Abstract:
:In this paper, we describe the characterization of the expression of monoamine oxidase (MAO) in whole pancreas and in isolated islets of Langerhans from human. Classical monamine oxidase activity assays reveal that both isoforms A & B are present in human pancreas. Two complementary approaches indicated that both MAO A and B are expressed in isolated islet: RT-PCR using specific primers revealed amplification products with the expected size for MAO-A and MAO-B: two peptides corresponding to MAO A (approximately 61 kDa) and B (approximately 55 kDa) were detected using a polyclonal anti MAO-A/MAO-B antiserum. Western blotting and subsequent densitometric analysis indicate that whole and endocrine pancreas express the two isoforms with different relative proportions. Islets appear to express almost twice as much MAO protein as whole pancreas, in near equal proportions of the two isoforms, whereas whole pancreas expresses more MAO-A than the B isoform. The expression of MAO A and B in islets could be the first step toward the characterization of the functional properties of these enzymes in the endocrine pancreas.
journal_name
Life Scijournal_title
Life sciencesauthors
Pizzinat N,Chan SL,Remaury A,Morgan NG,Parini Adoi
10.1016/s0024-3205(99)00265-9subject
Has Abstractpub_date
1999-01-01 00:00:00pages
441-8issue
4eissn
0024-3205issn
1879-0631pii
S0024320599002659journal_volume
65pub_type
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