Abstract:
:The hypothalamic AMP-activated protein kinase (AMPK)/acetyl-CoA carboxylase (ACC) pathway is known to play an important role in the control of food intake and energy expenditure. Here, we hypothesize that citrate, an intermediate metabolite, activates hypothalamic ACC and is involved in the control of energy mobilization. Initially, we showed that ICV citrate injection decreased food intake and diminished weight gain significantly when compared to control and pair-fed group results. In addition, we showed that intracerebroventricular (ICV) injection of citrate diminished (80% of control) the phosphorylation of ACC, an important AMPK substrate. Furthermore, citrate treatment inhibited (75% of control) hypothalamic AMPK phosphorylation during fasting. In addition to its central effect, ICV citrate injection led to low blood glucose levels during glucose tolerance test (GTT) and high glucose uptake during hyperglycemic-euglycemic clamp. Accordingly, liver glycogen content was higher in animals given citrate (ICV) than in the control group (23.3+/-2.5 vs. 2.7+/-0.5 microg mL(-1) mg(-1), respectively). Interestingly, liver AMPK phosphorylation was reduced (80%) by the citrate treatment. The pharmacological blockade of beta3-adrenergic receptor (SR 59230A) blocked the effect of ICV citrate and citrate plus insulin on liver AMPK phosphorylation. Consistently with these results, rats treated with citrate (ICV) presented improved insulin signal transduction in liver, skeletal muscle, and epididymal fat pad. Similar results were obtained by hypothalamic administration of ARA-A, a competitive inhibitor of AMPK. Our results suggest that the citrate produced by mitochondria may modulate ACC phosphorylation in the hypothalamus, controlling food intake and coordinating a multiorgan network that controls glucose homeostasis and energy uptake through the adrenergic system.
journal_name
Life Scijournal_title
Life sciencesauthors
Cesquini M,Stoppa GR,Prada PO,Torsoni AS,Romanatto T,Souza A,Saad MJ,Velloso LA,Torsoni MAdoi
10.1016/j.lfs.2008.04.015subject
Has Abstractpub_date
2008-06-20 00:00:00pages
1262-71issue
25-26eissn
0024-3205issn
1879-0631pii
S0024-3205(08)00180-Xjournal_volume
82pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::Alzheimer's disease (AD) is the most common form of dementia worldwide. β-amyloid peptide (Aβ) is currently assumed to be the main cause of synaptic dysfunction and cognitive impairments in AD, but the molecular signaling pathways underlying its neurotoxic consequences have not yet been completely explored. Additional...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2020.118020
更新日期:2020-09-15 00:00:00
abstract::The effects of 14-day physical exercise or iloprost treatment (0.5-2 ng/Kg/min) on endogenous nitric oxide production and neutrophil adhesion were evaluated in 20 patients with peripheral arterial occlusive disease (Fontaine Stage II). Peripheral venous blood samples and 4-h urine samples were collected before, immedi...
journal_title:Life sciences
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0024-3205(99)00550-0
更新日期:1999-01-01 00:00:00
abstract::Ventricular hypertrophy is a risk factors for arrhythmias, ischemia and sudden death. It involves cellular modifications leading to a pathological remodeling and is associated with heart failure. The activation of the G protein-coupled estrogen receptor (GPER) mediates beneficial actions in the cardiovascular system. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.117211
更新日期:2020-02-01 00:00:00
abstract::The central interference induced by dexamethasone and RU-38486, (a glucocorticoid receptor antagonist) on morphine antinociception were studied by using the tail flick test in mice. Dexamethasone, injected intracerebrally (i.c.v.) 10 minutes before morphine, dose-dependently reduced morphine antinociception, whereas R...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90521-p
更新日期:1992-01-01 00:00:00
abstract::In order to better understand the mechanisms underlying the reduction in GH secretion in diabetic rats, we have characterized and measured SRIH receptors in the hypothalamus and anterior pituitary gland 5 and 9 days after induction of diabetes in the rat. Experimental diabetes was induced by an intraperitoneal injecti...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)90151-1
更新日期:1994-01-01 00:00:00
abstract:AIMS:Lithium is a common mood stabilizer to treat bipolar disorder. It has a narrow window of therapeutic action and its mechanism of action and possible side effects are still not fully understood. Lithium is a potent inhibitor of glycogen synthase kinase 3β (GSK-3β). Previous studies indicated that lithium can induce...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2012.07.027
更新日期:2012-09-24 00:00:00
