Abstract：
:In the course of investigating the binding of imipramine to soluble cellular fractions from brain and leukocytes using equilibrium dialysis, an artifactual binding component was produced. On Scatchard and double-reciprocal plots of the data, the component appeared as a homogenous population of sites displaying a binding affinity of 170 nM. The obtained pattern of biphasic interaction bore a marked resemblance to reported Scatchard plots representing the interaction of drugs with bovine serum albumin, and depicting two components of widely differing binding affinity and capacity. The artifact occurred when solutions were transferred after dialysis and before quantitation to intermediate containers, and resulted from binding of 3H-imipramine to the walls of these containers. The latter interaction decreased the concentration of radiolabeled drug in the dialysate but not in the dialyzed solution, and thus mimicked increased imipramine binding to the biological material under study. The effect was particularly pronounced at low drug concentrations, and was particularly pronounced at low drug concentrations, and was prevented by the presence of either proteinaceous material, or of an excess of another basic compound such as methadone. The concentration dependence of the phenomenon led to its appearance as a discrete binding component. The artifact was eliminated either by applying an appropriate correction factor, or by transferring the dialyzed solutions directly into scintillation vials for counting.
journal_name
Life Scijournal_title
Life sciencesauthors
Cullen EI,Medzihradsky Fdoi
10.1016/0024-3205(83)90405-8subject
Has Abstractpub_date
1983-07-11 00:00:00pages
131-40issue
2eissn
0024-3205issn
1879-0631pii
0024-3205(83)90405-8journal_volume
33pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Trimidox (3,4,5-trihydroxybenzohydroxamidoxime), a recently synthesized inhibitor of ribonucleotide reductase (RR), was shown to exert anti-proliferative activities in HL-60 and K562 human leukemia cell lines and to prolong the life span of mice inoculated with L1210 mouse leukemia cells. Here we test whether trimidox...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00901-2
更新日期：2000-11-17 00:00:00
abstract：:Bradykinin receptor stimulation results in G-protein-coupled phospholipase activation, initiating protein kinase C (PKC) stimulation and cytosolic free Ca2+ concentration ([Ca2+]i) rises as signalling pathways. Using Rb+ as a tracer for K+, we have studied the mechanisms involved in bradykinin-stimulated Rb+ influx in...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(96)00530-9
更新日期：1996-01-01 00:00:00
abstract：:Although lipid peroxidation of biological samples may be assessed by different chemical and physical methods, those based on the measurement of malondialdehyde formed from the breakdown of endoperoxides during the last stages of the oxidation of a polyunsaturated fatty acid, appear as the most widely used. Among the v...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(91)90550-u
更新日期：1991-01-01 00:00:00
abstract：AIMS:The purpose of this study was to investigate mechanisms of chronic alcohol-induced cardiac remodeling and dysfunction. We also sought to determine the role of cardiac fibroblasts, which play a dynamic role in cardiac remodeling, in mediating these effects. MAIN METHODS:Adult male Wistar rats were exposed to ethan...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117330
更新日期：2020-03-15 00:00:00
abstract：:Alpha adrenergic agonists and antagonists as clonidine, guanfacine, yohimbine, phenylephrine and prazosin inhibited the [3H]-QNB binding to rat brain cortex muscarinic acetylcholine receptor (mAChR, M-1 subtype), heart (M-2 subtype) and parotid gland homogenate (M-3 subtype) in a dose-dependent competitive fashion. Ki...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90436-u
更新日期：1990-01-01 00:00:00
abstract：:G protein-coupled receptors (GPCRs) are integral membrane proteins that, in response to activation by extracellular stimuli, regulate intracellular second messenger levels via their coupling to heterotrimeric G proteins. GPCR activation also initiates a series of molecular events that leads to G protein-coupled recept...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2003.09.009
更新日期：2003-12-05 00:00:00
abstract：:Thymic and spleen cells were treated in vitro or in vivo with insulin. The in vitro treatments were done with 10(-6), 10(-9), 10(-12) and 10(-15) M concentrations for 30 min and after that histamine, serotonin, endorphin and triiodothyronine (T3) content of the cells were detected by using antibodies to the hormones a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.05.084
更新日期：2006-02-02 00:00:00
abstract：:This study examined the protective role of metallothionein (MT) against pulmonary damage caused by acute exposure to metallic mercury (Hg0) vapor using MT-null and wild-type mice. Both strains of mice were exposed to Hg0 at 6.6 to 7.5 mg/m3 for 4 hr each day for 3 consecutive days. This dosing protocol was lethal to o...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00129-0
更新日期：1999-01-01 00:00:00
