Abstract:
:This in vitro study was conducted to determine whether tachyphylaxis of guinea pig airway to furosemide occurs under conditions that produce tachyphylaxis to the beta 2-adrenoceptor agonist, salbutamol. Isometric tension was measured in tracheal rings bathed in HEPES buffer from 4-6 d newborn guinea pigs of either sex, and 6 wk old males. Paired rings were first incubated with furosemide, 30 or 300 microM, or control for 60 min, washed, then constricted with 3 microM acetylcholine. At stable contraction, relaxation to furosemide (30 microM-1 mM) was measured. For comparison, similar experiments were performed with 10 microM salbutamol incubation for 30 min. 86Rb uptake, a marker for K+ transport and Na-K-Cl cotransport activity, was also measured in these airway segments. Pre-exposure to these airway relaxants did not affect contractile force generation by acetylcholine. Tracheal desensitization to both salbutamol and furosemide was observed. Partial recovery of furosemide induced relaxation was seen one hour after desensitization. Pre-exposure to 300 microM furosemide did not inhibit the decrease in 86Rb uptake normally observed with furosemide. In summary, we found that: 1) tachyphylaxis of guinea pig airway relaxation occurred with both salbutamol and furosemide under similar experimental conditions; however 2) inhibition of 86Rb uptake by furosemide was not affected by prior exposure. Taken together, these results suggest that furosemide induced airway relaxation could be affected by repeated or prolonged exposure, but this response may not be associated with changes in furosemide-sensitive Na-K-Cl cotransporter activity.
journal_name
Life Scijournal_title
Life sciencesauthors
Iwamoto LM,Wilson VL,Lavallee SL,Fujiwara N,Ayau EL,Nakamura KTdoi
10.1016/0024-3205(96)00416-xsubject
Has Abstractpub_date
1996-01-01 00:00:00pages
1015-24issue
13eissn
0024-3205issn
1879-0631pii
002432059600416Xjournal_volume
59pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract:AIMS:Mancozeb is a dithiocarbamate fungicide known to be genotoxic and induces tumors in rodents at various sites. There is no report in the literature about its genotoxicity in humans. Here, we investigated the association between mancozeb exposure and induction of genotoxic and proapoptotic changes in cultured human ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2011.12.013
更新日期:2012-06-06 00:00:00
abstract::In an in vitro bioassay system for adrenocorticotropic hormone using isolated rat adrenal cells, kaurenol, a diterpene alcohol, stimulated corticosterone production and augmented the steroidogenic effect of adrenocorticotropin or forskolin, dose-dependently. Kaurenol had no effect on cyclic AMP production by the cells...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90070-6
更新日期:1986-02-03 00:00:00
abstract::To determine how protein kinase C (PKC) activity influences properties of the tetrodotoxin-resistant sodium current (TTX-R I(Na)) in neonatal rat nodose ganglion (NG) neurons, we assessed the effects of phorbol,-12-myristate,13-acetate (PMA), one of the PKC activators, and staurosporine, one of the PKC inhibitors, on ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.04.053
更新日期:2005-11-19 00:00:00
abstract::Diltiazem, diazepam, and clonazepam effectively inhibit Na+-induced Ca2+ release from mitochondria isolated from rabbit heart and rat brain. At 10 mM NaCl, the 50% inhibition of the rate of Ca2+ release occurred at 5, 7, 40 and 350 microM of clonazepam d-cis-diltiazem, diazepam and 1-cis-diltiazem, respectively. Ro 15...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90319-3
更新日期:1983-06-20 00:00:00
abstract:AIMS:In pulmonary arterial hypertension (PAH), increases in endothelin-1 (ET-1) contribute to elevated pulmonary vascular resistance which ultimately causes death by right ventricular (RV) heart failure. ET antagonists are effective in treating PAH but lack efficacy in treating left ventricular (LV) heart failure, wher...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.02.020
更新日期:2014-11-24 00:00:00
abstract::Pretreatment with a low dose of 3-nitropropionic acid (3-NPA) has been shown to induce ischemic tolerance in the gerbil hippocampus. It is well known that sublethal (2-min) ischemia also induces ischemic tolerance. To investigate the acquisition of ischemic tolerance with 3-NPA, we examined the protein expression afte...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.01.037
更新日期:2005-10-21 00:00:00
abstract::T cell receptor (TCR/CD3) induced fluctuations in intracellular free ionizied calcium, [Ca2+]i, was analysed in the human T leukemia cell clone, Jurkat, cultured in the presence of the opioid methionine enkephalinamide (Met-Enk) in titrated concentrations (10[-7] to 10[-15] M) or saline (PBS). In the majority of indiv...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00055-1
