Abstract：
AIMS:Diabetes increases oxidant stress and impairs endothelium-dependent relaxation. We investigated whether the antioxidant 3',4'-dihydroxyflavonol (DiOHF) reduces the release of superoxide (O(2)(-)) and preserves endothelial function in aortae from diabetic rats. MAIN METHODS:Type-1 diabetes was induced in Sprague-Dawley rats by streptozotocin (STZ) treatment (55 mg/kg i.v.) and vascular reactivity and superoxide generation were assessed in aortic rings using standard organ bath techniques and lucigenin-enhanced chemiluminescence respectively. KEY FINDINGS:Eight weeks after STZ treatment blood glucose was elevated (39.4+/-0.4 mM) compared to citrate treated control rats (5.5+/-0.1 mM, P<0.05) and there was an increased aortic generation of O(2)(-) (control 670+/-101, diabetic 1535+/-249 units/mg dry weight, P<0.05). In aortic rings acetylcholine (ACh)-induced relaxation was impaired (R(max) control 78+/-2, diabetic 66+/-3%, P<0.01) whereas endothelium-independent relaxation to sodium nitroprusside (SNP) was unaffected (R(max) control 100+/-1, diabetic 101+/-2%). When aortic rings were acutely exposed to DiOHF (10(-5) M) there was a significant reduction in the detection of O(2)(-) (control 124+/-15, diabetic 165+/-21 units/mg, P<0.01) and enhanced relaxation to ACh (R(max) control 84+/-3, diabetic 87+/-3%). Two separate groups of rats (control and diabetic) were treated daily with DiOHF (5 mg/kg i.p.) for 7 days. DiOHF treatment reduced superoxide generation in diabetic aortae (untreated diabetic 1471+/-358, DiOHF-treated diabetic 580+/-115 units/mg, P<0.05) and enhanced acetylcholine-induced relaxation (R(max) untreated diabetic 58+/-5, DiOHF-treated diabetic 71+/-4%, P<0.05). SIGNIFICANCE:DiOHF, acutely in vitro or after 1 week treatment in vivo, reduces oxidant stress and preserves endothelium-dependent relaxation in aortae from diabetic rats.
journal_name
Life Scijournal_title
Life sciencesauthors
Woodman OL,Malakul Wdoi
10.1016/j.lfs.2009.04.012subject
Has Abstractpub_date
2009-07-03 00:00:00pages
54-9issue
1-2eissn
0024-3205issn
1879-0631pii
S0024-3205(09)00178-7journal_volume
85pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Hypoxia-induced neuronal damage and glutamate release were investigated in a N(2)- or in xenon-atmosphere for embryonic rat cortical neurons; cellular damage and glutamate over-release were observed in N(2)-treated cells whereas xenon protected the cells from the hypoxic insult. The protective effect of xenon was stro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)02439-6
更新日期：2003-03-14 00:00:00
abstract：:The effect of resveratrol, a polyphenolic compound present in grapes and other plants, on proteinuria, hypoalbuminemia and hyperlipidemia was studied in rats with glomerulonephritis. The nephritis was induced by an intravenous injection of anti-rat kidney glomerular basement membrane rabbit antiserum. Nephritic rats w...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(01)01061-x
更新日期：2001-05-11 00:00:00
abstract：:In our previous studies, exposure of mice to millimeter waves (MW) increased the duration of anesthesia caused by either ketamine or chloral hydrate, and this effect was blocked by naloxone. To further characterize the biological effects of MW, we have chosen a new animal model of experimental itch. Male Swiss albino ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00436-6
更新日期：1998-01-01 00:00:00
abstract：AIMS:Tianeptine is an atypical antidepressant drug that has a different mechanism of action than other antidepressants. Olanzapine is an atypical antipsychotic drug used for the treatment of schizophrenia. The present study was undertaken to investigate effects of chronic administration of tianeptine or olanzapine on u...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.09.023
更新日期：2012-12-17 00:00:00
abstract：:The O2 affinity of "stripped" (cofactor-free) hemoglobin (Hb) of the elasmobranch, Squalus acanthias is decreased by ATP, the main erythrocytic phosphate cofactor but increased by urea at physiological concentration. When both compounds are present, as in life, urea decreases the ATP sensitivity, indicating that previ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90105-4
更新日期：1983-05-02 00:00:00
abstract：:Although several reports imply that anticoagulants and preservatives contain zinc, the quantity of zinc in heparin, if any, has not been documented. Zinc concentration was determined by flame atomic absorption spectroscopy in varying dilutions of multiple commercially obtained samples of purified sodium heparin N = 15...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90348-x
更新日期：1983-12-26 00:00:00
abstract：AIMS:The aim of the study was to identify key miRNAs related to hepatocellular carcinoma (HCC) and then to explore their potential function and clinical significance. MATERIALS AND METHODS:The miRNA expression profiles of 387 HCC and 62 normal liver tissues were obtained from the Gene Expression Omnibus (GEO) and The ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.116596
