Abstract：
:Recent advances in genome engineering are starting a revolution in biological research and translational applications. The clustered regularly interspaced short palindromic repeats (CRISPR)-associated RNA-guided endonuclease CRISPR associated protein 9 (Cas9) and its variants enable diverse manipulations of genome function. In this review, we describe the development of Cas9 tools for a variety of applications in cell biology research, including the study of functional genomics, the creation of transgenic animal models, and genomic imaging. Novel genome engineering methods offer a new avenue to understand the causality between the genome and phenotype, thus promising a fuller understanding of cell biology.
journal_name
Trends Cell Bioljournal_title
Trends in cell biologyauthors
Wang F,Qi LSdoi
10.1016/j.tcb.2016.08.004subject
Has Abstractpub_date
2016-11-01 00:00:00pages
875-888issue
11eissn
0962-8924issn
1879-3088pii
S0962-8924(16)30121-0journal_volume
26pub_type
杂志文章,评审abstract：:Genome-wide analyses of yeast provide insight into cellular responses to reactive oxygen species (ROS). Many deletion mutants are sensitive to at least one ROS, but no one oxidant is representative of 'oxidative stress' despite the widespread use of a single compound such as H(2)O(2). This has major implications for s...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2005.04.003
更新日期：2005-06-01 00:00:00
abstract：:Evolutionary conserved and pleiotropic, the TPT1/TCTP gene (translationally controlled tumor protein, also called HRF, fortilin), encodes a highly structured mRNA shielded by ribonucleoproteins and closely resembling viral particles. This mRNA activates, as do viruses, protein kinase R (PKR). The TPT1/TCTP protein is ...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2012.10.002
更新日期：2013-01-01 00:00:00
abstract：:The nucleus is a spatially organized compartment. The most obvious way in which this is achieved is at the level of chromosomes. The positioning of chromosomes with respect to nuclear landmarks and with respect to each other is both non-random and cell-type specific. This suggests that cells possess molecular mechanis...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2011.08.001
更新日期：2011-12-01 00:00:00
abstract：:The contribution of extracellular matrix (ECM) components to the regulation of cell adhesion, proliferation and differentiation is receiving much attention. Recently, it has become evident that certain cellular responses require the combined action of ECM components and soluble growth factors. This article examines po...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/0962-8924(92)90057-t
更新日期：1992-03-01 00:00:00
abstract：:Endosomal protein sorting governs the fate of many physiologically important proteins involved in a panoply of cellular functions. Recent discoveries have revealed a vital role for endosomally localised branched actin patches in facilitating protein sorting. The formation of the actin patches has been shown to require...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2013.04.010
更新日期：2013-11-01 00:00:00
abstract：:The vascular system matures during embryonic development to form a stable, well-organized tubular network. In vivo data have established that the extracellular matrix (ECM) is crucial in providing structural support to the vascular system. In vitro studies are defining the involvement of ECM-smooth-muscle cell signali...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0962-8924(02)00007-7
更新日期：2003-01-01 00:00:00
abstract：:The anaphase-promoting complex/cyclosome (APC/C) is an evolutionarily conserved ubiquitin ligase that controls cell cycle progression through spatiotemporally regulated proteolysis. Although recent studies revealed its postmitotic function, our knowledge of the role of APC/C beyond cell cycle regulation in the biology...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2019.03.001
更新日期：2019-07-01 00:00:00
abstract：:Noncoding RNAs are emerging as central players in gene expression regulation through the targeting of a plethora of mRNAs. Several recent papers report on the regulation of the epithelial phenotype by the miR-200 (200 family of miRNAs) family and a natural antisense transcript, both acting on the E-cadherin repressors...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2008.05.005
更新日期：2008-08-01 00:00:00
abstract：:Microtubules are composed predominantly of two related proteins: alpha- and beta-tubulin. These proteins form the tubulin heterodimer, which is the basic building block of microtubules. Surprisingly, recent molecular genetic studies have revealed the existence of gamma-tubulin, a new member of the tubulin family. Like...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/0962-8924(92)90125-7
更新日期：1992-01-01 00:00:00
abstract：:It was once believed that common mechanisms underpin the transfer of proteins across the membrane systems of organelles such as mitochondria, chloroplasts and peroxisomes. Now that many of the core components of the translocases have been indentified, results discussed at a recent conference [Max-Delbrück-Centrum Symp...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/s0962-8924(01)02083-9
更新日期：2001-09-01 00:00:00
abstract：:To battle adverse internal and external conditions and maintain homeostasis, diploid organisms employ various cellular processes, such as proliferation and apoptosis. In some tissues, an alternative mechanism, endoreplication, is employed toward similar goals. Endoreplication is an evolutionarily conserved cell cycle ...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2018.02.006
更新日期：2018-06-01 00:00:00
abstract：:Solid tumors typically develop hostile microenvironments characterized by irregular vascularization and poor oxygen (O2) and nutrient supply. Whereas normal cells modulate anabolic and catabolic pathways in response to changes in nutrient availability, cancer cells exhibit unregulated growth even under nutrient scarci...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2014.06.001
更新日期：2014-08-01 00:00:00
abstract：:Endocytosis is a complicated yet highly efficient process that involves the uptake and processing of cargoes, ranging from small molecules, to activated signalling receptors, to whole microorganisms. Regulation of endocytic pathways is poorly understood. Recent evidence suggests that the Rho GTPase family of signallin...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0962-8924(99)01710-9
更新日期：2000-03-01 00:00:00
abstract：:Most published work on post-translational histone modifications focuses on small covalent alterations such as acetylation, methylation and phosphorylation. By contrast, fewer data are available on the modification of histones by ADP-ribose. Discussion of the biological significance of histone ADP-ribosylation has ofte...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2011.06.001
