wei L,Zhang F,Zhong Zdoi
10.1016/j.antiviral.2015.03.001subject
Has Abstractpub_date
2015-06-01 00:00:00pages
20-8eissn
0166-3542issn
1872-9096pii
S0166-3542(15)00053-4journal_volume
118pub_type
杂志文章abstract::We examined 28 children with HIV-1 infection who were not responding to existing antiviral regimens and were enrolled into clinical trials conducted at the National Cancer Institute to receive salvage therapy. In 3 of the 28 patients (10.7%), the Q151M complex amino acid substitutions were identified. The three patien...
journal_title:Antiviral research
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abstract::In cultured mammalian cells (Vero), different antiviral agents change to differing degrees the ability of HSV2 to down-regulate gap junctions, each agent having a specific effect. Measured by intracellular electrodes, control cell populations showed 49-51% coupling, uninfected populations treated with acyclovir or SDS...
journal_title:Antiviral research
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pub_type: 杂志文章
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(94)00064-f
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journal_title:Antiviral research
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doi:10.1016/j.antiviral.2006.12.005
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2008.07.002
更新日期:2008-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0166-3542(00)00102-9
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2011.10.006
更新日期:2011-12-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2011.01.001
更新日期:2011-03-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2007.06.001
更新日期:2007-10-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2015.07.003
更新日期:2015-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/0166-3542(83)90010-4
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(94)90005-1
更新日期:1994-12-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(95)00038-n
更新日期:1995-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0166-3542(99)00074-1
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journal_title:Antiviral research
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doi:10.1016/j.antiviral.2015.08.003
更新日期:2015-10-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章,评审
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doi:10.1016/j.antiviral.2010.04.015
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2013.04.017
更新日期:2013-08-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/0166-3542(92)90086-k
更新日期:1992-01-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2018.04.017
更新日期:2018-07-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/j.antiviral.2007.05.004
更新日期:2007-10-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
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journal_title:Antiviral research
pub_type: 杂志文章
doi:10.1016/s0166-3542(00)00063-2
更新日期:2000-02-01 00:00:00
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journal_title:Antiviral research
pub_type: 杂志文章
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journal_title:Antiviral research
pub_type: 杂志文章,评审
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更新日期:2018-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0166-3542(96)00990-4
更新日期:1996-12-01 00:00:00