abstract::In the present study the effects of Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) on fibroblast growth and activity have been studied. In this regard the AA have evaluated in primary cultures of human gengival normal fibroblasts (PG1 cells): a)-the expression of GM-CSF receptor (GM-CSFR) (alfa unit) on the...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00281-1
更新日期:1998-01-01 00:00:00
abstract::Previous studies showed that acute treatment with aminophylline (AMPY) significantly elevated maximum thermogenesis and improved cold tolerance in rats and man in severe cold. However, the exact mechanism by which AMPY enhances thermogenesis was unknown. Rats receiving enprofylline (ENPRO) (1.5 and 15 mg/kg, i.p.), a ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90491-8
更新日期:1989-01-01 00:00:00
abstract::Based on the finding of decreased mitochondrial complex I activity in the substantia nigra of patients with Parkinson's disease, we propose that the consequent reduction of ATP synthesis and increased generation of reactive oxygen species may be a possible cause of nigrostriatal cell death. Since sulfhydryl groups are...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/s0024-3205(98)00472-x
更新日期:1999-01-01 00:00:00
abstract:AIMS:Asymmetric dimethylarginine (ADMA) is an endogenous competitive inhibitor of nitric oxide (NO) synthase, an enzyme responsible for the generation of NO. Plasma concentrations of ADMA increase in the elderly and in postmenopausal women. In fact, an elevated ADMA level is a risk factor of cardiovascular disease. Aer...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2014.05.003
更新日期:2014-07-11 00:00:00
abstract:AIMS:Aberrant expression of miRNAs exert the critical roles in carcinogenesis, including cervical cancer. Recent study corroborated the down-regulation of miR424-5p in uterine cervix adenocarcinoma. This research aimed to investigate the function and underlying mechanisms of miR424-5p in cervical cancer cell growth. M...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2017.01.006
更新日期:2017-02-15 00:00:00
abstract::In order to investigate the action of ethanol on the anterior pituitary gland, the effect of ethanol on prolactin secretion in vitro was studied. Ethanol significantly increased the in vitro incorporation of 3H-leucine into both prolactin contained within the pituitary gland and that released into the medium. The enha...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90473-9
更新日期:1984-11-05 00:00:00
abstract:AIMS:The mechanisms underlying the changes in blood-brain barrier (BBB) integrity and the generation of seizures in childhood associated with preexisting brain lesions like cortical dysplasia (CD) are poorly understood. We investigated the effects of levetiracetam (LEV) on BBB integrity and the survival during hyperthe...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.09.014
更新日期:2010-11-20 00:00:00
abstract::In fish liver catecholamines bind to beta-adrenoceptors (AR) and increase glucose release via cAMP augmentation. Alpha1-AR have recently been shown to mediate IP3 and Ca2+ elevation in catfish and eel hepatocytes, although their coupling to a physiological response has remained doubtful. We have perifused isolated cat...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(99)00216-7
更新日期:1999-01-01 00:00:00
abstract::Leukocyte interactions with endothelial cell monolayers (ECM) and smooth muscle cells (SMC) play an important role during inflammatory processes. Several studies describe an inhibitory effect of dexamethasone on polymorphonuclear leukocytes (PMNL), endothelial cell function, and interleukin-1 (IL-1) release. Aim of th...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00609-2
更新日期:1999-01-01 00:00:00
abstract::The present study was undertaken to examine whether there was a time-dependent change in the toxic effects of amikacin, an aminoglycoside, on renal functions. Male Wistar rats were maintained under conditions of light from 7 am to 7 pm and dark from 7 pm to 7 am. Amikacin (1.2 g/kg) was injected intraperitoneally to a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(94)00647-4
更新日期:1994-01-01 00:00:00
abstract:AIMS:Numerous studies confirmed the involvement of extracellular vesicles in cancer development and progression. The present study was designed to investigate the glycan composition of ectosomes derived by human cutaneous melanoma (CM) cell lines with the use of lectins. MAIN METHODS:Ectosomes released by primary (WM1...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2018.06.026
更新日期:2018-08-15 00:00:00
abstract::Leu-enkephalin (100 micrograms/kg, i.p.) administered to mice 5 min before training in a one way active avoidance task significantly reduced the number of avoidances observed in the peptide treated animals. This impairing action of Leu-enkephalin was partially attenuated by methylnaloxonium (naloxonium), a quarternary...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(85)90101-8
更新日期:1985-12-23 00:00:00
