Abstract:
:In the present study we compared, in vivo in rats, the hepatobiliary transport of monovalent (silver:Ag) and divalent metals (zinc:Zn; cadmium:Cd) with that of copper (Cu). Cu can have two oxidation states in vivo, i.e. Cu(I) and Cu(II). Studies were performed in normal Wistar (NW) rats and mutant GY Wistar rats. The latter express defective canalicular ATP-dependent glutathione-conjugate transport (cMOAT); reduced glutathione (GSH) is virtually absent in bile of these mutants. Cd (400 nmol/100g body wt, i.v.) was rapidly secreted into bile in NW rats concommitant with a 4-fold increase in biliary GSH secretion. In contrast, biliary Cd concentrations remained below detection limits in GY rats. Injection of Zn (1500 nmol/100g body wt) did not affect Zn secretion in GY rats and resulted only in a very small increase in NW rats (recovery < 2%). The biliary secretion pattern of Ag (800 nmol/100g body wt, i.v.) was highly similar to that of Cu (260 nmol/100g body wt). A biphasic pattern composed of a rapid and slow phase was observed in NW rats for both metals with a recovery of 48.5 +/- 10.6% and 44.9 +/- 8.4% of the dose for Ag and Cu, respectively. In GY rats, the rapid phase of both Ag and Cu secretion was absent and recoveries were 23.2 +/- 3.6% and 19.7 +/- 3.2%, respectively. When Ag and Cu were administered simultaneously, the recoveries of Ag and Cu were decreased in NW and GY rats when compared to single administration. Our data indicate that divalent and monovalent metals are secreted into bile via different transport systems in the rat. The absence of Cd and Zn secretion into bile of GY rats after their i.v. administration suggest a role of cMOAT in their biliary elimination. Cu and Ag probably share common transport systems for hepatobiliary removal, being in part dependent on the presence of either GSH in bile or cMOAT activity or on both. The GSH-independent portion of transport, i.e. the slow phase, may be mediated by the newly identified Cu transporting P-type ATPase (cCOP).
journal_name
Life Scijournal_title
Life sciencesauthors
Dijkstra M,Havinga R,Vonk RJ,Kuipers Fdoi
10.1016/0024-3205(96)00447-xsubject
Has Abstractpub_date
1996-01-01 00:00:00pages
1237-46issue
15eissn
0024-3205issn
1879-0631pii
002432059600447Xjournal_volume
59pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::The analgesic effects of bestatin and thiorphan, two enzymatic inhibitors protecting endogenous enkephalins from their degradation, and those of DAGO and deltakephalin, respectively mu and delta opioid peptides, are assessed on the electrical stimulation test of the mouse tail. The relative analgesic potency of DAGO a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90595-7
更新日期:1983-01-01 00:00:00
abstract::Accumulation of excess fluoride in the environment poses serious health risks to plants, animals, and humans. This endangers human health, affects organism growth and development, and negatively impacts the food chain, thereby affecting ecological balance. In recent years, numerous studies focused on the molecular mec...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2018.02.001
更新日期:2018-04-01 00:00:00
abstract:OBJECTIVE:This study investigated the role of long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) in kidney injury induced by urine-derived sepsis (US). MATERIALS AND METHODS:An Escherichia coli suspension was injected into the distal ureter of adult male Sprague Dawley rats to establish a US model. Lipo...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.08.041
更新日期:2018-10-01 00:00:00
abstract::Leptin-deficient Ob/Ob mice are hypometabolic and have reduced fat cell expression of beta-3 adrenoceptors (ARs). To determine whether leptin repletion restores beta-3 AR number, C57BL/6J Ob/Ob mice were given exogenous leptin (5 mg/kg I.P. daily) for 21 days. Leptin administration reduced body weight from 43.1+/-3.7 ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00358-5
更新日期:1997-01-01 00:00:00
abstract::1-oleoyl-2-acetyl glycerol (OAG), a potent activator of protein kinase C, inhibited the binding of 125I-labelled epidermal growth factor (EGF) in isolated rat pancreatic acini. Unlike cholecystokinin-octapeptide (CCK8) and the C-kinase activator 12-O-tetradecanoyl phorbol-13-acetate (TPA), two inhibitors of 125I-EGF e...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90129-9
更新日期:1987-04-20 00:00:00
abstract::Glycated human serum albumin (glc HSA) and glc proteins in back hairs from diabetic rats were deglycated by incubation with hydrazine or aminoguanidine (guanylhydrazine) at physiological temperature (37 degrees C). Glc HSA decreased by 35% with 0.2 M hydrazine at day 20 and by 56% with 0.1 M aminoguanidine at day 10. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90681-r
更新日期:1993-01-01 00:00:00
