Abstract：
AIMS:Previous work has demonstrated that ketogenic diets promote white fat browning; however, the exact mechanisms underlying this phenomenom have yet to be elucidated. Recently, an in vitro study showed that supraphysiological concentrations of β-hydroxybutyrate (βHB) had a strong influence on the induction of adipocyte browning. On the other hand, concentrations in the physiological range, achieved through ketogenic diets and prolonged fasting produce values of 1-3 mM and 4-7 mM, respectively. Herein, we investigated the impact of physiological concentrations of βHB on metabolism, and the expression of uncoupling protein 1 (UCP1) and other browning markers in adipose tissues. MAIN METHODS:The effects of βHB on adipocyte browning were investigated in vitro, using primary cultures of isolated visceral and subcutaneous fat cells and cultured 3T3-L1 adipocytes, and in vivo. KEY FINDINGS:It was determined that βHB failed to induce changes in the oxidative capacity, citrate synthase activity or browning gene expression patterns in isolated adipocytes, and did not exert a permissive effect on β-adrenergic agonist-induced browning. In addition, 3T3-L1 adipocytes differentiated following βHB treatment exhibited downregulated Ucp1 expression levels, a result that was recapitulated in the subcutaneous adipose tissue of Wistar rats after βHB salt treatment. Rats administered βHB salts also presented reduced brown adipose tissue UCP1 protein expression. SIGNIFICANCE:The mechanisms underlying ketogenic diet-induced browning of adipocytes are not known. The results from the present study indicate that physiological concentrations of βHB are not responsible for this phenomenon, despite the observed βHB-mediated downregulation of UCP1 expression.
journal_name
Life Scijournal_title
Life sciencesauthors
de Oliveira Caminhotto R,Andreotti S,Komino ACM,de Fatima Silva F,Sertié RAL,Christoffolete MA,Reis GB,Lima FBdoi
10.1016/j.lfs.2019.116683subject
Has Abstractpub_date
2019-09-01 00:00:00pages
116683eissn
0024-3205issn
1879-0631pii
S0024-3205(19)30609-5journal_volume
232pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：AIMS:The aim of the present study was to evaluate the oral resveratrol effects associated with diet and physical training changes on anthropometric and biochemical parameters. MAIN METHODS:25 individuals aged from 30 to 60 years old; with Body Mass Index (BMI) ≥ 30 kg/m2 were included in the study. Following the prima...
journal_title：Life sciences
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.lfs.2020.117962
更新日期：2020-09-01 00:00:00
abstract：:Our previous biochemical and mechanical studies have demonstrated an increase in Ca2+ sensitivity of cardiac myofilaments in ovariectomized rats. To test whether the body weight gain associated with ovariectomy contributed some effects to the changes in myofibrillar functions, the relations of pCa (-log Ca2+ molar con...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00623-2
更新日期：2000-01-01 00:00:00
abstract：AIMS:We recently reported that acute exposure to nicotine vasodilates the renal vasculature of male rats via facilitation of endothelial nitric oxide synthase (eNOS). In this study, we investigated whether this effect of nicotine is sexually dimorphic and the role of estrogen in modulating the nicotine effect. MAIN ME...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.11.009
更新日期：2011-01-17 00:00:00
abstract：:The effect of cadmium (Cd2+) was studied in vitro on the flavokinase (ATP : riboflavin 5'-phosphotransferase, EC 2.7.1.26) activity purified from rat liver. Cadmium inhibited flavokinase activity in a concentration-dependent manner and the effect was completely reversed by increasing concentration of zinc (Zn2+), indi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00151-3
更新日期：1997-01-01 00:00:00
abstract：:Autophagy is an essential mechanism of cellular degradation, a way to protect the cells under stress conditions, such as deprivation of nutrients, growth factors and cellular damage. However, in normal physiology autophagy plays a significant role in cancer cells. Current research is in progress to understand how auto...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2020.118184
更新日期：2020-10-15 00:00:00
abstract：AIMS:Acetylcholine is synthesized in more or less all mammalian cells. However, little is known about the subcellular location of acetylcholine synthesis. Therefore, in the present experiments the subcellular location of the synthesizing enzyme choline acetyltransferase (ChAT) was investigated by anti-ChAT immunogold e...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.05.012
更新日期：2012-11-27 00:00:00
abstract：:The cellular electrophysiology of left ventricular preparations from guinea pig hearts was studied 1 hour, 24 hours, and 4-6 weeks after myocardial infarction produced by 6-8 single ties of the distal left coronary artery system or after sham operation. Microelectrode recordings were used to monitor cells from the end...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90343-1
