Abstract:
:Immunotherapy methods using potential tumor microenvironment modulators have elicited durable therapeutic responses in cancer treatment. Immune checkpoint molecule programmed cell death-ligand 1 (PD-L1) and oncogenic transcription factor STAT3 (signal transducer and activator of transcription-3) assigned as inhibitory targets of our study and particular delivery system designed to deliver small interfering RNAs (siRNAs) to silence the targeted genes. Generated trimethyl chitosan (TMC) and thiolated chitosan (TC) nanoparticles (NPs) conjugated with HIV-1-derived TAT peptide and HA (hyaluronic acid) exhibited eligible physicochemical characteristics, notable siRNA encapsulation, serum stability, non-toxicity, controlled siRNA release, and extensive cellular uptake by cancer cells. Dual inhibition with STAT3/PD-L1 siRNA-loaded HA-TAT-TMC-TC NPs led to promising results, including significant downregulation of PD-L1 and STAT3 genes, striking suppressive effects on proliferation, migration, and angiogenesis of breast and melanoma cancer cell lines, and restrained tumor growth in vivo. These findings infer the capability of HA-TAT-TMC-TC NPs containing STAT3/PD-L1 siRNAs as a novel tumor-suppressive candidate in cancer treatment.
journal_name
Life Scijournal_title
Life sciencesauthors
Bastaki S,Aravindhan S,Ahmadpour Saheb N,Afsari Kashani M,Evgenievich Dorofeev A,Karoon Kiani F,Jahandideh H,Beigi Dargani F,Aksoun M,Nikkhoo A,Masjedi A,Mahmoodpoor A,Ahmadi M,Dolati S,Namvar Aghdash S,Jadidi-Niaragh Fdoi
10.1016/j.lfs.2020.118847subject
Has Abstractpub_date
2021-02-01 00:00:00pages
118847eissn
0024-3205issn
1879-0631pii
S0024-3205(20)31600-3journal_volume
266pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::A microiontophoretic study using rats anesthetized with chloral hydrate and immobilized with gallamine triethiodide was carried out to compare the effect of talipexole (B-HT 920 CL2:2-amino-6-allyl-5,6,7,8-tetrahydro-4H-thiazolo [4,5-d]-azepine-dihydrochloride), a dopamine autoreceptor agonist, on dopaminergic neurons...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(91)90071-i
更新日期:1991-01-01 00:00:00
abstract:AIMS:The potential of human mesenchymal stem cell-like stroma prepared from placental/umbilical cord blood for hematopoietic regeneration by X-irradiated hematopoietic stem cells is herein assessed. MAIN METHODS:Placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells were applied to a regenerat...
journal_title:Life sciences
pub_type: 杂志文章
doi:
更新日期:2009-04-24 00:00:00
abstract::Human T-cell lymphotropic virus type 1 (HTLV-1) is associated with adult T-cell leukemia/lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Only a limited percentage of infected individuals develop disease in response to the virus while the majority remain asymptomatic, and HAM/TSP...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2014.03.025
更新日期:2014-05-28 00:00:00
abstract::The purpose of this study was to investigate the involvement of calcium in K+ currents and its effects on amino acid accumulation and on the membrane potential regulated by tri-iodo-L-thyronine (T3) in Sertoli cells. Immature rat testes were pre-incubated for 30 min in Krebs-Ringer bicarbonate buffer and incubated for...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2003.08.005
更新日期:2004-01-23 00:00:00
abstract::Recent findings indicate that low concentrations of dopamine (DA) stimulate the secretion of prolactin (PRL) in vitro. In this study, we found that low concentrations of the highly-specific DA D2 receptor agonist, quinpirole hydrochloride (LY) stimulate PRL secretion in female rats, assessed by reverse hemolytic plaqu...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90749-s
更新日期:1993-01-01 00:00:00
abstract::Simultaneously drawn arterial and forearm venous levels of free and sulfate conjugated epinephrine and norepinephrine were measured in 8 subjects in order to study the role of skeletal muscle in catecholamine sulfation. A sensitive radioenzymatic assay was used. The average free epinephrine level was 46% lower in veno...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90758-5
更新日期:1982-11-29 00:00:00
abstract::Basic fibroblast growth factor (bFGF) has a neurotrophic effect both in vitro and in vivo, and is considered to play an important role in the maintenance of neuronal functions in the normal brain. Neural damage in brain contusion progresses after the primary injury of trauma because of cerebral hemodynamic and metabol...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00332-7
更新日期:1994-01-01 00:00:00