更新日期:1998-01-01 00:00:00
abstract::An improved two-dimensional HPTLC procedure was developed for separating phospholipids including individual phosphoinositides, phosphatidic acids and plasmalogens. This procedure was used to examine the time course for uptake of label by phospholipids in brain subcellular membranes after intracerebral injection of [ga...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90475-x
更新日期:1989-01-01 00:00:00
abstract::This paper describes a new formulation for triggered delivery of insulin which consisted of magnetic particles dispersed in alginate spheres. When magnetic field was applied to that system, the release rate of insulin was about 50 times higher than in the absence of magnetic field. The influence of oscillating magneti...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90009-4
更新日期:1988-01-01 00:00:00
abstract::Uptake of 131iodine-metaiodobenzylguanidine (131I-MIBG) by 6-23 rat medullary thyroid carcinoma (MTC), was studied in vitro and in vivo. In vitro, there was an 8-fold increase in 131I uptake by 6-23 cells when labeled with 131I-MIBG (131I 24 +/- 15 cpm/10(6) cells, 131I-MIBG 196 +/- 9 cpm/10(6) cells). MIBG uptake in ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90456-6
更新日期:1989-01-01 00:00:00
abstract::Insulin binding and 3-0-Methylglucose transport have been studied in erythrocyte- and reticulocyte-enriched fractions of blood cells in order to determine if the increased number of insulin binding sites in reticulocytes is associated with a glucose transport response to insulin. In these experiments rabbit reticulocy...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90588-4
更新日期:1982-07-19 00:00:00
abstract::This study was designed to document possible changes in bupivacaine (B) local anaesthetic activity and pharmacokinetics in mice after a ketamine (K) injection. In the experiments, bupivacaine (8.25 mg.kg(-1)), was injected into the popliteal space of the right posterior limb: the local anaesthetic activity was assesse...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00861-8
更新日期:1997-01-01 00:00:00
abstract::This study examines the effects of prenatal cocaine administration on the development of vascular sympathetic innervation and contractile responsiveness. Rabbits received cocaine (4 mg/kg, iv, bid) or saline during gestational days 8 to 29. Aortas were obtained on postnatal days 10, 20, 30 and 50. Vascular smooth musc...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00765-9
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Most hepatocellular carcinoma cases are diagnosed at late stages of the disease, which makes it the second cause of cancer mortality worldwide. For advanced-stage patients, chemotherapeutic drugs are the best treatment option; however, their adverse effects and high cost are still major obstacles for effecti...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118827
更新日期:2021-01-15 00:00:00
abstract::Since reduced levels of brain serotonin are known to cause behavioural abnormalities, to which diabetics are also prone, we investigated the effect, in rats, of chronic diabetes on brain serotonin concentration and on the numbers of 5-HT(1A) and 5-HT2 receptors in cerebral cortex and brainstem. Our data show that stre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00084-2
更新日期:1997-01-01 00:00:00
abstract::A simple and sensitive method using high performance liquid chromatography with fluorometric detection has been developed for the identification and quantitation of the endogeneous tryptamine metabolites, indoleacetic acid (IAA) and tryptophol (TOL) in the normal mouse brain. The limits of sensitivity are 5pg for both...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90744-0
更新日期:1983-11-14 00:00:00
abstract::A new simplified procedure for determination of nitric oxide (NO) in biological solutions is described utilizing a new reducing system of nitric oxide prior to chemiluminescence. Advantages of the new method makes heating of the reducing solution unnecessary and avoids cooling and condensation of generated vapors. Onl...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90625-y
更新日期:1992-01-01 00:00:00
abstract::Extracellular phospholipase A2 (PLA2) with proinflammatory activity has recently been discovered in synovial fluids in inflammatory arthritides. In the search for the sources of synovial fluid PLA2, human synovium and articular cartilage were found to contain large quantities of the enzyme. In rheumatoid arthritis (RA...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90381-k
更新日期:1991-01-01 00:00:00