更新日期：1985-06-24 00:00:00
abstract：:Cardiovascular risk factors associated with hypertension include alterations in arterial compliance and an increased tendency to thrombosis. In this study we examined the relationship between arterial compliance and endothelial derived components of the hemostatic system: von Willebrand factor (vWF) and tissue plasmin...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90192-6
更新日期：1993-01-01 00:00:00
abstract：:This in vitro study was conducted to determine whether tachyphylaxis of guinea pig airway to furosemide occurs under conditions that produce tachyphylaxis to the beta 2-adrenoceptor agonist, salbutamol. Isometric tension was measured in tracheal rings bathed in HEPES buffer from 4-6 d newborn guinea pigs of either sex...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00416-x
更新日期：1996-01-01 00:00:00
abstract：:The risk of atherosclerosis (AS) ascends among post-menopausal women, while current hormone replacement therapy exerts several adverse effects. Alisol B 23-acetate (AB23A), a tetracyclic triterpenoid isolated from the rhizome of Alisma orientale, was reported to show multiple physiological activities, including regula...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118030
更新日期：2020-10-01 00:00:00
abstract：:Systemic administration of the amphetamine analogue, 3,4-methylenedioxymethamphetamine (MDMA) produced a dose-dependent increase in the extracellular concentration of dopamine (DA) in the striatum as measured by in vivo microdialysis in awake, freely-moving rats. The extracellular concentration of the DA metabolite, 3...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90484-9
更新日期：1990-01-01 00:00:00
abstract：:The neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is rapidly metabolized to a 1-methyl-4-phenylpyridinium species (MPP+) in the squirrel monkey. After administration of toxic doses of MPTP, the concentration of MPP+ in the substantia nigra appears to increase during the first 72 hours, reaching the hi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90060-8
更新日期：1985-01-21 00:00:00
abstract：BACKGROUND:Most hepatocellular carcinoma cases are diagnosed at late stages of the disease, which makes it the second cause of cancer mortality worldwide. For advanced-stage patients, chemotherapeutic drugs are the best treatment option; however, their adverse effects and high cost are still major obstacles for effecti...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118827
更新日期：2021-01-15 00:00:00
abstract：:This study was designed to document possible changes in bupivacaine and its main metabolite, des-butyl-bupivacaine (PPX) kinetics in mice after a single 5 mg/kg injection of diltiazem. After a single 20 mg/kg i.p. dose of bupivacaine, kinetic parameters of bupivacaine were not statistically different but the ratio AUC...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00601-6
更新日期：1994-01-01 00:00:00
abstract：:beta-Adrenergic agonists used in therapy of premature labor and asthma cross the placenta and can affect development of the fetal nervous system. In the current study, pregnant rats were given 10 mg/kg of terbutaline on gestational days 17, 18 and 19 and adrenergic receptor binding capabilities examined in brain regio...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90440-3
更新日期：1990-01-01 00:00:00
abstract：:Glycated human serum albumin (glc HSA) and glc proteins in back hairs from diabetic rats were deglycated by incubation with hydrazine or aminoguanidine (guanylhydrazine) at physiological temperature (37 degrees C). Glc HSA decreased by 35% with 0.2 M hydrazine at day 20 and by 56% with 0.1 M aminoguanidine at day 10. ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90681-r
更新日期：1993-01-01 00:00:00
abstract：:The gastric mucosa is frequently exposed to different exogenous and endogenous ulcerative agents. Alcoholism is one of the risk factors for the development of mucosal damage in the stomach. This study aimed to assess if a probiotic strain Lactobacillus rhamnosus GG (LGG) is capable of protecting the gastric mucosa fro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.03.018
更新日期：2007-05-16 00:00:00
abstract：:The influence of prior incubation on [3H]tryptamine binding was investigated in rat brain synaptic plasma membranes. A 55 min preincubation of the membranes at 37 degrees C induced an approx. 2.4-fold increase in the specific binding of [3H]ligand to the subsequently washed preparations and this phenomenon was quite t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90428-5
更新日期：1986-04-07 00:00:00