abstract：:More than 140 thousands of women suffer from endometrial carcinoma in the worldwide, and over 40 thousand of the patients die before and after in surgery and chemoradiotherapy because of its metastasis. However, its molecular mechanism is much less known compared to other cancers. In this study, we demonstrated that l...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117295
更新日期：2020-02-15 00:00:00
abstract：AIMS:Evidence suggests that hypobaric hypoxia (HH) exposure causes biochemical and molecular level perturbations in brain resulting in associated cognitive dysfunction. However, the possible effect of HH on amygdala and the associated limbic regions based functions remains elusive. Regulated fear expression is essentia...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117555
更新日期：2020-08-01 00:00:00
abstract：:Pulmonary surfactant release from lamellar bodies in alveolar type II cells in response to exogenous and circulating catecholamines is well documented but sympathetic influences from direct nerve stimulation are not as extensively studied. In order to study sympathetic nerve influence on release, ultrastructure of typ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00662-8
更新日期：1994-01-01 00:00:00
abstract：:Calcium ionophores, such as the A23187, cause endothelium-dependent relaxation of arterial strips with intact endothelium, whereas the effect of the ionophore should result from the combination of a relaxation caused by the endothelium-dependent factors and of a contraction of the smooth muscles. In addition, the appl...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00646-9
更新日期：2000-06-23 00:00:00
abstract：:Stonustoxin (SNTX) is a 148 kDa, dimeric, hypotensive and lethal protein factor isolated from the venom of the stonefish Synanceja horrida. SNTX (10-320 ng/ml) progressively causes relaxation of endothelium-intact, phenylephrine (PE)-precontracted rat thoracic aortic rings. The SNTX-induced vasorelaxation was inhibite...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2007.01.058
更新日期：2007-04-10 00:00:00
abstract：AIMS:Benidipine is a dihydropyridine (DHP) derived Ca2+ antagonist, can block triple Ca2+ channels (L, N, and T). It has been used as a safety anti-hypertensive drug because of its long-acting relaxant effect on vascular smooth muscle (VSM). However, whether benidipine has similar pharmacological actions in airway smoo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.04.036
更新日期：2019-06-15 00:00:00
abstract：:Administration of cyclosporine (CS) as an immunosuppressive agent in clinical transplantation is associated with multiple side effects including nephrotoxicity and hypertension. These two effects could be related in that the renal changes may be secondary to alterations in organ blood flow. The present studies investi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90080-4
更新日期：1987-06-29 00:00:00
abstract：:This study was designed to document possible changes in bupivacaine and its main metabolite, des-butyl-bupivacaine (PPX) kinetics in mice after a single 5 mg/kg injection of diltiazem. After a single 20 mg/kg i.p. dose of bupivacaine, kinetic parameters of bupivacaine were not statistically different but the ratio AUC...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00601-6
更新日期：1994-01-01 00:00:00
abstract：OBJECTIVE:This study aims to evaluate the effective of azoles and MTX for patients with invasive candidiasis. METHODS:We used the disk diffusion assay and the checkerboard assay to evaluate the in vitro interactions between MTX and antifungals. In addition, we used the transmission electron microscopy to observe the u...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.116827
更新日期：2019-10-15 00:00:00
abstract：AIMS:Endothelial dysfunction is a key triggering event in the development of cardiovascular diseases and the current study explored this phenomenon in the context of inflammation, apoptosis, reactive oxygen species (ROS) and the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway during chronic diabetes. MAIN M...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2010.03.019
更新日期：2010-06-05 00:00:00
abstract：AIMS:Hepatocellular carcinoma (HCC) is a leading cause of cancer mortality worldwide. Decrease in NKG2D ligand levels and exhaustion of NK cells in HCC patients are major causes of immune escape, high recurrence, poor prognosis, and low overall survival. Enhancing the susceptibility of HCC to NK cells by upregulating N...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117396
更新日期：2020-04-01 00:00:00
abstract：:We examined the direct effect of magnesium ion on aldosterone production by adrenal cells using collagenase-dispersed zona-glomerulosa cells in rats. The effects of magnesium on aldosterone production stimulated by angiotensin II or ACTH were also investigated. Both magnesium sulphate (MgSO4) and magnesium chloride (M...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90327-5
更新日期：1989-01-01 00:00:00
abstract：AIMS:Post-procedural myocardial necrosis manifested by elevated cardiac troponin T (cTnT) often complicates percutaneous coronary intervention (PCI). Plasma pentraxin 3 (PTX3) levels are increased in patients with arterial inflammation and especially unstable angina pectoris (UAP). This study tested whether plasma PTX3...
