Abstract:
:Nuclear factor erythroid 2-related factor 2 (Nrf-2) binds to a specific polyamine responsive element (PRE) in the promoter region of the spermidine-spermine acetyltransferase (SSAT) gene, a key component of the polyamine catabolic pathway. Regulation of SSAT gene transcription requires the additional interaction of Nrf-2 with polyamine modulated factor 1 (PMF-1). Likewise, transcription of the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) gene is regulated in a polyamine-dependent manner, but the actual mechanism has not previously been determined. Analysis of the 5'-flanking sequence of the murine 4E-BP1 gene indicated the presence of several potential PRE sites, which might be involved in regulating its transcription. Our goal in this research was to determine potential interactions between Nrf-2, PMF-1, the human homologue of the Arabidopsis signalosome complex (CSN-7), and these potential PRE sites. Four PCR fragments containing regions with considerable homology (78%) to the human PRE were generated from the 5'-flanking sequence of the mouse 4E-BP1 gene and the fragments were used in electrophoretic gel mobility shift and supershift assays. Purified Nrf-2 interacted with all four of these fragments, and similar gel shifts were observed with both cytoplasmic and nuclear fractions of NIH-3T3 cells. However, polyamine depletion with difluoromethylornithine (DFMO) eliminated the gel shift. Supershift assays indicated that the shift was due to the binding of Nrf-2, and the binding was competitive with a known Nrf-2 binding sequence. Purified PMF-1 did not bind any of the PCR fragments alone, but when added with Nrf-2, decreased the magnitude of the gel shift for one of the fragments (PRE located at -2060 relative to the transcription start site). CSN-7 did not interact with the sequences, nor did it inhibit protein/DNA interaction. These data indicate a possible mechanism by which polyamines enhance the binding of a Nrf-2/PMF-1 complex to the 5'-flanking region of the 4E-BP1 gene. Since polyamines increase expression of the 4E-BP1 gene, it seems likely that formation of this complex is involved in its transcriptional regulation.
journal_name
Life Scijournal_title
Life sciencesauthors
Stephenson AH,Seidel ERdoi
10.1016/j.lfs.2006.03.042subject
Has Abstractpub_date
2006-08-22 00:00:00pages
1221-7issue
13eissn
0024-3205issn
1879-0631pii
S0024-3205(06)00295-5journal_volume
79pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::A number of experimental studies report that biological systems can be affected by in vivo exposure to low frequency and extremely low frequency electromagnetic fields. However, attempts to independently replicate some of these studies have shown the reported effects to be elusive. The difficulty in replicating result...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(97)00770-4
更新日期:1997-01-01 00:00:00
abstract:AIMS:Individual susceptibility to develop obesity may impact the development of cardio-metabolic risk factors that lead to obesity-related comorbid conditions. Obesity-prone Osborne-Mendel (OM) rats expressed higher levels of visceral adipose inflammation than obesity-resistant, S5B/Pl (S5B) rats. However, the consumpt...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.03.022
更新日期:2019-04-15 00:00:00
abstract::The binding of different gangliosides to rat T-helper lymphocytes was characterized under conditions that decrease CD4 expression on different mammalian T-helper lymphocytes. Saturation binding by monosialylated [3H]-GM1 to rat T-lymphocytes was time- and temperature-dependent, had a dissociation constant (KD) of 2.2 ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90123-9
更新日期:1989-01-01 00:00:00
abstract:AIMS:Diabetes increases oxidant stress and impairs endothelium-dependent relaxation. We investigated whether the antioxidant 3',4'-dihydroxyflavonol (DiOHF) reduces the release of superoxide (O(2)(-)) and preserves endothelial function in aortae from diabetic rats. MAIN METHODS:Type-1 diabetes was induced in Sprague-D...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.04.012
更新日期:2009-07-03 00:00:00
abstract:AIMS:Naturally-derived farnesol has been reported for its chemopreventive and chemotherapeutic efficacy in various cancers. However, the mechanism of action of farnesol is still to be elucidated. The present study demonstrates the chemopreventive potential of farnesol on 9,10-dimethylbenz(a)anthracene (DMBA)-initiated ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2009.05.008
更新日期:2009-07-31 00:00:00
abstract::Sweat glands are innervated by sympathetic neurons which undergo a change in transmitter phenotype from noradrenergic to cholinergic during development. As soon as the glands begin to differentiate, M3 muscarinic receptor mRNA and binding sites are detectable. Receptor expression appears in the absence of innervation ...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/s0024-3205(98)00578-5
更新日期:1999-01-01 00:00:00
