Abstract：
:The best conditions for extractions of free pyrroloquinoline quinone (PQQ) from crude biological samples were investigated with various organic solvents and Sep-Pak C18 cartridges. PQQ was measured with use of its native fluorescence in aqueous solution. PQQ was well extracted into n-butanol under acid conditions, and addition of NaCl did not improve the solvent extraction. PQQ, which had been extracted into n-butanol, could be re-extracted into an aqueous phase by addition of either n-heptane or pyridine, or combination of them. PQQ, which had been adsorbed to Sep-Pak C18 cartridges, could be eluted with a mixture of pyridine and water with very excellent recovery. The recovery of 1 micrograms PQQ, which had been added to 1 g human liver, brain and 1 ml plasma and had undergone the n-butanol and the Sep-Pak extractions, was 50, 75 and 105%, respectively. From the blank fluorescence, endogenous levels of free PQQ in human liver, brain and plasma were found not greater than 0.41, 0.08 and 0.13 micrograms/g or ml, respectively, if present.
journal_name
Life Scijournal_title
Life sciencesauthors
Suzuki O,Kumazawa T,Seno H,Urakami T,Matsumoto Tdoi
10.1016/0024-3205(90)90313-gsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
2135-41issue
23eissn
0024-3205issn
1879-0631pii
0024-3205(90)90313-Gjournal_volume
47pub_type
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