Thromboxane A2 and platelet-activating factor decrease in the platelet-mesangial cell interactions.


:To analyze the metabolisms of platelet-activating factor (PAF) and Thromboxane A2 (TxA2) when platelets and mesangial cells (MC) interact, immunoreactive thromboxane B2 (TxB2) and PAF were measured after incubation of cultured rat MC with platelets (P) and with platelet supernatants (PS). In both cases, TxB2 significantly decreased with respect to the P synthesis and to the PS content, suggesting an increased degradation of this metabolite or even the existence of a specific effect of MC upon platelet TxB2. When immunoreactive PAF was measured, results were comparable to those observed for TxB2. Moreover, when intrinsic mesangial cell synthesis of PAF was assessed by analyzing the [3H]-acetate incorporation by prelabeled MC in the HPLC fraction coeluting with cold PAF standards, it was possible to demonstrate that P or PS did not modify PAF synthesis in these cells. In summary, present results support the existence of a specific effect of mesangial cells upon platelet TxA2 and PAF.


Life Sci


Life sciences


Arribas I,Rodríguez-Puyol D,García-Escribano MC,Díez-Marques ML,Pérez de Lema G,Rodríguez-Puyol M




Has Abstract


1995-01-01 00:00:00














  • A non-radioactive fluorescent method for measuring protein kinase C activity.

    abstract::The purpose of this study is to report the development of a non-radioactive fluorescent peptide assay for measuring protein kinase C activity (PKC). The assay is based on a glycogen synthase derived fluorescent peptide that is phosphorylated by PKC. Phosphorylation causes the peptide to migrate toward the anode while ...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Isbell JC,Christian ST,Mashburn NA,Bell PD

    更新日期:1995-01-01 00:00:00

  • Metabolic profiling of radioreceptor-assayable opioid peptides in a human pituitary ACTH-secreting tumor.

    abstract::The profile of endogenous opioid peptides in the peptide-rich fraction obtained from a homogenate of an ACTH-secreting human pituitary tumor is presented. Gradient RP-HPLC is used to separate the mixture into peptide constituents. A preparation of opioid receptors is used in a radioreceptor assay with ethorphine - a r...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Desiderio DM,Cezayirli RC,Fridland G,Robertson JT,Sacks H

    更新日期:1985-11-11 00:00:00

  • Inhibition of Sendai virus by various snake venom.

    abstract::Viperid, elapid and crotalid snake venoms were screened in vitro for antiviral activity against Sendai virus. The hemolysis of 10(8) human erythrocytes in 1 ml, caused by 70 HAU of Sendai virus, was abolished when the virions were pretreated with 10 ug of the viperid venom of Echis coloratus, and was considerably dimi...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Borkow G,Ovadia M

    更新日期:1992-01-01 00:00:00

  • MicroRNA-1 inhibits proliferation of hepatocarcinoma cells by targeting endothelin-1.

    abstract:AIMS:MicroRNA-1 (miR-1) has been demonstrated as a tumor-suppressive miRNA, which shows a down-regulated pattern in several human malignancies including hepatocellular carcinoma (HCC). However, the pathophysiologic roles of miR-1 and their mechanisms in HCC tumorigenesis are still not totally elucidated. MAIN METHODS:...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Li D,Yang P,Li H,Cheng P,Zhang L,Wei D,Su X,Peng J,Gao H,Tan Y,Zhao Z,Li Y,Qi Z,Rui Y,Zhang T

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    journal_title:Life sciences

    pub_type: 杂志文章,收录出版


    authors: Li Y,Qin HQ,Chen QS,Wang JJ

    更新日期:2005-01-28 00:00:00

  • Azaftig, a urinary proteoglycan from a cachectic cancer patient, causes profound weight loss in mice.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Figueroa J,Vijayagopal P,Debata C,Prasad A,Prasad C

    更新日期:1999-01-01 00:00:00

  • Environmental crowding modifies responding to noxious stimuli and the effects of mu- and kappa-agonists.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Pilcher CW,Browne J

    更新日期:1982-09-20 00:00:00

  • Dextrorphan: an antagonist for phencyclidine receptors.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Sun FY,Zhu H,Zhang LM,Xu XR,Zhang AZ

    更新日期:1987-06-15 00:00:00

  • Methotrexate stimulation of asparagine synthetase activity in rat liver.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Maul DM,Schuster SM

    更新日期:1982-03-22 00:00:00

  • Increased endogenous nitric oxide production induced by physical exercise in peripheral arterial occlusive disease patients.

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    journal_title:Life sciences

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Arosio E,Cuzzolin L,De Marchi S,Minuz P,Degan M,Crivellente F,Zannoni M,Benoni G

    更新日期:1999-01-01 00:00:00

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    journal_title:Life sciences

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    authors: Zhu D,Li R,Liu G,Hua W

    更新日期:1999-01-01 00:00:00

  • The negative effect of dexamethasone on calcium-processing gene expressions is associated with a glucocorticoid-induced calcium-absorbing disorder.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Kim MH,Lee GS,Jung EM,Choi KC,Jeung EB

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  • Platelet-activating factor stimulates production of prostaglandin E2 in murine osteoblast-like cell line MC3T3-E1.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Shibata Y,Ogura N,Moriya Y,Abiko Y,Izumi H,Takiguchi H

    更新日期:1991-01-01 00:00:00

  • Calcium, microfilaments and morphogenesis.