abstract::In the course of investigating the binding of imipramine to soluble cellular fractions from brain and leukocytes using equilibrium dialysis, an artifactual binding component was produced. On Scatchard and double-reciprocal plots of the data, the component appeared as a homogenous population of sites displaying a bindi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90405-8
更新日期:1983-07-11 00:00:00
abstract::Exposure of rats to an acute, uncontrollable stressor can increase brain norepinephrine (NE) turnover and decrease locomotor and exploratory behavior. We examined the ability of exogenous tyrosine, NE's amino acid precursor, to protect rats from developing these neurochemical and behavioral changes when stressed. Anim...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90209-1
更新日期:1984-06-04 00:00:00
abstract::To study the role of IL-5 in allergic airway hyperreactivity, the time course for the production of cytokines, the infiltration of inflammatory cells and the onset of airway hyperreactivity after three inhalations of antigens were studied in mice. The effect of the soluble alpha-chain of murine recombinant interleukin...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)90139-2
更新日期:1994-01-01 00:00:00
abstract::NSAIDs can elevate blood pressure through mechanisms such as renal vasoconstriction and sodium retention. These effects are particularly evident in hypertensive individuals. Nitric oxide-releasing NSAID derivatives have been shown to have greatly reduced toxicity in the gastrointestinal tract and kidney. We therefore ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00072-1
更新日期:1998-01-01 00:00:00
abstract::Uptake of 131iodine-metaiodobenzylguanidine (131I-MIBG) by 6-23 rat medullary thyroid carcinoma (MTC), was studied in vitro and in vivo. In vitro, there was an 8-fold increase in 131I uptake by 6-23 cells when labeled with 131I-MIBG (131I 24 +/- 15 cpm/10(6) cells, 131I-MIBG 196 +/- 9 cpm/10(6) cells). MIBG uptake in ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90456-6
更新日期:1989-01-01 00:00:00
abstract::Hypoxic preconditioning (HPC) has been well demonstrated to have potent protective effects in many cell types; however, the mechanisms responsible for this phenomenon are not fully understood. Recently, glucose-regulated protein 78 (GRP78), an inducible molecular chaperon, was indicated to be associated with ischemic ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.08.015
更新日期:2007-09-08 00:00:00
abstract::The extended infection with Helicobacter pylori (H. pylori), one of the most frequent infectious agents in humans, may cause gastritis, peptic ulcers, gastric mucosa-associated lymphoid tissue (MALT) lymphoma, and gastric cancer. During H. pylori infection, different kinds of inflammatory cells such as dendritic cells...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2019.116688
更新日期:2019-08-15 00:00:00
abstract::Aim Furowanin A (Fur A) is a flavonoid isolated from Millettia pachycarpa Benth. Studies show its potent anti-neoplastic effects against leukemia cells. The aim of the present study was to determine the potential therapeutic effect of Fur A against colorectal cancer (CRC), and elucidate the underlying mechanism. MATE...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.12.027
更新日期:2019-02-01 00:00:00
abstract::Rhesus monkeys were trained to discriminate injections of saline from those of beta-carboline-3-carboxylic acid ethyl ester (beta-CCE), a compound that binds to the benzodiazepine receptor, but often has actions opposite to those of the benzodiazepines. A benzodiazepine agonist midazolam and low doses of a specific be...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90240-7
更新日期:1986-03-17 00:00:00
abstract::Aim Doxorubicin (Dox) is one of the most cardiotoxic anti-cancerous drug that is widely used for broad-range of cancers. There is an urgent need for developing cardio-oncological therapeutic interventions. Natural products having both anti-cancerous potential as well as cardioprotective effects may hold a great potent...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.05.021
更新日期:2018-07-15 00:00:00
abstract::Carbachol (CCH), serotonin (5HT), divalent ionophore A23187, cAMP, and certain neuropeptides, i.e. substance P (SP), inhibit the initial rate of uptake (influx) of 22Na into isolated chicken villus enterocytes. All these agents also increase cytosolic Ca. However, the increases stimulated by CCH, 5HT, and cAMP are not...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(90)90506-m
更新日期:1990-01-01 00:00:00
abstract::Chronopharmacokinetics deals with the study of the temporal changes in absorption, distribution, metabolism and elimination and thus takes into account the influence of time of administration on these different steps. In the last decade, numerous studies have been devoted to chronokinetics: recent advances will be rev...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(93)90001-j
更新日期:1993-01-01 00:00:00