abstract::Nitric oxide (NO) plays an important role in glucose and lipid metabolism. We previously reported that NO synthesis inhibitors, such as NG-nitro-L-arginine methyl ester (L-NAME), deteriorate insulin sensitivity and lipid metabolism, while the addition of L-arginine reverses this deterioration. L-arginine is a precurso...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2003.06.004
更新日期:2003-10-24 00:00:00
abstract:AIMS:Rg1 is the most active component of traditional Chinese medicine ginseng, having anti-aging and anti-oxidative stress features in multiple organs. Cellular senescence of hepatocytes is involved in the progression of a wide spectrum of chronic liver diseases. In this study, we investigated the potential benefits an...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117776
更新日期:2020-08-01 00:00:00
abstract::Nuclear factor erythroid 2-related factor 2 (Nrf-2) binds to a specific polyamine responsive element (PRE) in the promoter region of the spermidine-spermine acetyltransferase (SSAT) gene, a key component of the polyamine catabolic pathway. Regulation of SSAT gene transcription requires the additional interaction of Nr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.03.042
更新日期:2006-08-22 00:00:00
abstract::Estrogen can enhance or reduce lymphocyte functions in vitro depending on dose and exposure duration. The purpose of this study was to determine the effect of in vivo 17 beta-estradiol (E2) on apoptosis and necrosis in lymphoid tissue of female C567BL/6 mice. Animals were ovariectomized (OVX), ovariectomized and 17 be...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(00)00456-2
更新日期:2000-03-03 00:00:00
abstract:AIMS:The prognoses of patients with gastric cancer(GC) vary in different stages, which is mainly due to the great differences in tumor and tumor microenvironment. This study is aimed to explore the specific differences. MAIN METHODS:Based on RNAseq-based expression data from The Cancer Genome Atlas database and GSE154...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118402
更新日期:2020-11-15 00:00:00
abstract:AIMS:This study sought to determine the impact of in vitro exposure to the herbicide atrazine (ATR) or its major mammalian metabolite diaminochlorotriazine (DACT) on dopaminergic cell differentiation. MAIN METHODS:N27 dopaminergic cells were exposed for 24 or 48 h to ATR or DACT (12-300 μM) and their effects on cell v...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.10.027
更新日期:2013-01-17 00:00:00
abstract:AIMS:Gender difference in cardiac ischemia-reperfusion (IR) induced injury has been reported in animal models. However, a large-scale clinical trial found an increase in cardiovascular incidents in women with hormone replacement therapy. The present study is aimed to explore possible mechanisms of gender difference in ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.06.019
更新日期:2013-08-28 00:00:00
abstract::There is an augmented platelet intracellular calcium response to serotonin stimulation in major depression. The role that calcium influx has in this process is not known. The objective of this study was to determine platelet calcium influx in response to serotonin by two methods, Mn2+ influx and 45Ca2+ uptake, in orde...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00608-6
更新日期:2000-01-01 00:00:00
abstract::To analyze the effects of diabetes mellitus on the vascular responsiveness to nitric oxide and thromboxane receptor stimulation, 2 mm long segments of basilar, coronary, renal and tail arteries from male and female, control (normoglycemic) and streptozotocin-induced diabetic rats, were prepared for isometric tension r...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02444-x
更新日期:2003-02-14 00:00:00
abstract::Male, 5 months old, massively obese, spontaneously hypertensive rats (Obese/SHR) were given 10 mg alloxan/100 g b.w., s.c., to induce diabetes. Control Obese and non-obese/SHR were given saline. Insulin therapy was withheld. All of the animals were killed at 6 months of age. Alloxan caused a slight but statistically s...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90666-5
更新日期:1983-09-12 00:00:00
abstract::The lymphatic vessels have been implicated in maintenance of interstitial fluid homeostasis and immune responses, and pathological conditions including inflammation, wound healing, lymphedema and tumor progression. The knowledge about the lymphatic structure and function in muscles, especially in muscular disorders, r...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2018.09.043
更新日期:2018-10-15 00:00:00
abstract::The process of synaptogenesis has been studied by many investigators to determine the factors which regulate synapse formation. We have used neonatal rabbit retina to investigate the role of the gamma-aminobutyric acid (GABA) neurotransmitter system during development. By utilizing an in vitro incubation treatment of ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90754-3