abstract:AIMS:Extensive evidence supports a role for voltage-gated calcium channels (VGCC) and TRPV1 receptors in pain transmission and modulation. We investigated the profile of analgesic interaction between Phα1β toxin (a VGCC blocker) and SB366791 (selective TRPV1 antagonist) in a model of acute pain induced by capsaicin. Ch...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.06.018
更新日期:2017-08-01 00:00:00
abstract::To analyze the metabolisms of platelet-activating factor (PAF) and Thromboxane A2 (TxA2) when platelets and mesangial cells (MC) interact, immunoreactive thromboxane B2 (TxB2) and PAF were measured after incubation of cultured rat MC with platelets (P) and with platelet supernatants (PS). In both cases, TxB2 significa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02030-m
更新日期:1995-01-01 00:00:00
abstract:AIMS:The purpose of this study was to determine a relatively short-term effect of feeding an α-linolenic acid (ALA, n-3)-restricted, linoleic acid (LA, n-6)-adequate diet on neurotrophin contents and protein kinase activities in brain regions of the mouse. MAIN METHODS:After feeding mice a safflower oil (SAF) diet (AL...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.09.003
更新日期:2010-10-09 00:00:00
abstract:AIMS:The mechanism of cough hypersensitivity induced by particulate matter 2.5 (PM2.5) remains elusive. The current study was designed to explore the effect of transient receptor potential vanilloid-1 (TRPV1) on cough hypersensitivity in airway and central nervous system. MAIN METHODS:The PM2.5-induced chronic cough m...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.02.064
更新日期:2016-04-15 00:00:00
abstract::Inflammation, inflammatory mediators, cyclooxygenase (COX)-2, and inducible nitric oxide (iNOS) are all influenced by age-related oxidative status. To investigate the effect of dietary fish oil (FO) and calorie restriction (CR) on oxidative stress-related inflammatory status with age, (NZB/NZW) F1 (B/W) mice were fed ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.10.034
更新日期:2006-04-18 00:00:00
abstract::The temperature effects of naloxone and naltrexone (1-30 mg/kg) were examined in well habituated male rats. These drugs had a similar time course and potency, producing a dose-dependent hypothermia followed several hours later by a hyperthermia. A subsequent study found that not only did 30 mg/kg of naloxone or naltre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90563-7
更新日期:1987-03-16 00:00:00
abstract:AIM:A variety of evidence suggests that vitamin D can prevent the development of multiple sclerosis (MS). TGF-β pathway genes also play important roles in MS. Here, we aim to study whether vitamin D affects TGF-β pathway gene expression and Expanded Disability Status Scale (EDSS) scores in MS patients. MAIN METHODS:A ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.05.017
更新日期:2015-08-01 00:00:00
abstract::In an effort to obtain highly potent glucagon antagonists, we have investigated glucagon (1) structure-function relationships utilizing the following design principles: (1) structural changes known to lead to partial agonist activities; (2) conformational restrictions; (3) changes in the conformational probabilities o...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90513-3
更新日期:1993-01-01 00:00:00
abstract::Opiate binding sites have been characterized on membranes from bovine adrenal medullas and six human pheochromocytomas. In human tumors, large variations in site distribution were observed. Kappa and benzomorphan sites represented the majority of the sites detected. The heterogeneity of the opiate sites on these tissu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90501-5
更新日期:1983-01-01 00:00:00
abstract::A highly purified rat urinary bladder smooth muscle cell culture was obtained by a modified enzymic isolation method, and the presence of functional muscarinic as well as beta-adrenergic receptors were subsequently determined. At 7-10 days of culture, cells became elongated and spindle-shaped showing a typical "hills ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01488-6
更新日期:2002-01-25 00:00:00
abstract::TCDD was assessed as a biological response modifier for increasing MMC cytotoxicity through aryl hydrocarbon receptor (AhR) activation and increasing levels of bioreductive enzymes. Human MCF-7 cells were exposed to TCDD, MMC and combinations thereof under aerobic or hypoxic conditions. Cytotoxicity, enzyme activities...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.07.021
更新日期:2006-02-23 00:00:00
abstract::We produced thiamine-deficient (TD) mice by TD diet treatment. The growth curve of mice on TD feeding was sharply increased until on the 10th day and subsequently the body weight gradually decreased. The mortality rate in mice was about 67% on the 30th day after the start of TD feeding. We performed the forced swimmin...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01206-1
更新日期:2001-07-27 00:00:00