abstract：:The vascular endothelium is recognized as a barrier between blood and blood vessel wall. The abnormality of vascular endothelium is critical for atherosclerosis, hypertension and diabetes. Oxidative stress, inflammation, obesity, hyperlipidemia and insulin resistance are major contributors to endothelial dysfunction i...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2016.11.005
更新日期：2016-12-15 00:00:00
abstract：:Groups of 35-day-old male C57BL/6 mice were stressed 1 hour per day by immobilization for 1, 2, 3, 5, 8, 11 or 14 consecutive days. Control groups were left undisturbed. The animals were then killed and body weight and the weights of the thymus, spleen and axillary lymph nodes determined. Chronic immobilization stress...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00606-1
更新日期：1997-01-01 00:00:00
abstract：AIM:Cadmium is a persistent ubiquitous environmental toxicant that elicits several biological defects on delicate body organs. Growing evidence suggests that cadmium (Cd) may perturb signaling pathways to induce oxidative pancreatitis. Thus, we explored whether hesperidin, a flavonone, could mitigate Cd-induced oxidati...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118268
更新日期：2020-10-15 00:00:00
abstract：:Rats, trained to discriminate 1.5 mg/kg of the hallucinogenic agent 5-methoxy-N,N-dimethyltryptamine (5-OMe DMT) from saline in a two-lever drug discrimination task, were challenged with various doses of the 4-methoxy, 4-methylthio and 5-methylthio derivatives of DMT. The 5-OMe DMT cue was found to generalize to all t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90463-5
更新日期：1982-02-01 00:00:00
abstract：:Pretreatment of pancreatic acini with 5-hydroxytryptamine (5-HT) reduced the binding of the labeled somatostatin (SS) analogue 125I-Tyr3-SMS to pancreatic acinar membranes. This effect was dependent of the dose of 5-HT used and length of pretreatment. This inhibitory effect of 5-HT was abolished when pancreatic acini ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00161-6
更新日期：1997-01-01 00:00:00
abstract：:Histidine decarboxylase (HDC) activity and histamine content were measured in endoscopic gastric biopsy specimens of 19 control subjects with normogastrinemia and 6 patients with hypergastrinemia. In controls, the HDC activity was 3 fold higher in fundic mucosa (120 +/- 13 fmol/min/mg protein, mean +/- S.E.) than in a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90424-a
更新日期：1991-01-01 00:00:00
abstract：:The leukotriene (LT) synthesis inhibitors BAY x1005 and MK-886 were evaluated in human lung parenchyma challenged with an anti-IgE. The anti-IgE-induced LTE4 release was time- and dose-dependent. Treatment of the parenchyma with indomethacin (3 microM) prior to anti-IgE challenge inhibited the 6-keto prostaglandin F1 ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00426-2
更新日期：1996-01-01 00:00:00
abstract：:High concentrations of cocaethylene (EC), the ethyl ester of benzoylecgonine, were measured in the blood of individuals who had concurrently used cocaine and ethanol. Since the powerful reinforcing effects of cocaine appear to be dependent on inhibition of dopamine reuptake in brain, we compared the effects of EC on t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90217-y
更新日期：1991-01-01 00:00:00
abstract：UNLABELLED:The mechanisms by which exercise affects atherosclerotic plaque stability remain incompletely understood. We evaluated the effects of two training protocols on both atherosclerotic plaque structure and the signaling pathways involved in plaque rupture. METHODS:Male low-density lipoprotein (LDL) receptor kno...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2016.04.007
更新日期：2016-05-15 00:00:00
abstract：:The patterns of prolactin release after bilateral ECT and after 0.4 mg TRH i.v. were studied in 11 female melancholic patients in a 5-min sampling protocol. Mean prolactin peaking times were 10.2 min after ECT and 20.5 min after TRH. The elimination rate coefficients were significantly lower--and the corresponding hal...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90206-2
更新日期：1987-09-07 00:00:00
abstract：:The effect of exosomes on receptor cells participating in intercellular communication has been extensively studied, but the effect of exosomes on donor cells remains unclear. It has been reported that exosomes secreted by renal proximal tubular epithelial cells (PTECs) under different stimuli accelerate acute and chro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118347
更新日期：2020-11-15 00:00:00
abstract：:The anticonvulsant activity of U-54494A was studied in a 4-aminopyridine (4-AP) epilepsy model using extracellular recordings in in vitro hippocampal slices. Field potentials were evoked by stimulation of Schaffer collaterals, and recorded from the CA1 region of the hippocampus after infusion of 4-AP in the absence an...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90381-x
更新日期：1992-01-01 00:00:00