abstract::The effect of gamma-aminobutyric acid (GABA) on the binding of PK 8165, a quinoline derivative, and CGS 8216, a pyrazoloquinoline, was assessed in two different regions of the rat brain. PK 8165, a compound with reported anxiolytic properties, inhibited [3H]-propyl beta-carboline-3-carboxylate labeled receptors in the...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90403-9
更新日期:1982-07-05 00:00:00
abstract::The accumulation of lysophosphoglycerides has been implicated as an important biochemical factor for cardiac arrhythmias. Recently, we demonstrated that lysophosphatidylcholine caused cardiac arrhythmias in the isolated hamster heart. In this study, the arrhythmogenic nature of various lysophosphoglycerides with respe...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90806-8
更新日期:1983-03-21 00:00:00
abstract::The effects of two benzodiazepine derivatives (diazepam, 0.5-1 mg/kg; alprazolam, 1.25-2.5 mg/kg) on ultrasonic calling elicited in adult rats by unavoidable aversive stimuli (footshocks) were investigated. The results show that either diazepam or alprazolam affected the duration of ultrasonic calls. In particular, a ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90149-x
更新日期:1988-01-01 00:00:00
abstract:AIMS:Mild traumatic brain injury (mTBI) is an important risk factor for cognitive impairment. Despite intense efforts to develop efficient treatments, the current therapies are not often effective and far from satisfactory. Silymarin has been suggested as a therapeutic agent in the treatment of traumatic brain injury. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118049
更新日期:2020-09-15 00:00:00
abstract::Intravenous injection of prostacyclin (100 micrograms/kg) in rats resulted in a decrease of systolic blood pressure within 2 minutes. Concentrations of cAMP in 15 brain regions and nuclei were determined by radioimmunoassay. In lower brain stem nuclei, such as the nucleus of the solitary tract and the lateral reticula...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90439-5
更新日期:1987-12-07 00:00:00
abstract:AIMS:Cancer Stem Cells (CSCs) refers to heterogeneous tumor cells retaining the abilities of self-renewal and differentiation. This study used mRNAsi, which is an index to describe the similarity between tumor cells and CSCs, to define genes involved in endometrial carcinoma. MATERIALS AND METHODS:The mRNA expression ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.118231
更新日期:2020-10-01 00:00:00
abstract::Osteoarthritis (OA) is a complex joint disorder with a number of underlying physical, biochemical, biomechanical and genetic causes. Obesity is considered to be one of the major risk factors for the development and progression of OA. It actively contributes to the inflammatory status and to cartilage degradation in th...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2015.05.025
更新日期:2015-11-01 00:00:00
abstract::Aluminum (Al) is thought to be a risk factor for neurodegenerative disorders, but the molecular mechanism has been not clarified yet. In this study, we examined how a transport system handled transport of Al citrate, the major Al species in brain, and effect of Al citrate treatment on expression of the transporter and...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.12.048
更新日期:2006-05-30 00:00:00
abstract::In vitro studies showed that organophosphate insecticides have different IC50 values (i.e., concentration of inhibitor required to inhibit 50% enzyme activity) for acetylcholinesterases (AChE's) from different species. Since previous reports indicated that the binding of active cholinesterase inhibitors to non-critica...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90426-x
更新日期:1982-07-12 00:00:00
abstract::We compared the relative abilities of atropine sulfate and methylatropine, injected i.v. and into the cerebral ventricles (icv), to block pharmacological responses mediated through central and peripheral muscarinic receptors. The hypotensive response to i.v. injection of acetylcholine (peripheral muscarinic receptors)...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90389-x
更新日期:1988-01-01 00:00:00
abstract::The ontogenetic development of [3H]-spiroperidol binding sites was measured in the optic tectum, cerebellum, forebrain base, and forebrain roof of 1-, 4-, and 16-day-old chicks. In the chick optic tectum and cerebellum both the density and the total number of [3H]-spiroperidol binding sites increased from 4- to 16-day...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90331-7
更新日期:1989-01-01 00:00:00
abstract::The binding of the D2-like agonists, (+)-7-hydroxy-N,N-di-n-[3H]propyl-2-aminotetralin (7-OH-DPAT) and [3H]pramipexole (2-amino-4,5,6-tetrahydro-6-propylaminobenzthiazole; MIRAPEX) were determined in membranes from adult male Sprague-Dawley and Fischer-344 rats. Saturation analysis, which optimized binding to D3 recep...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00446-9
更新日期:1998-01-01 00:00:00