abstract::The effect of adrenergic agents on protein phosphorylation in human adipocytes was examined. Freshly isolated human fat cells were incubated with 32PO4 in order to label intracellular ATP, then treated with a variety of adrenergic and other pharmacologic agents. Treatment with the beta-adrenergic agonist isoproterenol...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90597-k
更新日期:1990-01-01 00:00:00
abstract:AIM:We previously reported the possible involvement of mucosal nitric oxide (NO)-triggered 5-HT release in luminal glutamate sensing by the gastric vagus nerve, and we proposed that the stomach, like the duodenum, could "taste" luminal nutrients. Nitric oxide synthase (NOS) is widely distributed in the gastric mucosa, ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.10.009
更新日期:2009-12-16 00:00:00
abstract::Since reduced levels of brain serotonin are known to cause behavioural abnormalities, to which diabetics are also prone, we investigated the effect, in rats, of chronic diabetes on brain serotonin concentration and on the numbers of 5-HT(1A) and 5-HT2 receptors in cerebral cortex and brainstem. Our data show that stre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00084-2
更新日期:1997-01-01 00:00:00
abstract:AIM:Although constitutive autophagy is linked to redox state and participates in cell homeostasis, it is scarcely known if redox state, autophagy, and lysosomal function depend on sex, a factor that largely influences health and diseases. Therefore, we evaluated the existence of sex differences in redox state and const...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.06.001
更新日期:2013-08-06 00:00:00
abstract::The effect of maternal glucocorticoid depletion upon the fetal development of the organ of Zuckerkandl (OZ) in rats was determined. Maternal hypophysectomy at 13d8h gestation resulted in a fifty percent decrease in plasma corticosterone levels at 18d8h when compared to both sham operated and unoperated controls. No di...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90609-6
更新日期:1987-09-14 00:00:00
abstract::Adenylate cyclase activity in pancreatic acinar cell membranes was determined in rats that had undergone a treatment with pentagastrin (250 micrograms/kg, intraperitoneal three times daily) for 1 week or that had undergone small bowel resection (90%) and were sacrified at 2 weeks, 1 month and 6 months after interventi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)02226-9
更新日期:1995-01-01 00:00:00
abstract::Excessive fibrosis and extracellular matrix deposition resulting from upregulation of target genes expression mediated by transforming growth factor-beta (TGF-β)/SMAD and hypoxia inducible factor-1 (HIF-1) signaling pathways are the main mechanisms that drive keloid formation. Sumoylation is a protein posttranslationa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117859
更新日期:2020-08-15 00:00:00
abstract::CYP3A4, the predominant cytochrome P450 (CYP) expressed in human liver, contributes to the metabolism of approximately half the drugs in use today. In general, human-derived cell lines fail to express CYPs. It was previously shown that CYP3A4 mRNA and CYP3A immunoreactive protein are induced by 1alpha,25-dyhydroxyvita...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00262-5
更新日期:2003-05-30 00:00:00
abstract:AIMS:The purpose of this study was to determine a relatively short-term effect of feeding an α-linolenic acid (ALA, n-3)-restricted, linoleic acid (LA, n-6)-adequate diet on neurotrophin contents and protein kinase activities in brain regions of the mouse. MAIN METHODS:After feeding mice a safflower oil (SAF) diet (AL...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.09.003
更新日期:2010-10-09 00:00:00
abstract::2',6'-Dimethyl substitution of the Tyr(1) residue of opioid agonist peptides and deletion of the positively charged N-terminal amino group or its replacement with a methyl group has recently been shown to represent a general structural modification to convert opioid peptide agonists into antagonists. This conversion r...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00389-8
更新日期:2003-06-27 00:00:00
abstract::Mitochondria are known to develop a series of abnormalities as a result of ischemia. The inability of mitochondria to resume normal function following reperfusion has been implicated as an important factor in irreversible cell damage. However, the mechanism of mitochondrial injury after ischemic brain insult is poorly...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90627-7
更新日期:1985-07-08 00:00:00
abstract::To study the role of IL-5 in allergic airway hyperreactivity, the time course for the production of cytokines, the infiltration of inflammatory cells and the onset of airway hyperreactivity after three inhalations of antigens were studied in mice. The effect of the soluble alpha-chain of murine recombinant interleukin...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)90139-2
更新日期:1994-01-01 00:00:00