journal_title：Life sciences
pub_type： 临床试验,杂志文章
doi：10.1016/j.lfs.2013.11.021
更新日期：2014-01-24 00:00:00
abstract：:A variety of milk proteins including lactoferrin, angiogenin-1, alpha-lactalbumin, beta-lactoglobulin, lactoperoxidase, casein and the novel whey proteins lactogenin and glycolactin were tested for inhibitory activity toward human immunodeficiency virus-1 reverse transcriptase (HIV-1 RT), alpha-glucosidase, beta-gluco...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00853-5
更新日期：2000-10-20 00:00:00
abstract：:We have determined the major immunoglobulin isotypes (IgG, IgA, IgM) of antiamebic antibodies induced in the serum and in the large and small intestine after local (oral and rectal) or systemic (intraperitoneal and intramuscular) immunization of mice with glutaraldehyde-fixed Entamoeba histolytica trophozoites (GFT). ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00047-8
更新日期：1999-01-01 00:00:00
abstract：:The effect of chronic cigarette smoke exposure on the activity of rat trachea and lung ornithine decarboxylase (ODC) and s-adenosyl-methionine decarboxylase (AdoMet-DC) was determined. Male rats were exposed once daily for 10 min to mainstream (MS) or different sidestream (SS) dilutions of fresh Kentucky 2R1 cigarette...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90567-3
更新日期：1985-12-09 00:00:00
abstract：:This study analyses possible changes during surgical and chemical castration in the expression and pharmacological characteristics of alpha1-adrenoceptor in adult rat seminal vesicle. Ribonuclease protection assays indicated that alpha1a--was the predominant mRNA, while alpha1b-and alpha1d-adrenoceptor transcripts wer...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.03.011
更新日期：2004-08-06 00:00:00
abstract：:Dialyzed aqueous extracts of plant pollen are widely used in the clinical practice of allergy for diagnostic and therapeutic purposes. The present investigation shows that such allergenic extracts are capable of consuming complement in every human serum, independent of the clinical condition. Complement is engaged by ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00101-x
更新日期：1997-01-01 00:00:00
abstract：AIMS:Coronavirus disease 2019 (COVID-19) has appeared in Wuhan, China but the fast transmission has led to its widespread prevalence in various countries, which has made it a global concern. Another concern is the lack of definitive treatment for this disease. The researchers tried different treatment options which are...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118205
更新日期：2020-10-01 00:00:00
abstract：:There is now some evidence that i) the availability of plasma tryptophan, the precursor of serotonin, is significantly lower in pregnant women at the end of term and the first few days after delivery than in nonpregnant women; and ii) both pregnancy and the early puerperium are accompanied by activation of the inflamm...
journal_title：Life sciences
pub_type： 临床试验,杂志文章
doi：10.1016/s0024-3205(02)01853-2
更新日期：2002-09-06 00:00:00
abstract：:The analgesic effects of bestatin and thiorphan, two enzymatic inhibitors protecting endogenous enkephalins from their degradation, and those of DAGO and deltakephalin, respectively mu and delta opioid peptides, are assessed on the electrical stimulation test of the mouse tail. The relative analgesic potency of DAGO a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90595-7
更新日期：1983-01-01 00:00:00
abstract：:Despite the considerable number of published studies in the field of S-nitrosothiols (RSNO), the determination of these compounds in biological samples still represents an analytical challenge, due to several technical obstacles and often long sample preparation procedures. Other problems derive from the intrinsic lab...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2010.10.024
更新日期：2011-01-17 00:00:00