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    journal_title:Life sciences

    pub_type: 杂志文章,评审


    authors: Stanisstreet M,Jumah H

    更新日期:1983-10-10 00:00:00

  • alpha-MSH analogues and adrenal zona glomerulosa function.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Vinson GP,Whitehouse BJ,Bateman A,Hruby VJ,Sawyer TK,Darman PS

    更新日期:1984-08-06 00:00:00

  • Effect of 12, 24 and 72 hours fasting in thermogenic parameters of rat brown adipose tissue mitochondrial subpopulations.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Gianotti M,Clapés J,Lladó I,Palou A

    更新日期:1998-01-01 00:00:00

  • In vitro comparative analysis of human dental stem cells from a single donor and its neuronal differentiation potential evaluated by electrophysiology.

    abstract:AIMS:The aim of this study was to find out a mesenchymal stem cells (MSCs) source from human dental tissues of the same donor (follicle, papilla and pulp), which exhibits higher neurogenic differentiation potential in vitro. MAIN METHODS:MSCs were isolated from dental tissues (follicle, papilla and pulp) by digestion ...

    journal_title:Life sciences

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    authors: Ullah I,Subbarao RB,Kim EJ,Bharti D,Jang SJ,Park JS,Shivakumar SB,Lee SL,Kang D,Byun JH,Park BW,Rho GJ

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  • Tet3 enhances IL-6 expression through up-regulation of 5-hmC in IL-6 promoter in chronic hypoxia induced atherosclerosis in offspring rats.

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    journal_title:Life sciences

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    authors: Zhang P,Chen X,Zhang Y,Su H,Zhang Y,Zhou X,Sun M,Li L,Xu Z

    更新日期:2019-09-01 00:00:00

  • Insulin-like growth factor-1 is not mitogenic for the Walker-256 carcinosarcoma.

    abstract::This study was designed to determine whether intravenous infusion of recombinant human insulin-like growth factor 1 (IGF-1) stimulates tumor growth. In order to determine the potential interaction between nutrition and IGF-1 administration the study was conducted in fasting rats and during continuous feeding by total ...

    journal_title:Life sciences

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    authors: Wan JM,Istfan NW,Ye SL,Bistrian BR

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  • Alterations of plasma opioid activity in human diabetics.

    abstract::Plasma opioid levels were determined in 9 obese non-diabetic subjects, their 8 age matched controls, and in 29 diabetic patients; 10 maintained on diet alone, 6 on an oral hypoglycemic agent (chlorpropamide) and 13 treated with insulin. Five age matched controls for the diabetic groups were also studied for comparison...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Awoke S,Voyles NR,Bhathena SJ,Tanenberg RJ,Recant L

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  • Time course sequences of angiotensin converting enzyme and chymase-like activities during development of right ventricular hypertrophy induced by pulmonary artery constriction in dogs.

    abstract::ACE and chymase play crucial roles in the establishment of pressure overload-induced cardiac hypertrophy. In the present study, time sequences of ACE and chymase-like activities, and their correlation with hypertrophic changes including free wall thickness and cardiac fibrosis, were elucidated in dogs with constant pr...

    journal_title:Life sciences

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    authors: Orito K,Yamane T,Kanai T,Fujii Y,Wakao Y,Matsuda H

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  • GABAergic neurons in the insular cortex play an important role in cue-morphine reward memory reconsolidation.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Sun K,Xiao L,Wu Y,Zuo D,Zhang C,Liu S,He Z,Rong S,Wang F,Sun T

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  • Dramatic limbic and cortical effects mediated by high affinity PCP receptors.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Piercey MF,Ray CA

    更新日期:1988-01-01 00:00:00

  • Effects of prenatal administration of testosterone and cortisone on the reproductive system of the female rat.

    abstract::Testosterone propionate, cortisone, or sesame oil vehicle were given to rats during the last week of pregnancy so that effects of the hormones on anogenital distance, breeding capacity and vaginal opening of the female progeny could be contrasted. Testosterone significantly increased anogenital distance and delayed va...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: McCoy SJ,Shirley BA

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Millis RM,Austin RE,Bond V,Faruque M,Goring KL,Hickey BM,Blakely R,Demeersman RE

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Mereu G,Corda MG,Carcangiu P,Giorgi O,Biggio G

    更新日期:1987-04-06 00:00:00

  • Protein phosphorylation in isolated human adipocytes-adrenergic control of the phosphorylation of hormone-sensitive lipase.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Smiley RM,Paul S,Browning MD,Leibel RL,Hirsch J

    更新日期:1990-01-01 00:00:00

  • Induction of mitotic arrest and apoptosis by evodiamine in human leukemic T-lymphocytes.

    abstract::Leukemias are a heterogenous group of diseases characterized by uncontrolled proliferation of abnormal blood cells of hematopoietic system. Evodiamine, a characteristic alkaloid extracted from Evodia fruits, has been reported to exhibit inhibitory effect on cell proliferation and migration in several types of cancer c...

    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Huang YC,Guh JH,Teng CM

    更新日期:2004-05-21 00:00:00

  • Anterior gradient 2-derived peptide upregulates major histocompatibility complex class I-related chains A/B in hepatocellular carcinoma cells.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Bian J,He L,Wu Y,Liu W,Ma H,Sun M,Yu J,Yu Z,Wei M

    更新日期:2020-04-01 00:00:00

  • Cholecystokinin receptor mediated hydrolysis of inositol phospholipids in guinea pig gastric glands.

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    journal_title:Life sciences

    pub_type: 杂志文章


    authors: Chang RS,Lotti VJ,Chen TB

    更新日期:1985-03-11 00:00:00