Abstract:
:A new simplified procedure for determination of nitric oxide (NO) in biological solutions is described utilizing a new reducing system of nitric oxide prior to chemiluminescence. Advantages of the new method makes heating of the reducing solution unnecessary and avoids cooling and condensation of generated vapors. Only traces of acid with a high boiling point are used. The method permits analysis of small sample volumes (200 microL). The basal production of nitric oxide by freshly harvested endothelial cells ranged from 100 to 880 picomoles.
journal_name
Life Scijournal_title
Life sciencesauthors
Termin A,Hoffmann M,Bing RJdoi
10.1016/0024-3205(92)90625-ysubject
Has Abstractpub_date
1992-01-01 00:00:00pages
1621-9issue
20eissn
0024-3205issn
1879-0631pii
0024-3205(92)90625-Yjournal_volume
51pub_type
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