更新日期：2011-09-01 00:00:00
abstract：:The glycoproteome, the ensemble of glycans and their carrier proteins, plays major roles in multicellular life by regulating cell interactions with their environment. How information is encoded into the glycome, in other words how glycosylation is modulated in response to signals, remains largely unclear. Glycosylatio...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2015.12.004
更新日期：2016-05-01 00:00:00
abstract：:The inositol phospholipids (PIs) comprise a family of eight species with different combinations of phosphate groups arranged around the inositol ring. PIs are among the most versatile signaling molecules known, with key roles in receptor-mediated signal transduction, actin remodeling and membrane trafficking. Recent s...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2005.03.008
更新日期：2005-05-01 00:00:00
abstract：:The mitochondrial electron transport chain is the major source for the production of oxygen radicals. Mitochondria-generated reactive oxygen species (mROS) have been implicated in decreasing the life span and contributing to age-related diseases (known as the free radical theory of aging). Recently, the serine/threoni...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/j.tcb.2006.11.003
更新日期：2007-01-01 00:00:00
abstract：:Intercellular communication refers to the different ways through which cells communicate with each other and transfer a variety of messages. These communication methods involve a number of different processes that occur individually or simultaneously, which change depending on the physiological or pathological context...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2020.05.003
更新日期：2020-08-01 00:00:00
abstract：:Intracellular bacteria including major pathogens live in the cytoplasm or in cytoplasmic vacuoles within their host cell. However, some can invade more unusual intracellular niches such as the eukaryotic nucleus. Phylogenetically diverse intranuclear bacteria have been discovered in various protist, arthropod, marine ...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2015.01.002
更新日期：2015-06-01 00:00:00
abstract：:Ultraviolet (UV) irradiation injures the epidermis, resulting in sunburn and inflammation. UV-irradiated keratinocytes secrete interleukin-1beta through a caspase-1-dependent mechanism. In seeking a link between UV-irradiation and caspase-1 activation, a prominent role for the NOD-like receptor (NLR) family of innate ...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/j.tcb.2007.10.004
更新日期：2008-01-01 00:00:00
abstract：:Living organisms undergo biochemical, physiological and behavioral cycles with periods ranging from seconds to years. Cycles with intermediate periods are governed by endogenous clocks that depend on oscillating gene expression. Here we illustrate the modalities and specific functions of post-transcriptional control o...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2010.06.004
更新日期：2010-09-01 00:00:00
abstract：:A wealth of evidence underscores the tight link between oxidative stress, neurodegeneration and aging. When the level of excess reactive oxygen species (ROS) increases in the cell, a phenomenon characteristic of aging, DNA is damaged, proteins are oxidized, lipids are degraded and more ROS are produced, all culminatin...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2009.09.008
更新日期：2010-01-01 00:00:00
abstract：:Membrane trafficking pathways function to sort and transport cargoes to various intracellular compartments and to the plasma membrane. This allows precise spatiotemporal control of processes such as signal transduction, which in turn is crucial for complex cell functions such as cell division, migration and polarity. ...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2011.02.003
更新日期：2011-05-01 00:00:00
abstract：:Mounting evidence suggests that DNA damage plays a central role in aging. Multiple tiers of defense have evolved to reduce the accumulation of DNA damage, including reducing damaging molecules, repairing DNA damage, and inducing senescence or apoptosis in response to persistent DNA damage. Mutations in or failure of t...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2019.12.001
更新日期：2020-02-01 00:00:00
abstract：:Ran is an abundant GTPase that is highly conserved in eukaryotic cells and has been implicated in many aspects of nuclear structure and function, especially determining the directionality of nucleocytoplasmic transport during interphase. However, cell-free systems have recently shown that Ran plays distinct roles in m...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/s0962-8924(01)02071-2
更新日期：2001-09-01 00:00:00
abstract：:Members of the myosin-I family of molecular motors are expressed in many eukaryotes, where they are involved in a multitude of critical processes. Humans express eight distinct members of the myosin-I family, making it the second largest family of myosins expressed in humans. Despite the high degree of sequence conser...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2012.10.008
更新日期：2013-02-01 00:00:00
abstract：:A major challenge of developmental biology is to understand how cells coordinate developmental behaviors with their neighbors. To achieve this, cells often employ signaling molecules that emanate from a local source and act at a distance on target cells. The Hedgehog morphogen is an essential signaling molecule requir...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2010.12.005
更新日期：2011-04-01 00:00:00
abstract：:Microbiology has a long way to go. Microbes are ubiquitous, and all other life forms in the biosphere exist solely because of them, but, as less than 1% of microorganisms can be grown in the laboratory, more than a century of research has revealed only the tip of the iceberg concerning this most crucial of life scienc...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:Like the conductor of an orchestra, the Sec protein translocation channel is the platform needed to bring together the many different players required for the constitutive and obligatory process of protein transport. This conserved membrane channel, termed SecY in bacteria and Sec61 in eukaryotes, creates a ubiquitous...
journal_title：Trends in cell biology
pub_type： 杂志文章,评审
doi：10.1016/j.tcb.2011.04.005
更新日期：2011-09-01 00:00:00
abstract：:Diverse biological and developmental functions are mediated by signalling through the epidermal growth factor (EGF) receptor. In flies, many different mechanisms are used to control and restrict EGF-R signalling, including ligand processing, ligand variety (an inhibitor as well as activators), transcription and perhap...
journal_title：Trends in cell biology
pub_type： 杂志文章
doi：10.1016/S0962-8924(97)01143-4
更新日期：1997-11-01 00:00:00