更新日期：2019-09-01 00:00:00
abstract：:This study was designed to document possible changes in bupivacaine and its main metabolite, des-butyl-bupivacaine (PPX) kinetics in mice after a single 5 mg/kg injection of diltiazem. After a single 20 mg/kg i.p. dose of bupivacaine, kinetic parameters of bupivacaine were not statistically different but the ratio AUC...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00601-6
更新日期：1994-01-01 00:00:00
abstract：:In order to better understand the mechanisms underlying the reduction in GH secretion in diabetic rats, we have characterized and measured SRIH receptors in the hypothalamus and anterior pituitary gland 5 and 9 days after induction of diabetes in the rat. Experimental diabetes was induced by an intraperitoneal injecti...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)90151-1
更新日期：1994-01-01 00:00:00
abstract：:We have examined the role of membrane hyperpolarization in mediating vascular hyporeactivity induced by bacterial lipopolysaccharide (LPS) in endothelial-denuded strips of rat thoracic aorta ex vivo. The injection of rats with LPS caused a significant fall of blood pressure and a severe vascular hyporeactivity to nore...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00978-4
更新日期：2000-12-29 00:00:00
abstract：:The cellular electrophysiology of left ventricular preparations from guinea pig hearts was studied 1 hour, 24 hours, and 4-6 weeks after myocardial infarction produced by 6-8 single ties of the distal left coronary artery system or after sham operation. Microelectrode recordings were used to monitor cells from the end...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90343-1
更新日期：1985-06-24 00:00:00
abstract：:14C-Imipramine was administered to male and female Sprague-Dawley rats and animals were sacrificed at 4, 8, 12, and 20 hours later. At all time points, total radioactivity in female lung was several-fold higher than in males. In addition, female lungs had a higher concentration of desmethylimipramine (DMI) as compared...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90610-5
更新日期：1982-03-01 00:00:00
abstract：:Alterations in the cyclic AMP-dependent protein kinase activity ratio in response to putative neurotransmitters and other cyclic AMP-elevating agents in intact cerebral cortical slices and Krebs-Ringer particulate preparations from cerebral cortex were examined. Both norepinephrine (30 microM) and forskolin (20 microM...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90629-0
更新日期：1985-07-08 00:00:00
abstract：:The traditional Chinese medicines have been used clinically for a long time in some Asian countries, however, very few studies have been done to demonstrate the working mechanisms of these medicines using recently developed biochemical methodologies. In this study, we examined the anti-inflammatory effect of Huang-Lia...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00557-3
更新日期：2000-01-01 00:00:00
abstract：:The platelet activating factor (PAF) receptor of the guinea pig peritoneal eosinophil was characterized by radioligand ([3H]WEB 2086) binding and measurement of PAF-stimulated membrane GTPase activity. Specific binding of [3H]WEB 2086 was rapid, reversible and saturable, with an equilibrium KD of 20.4 nM at 0 degrees ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(93)90045-5
更新日期：1993-01-01 00:00:00
abstract：:Levels of delta-sleep-inducing peptide-like immunoreactivity (DSIP-LI) in rat plasma were measured by radioimmunoassay and found to exhibit a circadian rhythm that parallelled the normal rhythm for corticosterone. The maximal plasma levels of both substances were observed to occur at about 1700h. The lowest concentrat...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90566-6
更新日期：1984-11-12 00:00:00
abstract：:The effect of polyamines on the catalytic domain of protein kinase C from rat brain was investigated. It was found that the addition of spermine strongly inhibited phosphorylation activity toward histone H1 as substrate. This tetramine, at millimolar concentrations, was most potently effective while triamines and diam...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(88)90382-7
更新日期：1988-01-01 00:00:00
abstract：:This study was designed to evaluate the synergistic cytotoxicity of herb prescription, Palgin, in adriamycin-treated cancer cells. The combination of Palgin and adriamycin synergistically augmented the cytotoxicity of Chang and HL-60 cells, but not in Hep3B and Alexander cells. The cytotoxicity of two drugs was reveal...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)02039-8
更新日期：2002-10-04 00:00:00