abstract::The effect of gonadal steroids on the response to analgesia testing was determined in castrated male and female rats and castrated male and female rats treated with testosterone propionate (TP) and estradiol benzoate (EB), respectively. The time to respond to a noxious somatic stimulus in the form of heat was assessed...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90631-0
更新日期:1989-01-01 00:00:00
abstract:AIMS:We recently reported that acute exposure to nicotine vasodilates the renal vasculature of male rats via facilitation of endothelial nitric oxide synthase (eNOS). In this study, we investigated whether this effect of nicotine is sexually dimorphic and the role of estrogen in modulating the nicotine effect. MAIN ME...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.11.009
更新日期:2011-01-17 00:00:00
abstract::The distribution of immunoreactive-dynorphin (ir-Dyn) in isolated subpopulations of bovine adrenal chromaffin cells was examined and compared with that of adrenaline (A), noradrenaline (NA) and ir-Leucine-Enkephalin (ir-Leu-Enk). Using a stepwise bovine serum albumin (BSA) gradient, various populations of catecholamin...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90042-5
更新日期:1983-01-17 00:00:00
abstract::In a partially depolarized guinea pig papillary muscle preparation, BAY K8644 stimulated voltage-operated calcium channels, promoting slow action potentials; this effect was dose-dependent over a concentration range of 3 X 10(-7) M to 3 X 10(-6) M. Isoproterenol and histamine also induced slow action potentials by sti...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90651-x
更新日期:1986-07-28 00:00:00
abstract::The pure estrogen antagonist ICI-182780, at doses above 50 micrograms/kg, effectively inhibited the initiation of embryo implantation in rats when administered on day 4 of pregnancy (day 1 = sperm positive). The same dose inhibited the implantation initiating effect of intravenous 25 ng of estradiol-17 beta in delayed...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90186-7
更新日期:1993-01-01 00:00:00
abstract:AIMS:Reduced cardiac β-adrenoceptors (β-AR) and cardiovascular (CV) dysfunction occur in diabetes mellitus (DM) and can be normalized by insulin. It is unclear how the duration of untreated hyperglycemia prior to intervention impacts insulin's effects. This study assesses insulin's effect on reduced myocardial β-AR and...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.03.024
更新日期:2015-07-01 00:00:00
abstract::Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a cell adhesion and signaling receptor that is expressed on hematopoietic and endothelial cells. PECAM-1 is vital to the regulation of inflammatory responses, as it has been shown to serve a variety of pro-inflammatory and anti-inflammatory functions. Pr...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2010.06.001
更新日期:2010-07-17 00:00:00
abstract::After their discovery, the two known cannabinoid receptors, CB(1) and CB(2), have been the focus of research into the cellular signalling mechanisms of cannabinoids. The initial assessment, mainly derived from expression studies, was that cannabinoids, via G(i/o) proteins, negatively modulate cyclic AMP levels, and ac...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2005.05.055
更新日期:2006-01-02 00:00:00
abstract:AIMS:Antitumour effects of pentapeptide (LH) derived from donkey serum albumin hydrolysates were tested against tumour cells both in vitro and in vivo. The mechanism of LH induced tumour cell apoptosis was investigated. MAIN METHODS:Human promyelocytic leukaemia cells (HL 60) were cultured to observe inhibition in vit...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2013.02.013
更新日期:2013-05-20 00:00:00
abstract:AIM:The present study aimed to understand the relationship between pharmacological activation of mGlu4 receptors and regulation of its gene in the hippocampus. MAIN METHODS:The expression level of the GRM4 gene, encoding mGluR4 receptors, was studied in the hippocampus and frontal cortex of rats after pharmacological ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.03.033
更新日期:2016-05-15 00:00:00
abstract::In situ radioligand binding and quantitative autoradiography have been used to measure the density of striatal D1-like, D2-like, and GABAA receptors in rats treated with haloperidol at 0.01 or 0.1 mg/kg/ day or chlorpromazine, olanzapine or clozapine at 0.1 or 1.0 mg/kg/day for 1, 3 or 7 months. [3H]SCH23390 binding t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(01)01214-0
更新日期:2001-08-03 00:00:00
abstract::Phosphatidylinositol (PI) specific phospholipase C treatment of rabbit platelets caused 95% release of acetylcholinesterase in the supernatant and 4 to 6% hydrolysis of membrane PI in 2 min. Under these conditions there was no cell lysis as monitored by lack of lactate dehydrogenase activity in the medium. The phospho...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(86)90590-4
更新日期:1986-02-24 00:00:00