更新日期:1983-09-05 00:00:00
abstract::The specific binding of 35S-t-butylbicyclophosphorothionate (TBPS) was studied in synaptosomal membranes of rat cerebral cortex. The displacing potencies of eleven CNS depressants and three convulsants were determined in the presence of 1 microM GABA and 10 nM R 5135. GABA enhanced the displacing potencies of depressa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90589-9
更新日期:1988-01-01 00:00:00
abstract::The purpose of this study was to investigate the effect of T3 on amino acid accumulation and on the membrane potential of Sertoli cells of immature rat testes. Testes of pre-pubertal and pubertal rats were pre-incubated (30 min) in Krebs-Ringer bicarbonate buffer and incubated in the presence of [14C]methylaminoisobut...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01186-9
更新日期:2001-07-13 00:00:00
abstract::Endothelin-1 (ET-1) has been proposed as one of the possible mediators of the vasoconstriction seen following ischemia and reperfusion. We investigated the effect of ischemia and reperfusion on the contractile response of canine renal and iliac arteries to the dihydropyridine-type calcium channel agonist (+/-)Bay K 86...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(95)00267-a
更新日期:1995-01-01 00:00:00
abstract::Effects of hexamethonium (C6) on the excitability of sympathetic ganglion cells were examined by means of intracellular recording. When DC currents were injected, high concentrations of C6 significantly augmented the repetitive firing of the cells without any change in threshold voltage for initiation of the spike. Th...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90324-8
更新日期:1985-06-17 00:00:00
abstract::A fostering/crossfostering analysis of the effects of maternal ethanol exposure on jejunal and ileal folate absorption was performed. Male and female rats were randomized into two groups. In the first group, ethanol-treated rats received ad libitum 5, 10 and 15% ethanol in the drinking fluid during three successive we...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(03)00570-8
更新日期:2003-09-12 00:00:00
abstract::The effects of 100 microM norepinephrine (NE), GABA, aspartate, glutamate, and carbachol on the release of endogenous NE, GABA, aspartate, and glutamate from slices of rat cerebellum were examined. The 35 mM K+-stimulated release of NE was potentiated by GABA (136% of control), glutamate (123%), and carbachol (123%); ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90308-5
更新日期:1986-01-20 00:00:00
abstract::More than 140 thousands of women suffer from endometrial carcinoma in the worldwide, and over 40 thousand of the patients die before and after in surgery and chemoradiotherapy because of its metastasis. However, its molecular mechanism is much less known compared to other cancers. In this study, we demonstrated that l...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.117295
更新日期:2020-02-15 00:00:00
abstract::Decreased heart rate variability (HRV) and heart rate turbulence (HRT) are independent predictors of mortality after acute myocardial infarction (AMI). There are no previous studies on the relationship between warm-up phenomenon and cardiac autonomic control in stable coronary artery disease (CAD). We investigated the...
journal_title:Life sciences
pub_type: 临床试验,杂志文章
doi:10.1016/j.lfs.2004.06.033
更新日期:2005-03-25 00:00:00
abstract::Alteration in microbial populations and metabolism are key events associated with disruption of intestinal homeostasis and immune tolerance during intestinal inflammation. A substantial imbalance in bacterial populations in the intestine and their relationships with the host have been observed in patients with inflamm...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2018.11.009
更新日期:2018-12-15 00:00:00
abstract::Pretreatment with a low dose of 3-nitropropionic acid (3-NPA) has been shown to induce ischemic tolerance in the gerbil hippocampus. It is well known that sublethal (2-min) ischemia also induces ischemic tolerance. To investigate the acquisition of ischemic tolerance with 3-NPA, we examined the protein expression afte...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.01.037
更新日期:2005-10-21 00:00:00
abstract::Lipocalin2 (Lcn2) has been shown to be a vital regulator of tumorigenesis in a variety of different cancers. However, its expression patterns and possible roles in ovarian cancer remain obscure. The aim of this study was to investigate the expression of Lcn2 in ovarian cancer cells and to determine any potential assoc...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118492
更新日期:2020-12-01 00:00:00