abstract：:The Wiskott-Aldrich syndrome (WAS), X-linked severe combined immunodeficiency (SCIDX1), and X-linked agammaglobulinemia (XLA) are severe congenital immunodeficiencies with X-linked inheritance. Although rare, they are all associated with severe infections from early in life, and high morbidity and mortality. Female ca...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00686-3
更新日期：1997-01-01 00:00:00
abstract：:Benzo(a)pyrene induces cytochrome P-450 1A1 (CYP1A1) expression in rat polymorphonuclear leukocytes (PMNs) that upregulates expression of inducible nitric oxide synthase (iNOS). In the present study, the involvement of secondary signaling molecules in CYP1A1-mediated augmentation of iNOS expression in benzo(a)pyrene-t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.09.032
更新日期：2007-11-30 00:00:00
abstract：:The aim of this study was to verify, by means of functional methods, whether the circadian rhythm changes adrenergic response patterns in the epididymal half of the vas deferens isolated from control rats as well as from rats submitted to acute stress. The experiments were performed at 9:00 a.m., 3:00 p.m., 9:00 p.m.,...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.02.012
更新日期：2005-08-26 00:00:00
abstract：:Specific 125I-CCK receptor binding was significantly increased in brain tissue taken from guinea pig or mouse following chronic (2-3 week) daily administration of haloperidol (2-3 mg/kg/day). Scatchard analysis indicated the increase in CCK binding was due to an increased receptor number (B max) with no change in affi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90224-2
更新日期：1983-02-21 00:00:00
abstract：:The distribution of thiamine-immunoreactive structures was studied in the brain of the monkey using an indirect immunoperoxidase technique. Fibers containing thiamine, but no thiamine-immunoreactive cell bodies, were found. The highest density of fibers containing thiamine was observed in the pulvinar nucleus and in t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.03.017
更新日期：2006-08-15 00:00:00
abstract：:In this paper, we describe the characterization of the expression of monoamine oxidase (MAO) in whole pancreas and in isolated islets of Langerhans from human. Classical monamine oxidase activity assays reveal that both isoforms A & B are present in human pancreas. Two complementary approaches indicated that both MAO ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00265-9
更新日期：1999-01-01 00:00:00
abstract：AIMS:β-Adrenoceptors (β-ADRs) mediating the relaxation of rat superior mesenteric arteries (SMAs) were pharmacologically identified, and the effects of chemical sympathetic denervation on β-ADR-mediated relaxation were examined. MAIN METHODS:The tension changes of endothelium-denuded SMAs were isometrically recorded a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.117155
更新日期：2020-01-15 00:00:00
abstract：AIMS:Oxaliplatin (l-OHP) is a key drug in therapeutic regimens for metastatic or advanced-stage colorectal cancer, but causes peripheral neuropathy as a dose-limiting adverse effect. It is reported that this peripheral neuropathy results from l-OHP accumulation in dorsal root ganglion (DRG) neurons, and that one of the...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.07.006
更新日期：2018-08-15 00:00:00
abstract：AIMS:To design and screen a potent GLP-1/GIP/Gcg receptors triagonist with therapeutic potential in rodent animals with diabetes and obesity. MAIN METHODS:First, we obtained a 12-mer dual GIP/Gcg receptor agonist from a large combinatorial peptide library via high-throughput screening technique and then fused to the E...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118339
更新日期：2020-11-01 00:00:00
abstract：:The hypothalamic AMP-activated protein kinase (AMPK)/acetyl-CoA carboxylase (ACC) pathway is known to play an important role in the control of food intake and energy expenditure. Here, we hypothesize that citrate, an intermediate metabolite, activates hypothalamic ACC and is involved in the control of energy mobilizat...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2008.04.015
更新日期：2008-06-20 00:00:00
abstract：:Muscarinic receptors are expressed in smooth muscle throughout the body. In most instances, the muscarinic receptor population in smooth muscle is composed of mainly the M2 and M3 subtypes in an 80% to 20% mixture. The M3 subtype mediates phosphoinositide hydrolysis and calcium mobilization, whereas the M2 subtype med...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(97)00433-5
更新日期：1997-01-01 00:00:00
abstract：:Neuropathic pain syndromes respond poorly to opioid treatment. In our previous studies, we found that intrathecal (i.t.) injection of pertussis toxin (PTX) produces thermal hyperalgesia, which is poorly responsive to morphine and is accompanied by an increase in cerebrospinal fluid (CSF) levels of excitatory amino aci...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.08.020
更新日期：2006-03-13 00:00:00