abstract::Abnormal activation of the beta-catenin signaling pathway can cause various types of cancer. Activation of Wnt pathway leads to stabilization of the beta-catenin protein, which results in its translocation to the nucleus and the formation of complexes with lymphoid enhancer factor-1 (LEF-1) and other T-cell factor (TC...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.02.009
更新日期:2005-07-29 00:00:00
abstract::Peptides derived from tryptic hydrolysate of jumbo squid (Dosidicus gigas) skin gelatin were assessed for their antioxidant properties in different in vitro assay systems. The hydrolysate itself exhibited a strong lipid peroxidation inhibition and it was much higher than that of natural antioxidant, alpha-tocopherol. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.03.016
更新日期:2005-09-09 00:00:00
abstract::Since Oct-1 is a ubiquitous DNA binding protein shown to play an important role in regulating cell proliferation and possess structural characteristics consistent with a nitric oxide (NO) target, we studied NO regulation of the DNA binding activity of Oct-1 in the A7R5 vascular smooth muscle cell (VSMC) line. Two NO d...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)01172-7
更新日期:1998-01-01 00:00:00
abstract::This study was essentially an in vivo protection experiment designed to test further the hypothesis that stress induces release of endogenous opioids which then act at opioid receptors. Rats that were either subjected to restraint stress for 1 hr or unstressed were injected ICV with either saline or 2.5 micrograms of ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90430-9
更新日期:1987-12-28 00:00:00
abstract::Endothelin-1 (ET-1) has been proposed as one of the possible mediators of the vasoconstriction seen following ischemia and reperfusion. We investigated the effect of ischemia and reperfusion on the contractile response of canine renal and iliac arteries to the dihydropyridine-type calcium channel agonist (+/-)Bay K 86...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00267-a
更新日期:1995-01-01 00:00:00
abstract:AIMS:In pulmonary arterial hypertension (PAH), increases in endothelin-1 (ET-1) contribute to elevated pulmonary vascular resistance which ultimately causes death by right ventricular (RV) heart failure. ET antagonists are effective in treating PAH but lack efficacy in treating left ventricular (LV) heart failure, wher...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.02.020
更新日期:2014-11-24 00:00:00
abstract::The immunosuppressive effects of microcolin A, a lipopeptide extracted from the marine blue green alga Lyngbya majuscula were investigated. Microcolin A suppressed concanavalin A (IC50 = 5.8 nM), phytohemagglutinin (IC50 = 12.5 nM) and lipopolysaccharide (IC50 = 8.0 nM) induced proliferation of murine splenocytes. Mix...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(96)00645-5
更新日期:1997-01-01 00:00:00
abstract::Aim Deformity in the cellular homeostatic event associated with cell survival and apoptosis are committing factors for carcinogenesis. Interventions of these events by pharmacologically active agent gain predominance in cancer treatment. In current investigation Solanidine, a steroidal alkaloid was evaluated on tumori...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.03.015
更新日期:2018-04-15 00:00:00
abstract::Identification of the neural substrates subserving audiogenic convulsions in the GEPR is an important task and while it is not yet complete, many laboratories employing various techniques have contributed importantly to our current understanding. The present review focuses on the use of lesions to identify the neural ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90367-x
更新日期:1986-09-08 00:00:00
abstract::Three cholecystokinin type B (CCKB) receptor antagonists were labelled with 11C and evaluated ex vivo in rat biodistribution studies. The CCKB antagonists were YF 476 and two other compounds of the basic 3-ureido-1,4-benzodiazepine class. Following tail-vein administration of [11C]-YF 476 exceedingly low levels of rad...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)01021-3
更新日期:2001-02-02 00:00:00
abstract::The potency of six antimuscarinic oxotremorine analogs at sympathetic ganglionic M1 and brainstem M2 muscarinic receptors was compared in the rat. Inhibition of the pressor effects of McNA343 or physostigmine was used as functional indicators of M1 and M2 blockade, respectively. 50% inhibitory doses (ID50) were calcul...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90442-t
更新日期:1990-01-01 00:00:00
abstract:AIMS:LexA protein is a transcriptional repressor which regulates the expression of more than 60 genes belonging to the SOS global regulatory network activated by damages to bacterial DNA. Considering its role in bacteria, LexA represents a key target to counteract bacterial resistance: the possibility to modulate SOS r...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.117116
更新日期:2020-01-15 00:00:00